Human methylome studies SRP126677 Track Settings
 
Genome-wide profiling at single-nucleotide resolution of brain cell types in schizophrenia [WGBS] [NeuN, Olig2]

Track collection: Human methylome studies

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experiment
SRX3468746 
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experiment
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 SRX3468746  HMR  NeuN / SRX3468746 (HMR)   Data format 
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 SRX3468746  CpG methylation  NeuN / SRX3468746 (CpG methylation)   Data format 
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 SRX3468747  HMR  NeuN / SRX3468747 (HMR)   Data format 
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 SRX3468747  CpG methylation  NeuN / SRX3468747 (CpG methylation)   Data format 
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 SRX3468748  HMR  NeuN / SRX3468748 (HMR)   Data format 
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 SRX3468748  CpG methylation  NeuN / SRX3468748 (CpG methylation)   Data format 
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 SRX3468749  HMR  NeuN / SRX3468749 (HMR)   Data format 
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 SRX3468749  CpG methylation  NeuN / SRX3468749 (CpG methylation)   Data format 
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 SRX3468750  HMR  NeuN / SRX3468750 (HMR)   Data format 
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 SRX3468750  CpG methylation  NeuN / SRX3468750 (CpG methylation)   Data format 
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 SRX3468751  HMR  NeuN / SRX3468751 (HMR)   Data format 
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 SRX3468751  CpG methylation  NeuN / SRX3468751 (CpG methylation)   Data format 
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 SRX3468752  HMR  NeuN / SRX3468752 (HMR)   Data format 
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 SRX3468752  CpG methylation  NeuN / SRX3468752 (CpG methylation)   Data format 
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 SRX3468753  HMR  NeuN / SRX3468753 (HMR)   Data format 
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 SRX3468753  CpG methylation  NeuN / SRX3468753 (CpG methylation)   Data format 
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 SRX3468754  HMR  NeuN / SRX3468754 (HMR)   Data format 
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 SRX3468754  CpG methylation  NeuN / SRX3468754 (CpG methylation)   Data format 
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 SRX3468755  HMR  NeuN / SRX3468755 (HMR)   Data format 
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 SRX3468755  CpG methylation  NeuN / SRX3468755 (CpG methylation)   Data format 
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 SRX3468756  HMR  Olig2 / SRX3468756 (HMR)   Data format 
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 SRX3468756  CpG methylation  Olig2 / SRX3468756 (CpG methylation)   Data format 
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 SRX3468757  HMR  Olig2 / SRX3468757 (HMR)   Data format 
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 SRX3468757  CpG methylation  Olig2 / SRX3468757 (CpG methylation)   Data format 
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 SRX3468758  HMR  NeuN / SRX3468758 (HMR)   Data format 
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 SRX3468758  CpG methylation  NeuN / SRX3468758 (CpG methylation)   Data format 
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 SRX3468759  HMR  NeuN / SRX3468759 (HMR)   Data format 
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 SRX3468759  CpG methylation  NeuN / SRX3468759 (CpG methylation)   Data format 
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 SRX3468760  HMR  NeuN / SRX3468760 (HMR)   Data format 
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 SRX3468760  CpG methylation  NeuN / SRX3468760 (CpG methylation)   Data format 
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 SRX3468761  HMR  Olig2 / SRX3468761 (HMR)   Data format 
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 SRX3468761  CpG methylation  Olig2 / SRX3468761 (CpG methylation)   Data format 
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 SRX3468762  HMR  NeuN / SRX3468762 (HMR)   Data format 
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 SRX3468762  CpG methylation  NeuN / SRX3468762 (CpG methylation)   Data format 
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 SRX3468763  HMR  NeuN / SRX3468763 (HMR)   Data format 
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 SRX3468763  CpG methylation  NeuN / SRX3468763 (CpG methylation)   Data format 
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 SRX3468764  HMR  Olig2 / SRX3468764 (HMR)   Data format 
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 SRX3468764  CpG methylation  Olig2 / SRX3468764 (CpG methylation)   Data format 
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 SRX3468765  HMR  NeuN / SRX3468765 (HMR)   Data format 
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 SRX3468765  CpG methylation  NeuN / SRX3468765 (CpG methylation)   Data format 
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 SRX3468766  HMR  Olig2 / SRX3468766 (HMR)   Data format 
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 SRX3468766  CpG methylation  Olig2 / SRX3468766 (CpG methylation)   Data format 
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 SRX3468767  HMR  Olig2 / SRX3468767 (HMR)   Data format 
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 SRX3468767  CpG methylation  Olig2 / SRX3468767 (CpG methylation)   Data format 
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 SRX3468768  HMR  Olig2 / SRX3468768 (HMR)   Data format 
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 SRX3468768  CpG methylation  Olig2 / SRX3468768 (CpG methylation)   Data format 
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 SRX3468769  HMR  NeuN / SRX3468769 (HMR)   Data format 
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 SRX3468769  CpG methylation  NeuN / SRX3468769 (CpG methylation)   Data format 
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 SRX3468774  HMR  Olig2 / SRX3468774 (HMR)   Data format 
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 SRX3468774  CpG methylation  Olig2 / SRX3468774 (CpG methylation)   Data format 
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 SRX3468775  HMR  NeuN / SRX3468775 (HMR)   Data format 
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 SRX3468775  CpG methylation  NeuN / SRX3468775 (CpG methylation)   Data format 
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 SRX3468776  HMR  NeuN / SRX3468776 (HMR)   Data format 
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 SRX3468776  CpG methylation  NeuN / SRX3468776 (CpG methylation)   Data format 
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 SRX3468777  HMR  NeuN / SRX3468777 (HMR)   Data format 
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 SRX3468777  CpG methylation  NeuN / SRX3468777 (CpG methylation)   Data format 
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 SRX3468778  HMR  NeuN / SRX3468778 (HMR)   Data format 
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 SRX3468778  CpG methylation  NeuN / SRX3468778 (CpG methylation)   Data format 
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 SRX3468779  HMR  NeuN / SRX3468779 (HMR)   Data format 
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 SRX3468779  CpG methylation  NeuN / SRX3468779 (CpG methylation)   Data format 
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 SRX3468780  HMR  NeuN / SRX3468780 (HMR)   Data format 
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 SRX3468780  CpG methylation  NeuN / SRX3468780 (CpG methylation)   Data format 
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 SRX3468781  HMR  NeuN / SRX3468781 (HMR)   Data format 
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 SRX3468781  CpG methylation  NeuN / SRX3468781 (CpG methylation)   Data format 
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 SRX3468782  HMR  NeuN / SRX3468782 (HMR)   Data format 
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 SRX3468782  CpG methylation  NeuN / SRX3468782 (CpG methylation)   Data format 
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 SRX3468783  HMR  Olig2 / SRX3468783 (HMR)   Data format 
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 SRX3468783  CpG methylation  Olig2 / SRX3468783 (CpG methylation)   Data format 
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 SRX3468784  HMR  NeuN / SRX3468784 (HMR)   Data format 
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 SRX3468784  CpG methylation  NeuN / SRX3468784 (CpG methylation)   Data format 
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 SRX3468785  HMR  NeuN / SRX3468785 (HMR)   Data format 
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 SRX3468785  CpG methylation  NeuN / SRX3468785 (CpG methylation)   Data format 
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 SRX3468786  HMR  NeuN / SRX3468786 (HMR)   Data format 
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 SRX3468786  CpG methylation  NeuN / SRX3468786 (CpG methylation)   Data format 
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 SRX3468787  HMR  NeuN / SRX3468787 (HMR)   Data format 
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 SRX3468787  CpG methylation  NeuN / SRX3468787 (CpG methylation)   Data format 
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 SRX3468788  HMR  NeuN / SRX3468788 (HMR)   Data format 
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 SRX3468788  CpG methylation  NeuN / SRX3468788 (CpG methylation)   Data format 
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 SRX3468789  HMR  NeuN / SRX3468789 (HMR)   Data format 
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 SRX3468789  CpG methylation  NeuN / SRX3468789 (CpG methylation)   Data format 
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 SRX3468790  HMR  NeuN / SRX3468790 (HMR)   Data format 
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 SRX3468790  CpG methylation  NeuN / SRX3468790 (CpG methylation)   Data format 
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 SRX3468791  HMR  NeuN / SRX3468791 (HMR)   Data format 
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 SRX3468791  CpG methylation  NeuN / SRX3468791 (CpG methylation)   Data format 
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 SRX3468792  HMR  NeuN / SRX3468792 (HMR)   Data format 
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 SRX3468792  CpG methylation  NeuN / SRX3468792 (CpG methylation)   Data format 
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 SRX3468793  HMR  NeuN / SRX3468793 (HMR)   Data format 
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 SRX3468793  CpG methylation  NeuN / SRX3468793 (CpG methylation)   Data format 
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 SRX3468794  HMR  NeuN / SRX3468794 (HMR)   Data format 
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 SRX3468794  CpG methylation  NeuN / SRX3468794 (CpG methylation)   Data format 
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 SRX3468796  HMR  Olig2 / SRX3468796 (HMR)   Data format 
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 SRX3468796  CpG methylation  Olig2 / SRX3468796 (CpG methylation)   Data format 
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 SRX3468797  HMR  NeuN / SRX3468797 (HMR)   Data format 
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 SRX3468797  CpG methylation  NeuN / SRX3468797 (CpG methylation)   Data format 
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 SRX3468798  HMR  Olig2 / SRX3468798 (HMR)   Data format 
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 SRX3468798  CpG methylation  Olig2 / SRX3468798 (CpG methylation)   Data format 
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 SRX3468799  HMR  NeuN / SRX3468799 (HMR)   Data format 
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 SRX3468799  CpG methylation  NeuN / SRX3468799 (CpG methylation)   Data format 
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 SRX3468800  HMR  Olig2 / SRX3468800 (HMR)   Data format 
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 SRX3468800  CpG methylation  Olig2 / SRX3468800 (CpG methylation)   Data format 
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 SRX3468801  HMR  Olig2 / SRX3468801 (HMR)   Data format 
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 SRX3468801  CpG methylation  Olig2 / SRX3468801 (CpG methylation)   Data format 
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 SRX3468802  HMR  Olig2 / SRX3468802 (HMR)   Data format 
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 SRX3468802  CpG methylation  Olig2 / SRX3468802 (CpG methylation)   Data format 
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 SRX3468803  HMR  Olig2 / SRX3468803 (HMR)   Data format 
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 SRX3468803  CpG methylation  Olig2 / SRX3468803 (CpG methylation)   Data format 
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 SRX3468804  HMR  Olig2 / SRX3468804 (HMR)   Data format 
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 SRX3468804  CpG methylation  Olig2 / SRX3468804 (CpG methylation)   Data format 
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 SRX3468805  HMR  Olig2 / SRX3468805 (HMR)   Data format 
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 SRX3468805  CpG methylation  Olig2 / SRX3468805 (CpG methylation)   Data format 
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 SRX3468807  HMR  NeuN / SRX3468807 (HMR)   Data format 
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 SRX3468807  CpG methylation  NeuN / SRX3468807 (CpG methylation)   Data format 
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 SRX3468808  HMR  Olig2 / SRX3468808 (HMR)   Data format 
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 SRX3468808  CpG methylation  Olig2 / SRX3468808 (CpG methylation)   Data format 
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 SRX3468809  HMR  NeuN / SRX3468809 (HMR)   Data format 
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 SRX3468809  CpG methylation  NeuN / SRX3468809 (CpG methylation)   Data format 
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 SRX3468810  HMR  Olig2 / SRX3468810 (HMR)   Data format 
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 SRX3468810  CpG methylation  Olig2 / SRX3468810 (CpG methylation)   Data format 
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 SRX3468811  HMR  NeuN / SRX3468811 (HMR)   Data format 
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 SRX3468811  CpG methylation  NeuN / SRX3468811 (CpG methylation)   Data format 
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 SRX3468812  HMR  NeuN / SRX3468812 (HMR)   Data format 
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 SRX3468812  CpG methylation  NeuN / SRX3468812 (CpG methylation)   Data format 
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 SRX3468813  HMR  Olig2 / SRX3468813 (HMR)   Data format 
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 SRX3468813  CpG methylation  Olig2 / SRX3468813 (CpG methylation)   Data format 
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 SRX3468814  HMR  Olig2 / SRX3468814 (HMR)   Data format 
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 SRX3468814  CpG methylation  Olig2 / SRX3468814 (CpG methylation)   Data format 
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 SRX3468815  HMR  Olig2 / SRX3468815 (HMR)   Data format 
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 SRX3468815  CpG methylation  Olig2 / SRX3468815 (CpG methylation)   Data format 
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 SRX3468816  HMR  NeuN / SRX3468816 (HMR)   Data format 
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 SRX3468816  CpG methylation  NeuN / SRX3468816 (CpG methylation)   Data format 
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 SRX3468817  HMR  Olig2 / SRX3468817 (HMR)   Data format 
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 SRX3468817  CpG methylation  Olig2 / SRX3468817 (CpG methylation)   Data format 
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 SRX3468818  HMR  Olig2 / SRX3468818 (HMR)   Data format 
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 SRX3468818  CpG methylation  Olig2 / SRX3468818 (CpG methylation)   Data format 
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 SRX3468819  HMR  Olig2 / SRX3468819 (HMR)   Data format 
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 SRX3468819  CpG methylation  Olig2 / SRX3468819 (CpG methylation)   Data format 
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 SRX3468820  HMR  Olig2 / SRX3468820 (HMR)   Data format 
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 SRX3468820  CpG methylation  Olig2 / SRX3468820 (CpG methylation)   Data format 
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 SRX3468821  HMR  Olig2 / SRX3468821 (HMR)   Data format 
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 SRX3468821  CpG methylation  Olig2 / SRX3468821 (CpG methylation)   Data format 
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 SRX3468822  HMR  Olig2 / SRX3468822 (HMR)   Data format 
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 SRX3468822  CpG methylation  Olig2 / SRX3468822 (CpG methylation)   Data format 
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 SRX3468823  HMR  Olig2 / SRX3468823 (HMR)   Data format 
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 SRX3468823  CpG methylation  Olig2 / SRX3468823 (CpG methylation)   Data format 
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 SRX3468824  HMR  Olig2 / SRX3468824 (HMR)   Data format 
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 SRX3468824  CpG methylation  Olig2 / SRX3468824 (CpG methylation)   Data format 
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 SRX3468825  HMR  NeuN / SRX3468825 (HMR)   Data format 
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 SRX3468825  CpG methylation  NeuN / SRX3468825 (CpG methylation)   Data format 
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 SRX3468826  HMR  Olig2 / SRX3468826 (HMR)   Data format 
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 SRX3468826  CpG methylation  Olig2 / SRX3468826 (CpG methylation)   Data format 
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 SRX3468827  HMR  NeuN / SRX3468827 (HMR)   Data format 
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 SRX3468827  CpG methylation  NeuN / SRX3468827 (CpG methylation)   Data format 
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 SRX3468828  HMR  NeuN / SRX3468828 (HMR)   Data format 
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 SRX3468828  CpG methylation  NeuN / SRX3468828 (CpG methylation)   Data format 
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 SRX3468829  HMR  NeuN / SRX3468829 (HMR)   Data format 
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 SRX3468829  CpG methylation  NeuN / SRX3468829 (CpG methylation)   Data format 
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 SRX3468830  HMR  NeuN / SRX3468830 (HMR)   Data format 
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 SRX3468830  CpG methylation  NeuN / SRX3468830 (CpG methylation)   Data format 
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 SRX3468832  HMR  Olig2 / SRX3468832 (HMR)   Data format 
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 SRX3468832  CpG methylation  Olig2 / SRX3468832 (CpG methylation)   Data format 
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 SRX3468833  HMR  Olig2 / SRX3468833 (HMR)   Data format 
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 SRX3468833  CpG methylation  Olig2 / SRX3468833 (CpG methylation)   Data format 
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 SRX3468834  HMR  Olig2 / SRX3468834 (HMR)   Data format 
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 SRX3468834  CpG methylation  Olig2 / SRX3468834 (CpG methylation)   Data format 
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 SRX3468835  HMR  Olig2 / SRX3468835 (HMR)   Data format 
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 SRX3468835  CpG methylation  Olig2 / SRX3468835 (CpG methylation)   Data format 
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 SRX3468836  HMR  Olig2 / SRX3468836 (HMR)   Data format 
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 SRX3468836  CpG methylation  Olig2 / SRX3468836 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: Genome-wide profiling at single-nucleotide resolution of brain cell types in schizophrenia [WGBS]
SRA: SRP126677
GEO: GSE107729
Pubmed: 31288836

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX3468746 NeuN 0.805 17.7 63875 1406.8 11108 994.9 5366 21670.0 0.950 GSM2877162: 1535_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468747 NeuN 0.744 14.5 53131 1268.8 10236 2695.9 4581 18612.8 0.953 GSM2877163: 1516_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468748 NeuN 0.798 13.4 54021 1283.7 6828 949.9 4994 20150.5 0.952 GSM2877164: 1509_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468749 NeuN 0.797 15.6 55355 1258.7 7204 3421.6 4951 20912.2 0.960 GSM2877165: 1519_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468750 NeuN 0.812 13.6 56580 1324.9 6972 945.8 5420 20776.5 0.950 GSM2877166: 1533_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468751 NeuN 0.780 17.0 53202 1288.2 12205 1011.3 4377 20423.0 0.962 GSM2877167: 1537_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468752 NeuN 0.824 29.0 72408 1628.3 9875 940.0 6521 22353.2 0.943 GSM2877168: 1517_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468753 NeuN 0.785 34.0 68840 1489.1 7388 937.7 5107 20145.1 0.943 GSM2877169: 1531_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468754 NeuN 0.763 30.8 71138 1515.7 7249 969.9 5670 19590.9 0.949 GSM2877170: 1534_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468755 NeuN 0.810 30.1 65144 1653.0 14231 1025.6 4932 52028.2 0.953 GSM2877171: 1512_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468756 Olig2 0.785 26.3 77970 1332.0 1609 1025.0 4280 43131.0 0.989 GSM2877172: 1512_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468757 Olig2 0.779 28.7 79921 1308.4 1338 1042.5 4417 43396.4 0.989 GSM2877173: 1523_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468758 NeuN 0.797 25.5 60876 1454.0 14108 1017.3 4883 22259.2 0.951 GSM2877174: 1524_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468759 NeuN 0.816 32.5 71976 1693.5 13194 992.6 5138 48462.7 0.934 GSM2877175: 4336_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468760 NeuN 0.830 34.5 60168 1802.8 20889 1090.1 4706 29311.6 0.904 GSM2877176: 3545_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468761 Olig2 0.799 39.0 77506 1478.2 1508 901.5 4079 30692.7 0.977 GSM2877177: 3545_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468762 NeuN 0.828 38.1 76011 1736.7 13085 978.0 6481 24589.6 0.927 GSM2877178: 3586_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468763 NeuN 0.810 33.0 74245 1860.7 13141 1012.1 4503 63060.4 0.952 GSM2877179: 1505_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468764 Olig2 0.800 37.0 69350 1463.5 5679 972.1 4251 46550.6 0.986 GSM2877180: 1505_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468765 NeuN 0.807 28.9 68697 1613.3 13089 992.3 4714 55203.6 0.947 GSM2877181: 1523_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468766 Olig2 0.793 27.9 80805 1287.1 1098 1079.8 4361 43451.7 0.987 GSM2877182: 4336_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468767 Olig2 0.785 23.1 76574 1229.4 2605 912.4 4402 22789.5 0.990 GSM2877183: 1524_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468768 Olig2 0.798 34.5 84275 1357.9 820 893.9 4319 28170.0 0.987 GSM2877184: 3586_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468769 NeuN 0.815 30.1 74587 1717.8 11127 977.4 4897 55548.9 0.943 GSM2877185: 4361_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468774 Olig2 0.808 39.1 78284 1414.6 1024 891.2 3921 31596.7 0.983 GSM2877190: 1507_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468775 NeuN 0.808 25.1 69766 1626.8 12560 988.6 4258 61643.0 0.954 GSM2877191: 1508_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468776 NeuN 0.820 25.3 66333 1625.4 14229 1018.9 5210 25462.9 0.950 GSM2877192: 1510_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468777 NeuN 0.821 28.5 73799 1692.3 13301 996.8 6047 27861.2 0.949 GSM2877193: 1511_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468778 NeuN 0.821 33.0 67034 1735.3 14955 1033.4 4622 54167.3 0.952 GSM2877194: 1513_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468779 NeuN 0.820 36.5 67174 1732.9 16511 1042.2 5726 24724.0 0.953 GSM2877195: 1514_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468780 NeuN 0.821 34.9 69022 1798.0 13706 1007.5 3998 65705.7 0.953 GSM2877196: 1515_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468781 NeuN 0.817 33.5 71848 1795.6 12863 994.1 4740 57699.6 0.947 GSM2877197: 1518_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468782 NeuN 0.814 33.4 69508 1808.8 14712 1020.9 4460 59770.0 0.950 GSM2877198: 1525_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468783 Olig2 0.803 25.4 79251 1300.3 663 1000.4 3773 30012.2 0.991 GSM2877199: 1511_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468784 NeuN 0.808 31.2 72010 1770.5 13536 997.4 5759 28797.8 0.950 GSM2877200: 1527_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468785 NeuN 0.805 35.4 73770 1783.2 14035 1003.8 4600 57692.0 0.936 GSM2877201: 1532_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468786 NeuN 0.808 30.4 70695 1646.8 13774 996.2 5794 24990.3 0.948 GSM2877202: 1536_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468787 NeuN 0.809 35.1 73704 1700.6 12695 988.7 5051 50227.1 0.944 GSM2877203: 1538_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468788 NeuN 0.808 32.7 69674 1764.5 14073 1006.1 4288 64452.6 0.950 GSM2877204: 1539_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468789 NeuN 0.810 35.0 72658 1728.4 14789 1017.4 5368 48134.7 0.931 GSM2877205: 1541_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468790 NeuN 0.799 40.6 65274 1858.3 19158 1076.3 5240 26513.2 0.951 GSM2877206: 3602_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468791 NeuN 0.819 37.4 68501 1694.3 17095 1014.6 5147 24784.8 0.951 GSM2877207: 3611_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468792 NeuN 0.818 34.9 58519 1654.9 15173 1019.3 4166 55952.5 0.944 GSM2877208: AN03398_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468793 NeuN 0.805 33.3 78125 1763.6 15157 1018.0 5279 48722.4 0.922 GSM2877209: AN15240_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468794 NeuN 0.834 46.6 79170 2210.3 31195 1197.6 5469 31179.5 0.924 GSM2877210: AN16799_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468796 Olig2 0.798 37.4 78557 1385.8 831 896.6 4032 28765.3 0.991 GSM2877212: 4615_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468797 NeuN 0.825 32.6 69534 1624.1 13539 982.9 6308 21486.7 0.947 GSM2877213: AN05483_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468798 Olig2 0.797 33.5 84063 1253.0 720 939.6 4265 24780.6 0.991 GSM2877214: AN05483_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468799 NeuN 0.810 35.6 72628 1763.5 15049 1006.0 6310 24577.0 0.945 GSM2877215: AN10090_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468800 Olig2 0.794 39.1 78730 1433.8 1429 1086.4 4557 41342.1 0.991 GSM2877216: 1532_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468801 Olig2 0.790 37.2 78983 1448.4 1634 1075.2 4367 47139.2 0.991 GSM2877217: 1539_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468802 Olig2 0.791 28.3 87030 1238.4 511 927.3 3193 33580.7 0.990 GSM2877218: AN10090_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468803 Olig2 0.787 43.2 80969 1479.2 1106 900.9 3953 30734.7 0.992 GSM2877219: 1527_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468804 Olig2 0.802 25.7 83674 1279.0 483 946.1 4011 30137.9 0.981 GSM2877220: 1536_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468805 Olig2 0.789 38.2 76258 1389.0 2857 1003.0 5132 33057.0 0.990 GSM2877221: 1538_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468807 NeuN 0.839 25.8 63567 1612.6 10663 961.8 5546 25719.5 0.939 GSM2877223: 4395_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468808 Olig2 0.798 33.3 81835 1332.0 709 923.7 4329 28335.4 0.989 GSM2877224: 4395_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468809 NeuN 0.801 22.4 70541 1571.7 13915 1006.0 5339 25185.1 0.916 GSM2877225: 4504_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468810 Olig2 0.784 27.9 78557 1365.9 1521 1101.8 4197 46844.7 0.991 GSM2877226: AN17799_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468811 NeuN 0.815 40.8 71070 1840.7 14465 997.3 5743 28026.4 0.947 GSM2877227: 4448_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468812 NeuN 0.821 34.6 67713 1636.0 13236 987.1 5801 23784.4 0.948 GSM2877228: 3590_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468813 Olig2 0.807 30.5 78860 1465.2 10871 1081.2 3866 32079.1 0.930 GSM2877229: 3590_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468814 Olig2 0.800 42.0 73579 1469.5 4547 941.4 4137 29746.0 0.980 GSM2877230: 1520_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468815 Olig2 0.795 37.5 83283 1495.9 1435 1036.2 3683 57648.9 0.976 GSM2877231: 1525_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468816 NeuN 0.809 32.1 72650 1830.2 14217 1016.2 4592 59850.0 0.950 GSM2877232: AN17799_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468817 Olig2 0.800 37.7 77852 1483.9 1976 885.0 3975 31473.9 0.970 GSM2877233: 4448_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468818 Olig2 0.792 37.3 76624 1341.3 789 936.5 3904 27876.4 0.990 GSM2877234: 4504_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468819 Olig2 0.801 42.7 80250 1437.9 1068 903.0 4229 27819.7 0.990 GSM2877235: 3602_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468820 Olig2 0.813 43.0 82240 1426.0 5222 971.3 4043 29982.2 0.942 GSM2877236: 3611_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468821 Olig2 0.791 46.0 78546 1532.0 1054 916.0 3822 31969.0 0.989 GSM2877237: 4804_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468822 Olig2 0.793 43.9 80480 1495.5 1635 1060.0 4927 40842.7 0.991 GSM2877238: AN03398_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468823 Olig2 0.785 44.2 79347 1569.2 1706 1071.6 4241 47315.1 0.989 GSM2877239: AN15240_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468824 Olig2 0.783 48.1 82933 1586.4 3501 873.7 3998 29610.8 0.989 GSM2877240: AN16799_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468825 NeuN 0.799 43.7 82802 1785.7 9136 951.3 6588 25334.9 0.947 GSM2877241: 4804_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468826 Olig2 0.795 43.8 81717 1358.9 809 940.3 3776 28084.1 0.986 GSM2877242: Miami0001_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468827 NeuN 0.819 38.5 64824 1723.4 14557 1009.1 5491 26953.8 0.955 GSM2877243: AN09634_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468828 NeuN 0.811 39.3 71366 1783.3 13228 1001.8 5181 48627.2 0.950 GSM2877244: 4730_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468829 NeuN 0.798 29.6 57182 1481.3 11424 961.3 4030 26465.1 0.965 GSM2877245: AN18099_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468830 NeuN 0.813 29.6 71166 1511.9 8888 941.2 6174 21246.8 0.948 GSM2877246: 1521_WGBS_NeuN; Homo sapiens; Bisulfite-Seq
SRX3468832 Olig2 0.795 35.4 79484 1435.7 1716 1052.5 4720 42436.5 0.990 GSM2877248: 1513_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468833 Olig2 0.787 38.7 77852 1362.3 1456 920.4 4204 24851.5 0.988 GSM2877249: 1514_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468834 Olig2 0.794 34.6 80180 1414.0 1072 1098.9 4015 52168.5 0.989 GSM2877250: 1515_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468835 Olig2 0.780 35.6 81198 1450.0 1382 1111.4 3929 53264.2 0.989 GSM2877251: 1518_WGBS_Olig2; Homo sapiens; Bisulfite-Seq
SRX3468836 Olig2 0.806 34.2 78103 1391.6 527 1022.0 3925 30700.8 0.989 GSM2877252: AN18099_WGBS_Olig2; Homo sapiens; Bisulfite-Seq

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.