ID:TMEDA_HUMAN DESCRIPTION: RecName: Full=Transmembrane emp24 domain-containing protein 10; AltName: Full=21 kDa transmembrane-trafficking protein; AltName: Full=S31III125; Short=S31I125; AltName: Full=Tmp-21-I; AltName: Full=Transmembrane protein Tmp21; AltName: Full=p23; AltName: Full=p24 family protein delta-1; Short=p24delta1; AltName: Full=p24delta; Flags: Precursor; FUNCTION: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. SUBUNIT: Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP- bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D (By similarity). Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand (By similarity). Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin- dependent gamma-secretase complex. INTERACTION: Q92542:NCSTN; NbExp=5; IntAct=EBI-998422, EBI-998440; P49768:PSEN1; NbExp=3; IntAct=EBI-998422, EBI-297277; Q9NZ42:PSENEN; NbExp=3; IntAct=EBI-998422, EBI-998468; P17706-1:PTPN2; NbExp=5; IntAct=EBI-998422, EBI-4409481; Q15363:TMED2; NbExp=7; IntAct=EBI-998422, EBI-998485; SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Melanosome. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type I membrane protein (By similarity). Cell membrane (By similarity). Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway. TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain (By similarity). MISCELLANEOUS: Ectopic expression of TMED10 alone does not result in its proper cis-Golgi network localization. Coexpression of TMED2 is necessary, and coexpression of TMED3 and /or TMED9 is facilitating localization. SIMILARITY: Belongs to the EMP24/GP25L family. SIMILARITY: Contains 1 GOLD domain. SEQUENCE CAUTION: Sequence=AAC42003.1; Type=Frameshift; Positions=Several; Sequence=CAD66561.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P49755
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001822 kidney development GO:0006886 intracellular protein transport GO:0006888 ER to Golgi vesicle-mediated transport GO:0006890 retrograde vesicle-mediated transport, Golgi to ER GO:0007030 Golgi organization GO:0015031 protein transport GO:0016192 vesicle-mediated transport GO:0035459 cargo loading into vesicle GO:0035964 COPI-coated vesicle budding GO:0043279 response to alkaloid GO:0045055 regulated exocytosis GO:0048199 vesicle targeting, to, from or within Golgi GO:0048205 COPI coating of Golgi vesicle GO:0048208 COPII vesicle coating GO:0051259 protein oligomerization GO:1902003 regulation of beta-amyloid formation
AL832012 - Homo sapiens mRNA; cDNA DKFZp451D084 (from clone DKFZp451D084); complete cds. BX248754 - human full-length cDNA 5-PRIME end of clone CS0DF013YM24 of Fetal brain of Homo sapiens (human). BC001496 - Homo sapiens transmembrane emp24-like trafficking protein 10 (yeast), mRNA (cDNA clone MGC:1979 IMAGE:2959718), complete cds. BC001825 - Homo sapiens transmembrane emp24-like trafficking protein 10 (yeast), mRNA (cDNA clone MGC:3798 IMAGE:2959718), complete cds. AJ004913 - Homo sapiens mRNA for integral membrane protein Tmp21-I (p23). L40397 - Homo sapiens (clone S31i125) mRNA, 3' end of cds. AK296800 - Homo sapiens cDNA FLJ53754 complete cds, highly similar to Transmembrane emp24 domain-containing protein 10 precursor. AK310371 - Homo sapiens cDNA, FLJ17413. AK222972 - Homo sapiens mRNA for Full-length cDNA 5-PRIME end of clone CS0DF013YM24 of Fetal brain of Homo sapiens (human) (Fragment) variant, clone: HSI03636. HM005519 - Homo sapiens clone HTL-T-206 testicular tissue protein Li 206 mRNA, complete cds. X97442 - H.sapiens mRNA for transmembrane protein Tmp21-I. AK312384 - Homo sapiens cDNA, FLJ92714, Homo sapiens transmembrane trafficking protein (TMP21), mRNA. JF432606 - Synthetic construct Homo sapiens clone IMAGE:100073843 transmembrane emp24-like trafficking protein 10 (yeast) (TMED10) gene, encodes complete protein. KJ893186 - Synthetic construct Homo sapiens clone ccsbBroadEn_02580 TMED10 gene, encodes complete protein. JD225312 - Sequence 206336 from Patent EP1572962. JD091421 - Sequence 72445 from Patent EP1572962. L40391 - Homo sapiens (clone s153) mRNA fragment. AK023615 - Homo sapiens cDNA FLJ13553 fis, clone PLACE1007454. AK056156 - Homo sapiens cDNA FLJ31594 fis, clone NT2RI2002507. JD476742 - Sequence 457766 from Patent EP1572962. JD019369 - Sequence 393 from Patent EP1572962. JD031680 - Sequence 12704 from Patent EP1572962. JD082622 - Sequence 63646 from Patent EP1572962. AK302920 - Homo sapiens cDNA FLJ52032 complete cds, highly similar to Transmembrane emp24 domain-containing protein 10 precursor. JD560368 - Sequence 541392 from Patent EP1572962. JD565501 - Sequence 546525 from Patent EP1572962. AK308864 - Homo sapiens cDNA, FLJ98905. JD433282 - Sequence 414306 from Patent EP1572962. JD528247 - Sequence 509271 from Patent EP1572962. JD378898 - Sequence 359922 from Patent EP1572962. DQ584114 - Homo sapiens piRNA piR-51226, complete sequence. JD239995 - Sequence 221019 from Patent EP1572962. JD470242 - Sequence 451266 from Patent EP1572962. JD475606 - Sequence 456630 from Patent EP1572962. JD025635 - Sequence 6659 from Patent EP1572962. JD036325 - Sequence 17349 from Patent EP1572962. JD035185 - Sequence 16209 from Patent EP1572962. JD025039 - Sequence 6063 from Patent EP1572962. LF209604 - JP 2014500723-A/17107: Polycomb-Associated Non-Coding RNAs. LF330103 - JP 2014500723-A/137606: Polycomb-Associated Non-Coding RNAs. JD026515 - Sequence 7539 from Patent EP1572962. JD035946 - Sequence 16970 from Patent EP1572962. MA565680 - JP 2018138019-A/137606: Polycomb-Associated Non-Coding RNAs. MA445181 - JP 2018138019-A/17107: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P49755 (Reactome details) participates in the following event(s):
R-HSA-5694410 TMED2:TMED10 tetramer binds GPI anchored proteins R-HSA-6807875 ARFGAP, cargo, v-SNAREs and p24 proteins bind nascent COPI complex R-HSA-6811417 ARFGAP, cargo, vSNARES and p24 proteins bind COPI vesicles at Golgi R-HSA-5694522 Inner coat assembly and cargo binding R-HSA-5694527 Loss of SAR1B GTPase R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs R-HSA-5694446 BET1:GOSR2:STX5 bind v-SNARES on tethered vesicle R-HSA-6807877 ARFGAPs stimulate ARF GTPase activity R-HSA-6811418 ARFGAPs stimulate ARF GTPase activity at the Golgi membrane R-HSA-6809003 ERGIC-to-Golgi vesicles bind dynein:dynactin R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules R-HSA-5694417 SEC16 complex binds SAR1B:GTP:SEC23:SEC24 R-HSA-5694441 CSNK1D phosphorylates SEC23 R-HSA-5694439 COPII coat binds TRAPPCII and RAB1:GDP R-HSA-203973 Vesicle budding R-HSA-6809011 cis-Golgi t-SNAREs bind YKT6 on tethered vesicle R-HSA-6809006 Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex R-HSA-204008 SEC31:SEC13 and v-SNARE recruitment R-HSA-5694418 RAB1:GTP binds USO1 and GORASP1:GOLGA2 R-HSA-5694409 Nucleotide exchange on RAB1 R-HSA-5694530 Cargo concentration in the ER R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-6807878 COPI-mediated anterograde transport R-HSA-204005 COPII-mediated vesicle transport R-HSA-199991 Membrane Trafficking R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-5653656 Vesicle-mediated transport R-HSA-446203 Asparagine N-linked glycosylation R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins
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Common Gene Haplotype Alleles 
