ID:PMF1_HUMAN DESCRIPTION: RecName: Full=Polyamine-modulated factor 1; Short=PMF-1; FUNCTION: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT. SUBUNIT: Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Interacts with COPS7A. Interacts via its coiled- coil domain with the leucine-zipper domain of NFE2L2. The interaction with NFE2L2 is required for the transcriptional regulation of SSAT. SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore. TISSUE SPECIFICITY: Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver. INDUCTION: By polyamine analogs in analog-sensitive H157 cells. SEQUENCE CAUTION: Sequence=AAD50081.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAH10730.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAH10730.1; Type=Frameshift; Positions=75;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q6P1K2
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK124646 - Homo sapiens cDNA FLJ42655 fis, clone BRACE3045033, moderately similar to Homo sapiens polyamine-modulated factor 1 (PMF1). AK307287 - Homo sapiens cDNA, FLJ97235. AK310694 - Homo sapiens cDNA, FLJ17736. AK290260 - Homo sapiens cDNA FLJ78103 complete cds, highly similar to Homo sapiens polyamine-modulated factor 1, mRNA. AK022523 - Homo sapiens cDNA FLJ12461 fis, clone NT2RM1000746, highly similar to Homo sapiens polyamine modulated factor-1 (PMF1) mRNA. AK289490 - Homo sapiens cDNA FLJ78329 complete cds, highly similar to Homo sapiens polyamine-modulated factor 1, mRNA. BC065031 - Homo sapiens polyamine-modulated factor 1, mRNA (cDNA clone MGC:75061 IMAGE:6148250), complete cds. BC033656 - Homo sapiens polyamine-modulated factor 1, mRNA (cDNA clone IMAGE:3909927). CU688008 - Synthetic construct Homo sapiens gateway clone IMAGE:100021605 5' read PMF1 mRNA. EU832588 - Synthetic construct Homo sapiens clone HAIB:100067617; DKFZo004G0330 polyamine-modulated factor 1 protein (PMF1) gene, encodes complete protein. KJ898390 - Synthetic construct Homo sapiens clone ccsbBroadEn_07784 PMF1 gene, encodes complete protein. EU832507 - Synthetic construct Homo sapiens clone HAIB:100067536; DKFZo008G0329 polyamine-modulated factor 1 protein (PMF1) gene, encodes complete protein. AB464036 - Synthetic construct DNA, clone: pF1KB7581, Homo sapiens PMF1 gene for polyamine-modulated factor 1, without stop codon, in Flexi system. DQ890582 - Synthetic construct clone IMAGE:100003212; FLH164128.01X; RZPDo839C12162D polyamine-modulated factor 1 (PMF1) gene, encodes complete protein. DQ893741 - Synthetic construct Homo sapiens clone IMAGE:100008201; FLH164124.01L; RZPDo839C12161D polyamine-modulated factor 1 (PMF1) gene, encodes complete protein. KJ898389 - Synthetic construct Homo sapiens clone ccsbBroadEn_07783 PMF1 gene, encodes complete protein. AL080101 - Homo sapiens mRNA; cDNA DKFZp564L0472 (from clone DKFZp564L0472). BC056417 - Homo sapiens polyamine-modulated factor 1, mRNA (cDNA clone IMAGE:5760174), partial cds. AF141310 - Homo sapiens polyamine modulated factor-1 (PMF1) mRNA, complete cds. BC050735 - Homo sapiens polyamine-modulated factor 1, mRNA (cDNA clone IMAGE:6067207), partial cds. JD171734 - Sequence 152758 from Patent EP1572962. JD086690 - Sequence 67714 from Patent EP1572962. JD104424 - Sequence 85448 from Patent EP1572962. JD136874 - Sequence 117898 from Patent EP1572962. JD278306 - Sequence 259330 from Patent EP1572962. JD457707 - Sequence 438731 from Patent EP1572962. JD067271 - Sequence 48295 from Patent EP1572962. JD450205 - Sequence 431229 from Patent EP1572962. JD054722 - Sequence 35746 from Patent EP1572962. JD400211 - Sequence 381235 from Patent EP1572962. JD352687 - Sequence 333711 from Patent EP1572962. JD384321 - Sequence 365345 from Patent EP1572962. JD115236 - Sequence 96260 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q6P1K2 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
