ID:OFUT2_HUMAN DESCRIPTION: RecName: Full=GDP-fucose protein O-fucosyltransferase 2; EC=2.4.1.221; AltName: Full=Peptide-O-fucosyltransferase 2; Short=O-FucT-2; Flags: Precursor; FUNCTION: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. CATALYTIC ACTIVITY: Transfers an alpha-L-fucosyl residue from GDP- beta-L-fucose to the serine hydroxy group of a protein acceptor. ENZYME REGULATION: Inhibited by EDTA and by Zn(2+). BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=9.8 uM for GDP-fucose; PATHWAY: Protein modification; protein glycosylation. SUBCELLULAR LOCATION: Endoplasmic reticulum (By similarity). TISSUE SPECIFICITY: Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart. SIMILARITY: Belongs to the glycosyltransferase 68 family. SEQUENCE CAUTION: Sequence=BAA76802.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAB90496.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/"; WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=Peptide-O-fucosyltransferase 2; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_610";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Y2G5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001707 mesoderm formation GO:0005975 carbohydrate metabolic process GO:0006004 fucose metabolic process GO:0006486 protein glycosylation GO:0010468 regulation of gene expression GO:0010717 regulation of epithelial to mesenchymal transition GO:0036065 fucosylation GO:0036066 protein O-linked fucosylation GO:0051046 regulation of secretion
BC000626 - Homo sapiens protein O-fucosyltransferase 2, mRNA (cDNA clone IMAGE:3347416), partial cds. LF384653 - JP 2014500723-A/192156: Polycomb-Associated Non-Coding RNAs. BC064623 - Homo sapiens protein O-fucosyltransferase 2, mRNA (cDNA clone MGC:74914 IMAGE:5727996), complete cds. AL110285 - Homo sapiens mRNA; cDNA DKFZp434E171 (from clone DKFZp434E171). AB023175 - Homo sapiens KIAA0958 mRNA for KIAA0958 protein. AJ302079 - Homo sapiens partial mRNA for C21orf80 protein, form B. AJ302080 - Homo sapiens mRNA for C21orf80 protein, form A. AY066015 - Homo sapiens C21orf80 protein form C (C21orf80) mRNA, complete cds. BC011044 - Homo sapiens protein O-fucosyltransferase 2, mRNA (cDNA clone IMAGE:4156484), complete cds. DQ590428 - Homo sapiens piRNA piR-57540, complete sequence. AJ575591 - Homo sapiens mRNA for putative protein-O-fucosyltransferase (FUT13 gene). AK290732 - Homo sapiens cDNA FLJ76271 complete cds, highly similar to Homo sapiens protein O-fucosyltransferase 2 (POFUT2), transcript variant 3, mRNA. LF322978 - JP 2014500723-A/130481: Polycomb-Associated Non-Coding RNAs. LF322977 - JP 2014500723-A/130480: Polycomb-Associated Non-Coding RNAs. AB384059 - Synthetic construct DNA, clone: pF1KSDA0958, Homo sapiens POFUT2 gene for GDP-fucose protein O-fucosyltransferase 2 precursor, complete cds, without stop codon, in Flexi system. LF322976 - JP 2014500723-A/130479: Polycomb-Associated Non-Coding RNAs. LF322975 - JP 2014500723-A/130478: Polycomb-Associated Non-Coding RNAs. AK298080 - Homo sapiens cDNA FLJ56685 complete cds, highly similar to GDP-fucose protein O-fucosyltransferase 2 precursor (EC 2.4.1.221). HG380510 - Homo sapiens mRNA for protein O-fucosyltransferase 2 (POFUT2 gene), variant 1. LF322974 - JP 2014500723-A/130477: Polycomb-Associated Non-Coding RNAs. AK294966 - Homo sapiens cDNA FLJ56644 complete cds, highly similar to GDP-fucose protein O-fucosyltransferase 2 precursor (EC 2.4.1.221). HG380511 - Homo sapiens mRNA for protein O-fucosyltransferase 2 (POFUT2 gene), variant 2. JD476116 - Sequence 457140 from Patent EP1572962. MA620230 - JP 2018138019-A/192156: Polycomb-Associated Non-Coding RNAs. MA558555 - JP 2018138019-A/130481: Polycomb-Associated Non-Coding RNAs. MA558554 - JP 2018138019-A/130480: Polycomb-Associated Non-Coding RNAs. MA558553 - JP 2018138019-A/130479: Polycomb-Associated Non-Coding RNAs. MA558552 - JP 2018138019-A/130478: Polycomb-Associated Non-Coding RNAs. MA558551 - JP 2018138019-A/130477: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9Y2G5 (Reactome details) participates in the following event(s):
R-HSA-5173192 POFUT2 transfers fucose to TSR domain-containing proteins R-HSA-5173214 O-glycosylation of TSR domain-containing proteins R-HSA-5173105 O-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
