Human Gene PPP1R1B (ENST00000254079.9_4) from GENCODE V47lift37
  Description: protein phosphatase 1 regulatory inhibitor subunit 1B, transcript variant 1 (from RefSeq NM_032192.4)
Gencode Transcript: ENST00000254079.9_4
Gencode Gene: ENSG00000131771.14_9
Transcript (Including UTRs)
   Position: hg19 chr17:37,783,205-37,792,877 Size: 9,673 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr17:37,783,646-37,792,118 Size: 8,473 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:37,783,205-37,792,877)mRNA (may differ from genome)Protein (204 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PPR1B_HUMAN
DESCRIPTION: RecName: Full=Protein phosphatase 1 regulatory subunit 1B; AltName: Full=DARPP-32; AltName: Full=Dopamine- and cAMP-regulated neuronal phosphoprotein;
FUNCTION: Inhibitor of protein-phosphatase 1.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
PTM: Phosphorylation of Thr-34 is required for activity (By similarity).
SIMILARITY: Belongs to the protein phosphatase inhibitor 1 family.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org//Genes/PPP1R1BID44096ch17q12.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PPP1R1B
Diseases sorted by gene-association score: schizophrenia (2), attention deficit-hyperactivity disorder (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 794.34 RPKM in Brain - Caudate (basal ganglia)
Total median expression: 3214.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -203.90441-0.462 Picture PostScript Text
3' UTR -266.20759-0.351 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015670 - Dopamine/cAMP_neuron_P-prot
IPR008466 - PPI_1DARPP-32

Pfam Domains:
PF05395 - Protein phosphatase inhibitor 1/DARPP-32

ModBase Predicted Comparative 3D Structure on Q9UD71
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004860 protein kinase inhibitor activity
GO:0004862 cAMP-dependent protein kinase inhibitor activity
GO:0004864 protein phosphatase inhibitor activity
GO:0004865 protein serine/threonine phosphatase inhibitor activity
GO:0005515 protein binding
GO:0019888 protein phosphatase regulator activity
GO:0031748 D1 dopamine receptor binding
GO:0031749 D2 dopamine receptor binding
GO:0031750 D3 dopamine receptor binding
GO:0031751 D4 dopamine receptor binding
GO:0031752 D5 dopamine receptor binding

Biological Process:
GO:0001975 response to amphetamine
GO:0006351 transcription, DNA-templated
GO:0006469 negative regulation of protein kinase activity
GO:0007165 signal transduction
GO:0007621 negative regulation of female receptivity
GO:0007626 locomotory behavior
GO:0008542 visual learning
GO:0032515 negative regulation of phosphoprotein phosphatase activity
GO:0035556 intracellular signal transduction
GO:0042220 response to cocaine
GO:0043278 response to morphine
GO:0043987 histone H3-S10 phosphorylation
GO:0048148 behavioral response to cocaine
GO:0071901 negative regulation of protein serine/threonine kinase activity

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0043025 neuronal cell body


-  Descriptions from all associated GenBank mRNAs
  LF212448 - JP 2014500723-A/19951: Polycomb-Associated Non-Coding RNAs.
AF464196 - Homo sapiens dopamine and cAMP-regulated neuronal phosphoprotein (DARPP32) mRNA, complete cds.
JD394728 - Sequence 375752 from Patent EP1572962.
JD532351 - Sequence 513375 from Patent EP1572962.
JD336042 - Sequence 317066 from Patent EP1572962.
AK127542 - Homo sapiens cDNA FLJ45635 fis, clone COLON2001829, highly similar to Dopamine- and cAMP-regulated neuronal phosphoprotein.
AK123112 - Homo sapiens cDNA FLJ41117 fis, clone BRACE2006821, highly similar to Dopamine- and cAMP-regulated neuronalphospho protein.
AK129537 - Homo sapiens cDNA FLJ26026 fis, clone PNC01609, highly similar to DOPAMINE- AND CAMP-REGULATED NEURONAL PHOSPHOPROTEIN.
JD437580 - Sequence 418604 from Patent EP1572962.
JD100592 - Sequence 81616 from Patent EP1572962.
AF435972 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform A (PPP1R1B) mRNA, complete cds; alternatively spliced.
JD057371 - Sequence 38395 from Patent EP1572962.
JD175221 - Sequence 156245 from Patent EP1572962.
JD445540 - Sequence 426564 from Patent EP1572962.
JD343285 - Sequence 324309 from Patent EP1572962.
JD446689 - Sequence 427713 from Patent EP1572962.
JD330732 - Sequence 311756 from Patent EP1572962.
JD118947 - Sequence 99971 from Patent EP1572962.
JD460764 - Sequence 441788 from Patent EP1572962.
JD192383 - Sequence 173407 from Patent EP1572962.
JD538018 - Sequence 519042 from Patent EP1572962.
JD458644 - Sequence 439668 from Patent EP1572962.
JD129019 - Sequence 110043 from Patent EP1572962.
JD387393 - Sequence 368417 from Patent EP1572962.
AY070271 - Homo sapiens truncated DARPP-32 isoform t-DARPP (PPP1R1B) mRNA, complete cds, alternatively spliced.
AF435973 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform B (PPP1R1B) mRNA, complete cds; alternatively spliced transcript B1.
AF435974 - Homo sapiens dopamine- and cAMP-regulated neuronal phosphoprotein isoform B (PPP1R1B) mRNA, complete cds; alternatively spliced transcript B2.
AK024593 - Homo sapiens cDNA: FLJ20940 fis, clone ADSE01608.
AK225058 - Homo sapiens mRNA for protein phosphatase 1, regulatory (inhibitor) subunit 1B isoform 1 variant, clone: adSE01608.
BC001519 - Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B, mRNA (cDNA clone MGC:2855 IMAGE:2987943), complete cds.
CU674748 - Synthetic construct Homo sapiens gateway clone IMAGE:100016733 5' read PPP1R1B mRNA.
KJ894942 - Synthetic construct Homo sapiens clone ccsbBroadEn_04336 PPP1R1B gene, encodes complete protein.
BT007076 - Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32) mRNA, complete cds.
BX648641 - Homo sapiens mRNA; cDNA DKFZp686C19219 (from clone DKFZp686C19219).
AK123950 - Homo sapiens cDNA FLJ41956 fis, clone PROST2019781.
AK124417 - Homo sapiens cDNA FLJ42426 fis, clone BLADE2004389.
AF233349 - Homo sapiens neuronal phosphoprotein DARPP-32 mRNA, partial cds.
JD532461 - Sequence 513485 from Patent EP1572962.
JD507973 - Sequence 488997 from Patent EP1572962.
JD156574 - Sequence 137598 from Patent EP1572962.
JD214980 - Sequence 196004 from Patent EP1572962.
JD417980 - Sequence 399004 from Patent EP1572962.
JD193719 - Sequence 174743 from Patent EP1572962.
JD149953 - Sequence 130977 from Patent EP1572962.
JD251698 - Sequence 232722 from Patent EP1572962.
JD387713 - Sequence 368737 from Patent EP1572962.
JD462907 - Sequence 443931 from Patent EP1572962.
JD548994 - Sequence 530018 from Patent EP1572962.
JD208055 - Sequence 189079 from Patent EP1572962.
JD357808 - Sequence 338832 from Patent EP1572962.
JD019931 - Sequence 955 from Patent EP1572962.
JD033308 - Sequence 14332 from Patent EP1572962.
JD371886 - Sequence 352910 from Patent EP1572962.
JD555057 - Sequence 536081 from Patent EP1572962.
JD420589 - Sequence 401613 from Patent EP1572962.
MA448025 - JP 2018138019-A/19951: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_fosbPathway - FOSB gene expression and drug abuse
h_ck1Pathway - Regulation of ck1/cdk5 by type 1 glutamate receptors

Reactome (by CSHL, EBI, and GO)

Protein Q9UD71 (Reactome details) participates in the following event(s):

R-HSA-180047 CDK5 phosphorylates DARPP-32 on Thr75
R-HSA-201790 DARPP-32 is dephosphorylated on Thr75 by PP2A
R-HSA-180038 DARPP-32 phosphorylated on T34 binds to PP1, inhibiting its function
R-HSA-201787 PPP3 complex dephosphorylates DARPP-32 on Thr34
R-HSA-177275 PKA phosphorylates DARPP-32 on Thr34
R-HSA-180073 DARPP-32 phosphorylated on Thr75 binds to PKA, inhibiting its function
R-HSA-180024 DARPP-32 events
R-HSA-111885 Opioid Signalling
R-HSA-418594 G alpha (i) signalling events
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: DARPP32, ENST00000254079.1, ENST00000254079.2, ENST00000254079.3, ENST00000254079.4, ENST00000254079.5, ENST00000254079.6, ENST00000254079.7, ENST00000254079.8, NM_032192, PPR1B_HUMAN, Q547V9, Q547W0, Q9H7G1, Q9UD71, uc317fnd.1, uc317fnd.2
UCSC ID: ENST00000254079.9_4
RefSeq Accession: NM_032192.4
Protein: Q9UD71 (aka PPR1B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.