ID:PO5F1_HUMAN DESCRIPTION: RecName: Full=POU domain, class 5, transcription factor 1; AltName: Full=Octamer-binding protein 3; Short=Oct-3; AltName: Full=Octamer-binding protein 4; Short=Oct-4; AltName: Full=Octamer-binding transcription factor 3; Short=OTF-3; FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. SUBUNIT: Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PKM. Interacts with WWP2. INTERACTION: Q9UJU5:FOXD3; NbExp=2; IntAct=EBI-475687, EBI-475674; SUBCELLULAR LOCATION: Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern. TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. DEVELOPMENTAL STAGE: Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. INDUCTION: Transcriptional activity is positively regulated by PKM. DOMAIN: The POU-specific domain mediates interaction with PKM. PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). PTM: Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity). BIOTECHNOLOGY: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis. SIMILARITY: Belongs to the POU transcription factor family. Class- 5 subfamily. SIMILARITY: Contains 1 homeobox DNA-binding domain. SIMILARITY: Contains 1 POU-specific domain. WEB RESOURCE: Name=Wikipedia; Note=Oct-4 entry; URL="http://en.wikipedia.org/wiki/Oct-4";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q01860
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000976 transcription regulatory region sequence-specific DNA binding GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0001227 transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding GO:0003677 DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0003723 RNA binding GO:0005515 protein binding GO:0008134 transcription factor binding GO:0031625 ubiquitin protein ligase binding GO:0035198 miRNA binding GO:0043565 sequence-specific DNA binding GO:0044212 transcription regulatory region DNA binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0001714 endodermal cell fate specification GO:0001824 blastocyst development GO:0003130 BMP signaling pathway involved in heart induction GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0007275 multicellular organism development GO:0009611 response to wounding GO:0009653 anatomical structure morphogenesis GO:0009786 regulation of asymmetric cell division GO:0010468 regulation of gene expression GO:0035019 somatic stem cell population maintenance GO:0042789 mRNA transcription from RNA polymerase II promoter GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0060391 positive regulation of SMAD protein import into nucleus GO:0060795 cell fate commitment involved in formation of primary germ layer GO:0060913 cardiac cell fate determination GO:0060965 negative regulation of gene silencing by miRNA GO:0090081 regulation of heart induction by regulation of canonical Wnt signaling pathway GO:0090263 positive regulation of canonical Wnt signaling pathway GO:0090308 regulation of methylation-dependent chromatin silencing
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
