Human Gene PPP1R1B (ENST00000254079.9_4) from GENCODE V47lift37

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Description: protein phosphatase 1 regulatory inhibitor subunit 1B, transcript variant 1 (from RefSeq NM_032192.4)
Gencode Transcript: ENST00000254079.9_4
Gencode Gene: ENSG00000131771.14_9
Transcript (Including UTRs)
Position: hg19 chr17:37,783,205-37,792,877 Size: 9,673 Total Exon Count: 7 Strand: +
Coding Region
Position: hg19 chr17:37,783,646-37,792,118 Size: 8,473 Coding Exon Count: 7

Page Index	Sequence and Links	UniProtKB Comments	Primers	MalaCards	CTD
Gene Alleles	RNA-Seq Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr17:37,783,205-37,792,877)			mRNA (may differ from genome)		Protein (204 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	Malacards	MGI	OMIM	PubMed	Reactome
UniProtKB	Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: PPR1B_HUMAN DESCRIPTION: RecName: Full=Protein phosphatase 1 regulatory subunit 1B; AltName: Full=DARPP-32; AltName: Full=Dopamine- and cAMP-regulated neuronal phosphoprotein; FUNCTION: Inhibitor of protein-phosphatase 1. SUBCELLULAR LOCATION: Cytoplasm. PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. PTM: Phosphorylation of Thr-34 is required for activity (By similarity). SIMILARITY: Belongs to the protein phosphatase inhibitor 1 family. WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org//Genes/PPP1R1BID44096ch17q12.html";

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

MalaCards Disease Associations


	MalaCards Gene Search: PPP1R1B Diseases sorted by gene-association score: schizophrenia (2), attention deficit-hyperactivity disorder (2)

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D001564 Benzo(a)pyrene D003042 Cocaine D004958 Estradiol D007980 Levodopa D009020 Morphine D016627 Oxidopamine D020891 Raclopride C067113 SK&F 81297 C104586 roscovitine C060229 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione D015632 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine C087141 2-(1-(4-piperonyl)piperazinyl)benzothiazole C085911 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one C406233 2-ethyl-5-methoxy-N,N-dimethyltryptamine C072790 2-methoxy-4-amino-5-chlorobenzoic acid 2-(diethylamino)ethyl ester C054649 3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol C083009 4-amino-1-(6-chloro-2-pyridyl)piperidine hydrochloride C015952 4-iodo-2,5-dimethoxyphenylisopropylamine D008735 5-Methoxytryptamine D018750 6-Cyano-7-nitroquinoxaline-2,3-dione C121465 6-methyl-2-(phenylethynyl)pyridine C547126 AZM551248 D000082 Acetaminophen D000324 Adrenocorticotropic Hormone D002087 Butyrates D002166 Camptothecin D002518 Ceramides D002945 Cisplatin D003024 Clozapine D003345 Corticosterone D016291 Dizocilpine Maleate D004298 Dopamine D004317 Doxorubicin D000431 Ethanol D004997 Ethinyl Estradiol D005472 Fluorouracil D005473 Fluoxetine D005576 Forskolin D019833 Genistein D005839 Gentamicins D006220 Haloperidol D007501 Iron D007649 Ketamine D007650 Ketanserin D008345 Manganese D008694 Methamphetamine D008727 Methotrexate D052998 Microcystins C478058 N,N'-dicyclopentyl-2-methylsulfanyl-5-nitro-pyrimidine-4,6-diamine D011374 Progesterone D019257 Quinpirole C114163 Ro 4-6790 C534628 SCH 23390 C071262 SK&F 82958 C114947 SR 142948 D012701 Serotonin D020123 Sirolimus D013749 Tetrachlorodibenzodioxin D013779 Tetrodotoxin D014028 Tobacco Smoke Pollution D014212 Tretinoin D014635 Valproic Acid D014640 Vancomycin D014750 Vincristine C059765 amphotericin B, deoxycholate drug combination C075631 anpirtoline C005177 benserazide, levodopa drug combination C006780 bisphenol A C018021 cobaltous chloride C107676 deguelin C434247 diphenylditelluride C045989 eticlopride C016517 indole-3-carbinol C034643 naloxonazine D010133 p-Chloroamphetamine C006253 pirinixic acid C009495 titanium dioxide C009687 wortmannin

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 794.34 RPKM in Brain - Caudate (basal ganglia) Total median expression: 3214.46 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-203.90	441	-0.462	Picture	PostScript	Text
3' UTR	-266.20	759	-0.351	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR015670 - Dopamine/cAMP_neuron_P-prot
IPR008466 - PPI_1DARPP-32

Pfam Domains:
PF05395 - Protein phosphatase inhibitor 1/DARPP-32

ModBase Predicted Comparative 3D Structure on Q9UD71


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD	Ensembl

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0004860 protein kinase inhibitor activity GO:0004862 cAMP-dependent protein kinase inhibitor activity GO:0004864 protein phosphatase inhibitor activity GO:0004865 protein serine/threonine phosphatase inhibitor activity GO:0005515 protein binding GO:0019888 protein phosphatase regulator activity GO:0031748 D1 dopamine receptor binding GO:0031749 D2 dopamine receptor binding GO:0031750 D3 dopamine receptor binding GO:0031751 D4 dopamine receptor binding GO:0031752 D5 dopamine receptor binding Biological Process: GO:0001975 response to amphetamine GO:0006351 transcription, DNA-templated GO:0006469 negative regulation of protein kinase activity GO:0007165 signal transduction GO:0007621 negative regulation of female receptivity GO:0007626 locomotory behavior GO:0008542 visual learning GO:0032515 negative regulation of phosphoprotein phosphatase activity GO:0035556 intracellular signal transduction GO:0042220 response to cocaine GO:0043278 response to morphine GO:0043987 histone H3-S10 phosphorylation GO:0048148 behavioral response to cocaine GO:0071901 negative regulation of protein serine/threonine kinase activity Cellular Component: GO:0005634 nucleus GO:0005737 cytoplasm GO:0005829 cytosol GO:0043025 neuronal cell body

Descriptions from all associated GenBank mRNAs


	LF212448 - JP 2014500723-A/19951: Polycomb-Associated Non-Coding RNAs. AF464196 - Homo sapiens dopamine and cAMP-regulated neuronal phosphoprotein (DARPP32) mRNA, complete cds. JD394728 - Sequence 375752 from Patent EP1572962. JD532351 - Sequence 513375 from Patent EP1572962. JD336042 - Sequence 317066 from Patent EP1572962. AK127542 - Homo sapiens cDNA FLJ45635 fis, clone COLON2001829, highly similar to Dopamine- and cAMP-regulated neuronal phosphoprotein. AK123112 - Homo sapiens cDNA FLJ41117 fis, clone BRACE2006821, highly similar to Dopamine- and cAMP-regulated neuronalphospho protein. AK129537 - Homo sapiens cDNA FLJ26026 fis, clone PNC01609, highly similar to DOPAMINE- AND CAMP-REGULATED NEURONAL PHOSPHOPROTEIN. JD437580 - Sequence 418604 from Patent EP1572962. JD100592 - Sequence 81616 from Patent EP1572962. AF435972 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform A (PPP1R1B) mRNA, complete cds; alternatively spliced. JD057371 - Sequence 38395 from Patent EP1572962. JD175221 - Sequence 156245 from Patent EP1572962. JD445540 - Sequence 426564 from Patent EP1572962. JD343285 - Sequence 324309 from Patent EP1572962. JD446689 - Sequence 427713 from Patent EP1572962. JD330732 - Sequence 311756 from Patent EP1572962. JD118947 - Sequence 99971 from Patent EP1572962. JD460764 - Sequence 441788 from Patent EP1572962. JD192383 - Sequence 173407 from Patent EP1572962. JD538018 - Sequence 519042 from Patent EP1572962. JD458644 - Sequence 439668 from Patent EP1572962. JD129019 - Sequence 110043 from Patent EP1572962. JD387393 - Sequence 368417 from Patent EP1572962. AY070271 - Homo sapiens truncated DARPP-32 isoform t-DARPP (PPP1R1B) mRNA, complete cds, alternatively spliced. AF435973 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform B (PPP1R1B) mRNA, complete cds; alternatively spliced transcript B1. AF435974 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform B (PPP1R1B) mRNA, complete cds; alternatively spliced transcript B2. AK024593 - Homo sapiens cDNA: FLJ20940 fis, clone ADSE01608. AK225058 - Homo sapiens mRNA for protein phosphatase 1, regulatory (inhibitor) subunit 1B isoform 1 variant, clone: adSE01608. BC001519 - Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B, mRNA (cDNA clone MGC:2855 IMAGE:2987943), complete cds. CU674748 - Synthetic construct Homo sapiens gateway clone IMAGE:100016733 5' read PPP1R1B mRNA. KJ894942 - Synthetic construct Homo sapiens clone ccsbBroadEn_04336 PPP1R1B gene, encodes complete protein. BT007076 - Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32) mRNA, complete cds. BX648641 - Homo sapiens mRNA; cDNA DKFZp686C19219 (from clone DKFZp686C19219). AK123950 - Homo sapiens cDNA FLJ41956 fis, clone PROST2019781. AK124417 - Homo sapiens cDNA FLJ42426 fis, clone BLADE2004389. AF233349 - Homo sapiens neuronal phosphoprotein DARPP-32 mRNA, partial cds. JD532461 - Sequence 513485 from Patent EP1572962. JD507973 - Sequence 488997 from Patent EP1572962. JD156574 - Sequence 137598 from Patent EP1572962. JD214980 - Sequence 196004 from Patent EP1572962. JD417980 - Sequence 399004 from Patent EP1572962. JD193719 - Sequence 174743 from Patent EP1572962. JD149953 - Sequence 130977 from Patent EP1572962. JD251698 - Sequence 232722 from Patent EP1572962. JD387713 - Sequence 368737 from Patent EP1572962. JD462907 - Sequence 443931 from Patent EP1572962. JD548994 - Sequence 530018 from Patent EP1572962. JD208055 - Sequence 189079 from Patent EP1572962. JD357808 - Sequence 338832 from Patent EP1572962. JD019931 - Sequence 955 from Patent EP1572962. JD033308 - Sequence 14332 from Patent EP1572962. JD371886 - Sequence 352910 from Patent EP1572962. JD555057 - Sequence 536081 from Patent EP1572962. JD420589 - Sequence 401613 from Patent EP1572962. MA448025 - JP 2018138019-A/19951: Polycomb-Associated Non-Coding RNAs.

Biochemical and Signaling Pathways


	BioCarta from NCI Cancer Genome Anatomy Project h_fosbPathway - FOSB gene expression and drug abuse h_ck1Pathway - Regulation of ck1/cdk5 by type 1 glutamate receptors Reactome (by CSHL, EBI, and GO) Protein Q9UD71 (Reactome details) participates in the following event(s): R-HSA-180047 CDK5 phosphorylates DARPP-32 on Thr75 R-HSA-201790 DARPP-32 is dephosphorylated on Thr75 by PP2A R-HSA-180038 DARPP-32 phosphorylated on T34 binds to PP1, inhibiting its function R-HSA-201787 PPP3 complex dephosphorylates DARPP-32 on Thr34 R-HSA-177275 PKA phosphorylates DARPP-32 on Thr34 R-HSA-180073 DARPP-32 phosphorylated on Thr75 binds to PKA, inhibiting its function R-HSA-180024 DARPP-32 events R-HSA-111885 Opioid Signalling R-HSA-418594 G alpha (i) signalling events R-HSA-388396 GPCR downstream signalling R-HSA-372790 Signaling by GPCR R-HSA-162582 Signal Transduction

Other Names for This Gene


	Alternate Gene Symbols: DARPP32, ENST00000254079.1, ENST00000254079.2, ENST00000254079.3, ENST00000254079.4, ENST00000254079.5, ENST00000254079.6, ENST00000254079.7, ENST00000254079.8, NM_032192, PPR1B_HUMAN, Q547V9, Q547W0, Q9H7G1, Q9UD71, uc317fnd.1, uc317fnd.2 UCSC ID: ENST00000254079.9_4 RefSeq Accession: NM_032192.4 Protein: Q9UD71 (aka PPR1B_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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