Human methylome studies SRP101887 Track Settings
 
Neuronal brain region-specific DNA methylation and chromatin accessibility are associated with neuropsychiatric trait heritability [Bisulfite-Seq] [Bulk Tissue, NeuN Neg, NeuN Pos]

Track collection: Human methylome studies

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experiment
SRX2640456 
SRX2640457 
SRX2640458 
SRX2640459 
SRX2640460 
SRX2640461 
SRX2640462 
SRX2640463 
SRX2640464 
SRX2640465 
SRX2640466 
SRX2640467 
SRX2640468 
SRX2640469 
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SRX2640471 
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SRX2640473 
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SRX2640475 
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SRX2640477 
SRX2640478 
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SRX2640480 
SRX2640481 
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SRX2640483 
SRX2640484 
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SRX2640486 
SRX2640487 
SRX2640488 
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SRX2640526 
SRX2640527 
experiment
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 SRX2640456  HMR  NeuN Neg / SRX2640456 (HMR)   Data format 
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 SRX2640456  CpG methylation  NeuN Neg / SRX2640456 (CpG methylation)   Data format 
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 SRX2640457  HMR  NeuN Pos / SRX2640457 (HMR)   Data format 
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 SRX2640457  CpG methylation  NeuN Pos / SRX2640457 (CpG methylation)   Data format 
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 SRX2640458  HMR  NeuN Neg / SRX2640458 (HMR)   Data format 
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 SRX2640458  CpG methylation  NeuN Neg / SRX2640458 (CpG methylation)   Data format 
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 SRX2640459  HMR  NeuN Pos / SRX2640459 (HMR)   Data format 
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 SRX2640459  CpG methylation  NeuN Pos / SRX2640459 (CpG methylation)   Data format 
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 SRX2640460  HMR  NeuN Neg / SRX2640460 (HMR)   Data format 
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 SRX2640460  CpG methylation  NeuN Neg / SRX2640460 (CpG methylation)   Data format 
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 SRX2640461  HMR  NeuN Pos / SRX2640461 (HMR)   Data format 
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 SRX2640461  CpG methylation  NeuN Pos / SRX2640461 (CpG methylation)   Data format 
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 SRX2640462  HMR  NeuN Neg / SRX2640462 (HMR)   Data format 
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 SRX2640462  CpG methylation  NeuN Neg / SRX2640462 (CpG methylation)   Data format 
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 SRX2640463  HMR  NeuN Pos / SRX2640463 (HMR)   Data format 
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 SRX2640463  CpG methylation  NeuN Pos / SRX2640463 (CpG methylation)   Data format 
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 SRX2640464  HMR  NeuN Pos / SRX2640464 (HMR)   Data format 
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 SRX2640464  CpG methylation  NeuN Pos / SRX2640464 (CpG methylation)   Data format 
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 SRX2640465  HMR  NeuN Neg / SRX2640465 (HMR)   Data format 
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 SRX2640465  CpG methylation  NeuN Neg / SRX2640465 (CpG methylation)   Data format 
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 SRX2640466  HMR  NeuN Pos / SRX2640466 (HMR)   Data format 
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 SRX2640466  CpG methylation  NeuN Pos / SRX2640466 (CpG methylation)   Data format 
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 SRX2640467  HMR  NeuN Neg / SRX2640467 (HMR)   Data format 
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 SRX2640467  CpG methylation  NeuN Neg / SRX2640467 (CpG methylation)   Data format 
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 SRX2640468  HMR  NeuN Pos / SRX2640468 (HMR)   Data format 
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 SRX2640468  CpG methylation  NeuN Pos / SRX2640468 (CpG methylation)   Data format 
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 SRX2640469  HMR  NeuN Neg / SRX2640469 (HMR)   Data format 
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 SRX2640469  CpG methylation  NeuN Neg / SRX2640469 (CpG methylation)   Data format 
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 SRX2640470  HMR  NeuN Pos / SRX2640470 (HMR)   Data format 
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 SRX2640470  CpG methylation  NeuN Pos / SRX2640470 (CpG methylation)   Data format 
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 SRX2640471  HMR  NeuN Neg / SRX2640471 (HMR)   Data format 
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 SRX2640471  CpG methylation  NeuN Neg / SRX2640471 (CpG methylation)   Data format 
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 SRX2640472  HMR  NeuN Neg / SRX2640472 (HMR)   Data format 
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 SRX2640472  CpG methylation  NeuN Neg / SRX2640472 (CpG methylation)   Data format 
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 SRX2640473  HMR  NeuN Pos / SRX2640473 (HMR)   Data format 
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 SRX2640473  CpG methylation  NeuN Pos / SRX2640473 (CpG methylation)   Data format 
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 SRX2640474  HMR  NeuN Neg / SRX2640474 (HMR)   Data format 
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 SRX2640474  CpG methylation  NeuN Neg / SRX2640474 (CpG methylation)   Data format 
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 SRX2640475  HMR  NeuN Pos / SRX2640475 (HMR)   Data format 
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 SRX2640475  CpG methylation  NeuN Pos / SRX2640475 (CpG methylation)   Data format 
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 SRX2640476  HMR  NeuN Neg / SRX2640476 (HMR)   Data format 
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 SRX2640476  CpG methylation  NeuN Neg / SRX2640476 (CpG methylation)   Data format 
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 SRX2640477  HMR  NeuN Pos / SRX2640477 (HMR)   Data format 
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 SRX2640477  CpG methylation  NeuN Pos / SRX2640477 (CpG methylation)   Data format 
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 SRX2640478  HMR  NeuN Neg / SRX2640478 (HMR)   Data format 
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 SRX2640478  CpG methylation  NeuN Neg / SRX2640478 (CpG methylation)   Data format 
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 SRX2640479  HMR  NeuN Pos / SRX2640479 (HMR)   Data format 
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 SRX2640479  CpG methylation  NeuN Pos / SRX2640479 (CpG methylation)   Data format 
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 SRX2640480  HMR  NeuN Neg / SRX2640480 (HMR)   Data format 
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 SRX2640480  CpG methylation  NeuN Neg / SRX2640480 (CpG methylation)   Data format 
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 SRX2640481  HMR  NeuN Pos / SRX2640481 (HMR)   Data format 
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 SRX2640481  CpG methylation  NeuN Pos / SRX2640481 (CpG methylation)   Data format 
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 SRX2640482  HMR  NeuN Neg / SRX2640482 (HMR)   Data format 
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 SRX2640482  CpG methylation  NeuN Neg / SRX2640482 (CpG methylation)   Data format 
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 SRX2640483  HMR  NeuN Pos / SRX2640483 (HMR)   Data format 
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 SRX2640483  CpG methylation  NeuN Pos / SRX2640483 (CpG methylation)   Data format 
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 SRX2640484  HMR  NeuN Neg / SRX2640484 (HMR)   Data format 
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 SRX2640484  CpG methylation  NeuN Neg / SRX2640484 (CpG methylation)   Data format 
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 SRX2640485  HMR  NeuN Pos / SRX2640485 (HMR)   Data format 
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 SRX2640485  CpG methylation  NeuN Pos / SRX2640485 (CpG methylation)   Data format 
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 SRX2640486  HMR  NeuN Pos / SRX2640486 (HMR)   Data format 
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 SRX2640486  CpG methylation  NeuN Pos / SRX2640486 (CpG methylation)   Data format 
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 SRX2640487  HMR  NeuN Neg / SRX2640487 (HMR)   Data format 
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 SRX2640487  CpG methylation  NeuN Neg / SRX2640487 (CpG methylation)   Data format 
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 SRX2640488  HMR  NeuN Pos / SRX2640488 (HMR)   Data format 
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 SRX2640488  CpG methylation  NeuN Pos / SRX2640488 (CpG methylation)   Data format 
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 SRX2640489  HMR  NeuN Neg / SRX2640489 (HMR)   Data format 
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 SRX2640489  CpG methylation  NeuN Neg / SRX2640489 (CpG methylation)   Data format 
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 SRX2640490  HMR  NeuN Pos / SRX2640490 (HMR)   Data format 
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 SRX2640490  CpG methylation  NeuN Pos / SRX2640490 (CpG methylation)   Data format 
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 SRX2640491  HMR  NeuN Neg / SRX2640491 (HMR)   Data format 
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 SRX2640491  CpG methylation  NeuN Neg / SRX2640491 (CpG methylation)   Data format 
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 SRX2640492  HMR  NeuN Pos / SRX2640492 (HMR)   Data format 
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 SRX2640492  CpG methylation  NeuN Pos / SRX2640492 (CpG methylation)   Data format 
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 SRX2640493  HMR  NeuN Neg / SRX2640493 (HMR)   Data format 
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 SRX2640493  CpG methylation  NeuN Neg / SRX2640493 (CpG methylation)   Data format 
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 SRX2640494  HMR  NeuN Pos / SRX2640494 (HMR)   Data format 
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 SRX2640494  CpG methylation  NeuN Pos / SRX2640494 (CpG methylation)   Data format 
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 SRX2640495  HMR  NeuN Neg / SRX2640495 (HMR)   Data format 
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 SRX2640495  CpG methylation  NeuN Neg / SRX2640495 (CpG methylation)   Data format 
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 SRX2640496  HMR  NeuN Pos / SRX2640496 (HMR)   Data format 
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 SRX2640496  CpG methylation  NeuN Pos / SRX2640496 (CpG methylation)   Data format 
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 SRX2640497  HMR  NeuN Neg / SRX2640497 (HMR)   Data format 
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 SRX2640497  CpG methylation  NeuN Neg / SRX2640497 (CpG methylation)   Data format 
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 SRX2640498  HMR  NeuN Pos / SRX2640498 (HMR)   Data format 
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 SRX2640498  CpG methylation  NeuN Pos / SRX2640498 (CpG methylation)   Data format 
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 SRX2640499  HMR  NeuN Neg / SRX2640499 (HMR)   Data format 
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 SRX2640499  CpG methylation  NeuN Neg / SRX2640499 (CpG methylation)   Data format 
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 SRX2640500  HMR  NeuN Pos / SRX2640500 (HMR)   Data format 
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 SRX2640500  CpG methylation  NeuN Pos / SRX2640500 (CpG methylation)   Data format 
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 SRX2640501  HMR  Bulk Tissue / SRX2640501 (HMR)   Data format 
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 SRX2640501  CpG methylation  Bulk Tissue / SRX2640501 (CpG methylation)   Data format 
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 SRX2640502  HMR  Bulk Tissue / SRX2640502 (HMR)   Data format 
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 SRX2640502  CpG methylation  Bulk Tissue / SRX2640502 (CpG methylation)   Data format 
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 SRX2640503  HMR  Bulk Tissue / SRX2640503 (HMR)   Data format 
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 SRX2640503  CpG methylation  Bulk Tissue / SRX2640503 (CpG methylation)   Data format 
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 SRX2640504  HMR  Bulk Tissue / SRX2640504 (HMR)   Data format 
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 SRX2640504  CpG methylation  Bulk Tissue / SRX2640504 (CpG methylation)   Data format 
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 SRX2640505  HMR  Bulk Tissue / SRX2640505 (HMR)   Data format 
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 SRX2640505  CpG methylation  Bulk Tissue / SRX2640505 (CpG methylation)   Data format 
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 SRX2640506  HMR  Bulk Tissue / SRX2640506 (HMR)   Data format 
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 SRX2640506  CpG methylation  Bulk Tissue / SRX2640506 (CpG methylation)   Data format 
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 SRX2640507  HMR  Bulk Tissue / SRX2640507 (HMR)   Data format 
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 SRX2640507  CpG methylation  Bulk Tissue / SRX2640507 (CpG methylation)   Data format 
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 SRX2640508  HMR  Bulk Tissue / SRX2640508 (HMR)   Data format 
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 SRX2640508  CpG methylation  Bulk Tissue / SRX2640508 (CpG methylation)   Data format 
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 SRX2640509  HMR  Bulk Tissue / SRX2640509 (HMR)   Data format 
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 SRX2640509  CpG methylation  Bulk Tissue / SRX2640509 (CpG methylation)   Data format 
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 SRX2640510  HMR  Bulk Tissue / SRX2640510 (HMR)   Data format 
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 SRX2640510  CpG methylation  Bulk Tissue / SRX2640510 (CpG methylation)   Data format 
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 SRX2640511  HMR  Bulk Tissue / SRX2640511 (HMR)   Data format 
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 SRX2640511  CpG methylation  Bulk Tissue / SRX2640511 (CpG methylation)   Data format 
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 SRX2640512  HMR  Bulk Tissue / SRX2640512 (HMR)   Data format 
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 SRX2640512  CpG methylation  Bulk Tissue / SRX2640512 (CpG methylation)   Data format 
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 SRX2640513  HMR  Bulk Tissue / SRX2640513 (HMR)   Data format 
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 SRX2640513  CpG methylation  Bulk Tissue / SRX2640513 (CpG methylation)   Data format 
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 SRX2640514  HMR  Bulk Tissue / SRX2640514 (HMR)   Data format 
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 SRX2640514  CpG methylation  Bulk Tissue / SRX2640514 (CpG methylation)   Data format 
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 SRX2640515  HMR  Bulk Tissue / SRX2640515 (HMR)   Data format 
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 SRX2640515  CpG methylation  Bulk Tissue / SRX2640515 (CpG methylation)   Data format 
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 SRX2640516  HMR  Bulk Tissue / SRX2640516 (HMR)   Data format 
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 SRX2640516  CpG methylation  Bulk Tissue / SRX2640516 (CpG methylation)   Data format 
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 SRX2640517  HMR  Bulk Tissue / SRX2640517 (HMR)   Data format 
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 SRX2640517  CpG methylation  Bulk Tissue / SRX2640517 (CpG methylation)   Data format 
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 SRX2640518  HMR  Bulk Tissue / SRX2640518 (HMR)   Data format 
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 SRX2640518  CpG methylation  Bulk Tissue / SRX2640518 (CpG methylation)   Data format 
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 SRX2640519  HMR  Bulk Tissue / SRX2640519 (HMR)   Data format 
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 SRX2640519  CpG methylation  Bulk Tissue / SRX2640519 (CpG methylation)   Data format 
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 SRX2640520  HMR  Bulk Tissue / SRX2640520 (HMR)   Data format 
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 SRX2640520  CpG methylation  Bulk Tissue / SRX2640520 (CpG methylation)   Data format 
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 SRX2640521  HMR  Bulk Tissue / SRX2640521 (HMR)   Data format 
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 SRX2640521  CpG methylation  Bulk Tissue / SRX2640521 (CpG methylation)   Data format 
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 SRX2640522  HMR  Bulk Tissue / SRX2640522 (HMR)   Data format 
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 SRX2640522  CpG methylation  Bulk Tissue / SRX2640522 (CpG methylation)   Data format 
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 SRX2640523  HMR  Bulk Tissue / SRX2640523 (HMR)   Data format 
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 SRX2640523  CpG methylation  Bulk Tissue / SRX2640523 (CpG methylation)   Data format 
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 SRX2640524  HMR  Bulk Tissue / SRX2640524 (HMR)   Data format 
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 SRX2640524  CpG methylation  Bulk Tissue / SRX2640524 (CpG methylation)   Data format 
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 SRX2640525  HMR  Bulk Tissue / SRX2640525 (HMR)   Data format 
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 SRX2640525  CpG methylation  Bulk Tissue / SRX2640525 (CpG methylation)   Data format 
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 SRX2640526  HMR  Bulk Tissue / SRX2640526 (HMR)   Data format 
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 SRX2640526  CpG methylation  Bulk Tissue / SRX2640526 (CpG methylation)   Data format 
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 SRX2640527  HMR  Bulk Tissue / SRX2640527 (HMR)   Data format 
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 SRX2640527  CpG methylation  Bulk Tissue / SRX2640527 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: Neuronal brain region-specific DNA methylation and chromatin accessibility are associated with neuropsychiatric trait heritability [Bisulfite-Seq]
SRA: SRP101887
GEO: GSE96612
Pubmed: 30643296

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX2640456 NeuN Neg 0.788 10.8 57846 1167.0 227 968.5 3474 23785.6 0.992 GSM2536541: 5248_BA24_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640457 NeuN Pos 0.812 12.1 49306 1325.6 2372 872.9 4409 22136.3 0.952 GSM2536542: 5248_BA24_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640458 NeuN Neg 0.785 9.8 58589 1181.5 109 1117.0 3367 23764.1 0.991 GSM2536543: 5248_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640459 NeuN Pos 0.810 9.0 54787 1417.7 958 879.3 5316 21491.7 0.949 GSM2536544: 5248_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640460 NeuN Neg 0.787 10.3 56804 1183.5 285 926.4 3567 23019.2 0.992 GSM2536545: 5248_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640461 NeuN Pos 0.800 10.9 53815 1330.5 1393 844.4 4702 22058.8 0.957 GSM2536546: 5248_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640462 NeuN Neg 0.783 13.4 67634 1144.0 149 1098.2 3751 24289.4 0.993 GSM2536547: 5248_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640463 NeuN Pos 0.820 14.5 65706 1234.0 708 854.6 5143 22421.6 0.951 GSM2536548: 5248_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640464 NeuN Pos 0.824 19.7 56547 1362.2 4987 880.3 4926 22134.8 0.947 GSM2536549: 5284_BA24_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640465 NeuN Neg 0.787 15.3 62687 1154.1 652 879.8 3837 22223.9 0.991 GSM2536550: 5284_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640466 NeuN Pos 0.818 16.6 63275 1413.5 3582 873.4 5405 21221.8 0.945 GSM2536551: 5284_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640467 NeuN Neg 0.787 12.0 59592 1156.1 240 914.9 3787 21964.6 0.992 GSM2536552: 5284_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640468 NeuN Pos 0.815 12.4 51156 1311.7 2320 831.6 4616 22105.7 0.943 GSM2536553: 5284_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640469 NeuN Neg 0.788 11.2 59770 1146.3 251 938.1 3538 23761.2 0.992 GSM2536554: 5284_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640470 NeuN Pos 0.826 11.5 45206 1270.4 823 812.5 4029 23293.8 0.955 GSM2536555: 5284_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640471 NeuN Neg 0.789 17.1 64261 1174.1 932 960.1 4558 29143.0 0.990 GSM2536556: 5552_BA24_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640472 NeuN Neg 0.779 15.1 61727 1151.2 590 943.0 4449 28467.3 0.991 GSM2536557: 5552_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640473 NeuN Pos 0.802 15.1 59666 1384.7 3358 900.9 5994 29938.5 0.950 GSM2536558: 5552_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640474 NeuN Neg 0.774 12.7 61378 1126.8 265 1021.0 4464 25158.4 0.992 GSM2536559: 5552_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640475 NeuN Pos 0.798 17.5 55477 1329.8 4789 881.0 5012 37216.9 0.950 GSM2536560: 5552_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640476 NeuN Neg 0.776 13.2 59221 1133.1 323 961.7 4046 26077.8 0.993 GSM2536561: 5552_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640477 NeuN Pos 0.813 11.2 47809 1237.0 673 890.8 4328 42322.8 0.951 GSM2536562: 5552_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640478 NeuN Neg 0.780 11.5 56368 1159.1 224 972.9 3578 22275.9 0.992 GSM2536563: 5569_BA24_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640479 NeuN Pos 0.816 15.5 57094 1341.5 3267 856.8 4762 21124.3 0.945 GSM2536564: 5569_BA24_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640480 NeuN Neg 0.782 10.6 57267 1172.4 146 999.3 3838 21916.4 0.992 GSM2536565: 5569_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640481 NeuN Pos 0.811 11.1 56571 1380.1 1731 857.1 5252 21471.7 0.946 GSM2536566: 5569_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640482 NeuN Neg 0.785 10.0 49047 1197.3 325 919.6 3710 19637.5 0.992 GSM2536567: 5569_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640483 NeuN Pos 0.796 15.6 73212 1445.3 3227 830.3 4951 27064.2 0.953 GSM2536568: 5569_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640484 NeuN Neg 0.786 15.4 62454 1153.7 742 891.2 4063 22335.3 0.993 GSM2536569: 5569_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640485 NeuN Pos 0.822 16.1 55300 1258.9 976 831.2 4109 24273.7 0.959 GSM2536570: 5569_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640486 NeuN Pos 0.818 16.6 56318 1389.7 4084 896.4 4388 49324.2 0.953 GSM2536571: 5570_BA24_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640487 NeuN Neg 0.780 16.3 68067 1164.0 344 1030.0 4445 32647.9 0.992 GSM2536572: 5570_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640488 NeuN Pos 0.802 15.3 64371 1439.0 3152 888.6 5729 35649.5 0.951 GSM2536573: 5570_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640489 NeuN Neg 0.780 14.0 58574 1175.9 542 911.8 4308 27922.7 0.993 GSM2536574: 5570_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640490 NeuN Pos 0.795 13.8 56015 1326.8 2557 844.7 5102 35890.2 0.956 GSM2536575: 5570_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640491 NeuN Neg 0.776 11.2 59430 1194.1 259 1015.4 4479 29432.4 0.992 GSM2536576: 5570_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640492 NeuN Pos 0.818 10.3 47373 1277.0 399 867.0 3364 62661.4 0.962 GSM2536577: 5570_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640493 NeuN Neg 0.766 13.9 55809 1131.7 754 896.1 4175 20912.7 0.992 GSM2536578: 5628_BA24_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640494 NeuN Pos 0.801 12.2 46588 1282.2 2132 884.7 5475 23841.5 0.955 GSM2536579: 5628_BA24_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640495 NeuN Neg 0.762 12.0 59659 1146.7 351 978.9 4111 22828.0 0.986 GSM2536580: 5628_BA9_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640496 NeuN Pos 0.802 10.1 49873 1360.6 1643 903.6 5558 23309.1 0.951 GSM2536581: 5628_BA9_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640497 NeuN Neg 0.760 17.6 66239 1119.8 612 1002.5 4652 21981.1 0.992 GSM2536582: 5628_HC_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640498 NeuN Pos 0.803 15.8 46107 1274.3 3832 873.9 5195 33374.5 0.954 GSM2536583: 5628_HC_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640499 NeuN Neg 0.763 11.3 57432 1130.9 304 944.3 3991 22262.9 0.992 GSM2536584: 5628_NAcc_neg (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640500 NeuN Pos 0.807 10.8 41429 1265.4 626 861.4 4422 39388.3 0.962 GSM2536585: 5628_NAcc_pos (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640501 Bulk Tissue 0.747 11.0 45249 1238.6 874 926.1 4062 27978.9 0.987 GSM2536586: 5085_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640502 Bulk Tissue 0.736 11.7 37871 1233.5 1448 863.6 3808 19391.3 0.980 GSM2536587: 5086_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640503 Bulk Tissue 0.728 11.6 44743 1224.9 983 901.9 3548 23480.3 0.986 GSM2536588: 5086_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640504 Bulk Tissue 0.743 11.4 40964 1213.2 1468 850.2 3773 18630.9 0.980 GSM2536589: 5248_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640505 Bulk Tissue 0.737 10.1 43691 1225.8 660 862.5 3437 20204.2 0.985 GSM2536590: 5248_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640506 Bulk Tissue 0.773 11.8 54993 1155.1 328 995.8 3354 21712.1 0.990 GSM2536591: 5250_BA24 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640507 Bulk Tissue 0.715 11.8 36498 1244.8 2187 867.2 3988 14550.3 0.976 GSM2536592: 5250_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640508 Bulk Tissue 0.767 10.3 44489 1215.3 436 959.8 3481 19652.8 0.991 GSM2536593: 5250_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640509 Bulk Tissue 0.743 10.9 43060 1191.0 845 863.3 3782 16901.7 0.986 GSM2536594: 5284_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640510 Bulk Tissue 0.751 11.9 40342 1232.0 1129 875.0 3405 20586.0 0.985 GSM2536595: 5284_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640511 Bulk Tissue 0.718 12.2 36132 1225.8 1874 871.4 3862 15845.5 0.980 GSM2536596: 5358_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640512 Bulk Tissue 0.747 11.3 40974 1245.5 865 913.8 4036 25081.5 0.986 GSM2536597: 5358_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640513 Bulk Tissue 0.753 13.0 41306 1163.3 1290 885.1 3567 18177.5 0.989 GSM2536598: 5404_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640514 Bulk Tissue 0.733 11.8 34545 1202.8 2015 950.3 3559 12349.5 0.985 GSM2536599: 5404_BA24 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640515 Bulk Tissue 0.724 12.6 39148 1210.8 1633 881.6 3581 22041.3 0.982 GSM2536600: 5540_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640516 Bulk Tissue 0.723 10.3 38161 1253.9 1030 877.7 4109 17045.4 0.977 GSM2536601: 5552_BA24 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640517 Bulk Tissue 0.745 11.2 38991 1238.7 1485 884.6 4243 17611.3 0.976 GSM2536602: 5552_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640518 Bulk Tissue 0.756 10.4 48136 1164.3 569 1056.9 3434 21343.5 0.991 GSM2536603: 5552_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640519 Bulk Tissue 0.711 9.6 35022 1261.7 968 849.6 3344 13108.9 0.982 GSM2536604: 5569_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640520 Bulk Tissue 0.729 7.6 41125 1256.9 271 903.6 2098 28823.7 0.987 GSM2536605: 5569_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640521 Bulk Tissue 0.693 11.8 35525 1230.5 1648 849.8 3393 14635.8 0.982 GSM2536606: 5570_BA24 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640522 Bulk Tissue 0.729 10.1 42083 1241.0 667 891.9 4139 20191.7 0.981 GSM2536607: 5570_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640523 Bulk Tissue 0.736 8.3 41842 1247.6 240 952.0 3639 19934.6 0.987 GSM2536608: 5570_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640524 Bulk Tissue 0.734 12.9 40052 1196.5 1704 943.2 3811 16592.4 0.981 GSM2536609: 5628_BA24 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640525 Bulk Tissue 0.723 8.7 35872 1269.1 667 936.1 2204 28352.4 0.981 GSM2536610: 5628_BA9 (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640526 Bulk Tissue 0.733 8.9 44901 1218.5 192 1039.4 3384 17769.9 0.990 GSM2536611: 5628_HC (bisulfite-Seq); Homo sapiens; Bisulfite-Seq
SRX2640527 Bulk Tissue 0.729 11.4 38762 1245.7 1296 876.0 3676 20582.5 0.984 GSM2536612: 5628_NAcc (bisulfite-Seq); Homo sapiens; Bisulfite-Seq

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.