ID:SF3A3_HUMAN DESCRIPTION: RecName: Full=Splicing factor 3A subunit 3; AltName: Full=SF3a60; AltName: Full=Spliceosome-associated protein 61; Short=SAP 61; FUNCTION: Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SUBUNIT: Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region. INTERACTION: Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-1051880, EBI-6248094; SUBCELLULAR LOCATION: Nucleus speckle. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the SF3A3 family. SIMILARITY: Contains 1 matrin-type zinc finger.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q12874
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK026887 - Homo sapiens cDNA: FLJ23234 fis, clone CAS03518. U08815 - Human splicesomal protein (SAP 61) mRNA, complete cds. X81789 - H.sapiens mRNA for splicing factor SF3a60. JD154709 - Sequence 135733 from Patent EP1572962. JD085914 - Sequence 66938 from Patent EP1572962. JD201633 - Sequence 182657 from Patent EP1572962. JD379947 - Sequence 360971 from Patent EP1572962. JD535368 - Sequence 516392 from Patent EP1572962. JD441473 - Sequence 422497 from Patent EP1572962. JD558364 - Sequence 539388 from Patent EP1572962. JD564462 - Sequence 545486 from Patent EP1572962. JD235901 - Sequence 216925 from Patent EP1572962. JD557511 - Sequence 538535 from Patent EP1572962. AK128438 - Homo sapiens cDNA FLJ46581 fis, clone THYMU3043200, highly similar to Splicing factor 3A subunit 3. JD059052 - Sequence 40076 from Patent EP1572962. JD105007 - Sequence 86031 from Patent EP1572962. JD078190 - Sequence 59214 from Patent EP1572962. JD472503 - Sequence 453527 from Patent EP1572962. JD327239 - Sequence 308263 from Patent EP1572962. JD167800 - Sequence 148824 from Patent EP1572962. JD493907 - Sequence 474931 from Patent EP1572962. AK292116 - Homo sapiens cDNA FLJ78053 complete cds, highly similar to Homo sapiens splicing factor 3a, subunit 3, 60kDa (SF3A3), mRNA. JD277740 - Sequence 258764 from Patent EP1572962. JD166403 - Sequence 147427 from Patent EP1572962. JD042161 - Sequence 23185 from Patent EP1572962. JD355072 - Sequence 336096 from Patent EP1572962. AK291181 - Homo sapiens cDNA FLJ78246 complete cds, highly similar to Homo sapiens splicing factor 3a, subunit 3, 60kDa (SF3A3), mRNA. AK222554 - Homo sapiens mRNA for splicing factor 3a, subunit 3 variant, clone: CAE04455. BC002395 - Homo sapiens splicing factor 3a, subunit 3, 60kDa, mRNA (cDNA clone MGC:8445 IMAGE:2821350), complete cds. BC011523 - Homo sapiens splicing factor 3a, subunit 3, 60kDa, mRNA (cDNA clone MGC:17701 IMAGE:3867442), complete cds. AK301424 - Homo sapiens cDNA FLJ58737 complete cds, highly similar to Splicing factor 3A subunit 3. JD039397 - Sequence 20421 from Patent EP1572962. JD093996 - Sequence 75020 from Patent EP1572962. DQ891120 - Synthetic construct clone IMAGE:100003750; FLH169486.01X; RZPDo839C1196D splicing factor 3a, subunit 3, 60kDa (SF3A3) gene, encodes complete protein. KJ898318 - Synthetic construct Homo sapiens clone ccsbBroadEn_07712 SF3A3 gene, encodes complete protein. EU176559 - Synthetic construct Homo sapiens clone IMAGE:100011376; FLH169485.01L; RZPDo839H10253D splicing factor 3a, subunit 3, 60kDa (SF3A3) gene, encodes complete protein. KJ906063 - Synthetic construct Homo sapiens clone ccsbBroadEn_15733 SF3A3 gene, encodes complete protein. AB464245 - Synthetic construct DNA, clone: pF1KB8175, Homo sapiens SF3A3 gene for splicing factor 3a, subunit 3, 60kDa, without stop codon, in Flexi system. CU677944 - Synthetic construct Homo sapiens gateway clone IMAGE:100017372 5' read SF3A3 mRNA.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q12874 (Reactome details) participates in the following event(s):
R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72172 mRNA Splicing R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-8953854 Metabolism of RNA
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
