Human Gene TLE3 (ENST00000451782.7_7) from GENCODE V47lift37
  Description: TLE family member 3, transcriptional corepressor, transcript variant 2 (from RefSeq NM_001105192.3)
Gencode Transcript: ENST00000451782.7_7
Gencode Gene: ENSG00000140332.18_16
Transcript (Including UTRs)
   Position: hg19 chr15:70,340,129-70,390,256 Size: 50,128 Total Exon Count: 20 Strand: -
Coding Region
   Position: hg19 chr15:70,342,436-70,389,137 Size: 46,702 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:70,340,129-70,390,256)mRNA (may differ from genome)Protein (769 aa)
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-  Comments and Description Text from UniProtKB
  ID: TLE3_HUMAN
DESCRIPTION: RecName: Full=Transducin-like enhancer protein 3; AltName: Full=Enhancer of split groucho-like protein 3; Short=ESG3;
FUNCTION: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).
SUBUNIT: Homotetramer and heterooligomer with other family members. Binds LEF1, TCF7 and TCF7L1 (By similarity). Binds FOXA2. Interacts with XIAP/BIRC4 and TCF7L2/TCF4.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Placenta and lung.
PTM: Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.
SIMILARITY: Belongs to the WD repeat Groucho/TLE family.
SIMILARITY: Contains 7 WD repeats.
SEQUENCE CAUTION: Sequence=BAB13373.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 34.13 RPKM in Whole Blood
Total median expression: 424.08 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -561.101119-0.501 Picture PostScript Text
3' UTR -851.502307-0.369 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005617 - Groucho/TLE_N
IPR009146 - Groucho_enhance
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat
PF03920 - Groucho/TLE N-terminal Q-rich domain

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50956 - Thermostable phytase (3-phytase)
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q04726
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0007165 signal transduction
GO:0009887 animal organ morphogenesis
GO:0016055 Wnt signaling pathway
GO:1904837 beta-catenin-TCF complex assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:1990907 beta-catenin-TCF complex


-  Descriptions from all associated GenBank mRNAs
  AB046767 - Homo sapiens KIAA1547 mRNA for KIAA1547 protein.
BC041831 - Homo sapiens transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:43497 IMAGE:5268249), complete cds.
BC015729 - Homo sapiens transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone IMAGE:4906791), with apparent retained intron.
BC043247 - Homo sapiens transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:44362 IMAGE:5296274), complete cds.
AK096779 - Homo sapiens cDNA FLJ39460 fis, clone PROST2011482, highly similar to Transducin-like enhancer protein 3.
AK298482 - Homo sapiens cDNA FLJ58800 complete cds, highly similar to Transducin-like enhancer protein 3.
AK304459 - Homo sapiens cDNA FLJ58232 complete cds, highly similar to Transducin-like enhancer protein 3.
M99438 - Human transducin-like enhancer protein (TLE3) mRNA, complete cds.
AY583459 - Homo sapiens transducin-like enhancer of split 3 splice variant 1 (TLE3) mRNA, complete cds, alternatively spliced.
AY583460 - Homo sapiens transducin-like enhancer of split 3 splice variant 2 (TLE3) mRNA, complete cds, alternatively spliced.
AK315058 - Homo sapiens cDNA, FLJ96010.
HQ257894 - Synthetic construct Homo sapiens clone IMAGE:100072203 Unknown protein gene, encodes complete protein.
KJ901797 - Synthetic construct Homo sapiens clone ccsbBroadEn_11191 TLE3 gene, encodes complete protein.
AB384233 - Synthetic construct DNA, clone: pF1KSDA1547, Homo sapiens TLE3 gene for transducin-like enhancer protein 3, complete cds, without stop codon, in Flexi system.
BC012004 - Homo sapiens transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone IMAGE:3865641), with apparent retained intron.
AK000083 - Homo sapiens cDNA FLJ20076 fis, clone COL02766.
BC015421 - Homo sapiens transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone IMAGE:4397534), with apparent retained intron.
AK001258 - Homo sapiens cDNA FLJ10396 fis, clone NT2RM4000290, highly similar to Transducin-like enhancer protein 3.
AB209672 - Homo sapiens mRNA for Transducin-like enhancer of split 3 splice variant 1 variant protein.
AY566265 - Homo sapiens transducin-like enhancer of split 3 short isoform (TLE3) mRNA, partial cds, alternatively spliced.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q04726 (Reactome details) participates in the following event(s):

R-HSA-1912359 HES1 binds TLE
R-NUL-2065510 rHes1 binds TLE
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-1980143 Signaling by NOTCH1
R-HSA-157118 Signaling by NOTCH
R-HSA-162582 Signal Transduction
R-HSA-4641229 TLE oligomerizes
R-HSA-4649028 TLE forms non-functional complexes with AES
R-HSA-3322431 XIAP binds TLE
R-HSA-8944349 TLE tetramers bind TCF/LEF at WNT promoters
R-HSA-3299569 Beta-catenin displaces TLE:HDAC1 from TCF/LEF
R-HSA-3322434 XIAP dissociates from ub-TLE
R-HSA-3322429 XIAP monoubiquinates TLE
R-HSA-4641231 TLE recruits HDAC1 to WNT promoters
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-4641265 Repression of WNT target genes
R-HSA-8939211 ESR-mediated signaling
R-HSA-3769402 Deactivation of the beta-catenin transactivating complex
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-9006931 Signaling by Nuclear Receptors
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B4DPT0, E9PD64, ENST00000451782.1, ENST00000451782.2, ENST00000451782.3, ENST00000451782.4, ENST00000451782.5, ENST00000451782.6, F8W964, KIAA1547, NM_001105192, Q04726, Q6PI57, Q8IVV6, Q8WVR2, Q9HCM5, TLE3_HUMAN, uc320tdl.1, uc320tdl.2
UCSC ID: ENST00000451782.7_7
RefSeq Accession: NM_001105192.3
Protein: Q04726 (aka TLE3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.