Human methylome studies SRP223612 Track Settings
 
Whole Genome Bisulfite sequencing: Allele-specific DNA methylation is increased in cancers and its dense mapping in normal plus neoplastic cells increases the yield of disease-associated regulatory SNPs [Bladder Cell Line, Blood, Bone Marrow, Brain, Breast (Primary Tissue), Breast Cell Line, Cell Line, Liver, Lymph Node, Placenta, Placenta, Maternal]

Track collection: Human methylome studies

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experiment
SRX6890787 
SRX6890788 
SRX6890789 
SRX6890790 
SRX6890791 
SRX6890792 
SRX6890793 
SRX6890794 
SRX6890795 
SRX6890796 
SRX6890797 
SRX6890798 
SRX6890799 
SRX6890800 
SRX6890801 
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SRX6890806 
SRX6890807 
SRX6890808 
SRX6890809 
SRX6890810 
SRX6890811 
SRX6890812 
SRX6890813 
SRX6890814 
SRX6890815 
SRX6890816 
SRX6890819 
SRX6890820 
SRX6890821 
SRX6890822 
SRX6890823 
SRX6890828 
SRX6890829 
SRX6890830 
SRX6890831 
SRX6890832 
SRX6890834 
SRX6890835 
SRX6890836 
SRX6890837 
SRX6890838 
SRX6890840 
SRX6890841 
SRX6890842 
SRX6890843 
SRX6890844 
SRX6890845 
SRX6890846 
SRX6890847 
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SRX6890849 
SRX6890850 
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SRX6890860 
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SRX6890863 
SRX6890864 
SRX7904327 
SRX7904328 
SRX7904329 
SRX7904330 
SRX7904331 
SRX7904332 
SRX7904333 
SRX7904334 
experiment
 All views AMR  HMR  CpG reads  CpG methylation  PMD 
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 SRX6890787  HMR  Breast (Primary Tissue) / SRX6890787 (HMR)   Data format 
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 SRX6890787  CpG methylation  Breast (Primary Tissue) / SRX6890787 (CpG methylation)   Data format 
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 SRX6890788  HMR  Blood / SRX6890788 (HMR)   Data format 
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 SRX6890788  CpG methylation  Blood / SRX6890788 (CpG methylation)   Data format 
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 SRX6890789  CpG methylation  Bone Marrow / SRX6890789 (CpG methylation)   Data format 
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 SRX6890790  CpG methylation  Bone Marrow / SRX6890790 (CpG methylation)   Data format 
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 SRX6890791  CpG methylation  Bone Marrow / SRX6890791 (CpG methylation)   Data format 
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 SRX6890792  HMR  Blood / SRX6890792 (HMR)   Data format 
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 SRX6890792  CpG methylation  Blood / SRX6890792 (CpG methylation)   Data format 
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 SRX6890793  HMR  Blood / SRX6890793 (HMR)   Data format 
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 SRX6890793  CpG methylation  Blood / SRX6890793 (CpG methylation)   Data format 
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 SRX6890794  HMR  Blood / SRX6890794 (HMR)   Data format 
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 SRX6890794  CpG methylation  Blood / SRX6890794 (CpG methylation)   Data format 
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 SRX6890795  HMR  Blood / SRX6890795 (HMR)   Data format 
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 SRX6890795  CpG methylation  Blood / SRX6890795 (CpG methylation)   Data format 
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 SRX6890796  HMR  Blood / SRX6890796 (HMR)   Data format 
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 SRX6890796  CpG methylation  Blood / SRX6890796 (CpG methylation)   Data format 
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 SRX6890797  HMR  Bladder Cell Line / SRX6890797 (HMR)   Data format 
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 SRX6890797  CpG methylation  Bladder Cell Line / SRX6890797 (CpG methylation)   Data format 
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 SRX6890798  HMR  Bladder Cell Line / SRX6890798 (HMR)   Data format 
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 SRX6890798  CpG methylation  Bladder Cell Line / SRX6890798 (CpG methylation)   Data format 
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 SRX6890799  HMR  Liver / SRX6890799 (HMR)   Data format 
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 SRX6890799  CpG methylation  Liver / SRX6890799 (CpG methylation)   Data format 
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 SRX6890800  HMR  Brain / SRX6890800 (HMR)   Data format 
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 SRX6890800  CpG methylation  Brain / SRX6890800 (CpG methylation)   Data format 
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 SRX6890801  HMR  Brain / SRX6890801 (HMR)   Data format 
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 SRX6890801  CpG methylation  Brain / SRX6890801 (CpG methylation)   Data format 
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 SRX6890802  HMR  Brain / SRX6890802 (HMR)   Data format 
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 SRX6890802  CpG methylation  Brain / SRX6890802 (CpG methylation)   Data format 
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 SRX6890804  HMR  Liver / SRX6890804 (HMR)   Data format 
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 SRX6890804  CpG methylation  Liver / SRX6890804 (CpG methylation)   Data format 
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 SRX6890805  HMR  Breast (Primary Tissue) / SRX6890805 (HMR)   Data format 
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 SRX6890805  CpG methylation  Breast (Primary Tissue) / SRX6890805 (CpG methylation)   Data format 
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 SRX6890806  CpG methylation  Cell Line / SRX6890806 (CpG methylation)   Data format 
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 SRX6890807  HMR  Blood / SRX6890807 (HMR)   Data format 
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 SRX6890807  CpG methylation  Blood / SRX6890807 (CpG methylation)   Data format 
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 SRX6890808  HMR  Blood / SRX6890808 (HMR)   Data format 
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 SRX6890808  CpG methylation  Blood / SRX6890808 (CpG methylation)   Data format 
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 SRX6890809  HMR  Breast Cell Line / SRX6890809 (HMR)   Data format 
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 SRX6890809  CpG methylation  Breast Cell Line / SRX6890809 (CpG methylation)   Data format 
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 SRX6890810  HMR  Breast Cell Line / SRX6890810 (HMR)   Data format 
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 SRX6890810  CpG methylation  Breast Cell Line / SRX6890810 (CpG methylation)   Data format 
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 SRX6890811  HMR  Breast Cell Line / SRX6890811 (HMR)   Data format 
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 SRX6890811  CpG methylation  Breast Cell Line / SRX6890811 (CpG methylation)   Data format 
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 SRX6890812  HMR  Blood / SRX6890812 (HMR)   Data format 
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 SRX6890812  CpG methylation  Blood / SRX6890812 (CpG methylation)   Data format 
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 SRX6890813  HMR  Blood / SRX6890813 (HMR)   Data format 
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 SRX6890813  CpG methylation  Blood / SRX6890813 (CpG methylation)   Data format 
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 SRX6890814  HMR  Blood / SRX6890814 (HMR)   Data format 
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 SRX6890814  CpG methylation  Blood / SRX6890814 (CpG methylation)   Data format 
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 SRX6890815  HMR  Blood / SRX6890815 (HMR)   Data format 
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 SRX6890815  CpG methylation  Blood / SRX6890815 (CpG methylation)   Data format 
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 SRX6890816  HMR  Brain / SRX6890816 (HMR)   Data format 
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 SRX6890816  CpG methylation  Brain / SRX6890816 (CpG methylation)   Data format 
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 SRX6890819  HMR  Brain / SRX6890819 (HMR)   Data format 
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 SRX6890819  CpG methylation  Brain / SRX6890819 (CpG methylation)   Data format 
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 SRX6890820  HMR  Liver / SRX6890820 (HMR)   Data format 
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 SRX6890820  CpG methylation  Liver / SRX6890820 (CpG methylation)   Data format 
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 SRX6890821  HMR  Brain / SRX6890821 (HMR)   Data format 
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 SRX6890821  CpG methylation  Brain / SRX6890821 (CpG methylation)   Data format 
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 SRX6890822  HMR  Brain / SRX6890822 (HMR)   Data format 
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 SRX6890822  CpG methylation  Brain / SRX6890822 (CpG methylation)   Data format 
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 SRX6890823  HMR  Brain / SRX6890823 (HMR)   Data format 
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 SRX6890823  CpG methylation  Brain / SRX6890823 (CpG methylation)   Data format 
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 SRX6890828  HMR  Blood / SRX6890828 (HMR)   Data format 
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 SRX6890828  CpG methylation  Blood / SRX6890828 (CpG methylation)   Data format 
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 SRX6890829  HMR  Blood / SRX6890829 (HMR)   Data format 
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 Configure
 SRX6890829  CpG methylation  Blood / SRX6890829 (CpG methylation)   Data format 
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 SRX6890830  CpG methylation  Placenta / SRX6890830 (CpG methylation)   Data format 
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 SRX6890831  CpG methylation  Placenta / SRX6890831 (CpG methylation)   Data format 
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 SRX6890832  HMR  Lymph Node / SRX6890832 (HMR)   Data format 
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 SRX6890832  CpG methylation  Lymph Node / SRX6890832 (CpG methylation)   Data format 
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 SRX6890834  HMR  Blood / SRX6890834 (HMR)   Data format 
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 SRX6890834  CpG methylation  Blood / SRX6890834 (CpG methylation)   Data format 
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 SRX6890835  HMR  Blood / SRX6890835 (HMR)   Data format 
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 SRX6890835  CpG methylation  Blood / SRX6890835 (CpG methylation)   Data format 
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 SRX6890836  HMR  Blood / SRX6890836 (HMR)   Data format 
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 SRX6890836  CpG methylation  Blood / SRX6890836 (CpG methylation)   Data format 
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 SRX6890837  HMR  Blood / SRX6890837 (HMR)   Data format 
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 SRX6890837  CpG methylation  Blood / SRX6890837 (CpG methylation)   Data format 
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 SRX6890838  HMR  Blood / SRX6890838 (HMR)   Data format 
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 SRX6890838  CpG methylation  Blood / SRX6890838 (CpG methylation)   Data format 
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 SRX6890840  HMR  Blood / SRX6890840 (HMR)   Data format 
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 SRX6890840  CpG methylation  Blood / SRX6890840 (CpG methylation)   Data format 
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 SRX6890841  HMR  Blood / SRX6890841 (HMR)   Data format 
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 SRX6890841  CpG methylation  Blood / SRX6890841 (CpG methylation)   Data format 
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 SRX6890842  HMR  Blood / SRX6890842 (HMR)   Data format 
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 SRX6890842  CpG methylation  Blood / SRX6890842 (CpG methylation)   Data format 
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 SRX6890843  HMR  Blood / SRX6890843 (HMR)   Data format 
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 Configure
 SRX6890843  CpG methylation  Blood / SRX6890843 (CpG methylation)   Data format 
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 SRX6890844  HMR  Blood / SRX6890844 (HMR)   Data format 
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 SRX6890844  CpG methylation  Blood / SRX6890844 (CpG methylation)   Data format 
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 SRX6890845  HMR  Blood / SRX6890845 (HMR)   Data format 
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 Configure
 SRX6890845  CpG methylation  Blood / SRX6890845 (CpG methylation)   Data format 
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 SRX6890846  HMR  Blood / SRX6890846 (HMR)   Data format 
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 SRX6890846  CpG methylation  Blood / SRX6890846 (CpG methylation)   Data format 
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 SRX6890847  HMR  Blood / SRX6890847 (HMR)   Data format 
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 SRX6890847  CpG methylation  Blood / SRX6890847 (CpG methylation)   Data format 
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 SRX6890848  HMR  Blood / SRX6890848 (HMR)   Data format 
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 SRX6890848  CpG methylation  Blood / SRX6890848 (CpG methylation)   Data format 
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 SRX6890849  HMR  Blood / SRX6890849 (HMR)   Data format 
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 SRX6890849  CpG methylation  Blood / SRX6890849 (CpG methylation)   Data format 
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 SRX6890850  HMR  Blood / SRX6890850 (HMR)   Data format 
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 SRX6890850  CpG methylation  Blood / SRX6890850 (CpG methylation)   Data format 
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 SRX6890851  HMR  Blood / SRX6890851 (HMR)   Data format 
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 SRX6890851  CpG methylation  Blood / SRX6890851 (CpG methylation)   Data format 
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 SRX6890852  CpG methylation  Lymph Node / SRX6890852 (CpG methylation)   Data format 
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 SRX6890853  CpG methylation  Lymph Node / SRX6890853 (CpG methylation)   Data format 
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 SRX6890854  CpG methylation  Lymph Node / SRX6890854 (CpG methylation)   Data format 
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 SRX6890855  HMR  Brain / SRX6890855 (HMR)   Data format 
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 SRX6890855  CpG methylation  Brain / SRX6890855 (CpG methylation)   Data format 
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 SRX6890856  CpG methylation  Brain / SRX6890856 (CpG methylation)   Data format 
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 SRX6890857  CpG methylation  Brain / SRX6890857 (CpG methylation)   Data format 
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 SRX6890858  CpG methylation  Brain / SRX6890858 (CpG methylation)   Data format 
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 SRX6890859  HMR  Brain / SRX6890859 (HMR)   Data format 
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 SRX6890859  CpG methylation  Brain / SRX6890859 (CpG methylation)   Data format 
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 SRX6890860  CpG methylation  Bone Marrow / SRX6890860 (CpG methylation)   Data format 
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 SRX6890861  CpG methylation  Bone Marrow / SRX6890861 (CpG methylation)   Data format 
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 SRX6890862  CpG methylation  Bone Marrow / SRX6890862 (CpG methylation)   Data format 
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 SRX6890863  HMR  Blood / SRX6890863 (HMR)   Data format 
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 SRX6890863  CpG methylation  Blood / SRX6890863 (CpG methylation)   Data format 
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 SRX6890864  HMR  Blood / SRX6890864 (HMR)   Data format 
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 SRX6890864  CpG methylation  Blood / SRX6890864 (CpG methylation)   Data format 
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 SRX7904327  HMR  Blood / SRX7904327 (HMR)   Data format 
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 SRX7904327  CpG methylation  Blood / SRX7904327 (CpG methylation)   Data format 
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 SRX7904328  HMR  Bone Marrow / SRX7904328 (HMR)   Data format 
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 SRX7904328  CpG methylation  Bone Marrow / SRX7904328 (CpG methylation)   Data format 
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 SRX7904329  HMR  Brain / SRX7904329 (HMR)   Data format 
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 SRX7904329  CpG methylation  Brain / SRX7904329 (CpG methylation)   Data format 
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 SRX7904330  HMR  Blood / SRX7904330 (HMR)   Data format 
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 SRX7904330  CpG methylation  Blood / SRX7904330 (CpG methylation)   Data format 
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 SRX7904331  HMR  Blood / SRX7904331 (HMR)   Data format 
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 SRX7904331  CpG methylation  Blood / SRX7904331 (CpG methylation)   Data format 
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 SRX7904332  HMR  Blood / SRX7904332 (HMR)   Data format 
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 SRX7904332  CpG methylation  Blood / SRX7904332 (CpG methylation)   Data format 
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 SRX7904333  CpG methylation  Placenta, Maternal / SRX7904333 (CpG methylation)   Data format 
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 SRX7904334  CpG methylation  Placenta, Maternal / SRX7904334 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: Whole Genome Bisulfite sequencing: Allele-specific DNA methylation is increased in cancers and its dense mapping in normal plus neoplastic cells increases the yield of disease-associated regulatory SNPs
SRA: SRP223612
GEO: GSE137879
Pubmed: 32594908

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX6890787 Breast (Primary Tissue) 0.738 44.4 66341 1618.1 9823 990.4 3964 27931.1 0.988 GSM4090863: sample_1_breast; Homo sapiens; Bisulfite-Seq
SRX6890788 Blood 0.787 61.0 69524 988.8 4056 1036.3 3723 13894.6 0.950 GSM4090864: sample_10_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890789 Bone Marrow 0.406 46.0 136418 10347.1 4549 1116.4 6823 243022.9 0.989 GSM4090865: sample_100_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890790 Bone Marrow 0.403 48.6 59927 24426.4 59498 1765.4 4689 399231.8 0.989 GSM4090866: sample_101_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890791 Bone Marrow 0.432 55.9 55138 25888.2 51211 1858.9 5010 356261.1 0.989 GSM4090867: sample_102_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890792 Blood 0.792 44.2 72041 935.0 1516 844.0 4349 10263.0 0.988 GSM4090868: sample_11_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890793 Blood 0.807 42.8 72467 953.6 1038 895.0 4665 12276.1 0.989 GSM4090869: sample_12_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890794 Blood 0.818 58.9 67595 1081.3 1698 1067.5 4781 15523.3 0.989 GSM4090870: sample_13_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890795 Blood 0.789 41.5 70129 979.4 2321 942.3 3473 12689.1 0.989 GSM4090871: sample_14_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890796 Blood 0.714 46.2 52818 1036.0 1237 900.3 2075 10872.5 0.989 GSM4090872: sample_15_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890797 Bladder Cell Line 0.702 34.5 98066 1020.6 781 943.4 2301 13300.4 0.987 GSM4090873: sample_16_bladder_epith_cells; Homo sapiens; Bisulfite-Seq
SRX6890798 Bladder Cell Line 0.671 25.4 80983 1581.5 1503 1195.6 3629 169954.8 0.987 GSM4090874: sample_17_bladder_epith_cells; Homo sapiens; Bisulfite-Seq
SRX6890799 Liver 0.752 24.2 49729 1095.8 3585 904.8 2719 20235.4 0.988 GSM4090875: sample_2_liver; Homo sapiens; Bisulfite-Seq
SRX6890800 Brain 0.750 51.8 84524 1366.9 3207 1010.1 4618 31143.1 0.966 GSM4090876: sample_20_brain; Homo sapiens; Bisulfite-Seq
SRX6890801 Brain 0.750 56.4 85075 1325.4 3371 1008.6 4668 30206.0 0.964 GSM4090877: sample_22_brain; Homo sapiens; Bisulfite-Seq
SRX6890802 Brain 0.751 70.5 84949 1393.7 3952 1022.3 4832 30465.4 0.963 GSM4090878: sample_23_brain; Homo sapiens; Bisulfite-Seq
SRX6890804 Liver 0.737 22.7 55797 1090.2 1569 911.7 2996 21721.5 0.986 GSM4090880: sample_3_liver; Homo sapiens; Bisulfite-Seq
SRX6890805 Breast (Primary Tissue) 0.725 48.3 67935 1554.6 8927 997.8 4013 27582.6 0.988 GSM4090881: sample_31_breast; Homo sapiens; Bisulfite-Seq
SRX6890806 Cell Line 0.490 27.2 54171 13745.7 1930 1080.5 1777 966289.4 0.989 GSM4090882: sample_36_GM12878_LCL; Homo sapiens; Bisulfite-Seq
SRX6890807 Blood 0.786 24.4 80231 864.4 1364 1087.1 3933 11056.5 0.988 GSM4090883: sample_37_macrophages_type_1; Homo sapiens; Bisulfite-Seq
SRX6890808 Blood 0.792 20.2 78256 868.1 1228 1092.2 4228 10377.8 0.988 GSM4090884: sample_38_macrophages_type_2; Homo sapiens; Bisulfite-Seq
SRX6890809 Breast Cell Line 0.692 51.0 79793 2515.5 1123 1224.4 2222 408088.1 0.985 GSM4090885: sample_39_mammary_epith_cells; Homo sapiens; Bisulfite-Seq
SRX6890810 Breast Cell Line 0.684 48.5 90502 1271.5 2127 1038.4 4805 29005.7 0.985 GSM4090886: sample_40_mammary_epith_cells; Homo sapiens; Bisulfite-Seq
SRX6890811 Breast Cell Line 0.710 53.5 97593 1127.5 1405 1217.9 4465 17054.4 0.987 GSM4090887: sample_41_mammary_epith_cells; Homo sapiens; Bisulfite-Seq
SRX6890812 Blood 0.755 24.2 78639 842.0 1351 1089.0 3688 9966.9 0.986 GSM4090888: sample_42_monocytes; Homo sapiens; Bisulfite-Seq
SRX6890813 Blood 0.762 51.2 83233 913.5 1593 1088.0 4452 11322.2 0.987 GSM4090889: sample_43_monocytes; Homo sapiens; Bisulfite-Seq
SRX6890814 Blood 0.763 57.3 81760 943.6 1641 1055.2 4209 13825.8 0.988 GSM4090890: sample_44_monocytes; Homo sapiens; Bisulfite-Seq
SRX6890815 Blood 0.773 27.5 74149 877.3 919 868.9 3858 13202.6 0.989 GSM4090891: sample_45_monocytes; Homo sapiens; Bisulfite-Seq
SRX6890816 Brain 0.764 51.8 75775 1466.4 2699 938.3 4353 25109.8 0.984 GSM4090892: sample_46_glial_cells; Homo sapiens; Bisulfite-Seq
SRX6890819 Brain 0.788 46.0 76798 1456.7 2151 942.1 4398 24636.4 0.985 GSM4090895: sample_49_glial_cells; Homo sapiens; Bisulfite-Seq
SRX6890820 Liver 0.740 19.5 49533 1180.5 1715 901.0 2976 24711.2 0.987 GSM4090896: sample_5_liver; Homo sapiens; Bisulfite-Seq
SRX6890821 Brain 0.796 35.0 77368 1620.4 6620 909.7 6431 21772.0 0.942 GSM4090897: sample_50a_neurons; Homo sapiens; Bisulfite-Seq
SRX6890822 Brain 0.806 57.3 69628 1657.6 8691 966.8 5894 22565.0 0.928 GSM4090898: sample_50b_neurons; Homo sapiens; Bisulfite-Seq
SRX6890823 Brain 0.782 28.0 75157 1696.6 8769 948.8 4196 64086.0 0.948 GSM4090899: sample_51a_neurons; Homo sapiens; Bisulfite-Seq
SRX6890828 Blood 0.747 25.3 66103 951.2 1732 973.0 3203 11741.0 0.985 GSM4090904: sample_58_PBL; Homo sapiens; Bisulfite-Seq
SRX6890829 Blood 0.746 42.2 68541 981.9 2538 960.1 3645 12231.7 0.987 GSM4090905: sample_59_PBL; Homo sapiens; Bisulfite-Seq
SRX6890830 Placenta 0.619 33.8 58899 11879.4 9048 1090.7 2675 391110.3 0.983 GSM4090906: sample_62_placenta; Homo sapiens; Bisulfite-Seq
SRX6890831 Placenta 0.575 42.5 81153 11018.1 2106 972.5 4711 215483.2 0.988 GSM4090907: sample_63_cytotrophoblast; Homo sapiens; Bisulfite-Seq
SRX6890832 Lymph Node 0.748 40.9 51638 1188.2 4220 996.5 2481 14681.6 0.987 GSM4090908: sample_64_lymph_node; Homo sapiens; Bisulfite-Seq
SRX6890834 Blood 0.744 43.6 57301 997.9 1635 1028.2 2728 11189.3 0.988 GSM4090910: sample_69_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890835 Blood 0.783 22.0 55804 964.6 754 893.3 3619 9195.4 0.985 GSM4090911: sample_71_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890836 Blood 0.752 18.3 46299 1092.3 982 1073.2 2621 10678.7 0.985 GSM4090912: sample_72_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890837 Blood 0.755 33.1 53574 993.0 2225 1036.1 2958 10797.8 0.983 GSM4090913: sample_75_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890838 Blood 0.784 23.1 61515 975.3 1395 1018.6 3760 11236.0 0.988 GSM4090914: sample_77_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890840 Blood 0.748 42.1 52844 1016.1 3052 975.7 2340 10998.0 0.945 GSM4090916: sample_79_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890841 Blood 0.793 42.9 64483 1009.7 2475 977.9 3821 12541.4 0.989 GSM4090917: sample_7a_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890842 Blood 0.784 43.9 62110 994.8 3849 1013.4 3513 11519.0 0.947 GSM4090918: sample_7b_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890843 Blood 0.773 63.0 69803 963.0 2237 949.5 4353 11104.0 0.978 GSM4090919: sample_8_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890844 Blood 0.764 41.6 53406 1073.2 4472 1034.3 2744 12299.3 0.947 GSM4090920: sample_80_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890845 Blood 0.720 39.8 51937 1064.6 1057 929.8 2087 11562.2 0.987 GSM4090921: sample_81_T_cells; Homo sapiens; Bisulfite-Seq
SRX6890846 Blood 0.793 47.7 63429 1050.8 1746 1030.4 4089 14847.5 0.988 GSM4090922: sample_82_T_cells_CD4; Homo sapiens; Bisulfite-Seq
SRX6890847 Blood 0.789 48.9 63354 1025.1 1459 1041.2 3803 13366.8 0.986 GSM4090923: sample_83_T_cells_CD4; Homo sapiens; Bisulfite-Seq
SRX6890848 Blood 0.737 38.5 54358 1001.3 932 913.1 2456 11188.3 0.987 GSM4090924: sample_84_T_cells_CD4; Homo sapiens; Bisulfite-Seq
SRX6890849 Blood 0.750 46.5 57230 1005.4 1410 1050.8 2835 12083.1 0.987 GSM4090925: sample_85_T_cells_CD4; Homo sapiens; Bisulfite-Seq
SRX6890850 Blood 0.715 27.6 46045 1188.0 964 896.0 1813 12353.2 0.987 GSM4090926: sample_86_T_cells_CD8; Homo sapiens; Bisulfite-Seq
SRX6890851 Blood 0.748 26.6 52527 1022.1 1591 1014.8 2827 10942.7 0.988 GSM4090927: sample_87_T_cells_CD8; Homo sapiens; Bisulfite-Seq
SRX6890852 Lymph Node 0.558 44.8 218753 3533.3 6254 1130.0 3082 478259.4 0.989 GSM4090928: sample_88_DLBCL; Homo sapiens; Bisulfite-Seq
SRX6890853 Lymph Node 0.538 41.0 80356 11773.3 15732 1135.5 4587 355359.3 0.988 GSM4090929: sample_89_DLBCL; Homo sapiens; Bisulfite-Seq
SRX6890854 Lymph Node 0.514 41.9 77115 5134.2 7431 1214.7 1205 1192505.6 0.989 GSM4090930: sample_90_FL; Homo sapiens; Bisulfite-Seq
SRX6890855 Brain 0.701 45.4 60074 1508.4 8545 977.5 4418 96410.2 0.985 GSM4090931: sample_91_GBM; Homo sapiens; Bisulfite-Seq
SRX6890856 Brain 0.676 45.7 75083 5773.4 5046 1042.3 2449 301693.5 0.989 GSM4090932: sample_92_GBM; Homo sapiens; Bisulfite-Seq
SRX6890857 Brain 0.686 22.5 75634 4455.1 2147 1131.8 2219 306195.6 0.985 GSM4090933: sample_94_GBM; Homo sapiens; Bisulfite-Seq
SRX6890858 Brain 0.615 53.5 88207 5808.7 2160 1048.1 3783 231767.9 0.985 GSM4090934: sample_95_GBM; Homo sapiens; Bisulfite-Seq
SRX6890859 Brain 0.743 44.4 60090 1314.8 7563 1025.0 2916 20865.1 0.981 GSM4090935: sample_96_GBM; Homo sapiens; Bisulfite-Seq
SRX6890860 Bone Marrow 0.562 35.8 24558 31930.9 76138 1549.7 2294 734103.0 0.970 GSM4090936: sample_97_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890861 Bone Marrow 0.474 36.6 113536 10992.4 8469 1095.4 4255 408986.9 0.945 GSM4090937: sample_98_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890862 Bone Marrow 0.355 48.7 82721 19772.5 23947 1570.4 5119 364982.2 0.989 GSM4090938: sample_99_myeloma; Homo sapiens; Bisulfite-Seq
SRX6890863 Blood 0.787 45.6 68839 962.0 2055 1000.2 3990 12317.5 0.989 GSM4090939: sample_9a_B_cells; Homo sapiens; Bisulfite-Seq
SRX6890864 Blood 0.786 42.2 67402 984.7 5882 1032.3 2788 13250.0 0.925 GSM4090940: sample_9b_B_cells; Homo sapiens; Bisulfite-Seq
SRX7904327 Blood 0.789 27.0 79071 902.9 1176 1033.3 3741 15203.9 0.990 GSM4409408: sample_103_monocytes; Homo sapiens; Bisulfite-Seq
SRX7904328 Bone Marrow 0.623 32.6 45043 1228.1 23373 1351.3 1348 985332.9 0.990 GSM4409409: sample_104_myeloma; Homo sapiens; Bisulfite-Seq
SRX7904329 Brain 0.791 33.3 65792 972.1 2195 992.6 3272 9569.0 0.990 GSM4409410: sample_105_B_cells; Homo sapiens; Bisulfite-Seq
SRX7904330 Blood 0.688 40.1 81491 2736.4 4110 998.7 2704 262589.9 0.987 GSM4409411: sample_106_GBM; Homo sapiens; Bisulfite-Seq
SRX7904331 Blood 0.802 27.5 80922 898.2 794 876.5 4307 13329.2 0.990 GSM4409412: sample_107_monocytes; Homo sapiens; Bisulfite-Seq
SRX7904332 Blood 0.797 28.7 80838 902.3 1284 1033.6 3806 14990.7 0.990 GSM4409413: sample_108_monocytes; Homo sapiens; Bisulfite-Seq
SRX7904333 Placenta, Maternal 0.610 48.6 72983 11820.7 5009 1008.1 4037 254522.5 0.962 GSM4409414: sample_109_cytotrophoblast_mat; Homo sapiens; Bisulfite-Seq
SRX7904334 Placenta, Maternal 0.501 34.1 55843 15847.3 5943 987.7 3110 439132.4 0.974 GSM4409415: sample_110_extravillous_trophoblast_mat; Homo sapiens; Bisulfite-Seq

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.