ID:RNF4_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase RNF4; EC=6.3.2.-; AltName: Full=RING finger protein 4; AltName: Full=Small nuclear ring finger protein; Short=Protein SNURF; FUNCTION: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Homodimer (via RING-type zinc finger domain). Interacts with GSC2 (By similarity). Interacts with AR/the androgen receptor and TBP (By similarity). Interacts with TCF20 (By similarity). Interacts with PATZ1. Interacts with TRPS1; negatively regulates TRPS1 transcriptional repressor activity. Interacts with PML; SUMO1-dependent. Interacts with PML; SUMO2-dependent. Interacts with PARP1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm. TISSUE SPECIFICITY: Widely expressed at low levels in many tissues; highly expressed in testis. DOMAIN: The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates (By similarity). PTM: Sumoylated; conjugated by one or two SUMO1 moieties (By similarity). PTM: Autoubiquitinated (By similarity). SIMILARITY: Contains 1 RING-type zinc finger.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P78317
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0016567 protein ubiquitination GO:0030521 androgen receptor signaling pathway GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process GO:0045893 positive regulation of transcription, DNA-templated GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0046685 response to arsenic-containing substance GO:0051865 protein autoubiquitination GO:0070534 protein K63-linked ubiquitination GO:0070936 protein K48-linked ubiquitination GO:0070979 protein K11-linked ubiquitination GO:0085020 protein K6-linked ubiquitination GO:0090169 regulation of spindle assembly GO:0090234 regulation of kinetochore assembly
AK307372 - Homo sapiens cDNA, FLJ97320. AB000468 - Homo sapiens mRNA for zinc finger protein, complete cds, clone:RES4-26. AK312534 - Homo sapiens cDNA, FLJ92903, Homo sapiens ring finger protein 4 (RNF4), mRNA. AK309509 - Homo sapiens cDNA, FLJ99550. U95140 - Homo sapiens RNF4 mRNA, complete cds. LF383961 - JP 2014500723-A/191464: Polycomb-Associated Non-Coding RNAs. BC031935 - Homo sapiens ring finger protein 4, mRNA (cDNA clone MGC:42534 IMAGE:4824114), complete cds. AK128038 - Homo sapiens cDNA FLJ46157 fis, clone TESTI4001679, highly similar to RING finger protein 4. MA619538 - JP 2018138019-A/191464: Polycomb-Associated Non-Coding RNAs. LF358188 - JP 2014500723-A/165691: Polycomb-Associated Non-Coding RNAs. MA593765 - JP 2018138019-A/165691: Polycomb-Associated Non-Coding RNAs. JD245460 - Sequence 226484 from Patent EP1572962. JD061744 - Sequence 42768 from Patent EP1572962. JD360575 - Sequence 341599 from Patent EP1572962. JD241453 - Sequence 222477 from Patent EP1572962. AB464178 - Synthetic construct DNA, clone: pF1KB8280, Homo sapiens RNF4 gene for ring finger protein 4, without stop codon, in Flexi system. EU446651 - Synthetic construct Homo sapiens clone IMAGE:100070216; IMAGE:100011860; FLH257295.01L ring finger protein 4 (RNF4) gene, encodes complete protein. AK311314 - Homo sapiens cDNA, FLJ18356. JD350232 - Sequence 331256 from Patent EP1572962. JD066602 - Sequence 47626 from Patent EP1572962. JD328552 - Sequence 309576 from Patent EP1572962. JD380454 - Sequence 361478 from Patent EP1572962. JD320936 - Sequence 301960 from Patent EP1572962. JD369011 - Sequence 350035 from Patent EP1572962. JD358657 - Sequence 339681 from Patent EP1572962. JD368912 - Sequence 349936 from Patent EP1572962. LF321566 - JP 2014500723-A/129069: Polycomb-Associated Non-Coding RNAs. JD385026 - Sequence 366050 from Patent EP1572962. JD390227 - Sequence 371251 from Patent EP1572962. JD402243 - Sequence 383267 from Patent EP1572962. JD041472 - Sequence 22496 from Patent EP1572962. JD081348 - Sequence 62372 from Patent EP1572962. JD207238 - Sequence 188262 from Patent EP1572962. JD108970 - Sequence 89994 from Patent EP1572962. JD408132 - Sequence 389156 from Patent EP1572962. JD215928 - Sequence 196952 from Patent EP1572962. JD209801 - Sequence 190825 from Patent EP1572962. JD456461 - Sequence 437485 from Patent EP1572962. JD460385 - Sequence 441409 from Patent EP1572962. JD150654 - Sequence 131678 from Patent EP1572962. JD232826 - Sequence 213850 from Patent EP1572962. JD494810 - Sequence 475834 from Patent EP1572962. JD086840 - Sequence 67864 from Patent EP1572962. JD296569 - Sequence 277593 from Patent EP1572962. JD293049 - Sequence 274073 from Patent EP1572962. JD546589 - Sequence 527613 from Patent EP1572962. MA557143 - JP 2018138019-A/129069: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P78317 (Reactome details) participates in the following event(s):
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-983147 Release of E3 from polyubiquitinated substrate R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-5684071 RNF4 ubiquitinates MDC1 R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-5693607 Processing of DNA double-strand break ends R-HSA-1280218 Adaptive Immune System R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-168256 Immune System R-HSA-5693538 Homology Directed Repair R-HSA-5693532 DNA Double-Strand Break Repair R-HSA-73894 DNA Repair
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
