ID:SELPL_HUMAN DESCRIPTION: RecName: Full=P-selectin glycoprotein ligand 1; Short=PSGL-1; AltName: Full=Selectin P ligand; AltName: CD_antigen=CD162; Flags: Precursor; FUNCTION: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. SUBUNIT: Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L- selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparantly not tyrosine sulfation. Dimerization appears not to be required for P- selectin/SELP binding. Interacts with SNX20. Interacts with MSN and SYK; mediates the activation of SYK by SELPLG. INTERACTION: Q7Z614:SNX20; NbExp=5; IntAct=EBI-1030190, EBI-744896; SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes. PTM: Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation. PTM: Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P- selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr- 48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling. WEB RESOURCE: Name=Wikipedia; Note=P-selectin glycoprotein ligand 1 entry; URL="http://en.wikipedia.org/wiki/P-selectin_glycoprotein_ligand-1"; WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/selplg/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q14242
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK295238 - Homo sapiens cDNA FLJ50962 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. AK098315 - Homo sapiens cDNA FLJ40996 fis, clone UTERU2015880, highly similar to P-SELECTIN GLYCOPROTEIN LIGAND 1 PRECURSOR. AK225644 - Homo sapiens mRNA for P-selectin glycoprotein ligand 1 precursor variant, clone: SPL06709. BC029782 - Homo sapiens selectin P ligand, mRNA (cDNA clone MGC:34401 IMAGE:5180824), complete cds. AK127638 - Homo sapiens cDNA FLJ45736 fis, clone JCMLC2002095, weakly similar to P-selectin glycoprotein ligand 1 precursor. JD495426 - Sequence 476450 from Patent EP1572962. AK298738 - Homo sapiens cDNA FLJ51042 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. JD289497 - Sequence 270521 from Patent EP1572962. JD486992 - Sequence 468016 from Patent EP1572962. JD293043 - Sequence 274067 from Patent EP1572962. U02297 - Human P-selectin ligand mRNA, complete cds. JD487078 - Sequence 468102 from Patent EP1572962. JD544820 - Sequence 525844 from Patent EP1572962. JD259666 - Sequence 240690 from Patent EP1572962. AK290395 - Homo sapiens cDNA FLJ78434 complete cds, highly similar to Homo sapiens selectin P ligand (SELPLG), mRNA. JD458141 - Sequence 439165 from Patent EP1572962. JD207014 - Sequence 188038 from Patent EP1572962. AK300057 - Homo sapiens cDNA FLJ52447 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. AK298742 - Homo sapiens cDNA FLJ53082 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. JD152750 - Sequence 133774 from Patent EP1572962. JD122870 - Sequence 103894 from Patent EP1572962. JD163977 - Sequence 145001 from Patent EP1572962. JD134591 - Sequence 115615 from Patent EP1572962. DQ892117 - Synthetic construct clone IMAGE:100004747; FLH183189.01X; RZPDo839G12140D selectin P ligand (SELPLG) gene, encodes complete protein. KJ892124 - Synthetic construct Homo sapiens clone ccsbBroadEn_01518 SELPLG gene, encodes complete protein. KR710699 - Synthetic construct Homo sapiens clone CCSBHm_00015677 SELPLG (SELPLG) mRNA, encodes complete protein. DQ895313 - Synthetic construct Homo sapiens clone IMAGE:100009773; FLH183185.01L; RZPDo839G12139D selectin P ligand (SELPLG) gene, encodes complete protein. AB464193 - Synthetic construct DNA, clone: pF1KB8995, Homo sapiens SELPLG gene for selectin P ligand, without stop codon, in Flexi system. CU688740 - Synthetic construct Homo sapiens gateway clone IMAGE:100019245 5' read SELPLG mRNA. JD056782 - Sequence 37806 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_LairPathway - Cells and Molecules involved in local acute inflammatory response h_granulocytesPathway - Adhesion and Diapedesis of Granulocytes
Reactome (by CSHL, EBI, and GO)
Protein Q14242 (Reactome details) participates in the following event(s):
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
