Human Gene TOLLIP (ENST00000317204.11_7) from GENCODE V47lift37
  Description: toll interacting protein, transcript variant 1 (from RefSeq NM_019009.4)
Gencode Transcript: ENST00000317204.11_7
Gencode Gene: ENSG00000078902.16_10
Transcript (Including UTRs)
   Position: hg19 chr11:1,295,601-1,330,862 Size: 35,262 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr11:1,298,269-1,330,728 Size: 32,460 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:1,295,601-1,330,862)mRNA (may differ from genome)Protein (274 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TOLIP_HUMAN
DESCRIPTION: RecName: Full=Toll-interacting protein;
FUNCTION: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
SUBUNIT: Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2.
INTERACTION: Q05048:CSTF1; NbExp=2; IntAct=EBI-74615, EBI-1789619; Q15038:DAZAP2; NbExp=3; IntAct=EBI-74615, EBI-724310; Q86Y13:DZIP3; NbExp=2; IntAct=EBI-74615, EBI-948630; P19525:EIF2AK2; NbExp=2; IntAct=EBI-74615, EBI-640775; O43187:IRAK2; NbExp=2; IntAct=EBI-74615, EBI-447733; Q86TG7:PEG10; NbExp=2; IntAct=EBI-74615, EBI-2858265; O75674:TOM1L1; NbExp=3; IntAct=EBI-74615, EBI-712991; Q96S55:WRNIP1; NbExp=2; IntAct=EBI-74615, EBI-2513471;
SUBCELLULAR LOCATION: Cytoplasm (Probable).
PTM: Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
SIMILARITY: Belongs to the tollip family.
SIMILARITY: Contains 1 C2 domain.
SIMILARITY: Contains 1 CUE domain.
SEQUENCE CAUTION: Sequence=BAB14283.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TOLLIP
Diseases sorted by gene-association score: ileum cancer (11), lymphoproliferative syndrome, x-linked, 1 (7), pulmonary fibrosis, idiopathic (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 24.61 RPKM in Testis
Total median expression: 426.19 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -72.50134-0.541 Picture PostScript Text
3' UTR -1043.302668-0.391 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000008 - C2_Ca-dep
IPR008973 - C2_Ca/lipid-bd_dom_CaLB
IPR003892 - CUE
IPR009060 - UBA-like

Pfam Domains:
PF00168 - C2 domain
PF02845 - CUE domain

SCOP Domains:
46934 - UBA-like
49562 - C2 domain (Calcium/lipid-binding domain, CaLB)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1WGL - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9H0E2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0005150 interleukin-1, Type I receptor binding
GO:0005515 protein binding
GO:0019900 kinase binding
GO:0031624 ubiquitin conjugating enzyme binding
GO:0031625 ubiquitin protein ligase binding
GO:0032183 SUMO binding
GO:0035325 Toll-like receptor binding
GO:0043130 ubiquitin binding

Biological Process:
GO:0002376 immune system process
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006914 autophagy
GO:0006954 inflammatory response
GO:0007165 signal transduction
GO:0007267 cell-cell signaling
GO:0016310 phosphorylation
GO:0030855 epithelial cell differentiation
GO:0033235 positive regulation of protein sumoylation
GO:0035556 intracellular signal transduction
GO:0036010 protein localization to endosome
GO:0043312 neutrophil degranulation
GO:0045087 innate immune response
GO:0045321 leukocyte activation
GO:0070498 interleukin-1-mediated signaling pathway

Cellular Component:
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016604 nuclear body
GO:0035578 azurophil granule lumen
GO:0035580 specific granule lumen
GO:0045092 interleukin-18 receptor complex
GO:0045323 interleukin-1 receptor complex
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF384316 - JP 2014500723-A/191819: Polycomb-Associated Non-Coding RNAs.
AL136835 - Homo sapiens mRNA; cDNA DKFZp434D152 (from clone DKFZp434D152).
AK127123 - Homo sapiens cDNA FLJ45180 fis, clone BRAWH3047565, highly similar to Toll-interacting protein.
AB209541 - Homo sapiens mRNA for toll interacting protein variant protein.
AK096693 - Homo sapiens cDNA FLJ39374 fis, clone PEBLM2008576, highly similar to Homo sapiens mRNA for TOLLIP protein.
AK022871 - Homo sapiens cDNA FLJ12809 fis, clone NT2RP2002408, highly similar to Homo sapiens mRNA for TOLLIP protein.
AK090850 - Homo sapiens cDNA FLJ33531 fis, clone BRAMY2007255, highly similar to Toll-interacting protein.
BC018272 - Homo sapiens toll interacting protein, mRNA (cDNA clone MGC:16867 IMAGE:3452585), complete cds.
BC012057 - Homo sapiens toll interacting protein, mRNA (cDNA clone MGC:19882 IMAGE:4647828), complete cds.
BC004420 - Homo sapiens toll interacting protein, mRNA (cDNA clone MGC:1194 IMAGE:3356339), complete cds.
AJ242972 - Homo sapiens mRNA for TOLLIP protein.
CR533477 - Homo sapiens full open reading frame cDNA clone RZPDo834B0120D for gene TOLLIP, toll interacting protein; complete cds, incl. stopcodon.
AY730683 - Homo sapiens toll interacting protein (TOLLIP) mRNA, complete cds.
DQ891802 - Synthetic construct clone IMAGE:100004432; FLH180090.01X; RZPDo839F10132D toll interacting protein (TOLLIP) gene, encodes complete protein.
AK315592 - Homo sapiens cDNA, FLJ96670, Homo sapiens toll interacting protein (TOLLIP), mRNA.
KJ894026 - Synthetic construct Homo sapiens clone ccsbBroadEn_03420 TOLLIP gene, encodes complete protein.
KJ898988 - Synthetic construct Homo sapiens clone ccsbBroadEn_08382 TOLLIP gene, encodes complete protein.
DQ894986 - Synthetic construct Homo sapiens clone IMAGE:100009446; FLH180086.01L; RZPDo839F10131D toll interacting protein (TOLLIP) gene, encodes complete protein.
AB590101 - Synthetic construct DNA, clone: pFN21AB8351, Homo sapiens TOLLIP gene for toll interacting protein, without stop codon, in Flexi system.
AM393373 - Synthetic construct Homo sapiens clone IMAGE:100002468 for hypothetical protein (TOLLIP gene).
MA619893 - JP 2018138019-A/191819: Polycomb-Associated Non-Coding RNAs.
BX648136 - Homo sapiens mRNA; cDNA DKFZp686D0146 (from clone DKFZp686D0146).
JD315437 - Sequence 296461 from Patent EP1572962.
JD455336 - Sequence 436360 from Patent EP1572962.
JD406158 - Sequence 387182 from Patent EP1572962.
JD162363 - Sequence 143387 from Patent EP1572962.
JD070765 - Sequence 51789 from Patent EP1572962.
JD105229 - Sequence 86253 from Patent EP1572962.
JD383809 - Sequence 364833 from Patent EP1572962.
JD105228 - Sequence 86252 from Patent EP1572962.
JD424056 - Sequence 405080 from Patent EP1572962.
JD403082 - Sequence 384106 from Patent EP1572962.
JD279307 - Sequence 260331 from Patent EP1572962.
JD269874 - Sequence 250898 from Patent EP1572962.
JD260837 - Sequence 241861 from Patent EP1572962.
JD171015 - Sequence 152039 from Patent EP1572962.
LF370618 - JP 2014500723-A/178121: Polycomb-Associated Non-Coding RNAs.
JD163187 - Sequence 144211 from Patent EP1572962.
JD210568 - Sequence 191592 from Patent EP1572962.
JD451340 - Sequence 432364 from Patent EP1572962.
JD422983 - Sequence 404007 from Patent EP1572962.
JD122197 - Sequence 103221 from Patent EP1572962.
JD556660 - Sequence 537684 from Patent EP1572962.
JD186181 - Sequence 167205 from Patent EP1572962.
DQ596089 - Homo sapiens piRNA piR-34155, complete sequence.
JD487668 - Sequence 468692 from Patent EP1572962.
JD197174 - Sequence 178198 from Patent EP1572962.
JD230180 - Sequence 211204 from Patent EP1572962.
JD219838 - Sequence 200862 from Patent EP1572962.
JD114654 - Sequence 95678 from Patent EP1572962.
DQ590516 - Homo sapiens piRNA piR-57628, complete sequence.
JD456290 - Sequence 437314 from Patent EP1572962.
JD558169 - Sequence 539193 from Patent EP1572962.
JD419115 - Sequence 400139 from Patent EP1572962.
JD424307 - Sequence 405331 from Patent EP1572962.
JD060209 - Sequence 41233 from Patent EP1572962.
JD268305 - Sequence 249329 from Patent EP1572962.
JD445257 - Sequence 426281 from Patent EP1572962.
JD216078 - Sequence 197102 from Patent EP1572962.
LF370619 - JP 2014500723-A/178122: Polycomb-Associated Non-Coding RNAs.
JD232550 - Sequence 213574 from Patent EP1572962.
JD545124 - Sequence 526148 from Patent EP1572962.
JD190414 - Sequence 171438 from Patent EP1572962.
JD445163 - Sequence 426187 from Patent EP1572962.
JD114815 - Sequence 95839 from Patent EP1572962.
JD169118 - Sequence 150142 from Patent EP1572962.
JD186076 - Sequence 167100 from Patent EP1572962.
JD155040 - Sequence 136064 from Patent EP1572962.
LF370620 - JP 2014500723-A/178123: Polycomb-Associated Non-Coding RNAs.
JD395028 - Sequence 376052 from Patent EP1572962.
JD458959 - Sequence 439983 from Patent EP1572962.
JD210497 - Sequence 191521 from Patent EP1572962.
JD554564 - Sequence 535588 from Patent EP1572962.
JD461729 - Sequence 442753 from Patent EP1572962.
JD272209 - Sequence 253233 from Patent EP1572962.
JD280567 - Sequence 261591 from Patent EP1572962.
JD148207 - Sequence 129231 from Patent EP1572962.
JD230931 - Sequence 211955 from Patent EP1572962.
LF370621 - JP 2014500723-A/178124: Polycomb-Associated Non-Coding RNAs.
JD161323 - Sequence 142347 from Patent EP1572962.
JD253534 - Sequence 234558 from Patent EP1572962.
JD097710 - Sequence 78734 from Patent EP1572962.
JD205711 - Sequence 186735 from Patent EP1572962.
JD272448 - Sequence 253472 from Patent EP1572962.
MA606195 - JP 2018138019-A/178121: Polycomb-Associated Non-Coding RNAs.
MA606196 - JP 2018138019-A/178122: Polycomb-Associated Non-Coding RNAs.
MA606197 - JP 2018138019-A/178123: Polycomb-Associated Non-Coding RNAs.
MA606198 - JP 2018138019-A/178124: Polycomb-Associated Non-Coding RNAs.
LF370624 - JP 2014500723-A/178127: Polycomb-Associated Non-Coding RNAs.
LF370625 - JP 2014500723-A/178128: Polycomb-Associated Non-Coding RNAs.
LF370626 - JP 2014500723-A/178129: Polycomb-Associated Non-Coding RNAs.
DL491534 - Novel nucleic acids.
DL490140 - Novel nucleic acids.
MA606201 - JP 2018138019-A/178127: Polycomb-Associated Non-Coding RNAs.
MA606202 - JP 2018138019-A/178128: Polycomb-Associated Non-Coding RNAs.
MA606203 - JP 2018138019-A/178129: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H0E2 (Reactome details) participates in the following event(s):

R-HSA-446868 The Interleukin 1 receptor complex binds Tollip
R-HSA-446894 TRAF6 binding leads to IRAK1:TRAF6 release
R-HSA-446692 IRAK1 binds to MYD88 within the IL1R complex
R-HSA-446634 IRAK4 is activated by autophosphorylation
R-HSA-6798751 Exocytosis of azurophil granule lumen proteins
R-HSA-6798749 Exocytosis of specific granule lumen proteins
R-HSA-446701 IRAK4-activated IRAK1 autophosphorylates
R-HSA-446694 IRAK4 phosphorylates IRAK1
R-HSA-446862 Hyperphosphorylated IRAK1 associates with TRAF6
R-HSA-9020702 Interleukin-1 signaling
R-HSA-446652 Interleukin-1 family signaling
R-HSA-6798695 Neutrophil degranulation
R-HSA-449147 Signaling by Interleukins
R-HSA-168249 Innate Immune System
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B3KXC6, ENST00000317204.1, ENST00000317204.10, ENST00000317204.2, ENST00000317204.3, ENST00000317204.4, ENST00000317204.5, ENST00000317204.6, ENST00000317204.7, ENST00000317204.8, ENST00000317204.9, NM_019009, Q9H0E2, Q9H9E6, Q9UJ69, TOLIP_HUMAN, uc317qen.1, uc317qen.2
UCSC ID: ENST00000317204.11_7
RefSeq Accession: NM_019009.4
Protein: Q9H0E2 (aka TOLIP_HUMAN or TLIP_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.