Human Gene ZNF326 (ENST00000340281.9_8) from GENCODE V47lift37
  Description: zinc finger protein 326, transcript variant 1 (from RefSeq NM_182976.4)
Gencode Transcript: ENST00000340281.9_8
Gencode Gene: ENSG00000162664.17_10
Transcript (Including UTRs)
   Position: hg19 chr1:90,460,669-90,501,092 Size: 40,424 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr1:90,460,817-90,493,260 Size: 32,444 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:90,460,669-90,501,092)mRNA (may differ from genome)Protein (582 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZN326_HUMAN
DESCRIPTION: RecName: Full=DBIRD complex subunit ZNF326; AltName: Full=Zinc finger protein 326; AltName: Full=Zinc finger protein interacting with mRNPs and DBC1;
FUNCTION: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. May play a role in neuronal differentiation and is able to bind DNA and activate expression in vitro.
SUBUNIT: Component of the DBIRD complex. Interacts with KIAA1967; the interaction is direct.
INTERACTION: O60477:DBC1; NbExp=5; IntAct=EBI-2560158, EBI-3904864;
SUBCELLULAR LOCATION: Nucleus matrix (By similarity).
SIMILARITY: Belongs to the AKAP95 family.
SIMILARITY: Contains 2 C2H2 AKAP95-type zinc fingers.
SEQUENCE CAUTION: Sequence=AAH52645.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=AAH70341.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=CAH72229.1; Type=Erroneous gene model prediction; Sequence=CAI23074.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 8.84 RPKM in Brain - Spinal cord (cervical c-1)
Total median expression: 217.35 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -67.10148-0.453 Picture PostScript Text
3' UTR -1871.807832-0.239 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007071 - AKAP95
IPR015880 - Znf_C2H2-like

Pfam Domains:
PF04988 - A-kinase anchoring protein 95 (AKAP95)

SCOP Domains:
48371 - ARM repeat
57667 - beta-beta-alpha zinc fingers

ModBase Predicted Comparative 3D Structure on Q5BKZ1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000993 RNA polymerase II core binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0032784 regulation of DNA-templated transcription, elongation
GO:0043484 regulation of RNA splicing

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016363 nuclear matrix
GO:0043231 intracellular membrane-bounded organelle
GO:0044609 DBIRD complex
GO:0005681 spliceosomal complex


-  Descriptions from all associated GenBank mRNAs
  BC052645 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone IMAGE:6526191), partial cds.
BC061521 - Homo sapiens cDNA clone IMAGE:6514772, containing frame-shift errors.
BC038454 - Homo sapiens cDNA clone IMAGE:4472359, containing frame-shift errors.
BC090866 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone MGC:102849 IMAGE:6503526), complete cds.
AK000410 - Homo sapiens cDNA FLJ20403 fis, clone KAT01539.
BC053888 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone IMAGE:6015928), complete cds.
BC014899 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone IMAGE:3912307).
BC070341 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone IMAGE:6107848), partial cds.
BC013102 - Homo sapiens zinc finger protein 326, mRNA (cDNA clone IMAGE:3923723), with apparent retained intron.
AK303707 - Homo sapiens cDNA FLJ50345 complete cds, highly similar to Zinc finger protein 326.
AK297073 - Homo sapiens cDNA FLJ50800 complete cds, highly similar to Zinc finger protein 326.
JD405040 - Sequence 386064 from Patent EP1572962.
KJ900575 - Synthetic construct Homo sapiens clone ccsbBroadEn_09969 ZNF326 gene, encodes complete protein.
JD060943 - Sequence 41967 from Patent EP1572962.
AK124539 - Homo sapiens cDNA FLJ42548 fis, clone BRACE3004996.
JD535674 - Sequence 516698 from Patent EP1572962.
JD295463 - Sequence 276487 from Patent EP1572962.
JD224970 - Sequence 205994 from Patent EP1572962.
JD168735 - Sequence 149759 from Patent EP1572962.
JD064528 - Sequence 45552 from Patent EP1572962.
JD316911 - Sequence 297935 from Patent EP1572962.
JD064527 - Sequence 45551 from Patent EP1572962.
JD355170 - Sequence 336194 from Patent EP1572962.
JD205315 - Sequence 186339 from Patent EP1572962.
JD333240 - Sequence 314264 from Patent EP1572962.
AK126831 - Homo sapiens cDNA FLJ44883 fis, clone BRAMY2036918.

-  Other Names for This Gene
  Alternate Gene Symbols: A8MYX1, B4DLN0, B4E179, ENST00000340281.1, ENST00000340281.2, ENST00000340281.3, ENST00000340281.4, ENST00000340281.5, ENST00000340281.6, ENST00000340281.7, ENST00000340281.8, NM_182976, Q5BKZ1, Q5VW93, Q5VW94, Q5VW96, Q5VW97, Q6NSA2, Q7Z638, Q7Z6C2, uc317vpt.1, uc317vpt.2, ZIRD, ZN326_HUMAN
UCSC ID: ENST00000340281.9_8
RefSeq Accession: NM_182976.4
Protein: Q5BKZ1 (aka ZN326_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.