Human methylome studies SRP323990 Track Settings
 
Identification of DNA Methylation Predicts Onset of PTSD and Depression Following Trauma [Peripheral Blood Mononuclear Cells]

Track collection: Human methylome studies

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SRX11140395 
SRX11140396 
SRX11140397 
SRX11140398 
SRX11140399 
SRX11140400 
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SRX11140457 
SRX11140458 
SRX11140459 
SRX11140460 
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 SRX11140395  HMR  Peripheral Blood Mononuclear Cells / SRX11140395 (HMR)   Data format 
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 SRX11140395  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140395 (CpG methylation)   Data format 
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 SRX11140396  HMR  Peripheral Blood Mononuclear Cells / SRX11140396 (HMR)   Data format 
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 SRX11140396  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140396 (CpG methylation)   Data format 
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 SRX11140397  HMR  Peripheral Blood Mononuclear Cells / SRX11140397 (HMR)   Data format 
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 SRX11140397  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140397 (CpG methylation)   Data format 
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 SRX11140398  HMR  Peripheral Blood Mononuclear Cells / SRX11140398 (HMR)   Data format 
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 SRX11140398  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140398 (CpG methylation)   Data format 
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 SRX11140399  HMR  Peripheral Blood Mononuclear Cells / SRX11140399 (HMR)   Data format 
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 SRX11140399  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140399 (CpG methylation)   Data format 
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 SRX11140400  HMR  Peripheral Blood Mononuclear Cells / SRX11140400 (HMR)   Data format 
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 SRX11140400  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140400 (CpG methylation)   Data format 
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 SRX11140401  HMR  Peripheral Blood Mononuclear Cells / SRX11140401 (HMR)   Data format 
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 SRX11140401  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140401 (CpG methylation)   Data format 
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 SRX11140402  HMR  Peripheral Blood Mononuclear Cells / SRX11140402 (HMR)   Data format 
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 SRX11140402  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140402 (CpG methylation)   Data format 
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 SRX11140403  HMR  Peripheral Blood Mononuclear Cells / SRX11140403 (HMR)   Data format 
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 SRX11140403  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140403 (CpG methylation)   Data format 
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 SRX11140404  HMR  Peripheral Blood Mononuclear Cells / SRX11140404 (HMR)   Data format 
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 SRX11140404  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140404 (CpG methylation)   Data format 
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 SRX11140405  HMR  Peripheral Blood Mononuclear Cells / SRX11140405 (HMR)   Data format 
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 SRX11140405  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140405 (CpG methylation)   Data format 
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 SRX11140406  HMR  Peripheral Blood Mononuclear Cells / SRX11140406 (HMR)   Data format 
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 SRX11140406  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140406 (CpG methylation)   Data format 
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 SRX11140407  HMR  Peripheral Blood Mononuclear Cells / SRX11140407 (HMR)   Data format 
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 SRX11140407  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140407 (CpG methylation)   Data format 
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 SRX11140408  HMR  Peripheral Blood Mononuclear Cells / SRX11140408 (HMR)   Data format 
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 SRX11140408  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140408 (CpG methylation)   Data format 
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 SRX11140409  HMR  Peripheral Blood Mononuclear Cells / SRX11140409 (HMR)   Data format 
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 SRX11140409  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140409 (CpG methylation)   Data format 
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 SRX11140410  HMR  Peripheral Blood Mononuclear Cells / SRX11140410 (HMR)   Data format 
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 SRX11140410  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140410 (CpG methylation)   Data format 
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 SRX11140411  HMR  Peripheral Blood Mononuclear Cells / SRX11140411 (HMR)   Data format 
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 SRX11140411  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140411 (CpG methylation)   Data format 
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 SRX11140412  HMR  Peripheral Blood Mononuclear Cells / SRX11140412 (HMR)   Data format 
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 SRX11140412  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140412 (CpG methylation)   Data format 
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 SRX11140413  HMR  Peripheral Blood Mononuclear Cells / SRX11140413 (HMR)   Data format 
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 SRX11140413  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140413 (CpG methylation)   Data format 
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 SRX11140414  HMR  Peripheral Blood Mononuclear Cells / SRX11140414 (HMR)   Data format 
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 SRX11140414  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140414 (CpG methylation)   Data format 
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 SRX11140415  HMR  Peripheral Blood Mononuclear Cells / SRX11140415 (HMR)   Data format 
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 SRX11140415  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140415 (CpG methylation)   Data format 
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 SRX11140416  HMR  Peripheral Blood Mononuclear Cells / SRX11140416 (HMR)   Data format 
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 SRX11140416  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140416 (CpG methylation)   Data format 
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 SRX11140417  HMR  Peripheral Blood Mononuclear Cells / SRX11140417 (HMR)   Data format 
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 SRX11140417  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140417 (CpG methylation)   Data format 
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 SRX11140418  HMR  Peripheral Blood Mononuclear Cells / SRX11140418 (HMR)   Data format 
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 SRX11140418  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140418 (CpG methylation)   Data format 
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 SRX11140419  HMR  Peripheral Blood Mononuclear Cells / SRX11140419 (HMR)   Data format 
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 SRX11140419  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140419 (CpG methylation)   Data format 
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 SRX11140420  HMR  Peripheral Blood Mononuclear Cells / SRX11140420 (HMR)   Data format 
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 SRX11140420  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140420 (CpG methylation)   Data format 
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 SRX11140421  HMR  Peripheral Blood Mononuclear Cells / SRX11140421 (HMR)   Data format 
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 SRX11140421  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140421 (CpG methylation)   Data format 
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 SRX11140422  HMR  Peripheral Blood Mononuclear Cells / SRX11140422 (HMR)   Data format 
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 SRX11140422  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140422 (CpG methylation)   Data format 
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 SRX11140423  HMR  Peripheral Blood Mononuclear Cells / SRX11140423 (HMR)   Data format 
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 SRX11140423  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140423 (CpG methylation)   Data format 
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 SRX11140424  HMR  Peripheral Blood Mononuclear Cells / SRX11140424 (HMR)   Data format 
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 SRX11140424  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140424 (CpG methylation)   Data format 
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 SRX11140425  HMR  Peripheral Blood Mononuclear Cells / SRX11140425 (HMR)   Data format 
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 SRX11140425  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140425 (CpG methylation)   Data format 
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 SRX11140426  HMR  Peripheral Blood Mononuclear Cells / SRX11140426 (HMR)   Data format 
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 SRX11140426  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140426 (CpG methylation)   Data format 
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 SRX11140427  HMR  Peripheral Blood Mononuclear Cells / SRX11140427 (HMR)   Data format 
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 SRX11140427  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140427 (CpG methylation)   Data format 
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 SRX11140428  HMR  Peripheral Blood Mononuclear Cells / SRX11140428 (HMR)   Data format 
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 SRX11140428  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140428 (CpG methylation)   Data format 
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 SRX11140429  HMR  Peripheral Blood Mononuclear Cells / SRX11140429 (HMR)   Data format 
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 SRX11140429  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140429 (CpG methylation)   Data format 
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 SRX11140430  HMR  Peripheral Blood Mononuclear Cells / SRX11140430 (HMR)   Data format 
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 SRX11140430  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140430 (CpG methylation)   Data format 
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 SRX11140431  HMR  Peripheral Blood Mononuclear Cells / SRX11140431 (HMR)   Data format 
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 SRX11140431  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140431 (CpG methylation)   Data format 
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 SRX11140432  HMR  Peripheral Blood Mononuclear Cells / SRX11140432 (HMR)   Data format 
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 SRX11140432  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140432 (CpG methylation)   Data format 
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 SRX11140433  HMR  Peripheral Blood Mononuclear Cells / SRX11140433 (HMR)   Data format 
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 SRX11140433  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140433 (CpG methylation)   Data format 
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 SRX11140434  HMR  Peripheral Blood Mononuclear Cells / SRX11140434 (HMR)   Data format 
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 SRX11140434  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140434 (CpG methylation)   Data format 
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 SRX11140435  HMR  Peripheral Blood Mononuclear Cells / SRX11140435 (HMR)   Data format 
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 SRX11140435  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140435 (CpG methylation)   Data format 
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 SRX11140436  HMR  Peripheral Blood Mononuclear Cells / SRX11140436 (HMR)   Data format 
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 SRX11140436  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140436 (CpG methylation)   Data format 
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 SRX11140437  HMR  Peripheral Blood Mononuclear Cells / SRX11140437 (HMR)   Data format 
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 SRX11140437  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140437 (CpG methylation)   Data format 
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 SRX11140438  HMR  Peripheral Blood Mononuclear Cells / SRX11140438 (HMR)   Data format 
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 SRX11140438  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140438 (CpG methylation)   Data format 
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 SRX11140439  HMR  Peripheral Blood Mononuclear Cells / SRX11140439 (HMR)   Data format 
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 SRX11140439  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140439 (CpG methylation)   Data format 
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 SRX11140440  HMR  Peripheral Blood Mononuclear Cells / SRX11140440 (HMR)   Data format 
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 SRX11140440  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140440 (CpG methylation)   Data format 
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 SRX11140441  HMR  Peripheral Blood Mononuclear Cells / SRX11140441 (HMR)   Data format 
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 SRX11140441  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140441 (CpG methylation)   Data format 
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 SRX11140442  HMR  Peripheral Blood Mononuclear Cells / SRX11140442 (HMR)   Data format 
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 SRX11140442  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140442 (CpG methylation)   Data format 
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 SRX11140443  HMR  Peripheral Blood Mononuclear Cells / SRX11140443 (HMR)   Data format 
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 SRX11140443  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140443 (CpG methylation)   Data format 
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 SRX11140444  HMR  Peripheral Blood Mononuclear Cells / SRX11140444 (HMR)   Data format 
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 SRX11140444  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140444 (CpG methylation)   Data format 
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 SRX11140445  HMR  Peripheral Blood Mononuclear Cells / SRX11140445 (HMR)   Data format 
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 SRX11140445  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140445 (CpG methylation)   Data format 
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 SRX11140446  HMR  Peripheral Blood Mononuclear Cells / SRX11140446 (HMR)   Data format 
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 SRX11140446  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140446 (CpG methylation)   Data format 
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 SRX11140447  HMR  Peripheral Blood Mononuclear Cells / SRX11140447 (HMR)   Data format 
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 SRX11140447  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140447 (CpG methylation)   Data format 
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 SRX11140448  HMR  Peripheral Blood Mononuclear Cells / SRX11140448 (HMR)   Data format 
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 SRX11140448  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140448 (CpG methylation)   Data format 
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 SRX11140449  HMR  Peripheral Blood Mononuclear Cells / SRX11140449 (HMR)   Data format 
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 SRX11140449  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140449 (CpG methylation)   Data format 
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 SRX11140450  HMR  Peripheral Blood Mononuclear Cells / SRX11140450 (HMR)   Data format 
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 SRX11140450  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140450 (CpG methylation)   Data format 
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 SRX11140451  HMR  Peripheral Blood Mononuclear Cells / SRX11140451 (HMR)   Data format 
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 SRX11140451  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140451 (CpG methylation)   Data format 
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 SRX11140452  HMR  Peripheral Blood Mononuclear Cells / SRX11140452 (HMR)   Data format 
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 SRX11140452  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140452 (CpG methylation)   Data format 
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 SRX11140453  HMR  Peripheral Blood Mononuclear Cells / SRX11140453 (HMR)   Data format 
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 SRX11140453  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140453 (CpG methylation)   Data format 
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 SRX11140454  HMR  Peripheral Blood Mononuclear Cells / SRX11140454 (HMR)   Data format 
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 SRX11140454  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140454 (CpG methylation)   Data format 
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 SRX11140455  HMR  Peripheral Blood Mononuclear Cells / SRX11140455 (HMR)   Data format 
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 SRX11140455  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140455 (CpG methylation)   Data format 
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 SRX11140456  HMR  Peripheral Blood Mononuclear Cells / SRX11140456 (HMR)   Data format 
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 SRX11140456  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140456 (CpG methylation)   Data format 
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 SRX11140457  HMR  Peripheral Blood Mononuclear Cells / SRX11140457 (HMR)   Data format 
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 SRX11140457  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140457 (CpG methylation)   Data format 
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 SRX11140458  HMR  Peripheral Blood Mononuclear Cells / SRX11140458 (HMR)   Data format 
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 SRX11140458  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140458 (CpG methylation)   Data format 
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 SRX11140459  HMR  Peripheral Blood Mononuclear Cells / SRX11140459 (HMR)   Data format 
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 SRX11140459  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140459 (CpG methylation)   Data format 
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 SRX11140460  HMR  Peripheral Blood Mononuclear Cells / SRX11140460 (HMR)   Data format 
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 SRX11140460  CpG methylation  Peripheral Blood Mononuclear Cells / SRX11140460 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: Identification of DNA Methylation Predicts Onset of PTSD and Depression Following Trauma
SRA: SRP323990
GEO: GSE178128
Pubmed: 34630024

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX11140395 Peripheral Blood Mononuclear Cells 0.800 46.4 80133 943.9 1182 890.1 4176 13518.7 0.986 GSM5380636: PTSD008-D1; Homo sapiens; Bisulfite-Seq
SRX11140396 Peripheral Blood Mononuclear Cells 0.799 43.1 80321 936.9 1171 889.1 4028 13688.2 0.984 GSM5380637: PTSD008-D2; Homo sapiens; Bisulfite-Seq
SRX11140397 Peripheral Blood Mononuclear Cells 0.797 45.7 79105 952.8 1411 889.8 4341 12116.7 0.984 GSM5380638: PTSD009-D1; Homo sapiens; Bisulfite-Seq
SRX11140398 Peripheral Blood Mononuclear Cells 0.797 50.2 78160 966.2 1441 878.1 3854 12784.6 0.985 GSM5380639: PTSD009-D2; Homo sapiens; Bisulfite-Seq
SRX11140399 Peripheral Blood Mononuclear Cells 0.801 45.4 83742 900.8 1121 882.5 4533 11648.3 0.985 GSM5380640: PTSD018-D1; Homo sapiens; Bisulfite-Seq
SRX11140400 Peripheral Blood Mononuclear Cells 0.794 58.8 80741 927.2 1116 884.6 3589 12750.7 0.985 GSM5380641: PTSD018-D2; Homo sapiens; Bisulfite-Seq
SRX11140401 Peripheral Blood Mononuclear Cells 0.801 49.2 73020 1057.8 2342 991.9 3691 16783.0 0.985 GSM5380642: PTSD019-D1; Homo sapiens; Bisulfite-Seq
SRX11140402 Peripheral Blood Mononuclear Cells 0.798 57.1 71611 1079.9 2578 980.4 4257 15703.9 0.985 GSM5380643: PTSD019-D2; Homo sapiens; Bisulfite-Seq
SRX11140403 Peripheral Blood Mononuclear Cells 0.795 50.2 74281 966.2 1674 855.7 3784 12211.5 0.985 GSM5380644: PTSD021-D1; Homo sapiens; Bisulfite-Seq
SRX11140404 Peripheral Blood Mononuclear Cells 0.793 52.4 77894 946.2 1278 878.4 4003 12352.2 0.985 GSM5380645: PTSD021-D2; Homo sapiens; Bisulfite-Seq
SRX11140405 Peripheral Blood Mononuclear Cells 0.795 51.1 77650 986.9 1729 1017.1 3816 15218.8 0.985 GSM5380646: PTSD024-D1; Homo sapiens; Bisulfite-Seq
SRX11140406 Peripheral Blood Mononuclear Cells 0.791 54.2 79965 971.3 1672 1019.2 3962 15238.0 0.985 GSM5380647: PTSD024-D2; Homo sapiens; Bisulfite-Seq
SRX11140407 Peripheral Blood Mononuclear Cells 0.798 47.8 81573 954.9 1659 1057.6 3860 14091.4 0.985 GSM5380648: PTSD026-D1; Homo sapiens; Bisulfite-Seq
SRX11140408 Peripheral Blood Mononuclear Cells 0.796 46.6 82940 940.0 1589 1049.5 3966 13840.1 0.985 GSM5380649: PTSD026-D2; Homo sapiens; Bisulfite-Seq
SRX11140409 Peripheral Blood Mononuclear Cells 0.802 55.0 81849 932.0 1727 1080.8 4315 11294.6 0.986 GSM5380650: PTSD028-D1; Homo sapiens; Bisulfite-Seq
SRX11140410 Peripheral Blood Mononuclear Cells 0.797 56.0 73906 1001.8 2319 987.9 3852 11787.9 0.985 GSM5380651: PTSD028-D2; Homo sapiens; Bisulfite-Seq
SRX11140411 Peripheral Blood Mononuclear Cells 0.794 43.3 84463 912.4 1146 901.8 4063 13252.3 0.985 GSM5380652: PTSD029-D1; Homo sapiens; Bisulfite-Seq
SRX11140412 Peripheral Blood Mononuclear Cells 0.793 50.5 86724 910.9 1143 887.8 3955 13961.4 0.985 GSM5380653: PTSD029-D2; Homo sapiens; Bisulfite-Seq
SRX11140413 Peripheral Blood Mononuclear Cells 0.799 45.2 85104 890.4 1138 903.1 4279 11191.9 0.985 GSM5380654: PTSD038-D1; Homo sapiens; Bisulfite-Seq
SRX11140414 Peripheral Blood Mononuclear Cells 0.790 47.3 82084 914.4 1169 881.7 4088 11275.6 0.986 GSM5380655: PTSD038-D2; Homo sapiens; Bisulfite-Seq
SRX11140415 Peripheral Blood Mononuclear Cells 0.801 45.2 82044 933.2 1175 886.4 4595 12365.9 0.985 GSM5380656: PTSD047-D1; Homo sapiens; Bisulfite-Seq
SRX11140416 Peripheral Blood Mononuclear Cells 0.796 45.9 74792 971.6 1534 858.9 4575 11418.8 0.985 GSM5380657: PTSD047-D2; Homo sapiens; Bisulfite-Seq
SRX11140417 Peripheral Blood Mononuclear Cells 0.798 46.4 83172 911.0 1125 898.1 4018 12816.5 0.985 GSM5380658: PTSD053-D1; Homo sapiens; Bisulfite-Seq
SRX11140418 Peripheral Blood Mononuclear Cells 0.792 50.4 81821 928.4 1103 895.4 3881 13137.7 0.985 GSM5380659: PTSD053-D2; Homo sapiens; Bisulfite-Seq
SRX11140419 Peripheral Blood Mononuclear Cells 0.800 43.7 80163 952.6 1588 1059.7 4101 14336.9 0.985 GSM5380660: PTSD054-D1; Homo sapiens; Bisulfite-Seq
SRX11140420 Peripheral Blood Mononuclear Cells 0.795 57.4 78534 987.4 1695 1028.6 4123 14623.3 0.985 GSM5380661: PTSD054-D2; Homo sapiens; Bisulfite-Seq
SRX11140421 Peripheral Blood Mononuclear Cells 0.802 52.8 77773 963.8 1417 868.6 4186 12074.4 0.985 GSM5380662: PTSD057-D1; Homo sapiens; Bisulfite-Seq
SRX11140422 Peripheral Blood Mononuclear Cells 0.795 60.2 80604 954.5 1230 876.0 4212 12474.8 0.985 GSM5380663: PTSD057-D2; Homo sapiens; Bisulfite-Seq
SRX11140423 Peripheral Blood Mononuclear Cells 0.799 50.9 76483 991.3 1925 1014.3 3793 12910.5 0.984 GSM5380664: PTSD059-D1; Homo sapiens; Bisulfite-Seq
SRX11140424 Peripheral Blood Mononuclear Cells 0.792 52.1 81067 957.2 1673 1045.3 4274 12632.1 0.985 GSM5380665: PTSD059-D2; Homo sapiens; Bisulfite-Seq
SRX11140425 Peripheral Blood Mononuclear Cells 0.796 46.2 77246 950.6 1335 870.3 4147 11228.2 0.985 GSM5380666: PTSD063-D1; Homo sapiens; Bisulfite-Seq
SRX11140426 Peripheral Blood Mononuclear Cells 0.795 43.6 83238 906.7 1070 888.3 4241 11717.9 0.985 GSM5380667: PTSD063-D2; Homo sapiens; Bisulfite-Seq
SRX11140427 Peripheral Blood Mononuclear Cells 0.768 45.1 66866 981.2 8296 974.6 2988 12036.9 0.985 GSM5380668: PTSD071-D1; Homo sapiens; Bisulfite-Seq
SRX11140428 Peripheral Blood Mononuclear Cells 0.761 43.2 64482 1005.9 9348 1010.9 3018 11549.8 0.985 GSM5380669: PTSD071-D2; Homo sapiens; Bisulfite-Seq
SRX11140429 Peripheral Blood Mononuclear Cells 0.789 46.0 81471 967.0 1696 1090.3 3928 14871.8 0.985 GSM5380670: PTSD079-D1; Homo sapiens; Bisulfite-Seq
SRX11140430 Peripheral Blood Mononuclear Cells 0.782 43.0 76663 994.9 1841 1046.7 4054 13946.0 0.985 GSM5380671: PTSD079-D2; Homo sapiens; Bisulfite-Seq
SRX11140431 Peripheral Blood Mononuclear Cells 0.796 48.6 72886 1000.5 2252 983.2 3626 12413.7 0.984 GSM5380672: PTSD100-D1; Homo sapiens; Bisulfite-Seq
SRX11140432 Peripheral Blood Mononuclear Cells 0.791 46.4 74007 994.0 2033 996.4 3866 12444.8 0.985 GSM5380673: PTSD100-D2; Homo sapiens; Bisulfite-Seq
SRX11140433 Peripheral Blood Mononuclear Cells 0.796 43.2 78347 971.2 1271 877.5 4273 13551.2 0.985 GSM5380674: PTSD102-D1; Homo sapiens; Bisulfite-Seq
SRX11140434 Peripheral Blood Mononuclear Cells 0.791 46.3 82233 950.8 1110 879.6 3937 14651.0 0.985 GSM5380675: PTSD102-D2; Homo sapiens; Bisulfite-Seq
SRX11140435 Peripheral Blood Mononuclear Cells 0.794 45.8 83535 911.2 1136 885.1 3902 12819.5 0.985 GSM5380676: PTSD108-D1; Homo sapiens; Bisulfite-Seq
SRX11140436 Peripheral Blood Mononuclear Cells 0.792 58.0 81664 940.4 1194 875.0 4203 12419.2 0.985 GSM5380677: PTSD108-D2; Homo sapiens; Bisulfite-Seq
SRX11140437 Peripheral Blood Mononuclear Cells 0.785 59.2 82330 974.5 1270 906.7 3936 14629.6 0.985 GSM5380678: PTSD111-D1; Homo sapiens; Bisulfite-Seq
SRX11140438 Peripheral Blood Mononuclear Cells 0.786 60.1 87542 937.8 1214 899.8 4099 14676.8 0.985 GSM5380679: PTSD111-D2; Homo sapiens; Bisulfite-Seq
SRX11140439 Peripheral Blood Mononuclear Cells 0.790 54.6 78659 960.7 1827 1059.1 3896 12461.1 0.985 GSM5380680: PTSD112-D1; Homo sapiens; Bisulfite-Seq
SRX11140440 Peripheral Blood Mononuclear Cells 0.784 50.0 72237 989.9 2520 985.1 3938 11455.1 0.985 GSM5380681: PTSD112-D2; Homo sapiens; Bisulfite-Seq
SRX11140441 Peripheral Blood Mononuclear Cells 0.799 51.3 84434 898.2 1124 894.0 4783 10785.0 0.985 GSM5380682: PTSD114-D1; Homo sapiens; Bisulfite-Seq
SRX11140442 Peripheral Blood Mononuclear Cells 0.799 43.3 83843 889.9 1120 895.2 4194 11295.7 0.985 GSM5380683: PTSD114-D2; Homo sapiens; Bisulfite-Seq
SRX11140443 Peripheral Blood Mononuclear Cells 0.795 43.6 72824 1026.2 2276 969.3 3796 15792.7 0.985 GSM5380684: PTSD116-D1; Homo sapiens; Bisulfite-Seq
SRX11140444 Peripheral Blood Mononuclear Cells 0.795 47.5 76745 1007.5 1739 1018.3 4191 15738.0 0.985 GSM5380685: PTSD116-D2; Homo sapiens; Bisulfite-Seq
SRX11140445 Peripheral Blood Mononuclear Cells 0.797 44.0 84248 932.3 1052 879.5 4369 14733.7 0.985 GSM5380686: PTSD124-D1; Homo sapiens; Bisulfite-Seq
SRX11140446 Peripheral Blood Mononuclear Cells 0.799 50.8 84228 954.6 1073 922.2 3998 16006.0 0.985 GSM5380687: PTSD124-D2; Homo sapiens; Bisulfite-Seq
SRX11140447 Peripheral Blood Mononuclear Cells 0.799 50.8 86137 930.0 1083 934.1 4627 13776.7 0.985 GSM5380688: PTSD127-D1; Homo sapiens; Bisulfite-Seq
SRX11140448 Peripheral Blood Mononuclear Cells 0.796 43.7 85567 918.7 1003 878.0 4140 14581.2 0.985 GSM5380689: PTSD127-D2; Homo sapiens; Bisulfite-Seq
SRX11140449 Peripheral Blood Mononuclear Cells 0.802 22.8 66828 998.8 2285 970.1 4053 10717.0 0.984 GSM5380690: PTSD136-D1; Homo sapiens; Bisulfite-Seq
SRX11140450 Peripheral Blood Mononuclear Cells 0.805 52.6 70429 1057.8 2763 988.4 3890 12601.1 0.985 GSM5380691: PTSD136-D2; Homo sapiens; Bisulfite-Seq
SRX11140451 Peripheral Blood Mononuclear Cells 0.802 48.2 84767 928.0 1116 902.7 4368 13515.5 0.984 GSM5380692: PTSD138-D1; Homo sapiens; Bisulfite-Seq
SRX11140452 Peripheral Blood Mononuclear Cells 0.801 67.8 82979 981.0 1185 896.7 4345 13790.1 0.985 GSM5380693: PTSD138-D2; Homo sapiens; Bisulfite-Seq
SRX11140453 Peripheral Blood Mononuclear Cells 0.798 55.2 84526 901.1 1103 896.0 4612 11147.7 0.985 GSM5380694: PTSD153-D1; Homo sapiens; Bisulfite-Seq
SRX11140454 Peripheral Blood Mononuclear Cells 0.796 55.1 81543 921.5 1145 877.1 4410 11125.5 0.985 GSM5380695: PTSD153-D2; Homo sapiens; Bisulfite-Seq
SRX11140455 Peripheral Blood Mononuclear Cells 0.801 56.2 73473 988.9 1867 865.0 4324 11836.0 0.984 GSM5380696: PTSD165-D1; Homo sapiens; Bisulfite-Seq
SRX11140456 Peripheral Blood Mononuclear Cells 0.800 39.6 74232 958.3 1341 863.2 4264 11734.8 0.985 GSM5380697: PTSD165-D2; Homo sapiens; Bisulfite-Seq
SRX11140457 Peripheral Blood Mononuclear Cells 0.798 44.9 83999 907.8 1128 897.8 4421 12430.5 0.985 GSM5380698: PTSD172-D1; Homo sapiens; Bisulfite-Seq
SRX11140458 Peripheral Blood Mononuclear Cells 0.802 43.1 73043 1001.8 2243 862.9 4572 11778.0 0.985 GSM5380699: PTSD172-D2; Homo sapiens; Bisulfite-Seq
SRX11140459 Peripheral Blood Mononuclear Cells 0.797 40.4 79669 931.6 1526 1007.9 3867 13880.8 0.985 GSM5380700: PTSD179-D1; Homo sapiens; Bisulfite-Seq
SRX11140460 Peripheral Blood Mononuclear Cells 0.795 41.8 77493 950.7 1549 1011.9 3937 13575.2 0.985 GSM5380701: PTSD179-D2; Homo sapiens; Bisulfite-Seq

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.