Human Gene SPEF2 (ENST00000356031.8_8) from GENCODE V47lift37

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Description: sperm flagellar 2, transcript variant 1 (from RefSeq NM_024867.4)
Gencode Transcript: ENST00000356031.8_8
Gencode Gene: ENSG00000152582.14_15
Transcript (Including UTRs)
Position: hg19 chr5:35,617,965-35,814,713 Size: 196,749 Total Exon Count: 37 Strand: +
Coding Region
Position: hg19 chr5:35,618,100-35,814,655 Size: 196,556 Coding Exon Count: 37

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	mRNA Descriptions
Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr5:35,617,965-35,814,713)			mRNA (may differ from genome)		Protein (1822 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	UniProtKB	Wikipedia
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: SPEF2_HUMAN DESCRIPTION: RecName: Full=Sperm flagellar protein 2; AltName: Full=Protein KPL2; FUNCTION: Required for correct axoneme development (By similarity). SIMILARITY: Contains 1 CH (calponin-homology) domain. SEQUENCE CAUTION: Sequence=BAB15700.1; Type=Erroneous initiation;

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D000638 Amiodarone D000643 Ammonium Chloride D001564 Benzo(a)pyrene D019327 Copper Sulfate

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 4.77 RPKM in Cervix - Endocervix Total median expression: 56.01 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-54.40	135	-0.403	Picture	PostScript	Text
3' UTR	-2.30	58	-0.040	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR000850 - Adenylate_kin
IPR001715 - CH-domain
IPR010441 - DUF1042
IPR008906 - HATC

Pfam Domains:
PF00406 - Adenylate kinase
PF06294 - CH-like domain in sperm protein

SCOP Domains:
47473 - EF-hand
47576 - Calponin-homology domain, CH-domain
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on Q9C093


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD

Descriptions from all associated GenBank mRNAs


	BC067761 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:5298423), containing frame-shift errors. BC016773 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:4092589), with apparent retained intron. EF553528 - Homo sapiens clone DKFZp686K07130 FLJ23577 mRNA sequence, alternatively spliced. HM005670 - Homo sapiens clone HTL-T-47a testis tissue sperm-binding protein Li 47a mRNA, complete cds. AK058124 - Homo sapiens cDNA FLJ25395 fis, clone TST02553. BC110984 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:5163398), complete cds. BC156528 - Synthetic construct Homo sapiens clone IMAGE:100063160, MGC:190710 sperm flagellar 2 (SPEF2) mRNA, encodes complete protein. BC172520 - Synthetic construct Homo sapiens clone IMAGE:100069214, MGC:199225 sperm flagellar 2 (SPEF2) mRNA, encodes complete protein. AB051557 - Homo sapiens mRNA for KIAA1770 protein, partial cds. AK225586 - Homo sapiens mRNA for KPL2 protein isoform 1 variant, clone: LNG11288. AK027230 - Homo sapiens cDNA: FLJ23577 fis, clone LNG12640. AK225607 - Homo sapiens mRNA for KPL2 protein isoform 1 variant, clone: LNG12640. BX648691 - Homo sapiens mRNA; cDNA DKFZp686L2038 (from clone DKFZp686L2038). AK026817 - Homo sapiens cDNA: FLJ23164 fis, clone LNG09764. AL832607 - Homo sapiens mRNA; cDNA DKFZp451H1817 (from clone DKFZp451H1817). AK097547 - Homo sapiens cDNA FLJ40228 fis, clone TESTI2022623, moderately similar to Rattus norvegicus KPL2 (Kpl2) mRNA. AK094564 - Homo sapiens cDNA FLJ37245 fis, clone BRAMY2005488, moderately similar to Rattus norvegicus KPL2 (Kpl2) mRNA.

Other Names for This Gene


	Alternate Gene Symbols: ENST00000356031.1, ENST00000356031.2, ENST00000356031.3, ENST00000356031.4, ENST00000356031.5, ENST00000356031.6, ENST00000356031.7, KIAA1770, KPL2, NM_024867, Q2TAC9, Q96LL6, Q9C093, Q9H5C7, Q9H5Q7, SPEF2_HUMAN, uc317zjh.1, uc317zjh.2 UCSC ID: ENST00000356031.8_8 RefSeq Accession: NM_024867.4 Protein: Q9C093 (aka SPEF2_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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