Human Gene UBE2O (ENST00000319380.12_10) from GENCODE V47lift37
  Description: ubiquitin conjugating enzyme E2 O (from RefSeq NM_022066.4)
Gencode Transcript: ENST00000319380.12_10
Gencode Gene: ENSG00000175931.13_16
Transcript (Including UTRs)
   Position: hg19 chr17:74,385,537-74,449,234 Size: 63,698 Total Exon Count: 18 Strand: -
Coding Region
   Position: hg19 chr17:74,387,024-74,449,223 Size: 62,200 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:74,385,537-74,449,234)mRNA (may differ from genome)Protein (1292 aa)
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-  Comments and Description Text from UniProtKB
  ID: UBE2O_HUMAN
DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 O; EC=6.3.2.19; AltName: Full=Ubiquitin carrier protein O; AltName: Full=Ubiquitin-conjugating enzyme E2 of 230 kDa; Short=Ubiquitin-conjugating enzyme E2-230K; AltName: Full=Ubiquitin-protein ligase O;
FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination.
TISSUE SPECIFICITY: Predominantly expressed in skeletal muscle and heart.
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
SEQUENCE CAUTION: Sequence=AAH22237.1; Type=Erroneous initiation; Sequence=AAH25977.1; Type=Erroneous initiation; Sequence=AAH36820.1; Type=Erroneous initiation; Sequence=AAH51868.2; Type=Erroneous initiation; Sequence=BAB14320.1; Type=Erroneous initiation; Sequence=BAB14948.1; Type=Erroneous initiation; Sequence=BAB15313.1; Type=Erroneous initiation; Sequence=BAB21825.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.32 RPKM in Brain - Cerebellum
Total median expression: 500.66 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -529.401487-0.356 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000608 - UBQ-conjugat_E2
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00179 - Ubiquitin-conjugating enzyme

SCOP Domains:
54495 - UBC-like

ModBase Predicted Comparative 3D Structure on Q9C0C9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0031625 ubiquitin protein ligase binding
GO:0061630 ubiquitin protein ligase activity
GO:0061631 ubiquitin conjugating enzyme activity

Biological Process:
GO:0006513 protein monoubiquitination
GO:0016567 protein ubiquitination
GO:0030513 positive regulation of BMP signaling pathway
GO:0042147 retrograde transport, endosome to Golgi
GO:0070534 protein K63-linked ubiquitination

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016604 nuclear body


-  Descriptions from all associated GenBank mRNAs
  AB051521 - Homo sapiens KIAA1734 mRNA for KIAA1734 protein.
AB384279 - Synthetic construct DNA, clone: pF1KSDA1734, Homo sapiens UBE2O gene for ubiquitin-conjugating enzyme E2 O, complete cds, without stop codon, in Flexi system.
BC007822 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone IMAGE:4303741), with apparent retained intron.
BC036820 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone IMAGE:4181549), partial cds.
AK000926 - Homo sapiens cDNA FLJ10064 fis, clone HEMBA1001450.
BC025977 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone IMAGE:3830235), partial cds.
BC022237 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone IMAGE:4180340), complete cds.
BC051868 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone MGC:60241 IMAGE:6162673), complete cds.
AL832432 - Homo sapiens mRNA; cDNA DKFZp762G013 (from clone DKFZp762G013).
AK024657 - Homo sapiens cDNA: FLJ21004 fis, clone CAE03743.
BC004525 - Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA (cDNA clone IMAGE:3502303), partial cds.
AK025999 - Homo sapiens cDNA: FLJ22346 fis, clone HRC06158.
AK022940 - Homo sapiens cDNA FLJ12878 fis, clone NT2RP2003840, weakly similar to HYPOTHETICAL 48.1 KD PROTEIN B0403.2 IN CHROMOSOME X.
JD464144 - Sequence 445168 from Patent EP1572962.
JD230735 - Sequence 211759 from Patent EP1572962.
JD396064 - Sequence 377088 from Patent EP1572962.
JD417416 - Sequence 398440 from Patent EP1572962.
JD418712 - Sequence 399736 from Patent EP1572962.
JD327073 - Sequence 308097 from Patent EP1572962.
JD225306 - Sequence 206330 from Patent EP1572962.
JD039753 - Sequence 20777 from Patent EP1572962.
JD519950 - Sequence 500974 from Patent EP1572962.
JD422376 - Sequence 403400 from Patent EP1572962.
JD554439 - Sequence 535463 from Patent EP1572962.
JD386675 - Sequence 367699 from Patent EP1572962.
JD389977 - Sequence 371001 from Patent EP1572962.
JD098094 - Sequence 79118 from Patent EP1572962.
JD229367 - Sequence 210391 from Patent EP1572962.
AK225412 - Homo sapiens mRNA for likely ortholog of mouse ubiquitin-conjugating enzyme E2-230K variant, clone: HRC06158.
KJ903039 - Synthetic construct Homo sapiens clone ccsbBroadEn_12433 UBE2O gene, encodes complete protein.
KJ903040 - Synthetic construct Homo sapiens clone ccsbBroadEn_12434 UBE2O gene, encodes complete protein.
CU692502 - Synthetic construct Homo sapiens gateway clone IMAGE:100019533 5' read UBE2O mRNA.
CU690876 - Synthetic construct Homo sapiens gateway clone IMAGE:100021540 5' read UBE2O mRNA.
JD156941 - Sequence 137965 from Patent EP1572962.
JD100669 - Sequence 81693 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7511 - protein ubiquitination

Reactome (by CSHL, EBI, and GO)

Protein Q9C0C9 (Reactome details) participates in the following event(s):

R-HSA-983152 Transfer of ubiquitin from E1 to E2
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A6NDU5, ENST00000319380.1, ENST00000319380.10, ENST00000319380.11, ENST00000319380.2, ENST00000319380.3, ENST00000319380.4, ENST00000319380.5, ENST00000319380.6, ENST00000319380.7, ENST00000319380.8, ENST00000319380.9, KIAA1734, NM_022066, Q69YP4, Q6PIZ2, Q86UA4, Q8N425, Q8TBN1, Q9BSW1, Q9C0C9, Q9H6E6, Q9H7E4, Q9H9B2, UBE2O_HUMAN, uc317qqr.1, uc317qqr.2
UCSC ID: ENST00000319380.12_10
RefSeq Accession: NM_022066.4
Protein: Q9C0C9 (aka UBE2O_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.