Human Gene PHB1 (ENST00000300408.8_7) from GENCODE V47lift37
  Description: prohibitin 1, transcript variant 2 (from RefSeq NM_002634.4)
Gencode Transcript: ENST00000300408.8_7
Gencode Gene: ENSG00000167085.14_18
Transcript (Including UTRs)
   Position: hg19 chr17:47,481,414-47,492,244 Size: 10,831 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr17:47,482,354-47,490,626 Size: 8,273 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:47,481,414-47,492,244)mRNA (may differ from genome)Protein (272 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PHB_HUMAN
DESCRIPTION: RecName: Full=Prohibitin;
FUNCTION: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
SUBUNIT: Interacts with PHB2.
INTERACTION: Q496Y0:LONRF3; NbExp=2; IntAct=EBI-354213, EBI-2690768; O14980:XPO1; NbExp=2; IntAct=EBI-354213, EBI-355867;
SUBCELLULAR LOCATION: Mitochondrion inner membrane (By similarity).
TISSUE SPECIFICITY: Widely expressed in different tissues.
DEVELOPMENTAL STAGE: Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
INDUCTION: Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.
SIMILARITY: Belongs to the prohibitin family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 78.78 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 2119.55 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.5075-0.140 Picture PostScript Text
3' UTR -349.70940-0.372 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001107 - Band_7
IPR000163 - Prohibitin

Pfam Domains:
PF01145 - SPFH domain / Band 7 family

SCOP Domains:
117892 - Band 7/SPFH domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1LU7 - Model


ModBase Predicted Comparative 3D Structure on P35232
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001850 complement component C3a binding
GO:0001851 complement component C3b binding
GO:0005515 protein binding
GO:0008022 protein C-terminus binding
GO:0019899 enzyme binding
GO:0031871 proteinase activated receptor binding
GO:0042826 histone deacetylase binding
GO:0044212 transcription regulatory region DNA binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001649 osteoblast differentiation
GO:0006355 regulation of transcription, DNA-templated
GO:0006851 mitochondrial calcium ion transport
GO:0007005 mitochondrion organization
GO:0007165 signal transduction
GO:0008285 negative regulation of cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010942 positive regulation of cell death
GO:0010944 negative regulation of transcription by competitive promoter binding
GO:0016575 histone deacetylation
GO:0030308 negative regulation of cell growth
GO:0042177 negative regulation of protein catabolic process
GO:0042981 regulation of apoptotic process
GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
GO:0045892 negative regulation of transcription, DNA-templated
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045917 positive regulation of complement activation
GO:0050821 protein stabilization
GO:0050847 progesterone receptor signaling pathway
GO:0060766 negative regulation of androgen receptor signaling pathway
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071354 cellular response to interleukin-6
GO:0071897 DNA biosynthetic process
GO:2000323 negative regulation of glucocorticoid receptor signaling pathway

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005769 early endosome
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0043209 myelin sheath
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  KJ891792 - Synthetic construct Homo sapiens clone ccsbBroadEn_01186 PHB gene, encodes complete protein.
AK223181 - Homo sapiens mRNA for prohibitin variant, clone: LNG13941.
JD124458 - Sequence 105482 from Patent EP1572962.
JD295555 - Sequence 276579 from Patent EP1572962.
JD040262 - Sequence 21286 from Patent EP1572962.
JD434303 - Sequence 415327 from Patent EP1572962.
JD061295 - Sequence 42319 from Patent EP1572962.
JD236588 - Sequence 217612 from Patent EP1572962.
JD562792 - Sequence 543816 from Patent EP1572962.
JD311338 - Sequence 292362 from Patent EP1572962.
JD385550 - Sequence 366574 from Patent EP1572962.
JD230532 - Sequence 211556 from Patent EP1572962.
JD079043 - Sequence 60067 from Patent EP1572962.
JD163531 - Sequence 144555 from Patent EP1572962.
JD196178 - Sequence 177202 from Patent EP1572962.
JD107273 - Sequence 88297 from Patent EP1572962.
JD154887 - Sequence 135911 from Patent EP1572962.
JD112822 - Sequence 93846 from Patent EP1572962.
JD174689 - Sequence 155713 from Patent EP1572962.
AK302258 - Homo sapiens cDNA FLJ51562 complete cds, highly similar to Prohibitin.
JD452253 - Sequence 433277 from Patent EP1572962.
JD217127 - Sequence 198151 from Patent EP1572962.
JD305948 - Sequence 286972 from Patent EP1572962.
AK307767 - Homo sapiens cDNA, FLJ97715.
AK290766 - Homo sapiens cDNA FLJ78511 complete cds, highly similar to Homo sapiens prohibitin (PHB), mRNA.
JD326202 - Sequence 307226 from Patent EP1572962.
BC095460 - Homo sapiens prohibitin, mRNA (cDNA clone MGC:111350 IMAGE:30557359), complete cds.
S85655 - prohibitin [human, mRNA, 1043 nt].
BC013401 - Homo sapiens prohibitin, mRNA (cDNA clone MGC:3926 IMAGE:3010198), complete cds.
GQ900914 - Homo sapiens clone HEL-T-26 epididymis secretory sperm binding protein mRNA, complete cds.
E05692 - Human cDNA encoding prohibitin.
JD071398 - Sequence 52422 from Patent EP1572962.
JD050527 - Sequence 31551 from Patent EP1572962.
JD104535 - Sequence 85559 from Patent EP1572962.
JD209856 - Sequence 190880 from Patent EP1572962.
AY577780 - Homo sapiens prohibitin mRNA, complete cds.
FJ224341 - Homo sapiens epididymis luminal protein 215 (HEL-215) mRNA, complete cds.
FJ460516 - Homo sapiens epididymis secretory sperm binding protein Li 54e (HEL-S-54e) mRNA, complete cds.
AK312649 - Homo sapiens cDNA, FLJ93035, Homo sapiens prohibitin (PHB), mRNA.
BT007411 - Homo sapiens prohibitin mRNA, complete cds.
CR541706 - Homo sapiens full open reading frame cDNA clone RZPDo834G0928D for gene PHB, prohibitin; complete cds, without stopcodon.
AB464069 - Synthetic construct DNA, clone: pF1KB8223, Homo sapiens PHB gene for prohibitin, without stop codon, in Flexi system.
AM393620 - Synthetic construct Homo sapiens clone IMAGE:100002560 for hypothetical protein (PHB gene).
JD369743 - Sequence 350767 from Patent EP1572962.
JD059045 - Sequence 40069 from Patent EP1572962.
JD145831 - Sequence 126855 from Patent EP1572962.
JD248978 - Sequence 230002 from Patent EP1572962.
JD362891 - Sequence 343915 from Patent EP1572962.
JD446098 - Sequence 427122 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P35232 (Reactome details) participates in the following event(s):

R-HSA-8949661 C2orf47:AFG3L2 binds the transit peptide of SMDT1
R-HSA-8949649 PMPCA:PMPCB cleaves the transit peptide of proSMDT1 (proEMRE)
R-HSA-8949664 Processing of SMDT1
R-HSA-8949215 Mitochondrial calcium ion transport
R-HSA-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: B4DY47, ENST00000300408.1, ENST00000300408.2, ENST00000300408.3, ENST00000300408.4, ENST00000300408.5, ENST00000300408.6, ENST00000300408.7, NM_002634, P35232, PHB, PHB1 , PHB1_HUMAN, Q4VBQ0, uc317mph.1, uc317mph.2
UCSC ID: ENST00000300408.8_7
RefSeq Accession: NM_002634.4
Protein: P35232 (aka PHB_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.