Human Gene RLN3 (ENST00000431365.3_5) from GENCODE V47lift37
  Description: relaxin 3, transcript variant 1 (from RefSeq NM_080864.4)
Gencode Transcript: ENST00000431365.3_5
Gencode Gene: ENSG00000171136.7_8
Transcript (Including UTRs)
   Position: hg19 chr19:14,138,960-14,142,363 Size: 3,404 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr19:14,139,017-14,141,760 Size: 2,744 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:14,138,960-14,142,363)mRNA (may differ from genome)Protein (142 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: REL3_HUMAN
DESCRIPTION: RecName: Full=Relaxin-3; AltName: Full=Insulin-like peptide INSL7; Short=Insulin-like peptide 7; AltName: Full=Prorelaxin H3; Contains: RecName: Full=Relaxin-3 B chain; Contains: RecName: Full=Relaxin-3 A chain; Flags: Precursor;
FUNCTION: May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the insulin family.
SEQUENCE CAUTION: Sequence=AAQ88548.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.04 RPKM in Testis
Total median expression: 2.00 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.7057-0.153 Picture PostScript Text
3' UTR -176.00603-0.292 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016179 - Insulin-like
IPR022353 - Insulin_CS
IPR022352 - Insulin_family

Pfam Domains:
PF00049 - Insulin/IGF/Relaxin family

SCOP Domains:
56994 - Insulin-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2FHW - NMR 2K1V - NMR


ModBase Predicted Comparative 3D Structure on Q8WXF3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001664 G-protein coupled receptor binding
GO:0005179 hormone activity
GO:0005515 protein binding

Biological Process:
GO:0007186 G-protein coupled receptor signaling pathway
GO:0010469 regulation of receptor activity

Cellular Component:
GO:0005576 extracellular region


-  Descriptions from all associated GenBank mRNAs
  AY358181 - Homo sapiens clone DNA171372 RLN3 (UNQ6188) mRNA, complete cds.
BC140935 - Homo sapiens relaxin 3, mRNA (cDNA clone MGC:176612 IMAGE:9056747), complete cds.
KJ904968 - Synthetic construct Homo sapiens clone ccsbBroadEn_14362 RLN3 gene, encodes complete protein.
AF447451 - Homo sapiens relaxin 3 (RLN3) mRNA, complete cds.
BC110069 - Homo sapiens relaxin 3, mRNA (cDNA clone IMAGE:40014224).
AB076563 - Homo sapiens insl7 mRNA for insulin-like 7, complete cds.
JD045218 - Sequence 26242 from Patent EP1572962.
JD282564 - Sequence 263588 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8WXF3 (Reactome details) participates in the following event(s):

R-HSA-444848 Relaxin 3 receptor 1 binds relaxin 3
R-HSA-444838 Relaxin receptor 1 binds relaxin 2 and 3
R-HSA-444859 Relaxin 3 receptor 2 binds relaxin 3 and INSL5
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-744886 The Ligand:GPCR:Gs complex dissociates
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-744887 Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
R-HSA-379044 Liganded Gs-activating GPCR acts as a GEF for Gs
R-HSA-444821 Relaxin receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-418555 G alpha (s) signalling events
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000431365.1, ENST00000431365.2, INSL7, NM_080864, Q6UXW5, Q8WXF3, REL3_HUMAN, RXN3, uc320acv.1, uc320acv.2, UNQ6188/PRO20213, ZINS4
UCSC ID: ENST00000431365.3_5
RefSeq Accession: NM_080864.4
Protein: Q8WXF3 (aka REL3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.