ID:RAD1_HUMAN DESCRIPTION: RecName: Full=Cell cycle checkpoint protein RAD1; Short=hRAD1; EC=3.1.11.2; AltName: Full=DNA repair exonuclease rad1 homolog; AltName: Full=Rad1-like DNA damage checkpoint protein; FUNCTION: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates. SUBUNIT: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7. INTERACTION: O60921:HUS1; NbExp=4; IntAct=EBI-721835, EBI-1056174; Q99638:RAD9A; NbExp=2; IntAct=EBI-721835, EBI-2606224; SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). SIMILARITY: Belongs to the rad1 family. SEQUENCE CAUTION: Sequence=AAC35550.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAC35550.1; Type=Frameshift; Positions=103; Sequence=CAA06249.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAA06249.1; Type=Frameshift; Positions=103; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rad1/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60671
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000077 DNA damage checkpoint GO:0006281 DNA repair GO:0006974 cellular response to DNA damage stimulus GO:0021762 substantia nigra development GO:0051598 meiotic recombination checkpoint GO:0071479 cellular response to ionizing radiation GO:0090305 nucleic acid phosphodiester bond hydrolysis
LF210228 - JP 2014500723-A/17731: Polycomb-Associated Non-Coding RNAs. BC035771 - Homo sapiens RAD1 homolog (S. pombe), mRNA (cDNA clone IMAGE:5582524), with apparent retained intron. AK096960 - Homo sapiens cDNA FLJ39641 fis, clone SMINT2003423. JD414700 - Sequence 395724 from Patent EP1572962. AF030933 - Homo sapiens exonuclease homolog RAD1 (RAD1) mRNA, complete cds. BC006837 - Homo sapiens RAD1 homolog (S. pombe), mRNA (cDNA clone MGC:5110 IMAGE:3450321), complete cds. JD125717 - Sequence 106741 from Patent EP1572962. JD055243 - Sequence 36267 from Patent EP1572962. JD306380 - Sequence 287404 from Patent EP1572962. JD125716 - Sequence 106740 from Patent EP1572962. JD055242 - Sequence 36266 from Patent EP1572962. JD306379 - Sequence 287403 from Patent EP1572962. JD169727 - Sequence 150751 from Patent EP1572962. JD135983 - Sequence 117007 from Patent EP1572962. JD233041 - Sequence 214065 from Patent EP1572962. JD052747 - Sequence 33771 from Patent EP1572962. JD451312 - Sequence 432336 from Patent EP1572962. JD473595 - Sequence 454619 from Patent EP1572962. JD194775 - Sequence 175799 from Patent EP1572962. JD274709 - Sequence 255733 from Patent EP1572962. JD194774 - Sequence 175798 from Patent EP1572962. JD144554 - Sequence 125578 from Patent EP1572962. JD394621 - Sequence 375645 from Patent EP1572962. JD410076 - Sequence 391100 from Patent EP1572962. JD410075 - Sequence 391099 from Patent EP1572962. JD438897 - Sequence 419921 from Patent EP1572962. JD264179 - Sequence 245203 from Patent EP1572962. JD105345 - Sequence 86369 from Patent EP1572962. JD036732 - Sequence 17756 from Patent EP1572962. JD329105 - Sequence 310129 from Patent EP1572962. JD434361 - Sequence 415385 from Patent EP1572962. JD043108 - Sequence 24132 from Patent EP1572962. JD420224 - Sequence 401248 from Patent EP1572962. JD420225 - Sequence 401249 from Patent EP1572962. JD329106 - Sequence 310130 from Patent EP1572962. JD441388 - Sequence 422412 from Patent EP1572962. JD564332 - Sequence 545356 from Patent EP1572962. JD441387 - Sequence 422411 from Patent EP1572962. JD556339 - Sequence 537363 from Patent EP1572962. JD319314 - Sequence 300338 from Patent EP1572962. JD340831 - Sequence 321855 from Patent EP1572962. JD171558 - Sequence 152582 from Patent EP1572962. JD238119 - Sequence 219143 from Patent EP1572962. JD279117 - Sequence 260141 from Patent EP1572962. JD231946 - Sequence 212970 from Patent EP1572962. JD283409 - Sequence 264433 from Patent EP1572962. JD233300 - Sequence 214324 from Patent EP1572962. JD223824 - Sequence 204848 from Patent EP1572962. JD348991 - Sequence 330015 from Patent EP1572962. AF011905 - Homo sapiens putative checkpoint control protein HRAD1 mRNA, complete cds. AF076841 - Homo sapiens Rad1-like protein (RAD1) mRNA, complete cds. AK002112 - Homo sapiens cDNA FLJ11250 fis, clone PLACE1008808, highly similar to Cell cycle checkpoint protein RAD1 (EC 3.1.11.2). BC009804 - Homo sapiens RAD1 homolog (S. pombe), mRNA (cDNA clone MGC:13690 IMAGE:4109436), complete cds. AJ004974 - Homo sapiens mRNA for cell cycle checkpoint protein rad1A. AJ004975 - Homo sapiens mRNA for cell cycle checkpoint protein rad1B. AF084513 - Homo sapiens DNA repair exonuclease (REC1) mRNA, alternatively spliced product, complete cds. AF073524 - Homo sapiens RAD1 mRNA, complete cds. AF090170 - Homo sapiens Rad1-like protein (RAD1) mRNA, complete cds. AF084512 - Homo sapiens DNA repair exonuclease (REC1) mRNA, complete cds. JD502941 - Sequence 483965 from Patent EP1572962. BC037857 - Homo sapiens RAD1 homolog (S. pombe), mRNA (cDNA clone MGC:43773 IMAGE:5272408), complete cds. LF336011 - JP 2014500723-A/143514: Polycomb-Associated Non-Coding RNAs. JD422103 - Sequence 403127 from Patent EP1572962. JD348419 - Sequence 329443 from Patent EP1572962. AF074717 - Homo sapiens Rad1-like DNA damage checkpoint protein (RAD1) mRNA, complete cds. JD080595 - Sequence 61619 from Patent EP1572962. AF058392 - Homo sapiens cell cycle checkpoint protein Hrad1 mRNA, complete cds. AF058393 - Homo sapiens cell cycle checkpoint protein Hrad1 mRNA, alternatively spliced. KJ891953 - Synthetic construct Homo sapiens clone ccsbBroadEn_01347 RAD1 gene, encodes complete protein. BT006908 - Homo sapiens RAD1 homolog (S. pombe) mRNA, complete cds. CU675471 - Synthetic construct Homo sapiens gateway clone IMAGE:100018718 5' read RAD1 mRNA. DQ891144 - Synthetic construct clone IMAGE:100003774; FLH169712.01X; RZPDo839F0496D RAD1 homolog (S. pombe) (RAD1) gene, encodes complete protein. DQ894324 - Synthetic construct Homo sapiens clone IMAGE:100008784; FLH169708.01L; RZPDo839F0495D RAD1 homolog (S. pombe) (RAD1) gene, encodes complete protein. AB528352 - Synthetic construct DNA, clone: pF1KB6324, Homo sapiens RAD1 gene for RAD1 homolog, without stop codon, in Flexi system. LF336008 - JP 2014500723-A/143511: Polycomb-Associated Non-Coding RNAs. JD027170 - Sequence 8194 from Patent EP1572962. JD028455 - Sequence 9479 from Patent EP1572962. JD023302 - Sequence 4326 from Patent EP1572962. JD033473 - Sequence 14497 from Patent EP1572962. JD177309 - Sequence 158333 from Patent EP1572962. DL491683 - Novel nucleic acids. JD193159 - Sequence 174183 from Patent EP1572962. JD069275 - Sequence 50299 from Patent EP1572962. JD443479 - Sequence 424503 from Patent EP1572962. DL490267 - Novel nucleic acids. MA445805 - JP 2018138019-A/17731: Polycomb-Associated Non-Coding RNAs. MA571588 - JP 2018138019-A/143514: Polycomb-Associated Non-Coding RNAs. MA571585 - JP 2018138019-A/143511: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O60671 (Reactome details) participates in the following event(s):
R-HSA-176264 Recruitment of the Rad9-Hus1-Rad1 complex to DNA R-HSA-5685011 ATR activation at DNA DSBs R-HSA-5686657 ERCC1:XPF cleaves flaps generated by SSA R-HSA-5693561 RAD51 binds BRCA2 at resected DNA DSBs R-HSA-5684887 Activation of CHEK1 at resected DNA DSBs R-HSA-5684882 CHEK1 is recruited to resected DNA DSBs R-HSA-5685156 ATR phosphorylates RPA2 R-HSA-5693580 Association of RAD52 with the RPA complex at resected DNA DSBs R-HSA-6799332 ATR phosphorylates TP53 R-HSA-5693564 Association of RAD51 with RAD52:DNA double-strand break ends R-HSA-5686642 RAD52 promotes single strand annealing at resected DNA DSBs R-HSA-176187 Activation of ATR in response to replication stress R-HSA-5693607 Processing of DNA double-strand break ends R-HSA-5685938 HDR through Single Strand Annealing (SSA) R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange R-HSA-69481 G2/M Checkpoints R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-5693579 Homologous DNA Pairing and Strand Exchange R-HSA-69473 G2/M DNA damage checkpoint R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-69620 Cell Cycle Checkpoints R-HSA-5693538 Homology Directed Repair R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-5633007 Regulation of TP53 Activity R-HSA-1640170 Cell Cycle R-HSA-5693532 DNA Double-Strand Break Repair R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-73894 DNA Repair R-HSA-212436 Generic Transcription Pathway R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
