Description
This track shows genes targeted by
International Knockout Mouse Consortium (IKMC), a
collaboration to generate a public resource of mouse embryonic stem (ES)
cells containing a null mutation in every gene in the mouse genome.
Gene targets are color-coded by status:
- Green: Reagent(s) Available
- Yellow: In Progress
- Blue: Not Started/On Hold
- Black: Withdrawn/Problematic
The KnockOut Mouse Project Data
Coordination Center (KOMP DCC) is the central database resource
for coordinating mouse gene targeting within IKMC and provides
web-based query and display tools for IKMC data. In addition, the
KOMP DCC website provides a tool for the scientific community to
nominate genes of interest to be knocked out by the KOMP initiative.
IKMC members include
KOMP includes two production centers:
CSD, a collaborative team at the Children's Hospital Oakland Research Institute
(CHORI), the Wellcome Trust Sanger Institute and the University
of California at Davis School of Veterinary Medicine, and
a team at the VelociGene division of Regeneron Pharmaceuticals, Inc.
EUCOMM includes 9 participating institutions.
NorCOMM includes several participating institutions.
Items displayed as a solid box represent the gene regions targeted by
the Regeneron gene knockout strategy. In most cases Regeneron alleles
will be complete null alleles that delete the entire protein coding
sequence of the target gene.
Items displayed as lines connecting short boxes represent the
targeting vector construct strategy used by EuCOMM, NorCOMM, and
CSD. This strategy relies on the identification of a
"critical" exon common to all transcript variants that, when
deleted, creates a frame-shift mutation. The short boxes in the middle
of an item show the vector features that surround the critical exon.
Methods
Using complementary targeting strategies, the IKMC centers
design and create targeting vectors, mutant ES cell lines and, to some
extent, mutant mice, embryos or sperm. Materials are distributed to
the research community.
The KOMP Repository
archives, maintains, and distributes IKMC products. Researchers can
order products and get product information from the
Repository. Researchers can also express interest in products that are
still in the pipeline. They will then receive email notification as
soon as KOMP generated products are available for distribution.
The process for ordering EUCOMM materials can be found
here.
The process for ordering TIGM materials can be found
here.
Information on NorCOMM products and services can be found
here.
Credits
Thanks to the International Knockout Mouse Consortium, and Carol Bult in
particular, for providing these data.
References
Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M, Collins FS, Dove WF, Duyk G, Dymecki S, Eppig
JT et al.
The knockout mouse project.
Nat Genet. 2004 Sep;36(9):921-4.
PMID: 15340423; PMC: PMC2716027
Collins FS, Finnell RH, Rossant J, Wurst W.
A new partner for the international knockout mouse consortium.
Cell. 2007 Apr 20;129(2):235.
PMID: 17448981
International Mouse Knockout Consortium, Collins FS, Rossant J, Wurst W.
A mouse for all reasons.
Cell. 2007 Jan 12;128(1):9-13.
PMID: 17218247
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