ID:RGAP1_HUMAN DESCRIPTION: RecName: Full=Rac GTPase-activating protein 1; AltName: Full=Male germ cell RacGap; Short=MgcRacGAP; AltName: Full=Protein CYK4 homolg; Short=CYK4; Short=HsCYK-4; FUNCTION: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. SUBUNIT: Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Associates with alpha-, beta- and gamma-tubulin and microtubules. Interacts via its Rho-GAP domain with RND2. Associates with AURKB during M phase. Interacts via its Rho-GAP domain and basic region with PRC1. The interaction with PRC1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts with SLC26A8 via its N-terminus. Interacts with RAB11FIP3. Interacts with ECT2; the interaction is direct, occurs at anaphase and during cytokinesis in a microtubule-dependent manner and is enhanced by phosphorylation by PLK1. Interacts with KIF23; the interaction is direct. INTERACTION: Q96RN1:SLC26A8; NbExp=2; IntAct=EBI-717233, EBI-1792052; SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody. Note=Colocalizes with RND2 in Golgi- derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. TISSUE SPECIFICITY: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase. INDUCTION: Expression is down-regulated during macrophage differention of HL-60 cells. DOMAIN: The coiled coil region is indispensible for localization to the midbody during cytokinesis. PTM: Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation. SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger. SIMILARITY: Contains 1 Rho-GAP domain. SEQUENCE CAUTION: Sequence=AAH24144.1; Type=Frameshift; Positions=171, 205, 437;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9H0H5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000281 mitotic cytokinesis GO:0000915 actomyosin contractile ring assembly GO:0006811 ion transport GO:0006890 retrograde vesicle-mediated transport, Golgi to ER GO:0007018 microtubule-based movement GO:0007049 cell cycle GO:0007165 signal transduction GO:0007275 multicellular organism development GO:0007283 spermatogenesis GO:0007405 neuroblast proliferation GO:0008272 sulfate transport GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0030154 cell differentiation GO:0032467 positive regulation of cytokinesis GO:0035556 intracellular signal transduction GO:0043547 positive regulation of GTPase activity GO:0045995 regulation of embryonic development GO:0051056 regulation of small GTPase mediated signal transduction GO:0051256 mitotic spindle midzone assembly GO:0051301 cell division GO:0051988 regulation of attachment of spindle microtubules to kinetochore
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
