Human Gene TUBA1B (ENST00000336023.9_7) from GENCODE V47lift37
  Description: tubulin alpha 1b (from RefSeq NM_006082.3)
Gencode Transcript: ENST00000336023.9_7
Gencode Gene: ENSG00000123416.15_14
Transcript (Including UTRs)
   Position: hg19 chr12:49,521,565-49,525,178 Size: 3,614 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr12:49,521,741-49,525,083 Size: 3,343 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:49,521,565-49,525,178)mRNA (may differ from genome)Protein (451 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TBA1B_HUMAN
DESCRIPTION: RecName: Full=Tubulin alpha-1B chain; AltName: Full=Alpha-tubulin ubiquitous; AltName: Full=Tubulin K-alpha-1; AltName: Full=Tubulin alpha-ubiquitous chain;
FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SUBUNIT: Dimer of alpha and beta chains.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity).
PTM: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
PTM: Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).
SIMILARITY: Belongs to the tubulin family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TUBA1B
Diseases sorted by gene-association score: kenny-caffey syndrome (5), hypoparathyroidism-retardation-dysmorphism syndrome (3), distal hereditary motor neuropathy, type ii (2), pachygyria (2), amyotrophic lateral sclerosis 1 (0)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 769.13 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 12273.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -27.7095-0.292 Picture PostScript Text
3' UTR -25.90176-0.147 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002452 - Alpha_tubulin
IPR008280 - Tub_FtsZ_C
IPR000217 - Tubulin
IPR018316 - Tubulin/FtsZ_2-layer-sand-dom
IPR023123 - Tubulin_C
IPR017975 - Tubulin_CS
IPR003008 - Tubulin_FtsZ_GTPase

Pfam Domains:
PF00091 - Tubulin/FtsZ family, GTPase domain
PF03953 - Tubulin C-terminal domain

SCOP Domains:
52490 - Tubulin nucleotide-binding domain-like
55307 - Tubulin C-terminal domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2E4H - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P68363
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003725 double-stranded RNA binding
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005200 structural constituent of cytoskeleton
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0031625 ubiquitin protein ligase binding

Biological Process:
GO:0000226 microtubule cytoskeleton organization
GO:0007017 microtubule-based process
GO:0030705 cytoskeleton-dependent intracellular transport
GO:0051301 cell division
GO:0071353 cellular response to interleukin-4

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0005881 cytoplasmic microtubule
GO:0015630 microtubule cytoskeleton
GO:0043209 myelin sheath
GO:0045121 membrane raft


-  Descriptions from all associated GenBank mRNAs
  BC021063 - Homo sapiens cDNA clone IMAGE:3506521, **** WARNING: chimeric clone ****.
BC015883 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:10192 IMAGE:3909374), complete cds.
BC017004 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:22108 IMAGE:4430457), complete cds.
BC008659 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:9918 IMAGE:3871729), complete cds.
BC001128 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:2457 IMAGE:2964447), complete cds.
BC009512 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:4753 IMAGE:3537301), complete cds.
BC009314 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:16616 IMAGE:4111591), complete cds.
BC006379 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:12832 IMAGE:4100566), complete cds.
BC006481 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:4387 IMAGE:2905305), complete cds.
BC010494 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:17433 IMAGE:3445903), complete cds.
BC018948 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone IMAGE:4130515).
BC021564 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:31891 IMAGE:4579604), complete cds.
BC009509 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:4689 IMAGE:3533174), complete cds.
BC009513 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:4760 IMAGE:3537827), complete cds.
BC000696 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:2215 IMAGE:3349131), complete cds.
BC071904 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:88583 IMAGE:6089096), complete cds.
BC080592 - Homo sapiens tubulin, alpha 4a, mRNA (cDNA clone IMAGE:4541944), **** WARNING: chimeric clone ****.
AK026516 - Homo sapiens cDNA: FLJ22863 fis, clone KAT01983, highly similar to HUMTUBAK human alpha-tubulin mRNA.
AK026632 - Homo sapiens cDNA: FLJ22979 fis, clone KAT11379, highly similar to HUMTUBAK human alpha-tubulin mRNA.
DQ400107 - Homo sapiens alpha tubulin (K-ALPHA-1) mRNA, complete cds.
K00558 - human alpha-tubulin mRNA, complete cds.
Z36832 - H.sapiens (xs31) mRNA, 835bp.
AK293337 - Homo sapiens cDNA FLJ60097 complete cds, highly similar to Tubulin alpha-ubiquitous chain.
AK056693 - Homo sapiens cDNA FLJ32131 fis, clone PEBLM2000267, highly similar to Tubulin alpha-ubiquitous chain.
BC011572 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:20231 IMAGE:4561240), complete cds.
AK057773 - Homo sapiens cDNA FLJ25044 fis, clone CBL03486, highly similar to TUBULIN ALPHA-1 CHAIN.
AK094717 - Homo sapiens cDNA FLJ37398 fis, clone BRAMY2027467, highly similar to Tubulin alpha-ubiquitous chain.
AK098357 - Homo sapiens cDNA FLJ25491 fis, clone CBR00773, highly similar to TUBULIN ALPHA-1 CHAIN.
AK098635 - Homo sapiens cDNA FLJ25769 fis, clone TST06404, highly similar to TUBULIN ALPHA-1 CHAIN.
AK098771 - Homo sapiens cDNA FLJ25905 fis, clone CBR04587, highly similar to TUBULIN ALPHA-1 CHAIN.
AK289354 - Homo sapiens cDNA FLJ78587 complete cds.
BC030820 - Homo sapiens tubulin, alpha 1b, mRNA (cDNA clone MGC:23826 IMAGE:4277263), complete cds.
JD295010 - Sequence 276034 from Patent EP1572962.
JD502773 - Sequence 483797 from Patent EP1572962.
JD560601 - Sequence 541625 from Patent EP1572962.
JD251724 - Sequence 232748 from Patent EP1572962.
AF081484 - Homo sapiens alpha-tubulin isoform 1 mRNA, complete cds.
DQ891498 - Synthetic construct clone IMAGE:100004128; FLH177498.01X; RZPDo839A11126D alpha tubulin (K-ALPHA-1) gene, encodes complete protein.
KJ893019 - Synthetic construct Homo sapiens clone ccsbBroadEn_02413 TUBA1B gene, encodes complete protein.
KJ906036 - Synthetic construct Homo sapiens clone ccsbBroadEn_15706 TUBA1B gene, encodes complete protein.
KJ906037 - Synthetic construct Homo sapiens clone ccsbBroadEn_15707 TUBA1B gene, encodes complete protein.
DQ894689 - Synthetic construct Homo sapiens clone IMAGE:100009149; FLH177494.01L; RZPDo839A11125D alpha tubulin (K-ALPHA-1) gene, encodes complete protein.
KJ906038 - Synthetic construct Homo sapiens clone ccsbBroadEn_15708 TUBA1B gene, encodes complete protein.
AB464324 - Synthetic construct DNA, clone: pF1KB8265, Homo sapiens TUBA1B gene for tubulin, alpha 1b, without stop codon, in Flexi system.
JD440596 - Sequence 421620 from Patent EP1572962.
JD220127 - Sequence 201151 from Patent EP1572962.
JD545462 - Sequence 526486 from Patent EP1572962.
JD060811 - Sequence 41835 from Patent EP1572962.
CU692366 - Synthetic construct Homo sapiens gateway clone IMAGE:100018133 5' read TUBA1B mRNA.
JD026159 - Sequence 7183 from Patent EP1572962.
JD026858 - Sequence 7882 from Patent EP1572962.
JD317441 - Sequence 298465 from Patent EP1572962.
JD036090 - Sequence 17114 from Patent EP1572962.
CU677793 - Synthetic construct Homo sapiens gateway clone IMAGE:100017297 5' read TUBA1B mRNA.
JD024118 - Sequence 5142 from Patent EP1572962.
JD024119 - Sequence 5143 from Patent EP1572962.
JD027868 - Sequence 8892 from Patent EP1572962.
JD033115 - Sequence 14139 from Patent EP1572962.
CU687192 - Synthetic construct Homo sapiens gateway clone IMAGE:100023159 5' read TUBA1B mRNA.
JD025802 - Sequence 6826 from Patent EP1572962.
JD029721 - Sequence 10745 from Patent EP1572962.
CU679019 - Synthetic construct Homo sapiens gateway clone IMAGE:100017298 5' read TUBA1B mRNA.
JD029285 - Sequence 10309 from Patent EP1572962.
JD027061 - Sequence 8085 from Patent EP1572962.
JD035518 - Sequence 16542 from Patent EP1572962.
JD024485 - Sequence 5509 from Patent EP1572962.
DQ589802 - Homo sapiens piRNA piR-56914, complete sequence.
JD020410 - Sequence 1434 from Patent EP1572962.
AY533484 - Homo sapiens tubulin alpha-1 (TUBA1) mRNA, partial cds.
JD032220 - Sequence 13244 from Patent EP1572962.
S62639 - alpha-tubulin {EST} [human, mRNA Partial, 337 nt].
JD020374 - Sequence 1398 from Patent EP1572962.
JD035670 - Sequence 16694 from Patent EP1572962.
DL490632 - Novel nucleic acids.
JD025908 - Sequence 6932 from Patent EP1572962.
JD023766 - Sequence 4790 from Patent EP1572962.
JD034886 - Sequence 15910 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P68363 (Reactome details) participates in the following event(s):

R-HSA-389954 Hydrolysis of ATP and release of tubulin folding intermediate from CCT/TriC
R-HSA-389961 ADP is exchanged for ATP in the (ADP:CCT/TriC):tubulin complex
R-HSA-389972 alpha-tubulin:GTP + Cofactor B -> alpha-tubulin:GTP: Cofactor B
R-HSA-389978 alpha-tubulin:GTP + Cofactor E -> alpha-tubulin:GTP:Cofactor E
R-HSA-389974 Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E:Cofactor C-> Beta-tubulin:GDP :alpha-tubulin:GTP heterodimer +Cofactor E+ Cofactor D+ Cofactor C+ Pi
R-HSA-8955712 TTCP hydrolyzes the terminal L-Tyr residue from alpha-tubulin
R-HSA-8955706 TTL ligates L-Tyr to the carboxy terminus of alpha-tubulin
R-HSA-389963 alpha-tubulin:GTP:Cofactor B +Cofactor E -> alpha-tubulin:GTP: Cofactor E +Cofactor B
R-HSA-389976 Beta-tubulin:GTP:Cofactor D+alpha-tubulin:GTP:Cofactor E-> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E
R-HSA-389964 Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E+ Cofactor C-> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E:Cofactor C
R-HSA-389960 Formation of tubulin folding intermediates by CCT/TriC
R-HSA-389977 Post-chaperonin tubulin folding pathway
R-HSA-8955332 Carboxyterminal post-translational modifications of tubulin
R-HSA-389958 Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
R-HSA-391251 Protein folding
R-HSA-597592 Post-translational protein modification
R-HSA-390466 Chaperonin-mediated protein folding
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000336023.1, ENST00000336023.2, ENST00000336023.3, ENST00000336023.4, ENST00000336023.5, ENST00000336023.6, ENST00000336023.7, ENST00000336023.8, NM_006082, P04687, P05209, P68363, Q27I68, Q8WU19, TBA1B_HUMAN, uc317ukm.1, uc317ukm.2
UCSC ID: ENST00000336023.9_7
RefSeq Accession: NM_006082.3
Protein: P68363 (aka TBA1B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.