Human Gene CCN4 (ENST00000220856.6_2) from GENCODE V47lift37
  Description: cellular communication network factor 4, transcript variant 2 (from RefSeq NM_080838.3)
Gencode Transcript: ENST00000220856.6_2
Gencode Gene: ENSG00000104415.14_9
Transcript (Including UTRs)
   Position: hg19 chr8:134,203,380-134,240,307 Size: 36,928 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr8:134,203,388-134,239,953 Size: 36,566 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:134,203,380-134,240,307)mRNA (may differ from genome)Protein (280 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WISP1_HUMAN
DESCRIPTION: RecName: Full=WNT1-inducible-signaling pathway protein 1; Short=WISP-1; AltName: Full=CCN family member 4; AltName: Full=Wnt-1-induced secreted protein; Flags: Precursor;
FUNCTION: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.
SIMILARITY: Belongs to the CCN family.
SIMILARITY: Contains 1 CTCK (C-terminal cystine knot-like) domain.
SIMILARITY: Contains 1 IGFBP N-terminal domain.
SIMILARITY: Contains 1 TSP type-1 domain.
SIMILARITY: Contains 1 VWFC domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.40 RPKM in Cells - Cultured fibroblasts
Total median expression: 121.53 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -83.80354-0.237 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006208 - Cys_knot
IPR006207 - Cys_knot_C
IPR000867 - IGFBP-like
IPR012395 - IGFBP_CNN
IPR017891 - Insulin_GF-bd_Cys-rich_CS
IPR000884 - Thrombospondin_1_rpt
IPR001007 - VWF_C

Pfam Domains:
PF00007 - Cystine-knot domain
PF00090 - Thrombospondin type 1 domain
PF00219 - Insulin-like growth factor binding protein
PF19035 - CCN3 Nov like TSP1 domain

SCOP Domains:
57184 - Growth factor receptor domain
82895 - TSP-1 type 1 repeat

ModBase Predicted Comparative 3D Structure on O95388
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005178 integrin binding
GO:0005520 insulin-like growth factor binding
GO:0008201 heparin binding

Biological Process:
GO:0001558 regulation of cell growth
GO:0007155 cell adhesion
GO:0007165 signal transduction
GO:0007267 cell-cell signaling
GO:0016055 Wnt signaling pathway
GO:0060548 negative regulation of cell death

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LF210294 - JP 2014500723-A/17797: Polycomb-Associated Non-Coding RNAs.
AK293031 - Homo sapiens cDNA FLJ75838 complete cds, highly similar to Homo sapiens WNT1 inducible signaling pathway protein 1 (WISP1), transcript variant 1, mRNA.
AK315822 - Homo sapiens cDNA, FLJ79471 complete cds, highly similar to WNT1-inducible signaling pathway protein 1precursor.
AK301508 - Homo sapiens cDNA FLJ54189 complete cds, highly similar to WNT1-inducible signaling pathway protein 1 precursor.
AY196486 - Homo sapiens WISP1c (WISP1) mRNA, complete cds; alternatively spliced.
AF100779 - Homo sapiens connective tissue growth factor related protein WISP-1 (WISP1) mRNA, complete cds.
AY196487 - Homo sapiens WISP1tc (WISP1) mRNA, complete cds; alternatively spliced.
AY196488 - Homo sapiens WISP1i (WISP1) mRNA, complete cds; alternatively spliced.
BC074840 - Homo sapiens WNT1 inducible signaling pathway protein 1, mRNA (cDNA clone MGC:103937 IMAGE:30915330), complete cds.
BC074841 - Homo sapiens WNT1 inducible signaling pathway protein 1, mRNA (cDNA clone MGC:104098 IMAGE:30915551), complete cds.
AB034725 - Homo sapiens mRNA for WISP-1 variant, complete cds.
KJ904612 - Synthetic construct Homo sapiens clone ccsbBroadEn_14006 WISP1 gene, encodes complete protein.
MA445871 - JP 2018138019-A/17797: Polycomb-Associated Non-Coding RNAs.
JD518452 - Sequence 499476 from Patent EP1572962.
LF336975 - JP 2014500723-A/144478: Polycomb-Associated Non-Coding RNAs.
EF025923 - Homo sapiens WNT1 induced secreted protein 1 splice variant x mRNA, partial cds, alternatively spliced.
JD305087 - Sequence 286111 from Patent EP1572962.
JD403293 - Sequence 384317 from Patent EP1572962.
JD087947 - Sequence 68971 from Patent EP1572962.
JD406392 - Sequence 387416 from Patent EP1572962.
JD195022 - Sequence 176046 from Patent EP1572962.
LF336980 - JP 2014500723-A/144483: Polycomb-Associated Non-Coding RNAs.
MA572552 - JP 2018138019-A/144478: Polycomb-Associated Non-Coding RNAs.
MA572557 - JP 2018138019-A/144483: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8KAG6, CCN4 , CCN4_HUMAN, E7EMM5, ENST00000220856.1, ENST00000220856.2, ENST00000220856.3, ENST00000220856.4, ENST00000220856.5, NM_080838, O95388, Q5JBS6, Q5JBS7, Q5JBS8, Q9HCS3, uc317czv.1, uc317czv.2, WISP1
UCSC ID: ENST00000220856.6_2
RefSeq Accession: NM_080838.3
Protein: O95388 (aka WISP1_HUMAN or WSP1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.