Human Gene TARS3 (ENST00000335968.8_9) from GENCODE V47lift37
  Description: threonyl-tRNA synthetase 3 (from RefSeq NM_152334.3)
Gencode Transcript: ENST00000335968.8_9
Gencode Gene: ENSG00000185418.16_14
Transcript (Including UTRs)
   Position: hg19 chr15:102,193,799-102,264,676 Size: 70,878 Total Exon Count: 19 Strand: -
Coding Region
   Position: hg19 chr15:102,194,785-102,264,590 Size: 69,806 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:102,193,799-102,264,676)mRNA (may differ from genome)Protein (802 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SYTC2_HUMAN
DESCRIPTION: RecName: Full=Probable threonine--tRNA ligase 2, cytoplasmic; EC=6.1.1.3; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS; AltName: Full=Threonyl-tRNA synthetase-like protein 2;
CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
SEQUENCE CAUTION: Sequence=BAB71554.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.13 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 327.39 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -39.8086-0.463 Picture PostScript Text
3' UTR -227.20986-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002314 - aa-tRNA-synt_IIb_cons-dom
IPR006195 - aa-tRNA-synth_II
IPR004154 - Anticodon-bd
IPR012675 - Beta-grasp_dom
IPR004095 - TGS
IPR012676 - TGS-like
IPR002320 - Thr-tRNA-ligase_IIa
IPR018163 - Thr/Ala-tRNA-synth_IIc_edit
IPR012947 - tRNA_SAD

Pfam Domains:
PF00587 - tRNA synthetase class II core domain (G, H, P, S and T)
PF02824 - TGS domain
PF03129 - Anticodon binding domain
PF07973 - Threonyl and Alanyl tRNA synthetase second additional domain

SCOP Domains:
52954 - Class II aaRS ABD-related
55681 - Class II aaRS and biotin synthetases
81271 - TGS-like
55186 - ThrRS/AlaRS common domain

ModBase Predicted Comparative 3D Structure on A2RTX5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003674 molecular_function
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004829 threonine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016874 ligase activity

Biological Process:
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0008150 biological_process
GO:0043039 tRNA aminoacylation

Cellular Component:
GO:0005575 cellular_component
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AX721055 - Sequence 15 from Patent WO0220754.
AK057734 - Homo sapiens cDNA FLJ25005 fis, clone CBL00905.
AL833188 - Homo sapiens mRNA; cDNA DKFZp667K215 (from clone DKFZp667K215).
AK056653 - Homo sapiens cDNA FLJ32091 fis, clone OCBBF2000824, moderately similar to THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (EC 6.1.1.3).
BC035827 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone IMAGE:5582577), containing frame-shift errors.
BC132671 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone MGC:164302 IMAGE:40146693), complete cds.
BC136315 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone MGC:167925 IMAGE:9020302), complete cds.
BC143943 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone MGC:177478 IMAGE:9052461), complete cds.
BC069346 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone IMAGE:7262368), partial cds.
BC069811 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone IMAGE:7262285), partial cds.
BC074887 - Homo sapiens threonyl-tRNA synthetase-like 2, mRNA (cDNA clone IMAGE:30915243), partial cds.
KJ905604 - Synthetic construct Homo sapiens clone ccsbBroadEn_15234 TARSL2 gene, encodes complete protein.
JD038243 - Sequence 19267 from Patent EP1572962.
JD359983 - Sequence 341007 from Patent EP1572962.
JD361091 - Sequence 342115 from Patent EP1572962.
JD233929 - Sequence 214953 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
TRNA-CHARGING-PWY - tRNA charging

-  Other Names for This Gene
  Alternate Gene Symbols: A2RTX5, B2RMP7, ENST00000335968.1, ENST00000335968.2, ENST00000335968.3, ENST00000335968.4, ENST00000335968.5, ENST00000335968.6, ENST00000335968.7, NM_152334, Q6B0A1, Q6IS76, Q96LW3, Q96MP4, SYTC2_HUMAN, TARS3 , TARSL2, uc317ukg.1, uc317ukg.2
UCSC ID: ENST00000335968.8_9
RefSeq Accession: NM_152334.3
Protein: A2RTX5 (aka SYTC2_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.