Human methylome studies SRP332277 Track Settings
 
Methyl-seq Human [Sperm]

Track collection: Human methylome studies

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experiment
SRX11728773 
SRX11728775 
SRX11728777 
SRX11728779 
SRX11728781 
SRX11728783 
SRX11728785 
SRX11728787 
SRX11728789 
SRX11728791 
SRX11728793 
SRX11728795 
SRX11728797 
SRX11728799 
SRX11728801 
SRX11728803 
SRX11728805 
SRX11728807 
SRX11728809 
SRX11728811 
SRX11728813 
SRX11728815 
SRX11728817 
SRX11728819 
SRX11728821 
SRX11728823 
SRX11728825 
SRX11728827 
SRX11728829 
SRX11728831 
SRX11728833 
SRX11728835 
SRX11728837 
SRX11728839 
SRX11728841 
SRX11728843 
SRX11728845 
SRX11728847 
SRX11728849 
SRX11728851 
SRX11728853 
SRX11728855 
SRX11728857 
SRX11728859 
SRX11728861 
SRX11728863 
SRX11728865 
SRX11728867 
SRX11728869 
SRX11728871 
SRX11728873 
SRX11728875 
SRX11728877 
SRX11728879 
SRX11728881 
SRX11728883 
SRX11728885 
SRX11728887 
SRX11728889 
SRX11728891 
SRX11728893 
SRX11728895 
SRX11728897 
SRX11728899 
SRX11728901 
SRX11728903 
SRX11728905 
SRX11728907 
SRX11728909 
SRX11728911 
SRX11728913 
SRX11728915 
SRX11728917 
SRX11728919 
SRX11728921 
SRX11728923 
SRX11728925 
SRX11728927 
SRX11728929 
SRX11728931 
SRX11728933 
SRX11728935 
SRX11728937 
SRX11728939 
SRX11728941 
SRX11728943 
SRX11728945 
SRX11728947 
SRX11728949 
SRX11728951 
SRX11728953 
SRX11728955 
experiment
 All views AMR  CpG reads  HMR  PMD  CpG methylation 
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 SRX11728773  HMR  Sperm / SRX11728773 (HMR)   Data format 
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 SRX11728773  CpG methylation  Sperm / SRX11728773 (CpG methylation)   Data format 
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 SRX11728775  HMR  Sperm / SRX11728775 (HMR)   Data format 
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 SRX11728775  CpG methylation  Sperm / SRX11728775 (CpG methylation)   Data format 
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 SRX11728777  HMR  Sperm / SRX11728777 (HMR)   Data format 
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 SRX11728777  CpG methylation  Sperm / SRX11728777 (CpG methylation)   Data format 
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 SRX11728779  HMR  Sperm / SRX11728779 (HMR)   Data format 
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 SRX11728779  CpG methylation  Sperm / SRX11728779 (CpG methylation)   Data format 
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 SRX11728781  HMR  Sperm / SRX11728781 (HMR)   Data format 
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 SRX11728781  CpG methylation  Sperm / SRX11728781 (CpG methylation)   Data format 
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 SRX11728783  HMR  Sperm / SRX11728783 (HMR)   Data format 
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 SRX11728783  CpG methylation  Sperm / SRX11728783 (CpG methylation)   Data format 
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 SRX11728785  HMR  Sperm / SRX11728785 (HMR)   Data format 
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 SRX11728785  CpG methylation  Sperm / SRX11728785 (CpG methylation)   Data format 
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 SRX11728787  HMR  Sperm / SRX11728787 (HMR)   Data format 
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 SRX11728787  CpG methylation  Sperm / SRX11728787 (CpG methylation)   Data format 
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 SRX11728789  HMR  Sperm / SRX11728789 (HMR)   Data format 
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 SRX11728789  CpG methylation  Sperm / SRX11728789 (CpG methylation)   Data format 
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 SRX11728791  HMR  Sperm / SRX11728791 (HMR)   Data format 
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 SRX11728791  CpG methylation  Sperm / SRX11728791 (CpG methylation)   Data format 
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 SRX11728793  HMR  Sperm / SRX11728793 (HMR)   Data format 
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 SRX11728793  CpG methylation  Sperm / SRX11728793 (CpG methylation)   Data format 
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 SRX11728795  HMR  Sperm / SRX11728795 (HMR)   Data format 
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 Configure
 SRX11728795  CpG methylation  Sperm / SRX11728795 (CpG methylation)   Data format 
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 SRX11728797  HMR  Sperm / SRX11728797 (HMR)   Data format 
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 SRX11728797  CpG methylation  Sperm / SRX11728797 (CpG methylation)   Data format 
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 SRX11728799  HMR  Sperm / SRX11728799 (HMR)   Data format 
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 SRX11728799  CpG methylation  Sperm / SRX11728799 (CpG methylation)   Data format 
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 SRX11728801  HMR  Sperm / SRX11728801 (HMR)   Data format 
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 SRX11728801  CpG methylation  Sperm / SRX11728801 (CpG methylation)   Data format 
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 SRX11728803  HMR  Sperm / SRX11728803 (HMR)   Data format 
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 SRX11728803  CpG methylation  Sperm / SRX11728803 (CpG methylation)   Data format 
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 SRX11728805  HMR  Sperm / SRX11728805 (HMR)   Data format 
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 SRX11728805  CpG methylation  Sperm / SRX11728805 (CpG methylation)   Data format 
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 SRX11728807  HMR  Sperm / SRX11728807 (HMR)   Data format 
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 SRX11728807  CpG methylation  Sperm / SRX11728807 (CpG methylation)   Data format 
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 SRX11728809  HMR  Sperm / SRX11728809 (HMR)   Data format 
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 SRX11728809  CpG methylation  Sperm / SRX11728809 (CpG methylation)   Data format 
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 SRX11728811  HMR  Sperm / SRX11728811 (HMR)   Data format 
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 SRX11728811  CpG methylation  Sperm / SRX11728811 (CpG methylation)   Data format 
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 SRX11728813  HMR  Sperm / SRX11728813 (HMR)   Data format 
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 SRX11728813  CpG methylation  Sperm / SRX11728813 (CpG methylation)   Data format 
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 SRX11728815  HMR  Sperm / SRX11728815 (HMR)   Data format 
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 SRX11728815  CpG methylation  Sperm / SRX11728815 (CpG methylation)   Data format 
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 SRX11728817  HMR  Sperm / SRX11728817 (HMR)   Data format 
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 SRX11728817  CpG methylation  Sperm / SRX11728817 (CpG methylation)   Data format 
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 SRX11728819  HMR  Sperm / SRX11728819 (HMR)   Data format 
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 SRX11728819  CpG methylation  Sperm / SRX11728819 (CpG methylation)   Data format 
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 SRX11728821  HMR  Sperm / SRX11728821 (HMR)   Data format 
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 SRX11728821  CpG methylation  Sperm / SRX11728821 (CpG methylation)   Data format 
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 SRX11728823  HMR  Sperm / SRX11728823 (HMR)   Data format 
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 SRX11728823  CpG methylation  Sperm / SRX11728823 (CpG methylation)   Data format 
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 SRX11728825  HMR  Sperm / SRX11728825 (HMR)   Data format 
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 SRX11728825  CpG methylation  Sperm / SRX11728825 (CpG methylation)   Data format 
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 SRX11728827  HMR  Sperm / SRX11728827 (HMR)   Data format 
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 SRX11728827  CpG methylation  Sperm / SRX11728827 (CpG methylation)   Data format 
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 SRX11728829  HMR  Sperm / SRX11728829 (HMR)   Data format 
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 SRX11728829  CpG methylation  Sperm / SRX11728829 (CpG methylation)   Data format 
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 SRX11728831  HMR  Sperm / SRX11728831 (HMR)   Data format 
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 Configure
 SRX11728831  CpG methylation  Sperm / SRX11728831 (CpG methylation)   Data format 
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 SRX11728833  HMR  Sperm / SRX11728833 (HMR)   Data format 
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 SRX11728833  CpG methylation  Sperm / SRX11728833 (CpG methylation)   Data format 
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 SRX11728835  HMR  Sperm / SRX11728835 (HMR)   Data format 
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 SRX11728835  CpG methylation  Sperm / SRX11728835 (CpG methylation)   Data format 
hide
 SRX11728837  HMR  Sperm / SRX11728837 (HMR)   Data format 
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 Configure
 SRX11728837  CpG methylation  Sperm / SRX11728837 (CpG methylation)   Data format 
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 SRX11728839  HMR  Sperm / SRX11728839 (HMR)   Data format 
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 Configure
 SRX11728839  CpG methylation  Sperm / SRX11728839 (CpG methylation)   Data format 
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 SRX11728841  HMR  Sperm / SRX11728841 (HMR)   Data format 
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 SRX11728841  CpG methylation  Sperm / SRX11728841 (CpG methylation)   Data format 
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 SRX11728843  HMR  Sperm / SRX11728843 (HMR)   Data format 
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 SRX11728843  CpG methylation  Sperm / SRX11728843 (CpG methylation)   Data format 
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 SRX11728845  HMR  Sperm / SRX11728845 (HMR)   Data format 
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 Configure
 SRX11728845  CpG methylation  Sperm / SRX11728845 (CpG methylation)   Data format 
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 SRX11728847  HMR  Sperm / SRX11728847 (HMR)   Data format 
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 SRX11728847  CpG methylation  Sperm / SRX11728847 (CpG methylation)   Data format 
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 SRX11728849  HMR  Sperm / SRX11728849 (HMR)   Data format 
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 Configure
 SRX11728849  CpG methylation  Sperm / SRX11728849 (CpG methylation)   Data format 
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 SRX11728851  HMR  Sperm / SRX11728851 (HMR)   Data format 
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 Configure
 SRX11728851  CpG methylation  Sperm / SRX11728851 (CpG methylation)   Data format 
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 SRX11728853  HMR  Sperm / SRX11728853 (HMR)   Data format 
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 SRX11728853  CpG methylation  Sperm / SRX11728853 (CpG methylation)   Data format 
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 SRX11728855  HMR  Sperm / SRX11728855 (HMR)   Data format 
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 SRX11728855  CpG methylation  Sperm / SRX11728855 (CpG methylation)   Data format 
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 SRX11728857  HMR  Sperm / SRX11728857 (HMR)   Data format 
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 SRX11728857  CpG methylation  Sperm / SRX11728857 (CpG methylation)   Data format 
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 SRX11728859  HMR  Sperm / SRX11728859 (HMR)   Data format 
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 SRX11728859  CpG methylation  Sperm / SRX11728859 (CpG methylation)   Data format 
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 SRX11728861  HMR  Sperm / SRX11728861 (HMR)   Data format 
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 SRX11728861  CpG methylation  Sperm / SRX11728861 (CpG methylation)   Data format 
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 SRX11728863  HMR  Sperm / SRX11728863 (HMR)   Data format 
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 SRX11728863  CpG methylation  Sperm / SRX11728863 (CpG methylation)   Data format 
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 SRX11728865  HMR  Sperm / SRX11728865 (HMR)   Data format 
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 SRX11728865  CpG methylation  Sperm / SRX11728865 (CpG methylation)   Data format 
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 SRX11728867  HMR  Sperm / SRX11728867 (HMR)   Data format 
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 SRX11728867  CpG methylation  Sperm / SRX11728867 (CpG methylation)   Data format 
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 SRX11728869  HMR  Sperm / SRX11728869 (HMR)   Data format 
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 SRX11728869  CpG methylation  Sperm / SRX11728869 (CpG methylation)   Data format 
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 SRX11728871  HMR  Sperm / SRX11728871 (HMR)   Data format 
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 SRX11728871  CpG methylation  Sperm / SRX11728871 (CpG methylation)   Data format 
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 SRX11728873  HMR  Sperm / SRX11728873 (HMR)   Data format 
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 SRX11728873  CpG methylation  Sperm / SRX11728873 (CpG methylation)   Data format 
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 SRX11728875  HMR  Sperm / SRX11728875 (HMR)   Data format 
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 SRX11728875  CpG methylation  Sperm / SRX11728875 (CpG methylation)   Data format 
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 SRX11728877  HMR  Sperm / SRX11728877 (HMR)   Data format 
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 SRX11728877  CpG methylation  Sperm / SRX11728877 (CpG methylation)   Data format 
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 SRX11728879  HMR  Sperm / SRX11728879 (HMR)   Data format 
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 SRX11728879  CpG methylation  Sperm / SRX11728879 (CpG methylation)   Data format 
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 SRX11728881  HMR  Sperm / SRX11728881 (HMR)   Data format 
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 Configure
 SRX11728881  CpG methylation  Sperm / SRX11728881 (CpG methylation)   Data format 
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 SRX11728883  HMR  Sperm / SRX11728883 (HMR)   Data format 
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 Configure
 SRX11728883  CpG methylation  Sperm / SRX11728883 (CpG methylation)   Data format 
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 SRX11728885  HMR  Sperm / SRX11728885 (HMR)   Data format 
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 SRX11728885  CpG methylation  Sperm / SRX11728885 (CpG methylation)   Data format 
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 SRX11728887  HMR  Sperm / SRX11728887 (HMR)   Data format 
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 SRX11728887  CpG methylation  Sperm / SRX11728887 (CpG methylation)   Data format 
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 SRX11728889  HMR  Sperm / SRX11728889 (HMR)   Data format 
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 SRX11728889  CpG methylation  Sperm / SRX11728889 (CpG methylation)   Data format 
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 SRX11728891  HMR  Sperm / SRX11728891 (HMR)   Data format 
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 Configure
 SRX11728891  CpG methylation  Sperm / SRX11728891 (CpG methylation)   Data format 
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 SRX11728893  HMR  Sperm / SRX11728893 (HMR)   Data format 
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 Configure
 SRX11728893  CpG methylation  Sperm / SRX11728893 (CpG methylation)   Data format 
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 SRX11728895  HMR  Sperm / SRX11728895 (HMR)   Data format 
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 Configure
 SRX11728895  CpG methylation  Sperm / SRX11728895 (CpG methylation)   Data format 
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 SRX11728897  HMR  Sperm / SRX11728897 (HMR)   Data format 
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 Configure
 SRX11728897  CpG methylation  Sperm / SRX11728897 (CpG methylation)   Data format 
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 SRX11728899  HMR  Sperm / SRX11728899 (HMR)   Data format 
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 Configure
 SRX11728899  CpG methylation  Sperm / SRX11728899 (CpG methylation)   Data format 
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 SRX11728901  HMR  Sperm / SRX11728901 (HMR)   Data format 
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 Configure
 SRX11728901  CpG methylation  Sperm / SRX11728901 (CpG methylation)   Data format 
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 SRX11728903  HMR  Sperm / SRX11728903 (HMR)   Data format 
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 SRX11728903  CpG methylation  Sperm / SRX11728903 (CpG methylation)   Data format 
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 SRX11728905  HMR  Sperm / SRX11728905 (HMR)   Data format 
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 SRX11728905  CpG methylation  Sperm / SRX11728905 (CpG methylation)   Data format 
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 SRX11728907  HMR  Sperm / SRX11728907 (HMR)   Data format 
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 SRX11728907  CpG methylation  Sperm / SRX11728907 (CpG methylation)   Data format 
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 SRX11728909  HMR  Sperm / SRX11728909 (HMR)   Data format 
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 SRX11728909  CpG methylation  Sperm / SRX11728909 (CpG methylation)   Data format 
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 SRX11728911  HMR  Sperm / SRX11728911 (HMR)   Data format 
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 SRX11728911  CpG methylation  Sperm / SRX11728911 (CpG methylation)   Data format 
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 SRX11728913  HMR  Sperm / SRX11728913 (HMR)   Data format 
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 SRX11728913  CpG methylation  Sperm / SRX11728913 (CpG methylation)   Data format 
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 SRX11728915  HMR  Sperm / SRX11728915 (HMR)   Data format 
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 SRX11728915  CpG methylation  Sperm / SRX11728915 (CpG methylation)   Data format 
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 SRX11728917  HMR  Sperm / SRX11728917 (HMR)   Data format 
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 SRX11728917  CpG methylation  Sperm / SRX11728917 (CpG methylation)   Data format 
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 SRX11728919  HMR  Sperm / SRX11728919 (HMR)   Data format 
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 SRX11728919  CpG methylation  Sperm / SRX11728919 (CpG methylation)   Data format 
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 SRX11728921  HMR  Sperm / SRX11728921 (HMR)   Data format 
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 SRX11728921  CpG methylation  Sperm / SRX11728921 (CpG methylation)   Data format 
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 SRX11728923  HMR  Sperm / SRX11728923 (HMR)   Data format 
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 SRX11728923  CpG methylation  Sperm / SRX11728923 (CpG methylation)   Data format 
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 SRX11728925  HMR  Sperm / SRX11728925 (HMR)   Data format 
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 SRX11728925  CpG methylation  Sperm / SRX11728925 (CpG methylation)   Data format 
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 SRX11728927  HMR  Sperm / SRX11728927 (HMR)   Data format 
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 SRX11728927  CpG methylation  Sperm / SRX11728927 (CpG methylation)   Data format 
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 SRX11728929  HMR  Sperm / SRX11728929 (HMR)   Data format 
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 SRX11728929  CpG methylation  Sperm / SRX11728929 (CpG methylation)   Data format 
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 SRX11728931  HMR  Sperm / SRX11728931 (HMR)   Data format 
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 SRX11728931  CpG methylation  Sperm / SRX11728931 (CpG methylation)   Data format 
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 SRX11728933  HMR  Sperm / SRX11728933 (HMR)   Data format 
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 SRX11728933  CpG methylation  Sperm / SRX11728933 (CpG methylation)   Data format 
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 SRX11728935  HMR  Sperm / SRX11728935 (HMR)   Data format 
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 SRX11728935  CpG methylation  Sperm / SRX11728935 (CpG methylation)   Data format 
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 SRX11728937  HMR  Sperm / SRX11728937 (HMR)   Data format 
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 SRX11728937  CpG methylation  Sperm / SRX11728937 (CpG methylation)   Data format 
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 SRX11728939  HMR  Sperm / SRX11728939 (HMR)   Data format 
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 SRX11728939  CpG methylation  Sperm / SRX11728939 (CpG methylation)   Data format 
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 SRX11728941  HMR  Sperm / SRX11728941 (HMR)   Data format 
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 SRX11728941  CpG methylation  Sperm / SRX11728941 (CpG methylation)   Data format 
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 SRX11728943  HMR  Sperm / SRX11728943 (HMR)   Data format 
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 SRX11728943  CpG methylation  Sperm / SRX11728943 (CpG methylation)   Data format 
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 SRX11728945  HMR  Sperm / SRX11728945 (HMR)   Data format 
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 SRX11728945  CpG methylation  Sperm / SRX11728945 (CpG methylation)   Data format 
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 SRX11728947  HMR  Sperm / SRX11728947 (HMR)   Data format 
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 SRX11728947  CpG methylation  Sperm / SRX11728947 (CpG methylation)   Data format 
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 SRX11728949  HMR  Sperm / SRX11728949 (HMR)   Data format 
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 SRX11728949  CpG methylation  Sperm / SRX11728949 (CpG methylation)   Data format 
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 SRX11728951  HMR  Sperm / SRX11728951 (HMR)   Data format 
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 SRX11728951  CpG methylation  Sperm / SRX11728951 (CpG methylation)   Data format 
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 SRX11728953  HMR  Sperm / SRX11728953 (HMR)   Data format 
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 SRX11728953  CpG methylation  Sperm / SRX11728953 (CpG methylation)   Data format 
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 SRX11728955  HMR  Sperm / SRX11728955 (HMR)   Data format 
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 SRX11728955  CpG methylation  Sperm / SRX11728955 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: Methyl-seq Human
SRA: SRP332277
GEO: not found
Pubmed: not found

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX11728773 Sperm 0.617 38.7 89567 2360.4 13222 934.1 3863 36097.3 0.997 WGBS human sperm
SRX11728775 Sperm 0.605 30.4 95010 2359.5 11180 938.5 4297 37266.8 0.997 WGBS human sperm
SRX11728777 Sperm 0.620 32.7 90466 2281.7 9749 908.7 4552 31390.7 0.997 WGBS human sperm
SRX11728779 Sperm 0.663 30.0 90708 2543.6 7922 918.3 4842 37797.6 0.997 WGBS human sperm
SRX11728781 Sperm 0.568 25.7 86858 2335.5 21069 1051.5 4535 36632.4 0.996 WGBS human sperm
SRX11728783 Sperm 0.614 33.4 95708 2236.1 13260 953.5 4276 33141.8 0.996 WGBS human sperm
SRX11728785 Sperm 0.623 22.0 90303 2155.7 8471 980.5 1491 88001.8 0.997 WGBS human sperm
SRX11728787 Sperm 0.567 34.7 92230 2276.3 13563 921.5 3973 33692.5 0.997 WGBS human sperm
SRX11728789 Sperm 0.606 36.2 91000 2270.7 13206 978.8 4275 36558.2 0.997 WGBS human sperm
SRX11728791 Sperm 0.689 29.7 101742 2383.0 5741 894.3 5063 33995.7 0.997 WGBS human sperm
SRX11728793 Sperm 0.582 37.8 91570 2154.9 9400 908.1 4187 33765.7 0.997 WGBS human sperm
SRX11728795 Sperm 0.688 24.2 91322 2253.3 7691 918.4 6026 26798.6 0.997 WGBS human sperm
SRX11728797 Sperm 0.648 27.9 90232 2306.2 8468 905.6 4770 31191.4 0.997 WGBS human sperm
SRX11728799 Sperm 0.582 20.3 79522 2140.8 10935 946.3 1621 75015.3 0.996 WGBS human sperm
SRX11728801 Sperm 0.567 7.8 75306 2445.4 3385 901.9 1138 103941.9 0.990 WGBS human sperm
SRX11728803 Sperm 0.666 31.7 95593 2374.3 6642 905.8 5334 32790.6 0.997 WGBS human sperm
SRX11728805 Sperm 0.573 40.3 91145 2361.1 13015 919.0 3618 41317.9 0.997 WGBS human sperm
SRX11728807 Sperm 0.618 37.7 99671 2292.7 12119 954.5 4323 36949.3 0.997 WGBS human sperm
SRX11728809 Sperm 0.604 32.7 90983 2148.1 17580 1012.1 4299 33302.4 0.996 WGBS human sperm
SRX11728811 Sperm 0.611 31.5 91506 2216.8 9892 914.9 4410 33843.7 0.997 WGBS human sperm
SRX11728813 Sperm 0.618 28.7 84348 2076.6 13433 970.6 4905 28231.0 0.996 WGBS human sperm
SRX11728815 Sperm 0.689 25.3 92874 2503.6 5578 875.8 5748 28293.4 0.997 WGBS human sperm
SRX11728817 Sperm 0.590 37.4 91084 2116.9 21247 1029.5 4158 36244.9 0.996 WGBS human sperm
SRX11728819 Sperm 0.643 17.1 85364 2383.6 6678 908.3 4568 37058.8 0.997 WGBS human sperm
SRX11728821 Sperm 0.645 33.8 98421 2407.9 10599 932.7 4035 38202.9 0.997 WGBS human sperm
SRX11728823 Sperm 0.696 25.9 94210 2584.8 8442 936.1 6109 31534.6 0.997 WGBS human sperm
SRX11728825 Sperm 0.696 16.3 89027 2406.2 3978 875.3 4865 35022.6 0.997 WGBS human sperm
SRX11728827 Sperm 0.673 26.8 96317 2440.4 7535 899.4 4809 33595.4 0.997 WGBS human sperm
SRX11728829 Sperm 0.684 23.7 97054 2387.9 6559 916.2 5275 31266.2 0.997 WGBS human sperm
SRX11728831 Sperm 0.583 24.9 83390 2060.7 5966 959.9 1606 89228.8 0.996 WGBS human sperm
SRX11728833 Sperm 0.665 29.9 95748 2555.5 8083 913.8 5082 37793.9 0.997 WGBS human sperm
SRX11728835 Sperm 0.609 32.3 89594 2174.7 10142 965.6 2135 73457.8 0.997 WGBS human sperm
SRX11728837 Sperm 0.608 36.9 98021 2293.5 12097 933.9 3431 41800.3 0.997 WGBS human sperm
SRX11728839 Sperm 0.649 15.7 89025 2416.2 8060 934.9 2226 70416.2 0.997 WGBS human sperm
SRX11728841 Sperm 0.669 30.7 90896 2643.3 7683 918.2 5259 35982.6 0.997 WGBS human sperm
SRX11728843 Sperm 0.677 37.6 95432 2578.2 4528 864.2 4695 38707.8 0.997 WGBS human sperm
SRX11728845 Sperm 0.631 38.0 96148 2429.4 8427 907.4 4809 33125.2 0.997 WGBS human sperm
SRX11728847 Sperm 0.688 30.7 97453 2379.4 6364 917.3 5930 28544.2 0.997 WGBS human sperm
SRX11728849 Sperm 0.691 33.3 95207 2611.0 5728 889.3 4524 41366.3 0.997 WGBS human sperm
SRX11728851 Sperm 0.628 24.2 85189 2021.2 8709 1002.2 1756 85241.4 0.997 WGBS human sperm
SRX11728853 Sperm 0.700 32.9 90960 2553.6 8558 917.4 4715 35486.3 0.997 WGBS human sperm
SRX11728855 Sperm 0.687 27.5 91075 2494.7 8110 904.9 5039 32496.8 0.997 WGBS human sperm
SRX11728857 Sperm 0.652 36.6 99458 2488.8 7485 905.7 4297 37744.8 0.997 WGBS human sperm
SRX11728859 Sperm 0.676 26.4 90003 2541.7 7591 910.0 5722 30067.4 0.997 WGBS human sperm
SRX11728861 Sperm 0.677 23.2 97894 2612.5 5970 884.2 5290 35932.3 0.997 WGBS human sperm
SRX11728863 Sperm 0.693 31.8 101859 2600.6 7140 881.6 5258 37182.7 0.997 WGBS human sperm
SRX11728865 Sperm 0.621 17.2 90768 2388.7 6441 877.9 1975 79504.0 0.997 WGBS human sperm
SRX11728867 Sperm 0.698 27.4 90778 2614.1 6534 889.2 5107 35686.6 0.997 WGBS human sperm
SRX11728869 Sperm 0.704 33.5 89870 2716.1 8007 903.3 5207 33771.5 0.997 WGBS human sperm
SRX11728871 Sperm 0.693 33.1 96313 2650.5 7453 907.4 5269 34657.1 0.997 WGBS human sperm
SRX11728873 Sperm 0.717 32.6 90680 2655.4 8280 908.4 5931 30078.9 0.997 WGBS human sperm
SRX11728875 Sperm 0.648 33.1 98805 2522.2 8080 907.8 4346 37647.4 0.997 WGBS human sperm
SRX11728877 Sperm 0.681 28.1 91584 2402.5 10108 971.6 5326 33649.9 0.997 WGBS human sperm
SRX11728879 Sperm 0.698 28.8 92223 2550.7 8380 929.7 5244 33266.6 0.997 WGBS human sperm
SRX11728881 Sperm 0.582 24.3 87036 2190.9 8420 990.2 1910 85714.5 0.997 WGBS human sperm
SRX11728883 Sperm 0.619 41.9 96240 2353.5 8551 931.9 4140 37962.2 0.997 WGBS human sperm
SRX11728885 Sperm 0.639 26.7 88764 2405.5 10590 953.5 5541 31382.6 0.997 WGBS human sperm
SRX11728887 Sperm 0.612 28.4 95162 2255.1 6565 909.9 3883 35904.3 0.997 WGBS human sperm
SRX11728889 Sperm 0.661 21.6 93304 2287.5 7160 933.9 2258 65966.1 0.997 WGBS human sperm
SRX11728891 Sperm 0.654 32.7 92033 2492.1 6097 894.6 4699 36144.1 0.997 WGBS human sperm
SRX11728893 Sperm 0.695 20.2 90376 2355.7 6845 890.6 5194 29265.2 0.997 WGBS human sperm
SRX11728895 Sperm 0.683 29.3 92067 2470.7 9957 951.6 5079 33504.5 0.997 WGBS human sperm
SRX11728897 Sperm 0.718 20.1 95486 2721.8 6617 901.4 5536 38354.3 0.997 WGBS human sperm
SRX11728899 Sperm 0.689 24.3 96229 2537.4 6557 896.9 5135 33773.3 0.997 WGBS human sperm
SRX11728901 Sperm 0.662 26.4 89525 2525.0 8187 917.7 5450 32785.0 0.997 WGBS human sperm
SRX11728903 Sperm 0.658 29.5 95850 2484.8 6381 895.9 5114 33048.1 0.997 WGBS human sperm
SRX11728905 Sperm 0.674 24.4 92393 2468.9 5817 885.5 5285 33666.6 0.997 WGBS human sperm
SRX11728907 Sperm 0.586 25.7 88114 2321.3 18494 914.9 3884 35586.7 0.997 WGBS human sperm
SRX11728909 Sperm 0.676 28.2 98204 2545.4 6466 894.4 5783 29980.4 0.997 WGBS human sperm
SRX11728911 Sperm 0.650 26.0 87305 2395.0 8837 945.7 5376 28649.2 0.997 WGBS human sperm
SRX11728913 Sperm 0.608 31.8 93669 2204.5 9710 949.0 5265 30719.9 0.997 WGBS human sperm
SRX11728915 Sperm 0.694 28.4 97494 2603.2 8144 902.2 5239 34250.2 0.997 WGBS human sperm
SRX11728917 Sperm 0.692 21.4 93235 2394.2 8164 914.9 5145 34302.9 0.997 WGBS human sperm
SRX11728919 Sperm 0.690 38.3 92894 2616.7 10417 971.2 5511 32342.4 0.997 WGBS human sperm
SRX11728921 Sperm 0.665 29.0 91495 2350.7 9015 923.6 4643 32282.5 0.997 WGBS human sperm
SRX11728923 Sperm 0.718 3.8 72528 2302.4 2150 929.0 1047 125865.4 0.996 WGBS human sperm
SRX11728925 Sperm 0.680 18.8 86770 2252.5 7126 919.5 1805 70976.8 0.997 WGBS human sperm
SRX11728927 Sperm 0.651 7.9 74404 2403.9 4802 894.5 1122 107266.9 0.996 WGBS human sperm
SRX11728929 Sperm 0.654 29.5 95836 2353.5 10522 963.4 5287 32003.8 0.997 WGBS human sperm
SRX11728931 Sperm 0.682 29.7 94925 2453.7 7510 911.2 5247 29264.5 0.997 WGBS human sperm
SRX11728933 Sperm 0.683 24.5 96589 2561.0 8606 919.9 5421 32652.0 0.997 WGBS human sperm
SRX11728935 Sperm 0.669 20.7 90217 2166.0 9465 970.8 2346 65865.2 0.997 WGBS human sperm
SRX11728937 Sperm 0.618 43.4 93249 2445.1 10427 927.7 4414 35675.6 0.997 WGBS human sperm
SRX11728939 Sperm 0.642 35.9 91922 2302.1 9636 929.7 4562 32199.8 0.997 WGBS human sperm
SRX11728941 Sperm 0.706 23.2 92043 2374.7 7938 921.5 5834 29700.8 0.997 WGBS human sperm
SRX11728943 Sperm 0.649 19.9 83366 2237.0 7642 871.2 3331 39152.3 0.996 WGBS human sperm
SRX11728945 Sperm 0.685 24.1 88289 2522.0 8637 941.5 5809 29046.4 0.997 WGBS human sperm
SRX11728947 Sperm 0.593 40.4 96646 2408.0 9312 897.2 4559 37327.2 0.997 WGBS human sperm
SRX11728949 Sperm 0.564 33.7 98315 2184.1 14759 950.3 3281 45842.4 0.997 WGBS human sperm
SRX11728951 Sperm 0.659 29.7 95850 2509.7 6693 876.6 5211 35446.1 0.997 WGBS human sperm
SRX11728953 Sperm 0.651 22.0 88698 2259.6 10882 954.1 1874 70591.2 0.997 WGBS human sperm
SRX11728955 Sperm 0.585 50.5 90273 2439.6 14539 921.3 3581 43538.0 0.997 WGBS human sperm

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.