ID:PM14_HUMAN DESCRIPTION: RecName: Full=Pre-mRNA branch site protein p14; AltName: Full=SF3b 14 kDa subunit; FUNCTION: Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. SUBUNIT: Component of the splicing factor SF3b complex. Interacts with SF3B1/SF3b155. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. SUBCELLULAR LOCATION: Nucleus (Potential). SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Y3B4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF151868 - Homo sapiens CGI-110 protein mRNA, complete cds. BC015463 - Homo sapiens splicing factor 3B, 14 kDa subunit, mRNA (cDNA clone MGC:8723 IMAGE:3914184), complete cds. AK311118 - Homo sapiens cDNA, FLJ18160. AK000621 - Homo sapiens cDNA FLJ20614 fis, clone KAT05403, highly similar to AF151868 Homo sapiens CGI-110 protein mRNA. AF184213 - Homo sapiens ht006 protein mRNA, complete cds. AF161523 - Homo sapiens HSPC175 mRNA, complete cds. AK311908 - Homo sapiens cDNA, FLJ92174, Homo sapiens CGI-110 protein (CGI-110), mRNA. JF432474 - Synthetic construct Homo sapiens clone IMAGE:100073695 splicing factor 3B, 14 kDa subunit (SF3B14) gene, encodes complete protein. KJ893958 - Synthetic construct Homo sapiens clone ccsbBroadEn_03352 SF3B14 gene, encodes complete protein. AB527769 - Synthetic construct DNA, clone: pF1KB5825, Homo sapiens AC008073.5 gene for pre-mRNA branch site protein p14, without stop codon, in Flexi system. AF401310 - Homo sapiens pre-mRNA branch site protein p14 mRNA, complete cds. AF147425 - Homo sapiens full length insert cDNA clone YP78C06. JD510614 - Sequence 491638 from Patent EP1572962. JD310273 - Sequence 291297 from Patent EP1572962. DQ581903 - Homo sapiens piRNA piR-50015, complete sequence.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9Y3B4 (Reactome details) participates in the following event(s):
R-HSA-75080 Formation of AT-AC A complex R-HSA-75083 ATAC spliceosome mediated 3' splice site cleavage, exon ligation R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-75081 Formation of AT-AC B Complex R-HSA-75082 ATAC spliceosome mediated Lariat formation,5' splice site cleavage R-HSA-75079 Formation of AT-AC C complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72165 mRNA Splicing - Minor Pathway R-HSA-72172 mRNA Splicing R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-8953854 Metabolism of RNA
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
