Human Gene SPAG8 (ENST00000396638.7_10) from GENCODE V47lift37
  Description: sperm associated antigen 8, transcript variant 1 (from RefSeq NM_001039592.2)
Gencode Transcript: ENST00000396638.7_10
Gencode Gene: ENSG00000137098.14_15
Transcript (Including UTRs)
   Position: hg19 chr9:35,809,792-35,812,259 Size: 2,468 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr9:35,809,935-35,812,144 Size: 2,210 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:35,809,792-35,812,259)mRNA (may differ from genome)Protein (485 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SPAG8_HUMAN
DESCRIPTION: RecName: Full=Sperm-associated antigen 8; AltName: Full=HSD-1; AltName: Full=Sperm membrane protein 1; Short=SMP-1; AltName: Full=Sperm membrane protein BS-84;
FUNCTION: May play a role in fertility and microtubule formation through interaction with RANBP9.
SUBUNIT: Interacts with RANBP9.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Acrosomal region of the head of mature sperm cells.
TISSUE SPECIFICITY: Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.
SEQUENCE CAUTION: Sequence=AAB46833.1; Type=Erroneous termination; Positions=427; Note=Translated as stop;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.87 RPKM in Testis
Total median expression: 125.03 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -41.90115-0.364 Picture PostScript Text
3' UTR -42.60143-0.298 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026124 - Sperm-assoc_Ag8

ModBase Predicted Comparative 3D Structure on Q99932
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0005515 protein binding

Biological Process:
GO:0007049 cell cycle
GO:0007283 spermatogenesis
GO:0007338 single fertilization
GO:0008150 biological_process
GO:0030154 cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0001669 acrosomal vesicle
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005819 spindle
GO:0005856 cytoskeleton
GO:0016020 membrane
GO:0031410 cytoplasmic vesicle


-  Descriptions from all associated GenBank mRNAs
  BC019247 - Homo sapiens sperm associated antigen 8, mRNA (cDNA clone MGC:26201 IMAGE:4825175), complete cds.
S83157 - hSMP-1=sperm membrane protein [human, testis, mRNA, 2483 nt].
U12978 - Homo sapiens BS-84 (HSD-1) mRNA, complete cds.
KJ898607 - Synthetic construct Homo sapiens clone ccsbBroadEn_08001 SPAG8 gene, encodes complete protein.
DQ890798 - Synthetic construct clone IMAGE:100003428; FLH166019.01X; RZPDo839B1186D sperm associated antigen 8 (SPAG8) gene, encodes complete protein.
EU176503 - Synthetic construct Homo sapiens clone IMAGE:100011344; FLH166018.01L; RZPDo839F04253D sperm associated antigen 8 (SPAG8) gene, encodes complete protein.
HM005490 - Homo sapiens clone HTL-T-177 testicular tissue protein Li 177 mRNA, complete cds.
AK302313 - Homo sapiens cDNA FLJ60953 complete cds, highly similar to Homo sapiens sperm associated antigen 8 (SPAG8), transcript variant 1, mRNA.
JD296133 - Sequence 277157 from Patent EP1572962.
JD255495 - Sequence 236519 from Patent EP1572962.
CU676402 - Synthetic construct Homo sapiens gateway clone IMAGE:100019699 5' read SPAG8 mRNA.
JD541179 - Sequence 522203 from Patent EP1572962.
JD185103 - Sequence 166127 from Patent EP1572962.
JD274451 - Sequence 255475 from Patent EP1572962.
JD175080 - Sequence 156104 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B4DY89, E9PDV6, ENST00000396638.1, ENST00000396638.2, ENST00000396638.3, ENST00000396638.4, ENST00000396638.5, ENST00000396638.6, NM_001039592, Q12937, Q5TCV8, Q8WWB4, Q99932, SPAG8 , SPAG8_HUMAN, uc318yjr.1, uc318yjr.2
UCSC ID: ENST00000396638.7_10
RefSeq Accession: NM_001039592.2
Protein: Q99932 (aka SPAG8_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.