ID:PARD3_HUMAN DESCRIPTION: RecName: Full=Partitioning defective 3 homolog; Short=PAR-3; Short=PARD-3; AltName: Full=Atypical PKC isotype-specific-interacting protein; Short=ASIP; AltName: Full=CTCL tumor antigen se2-5; AltName: Full=PAR3-alpha; FUNCTION: Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. SUBUNIT: Interacts (via PDZ 1 domain) with F11R/JAM1, PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI and CDH5. Interacts (via PDZ 3 domain) with PTEN (via C-terminus) (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with LIMK2, AURKA and AURKB. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with ECT2 and FBF1. INTERACTION: Q9NPB6:PARD6A; NbExp=7; IntAct=EBI-81968, EBI-81876; Q9BYG5:PARD6B; NbExp=4; IntAct=EBI-81968, EBI-295391; Q9JK83:Pard6b (xeno); NbExp=2; IntAct=EBI-81968, EBI-81861; Q9BYG4:PARD6G; NbExp=3; IntAct=EBI-81968, EBI-295417; Q8ND90:PNMA1; NbExp=4; IntAct=EBI-81968, EBI-302345; P41743:PRKCI; NbExp=3; IntAct=EBI-81968, EBI-286199; Q04917:YWHAH; NbExp=6; IntAct=EBI-81968, EBI-306940; P63104:YWHAZ; NbExp=3; IntAct=EBI-81968, EBI-347088; SUBCELLULAR LOCATION: Endomembrane system. Cell junction. Cell junction, tight junction. Cell membrane. Cytoplasm, cell cortex (By similarity). Cytoplasm, cytoskeleton. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. TISSUE SPECIFICITY: Widely expressed. DOMAIN: Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization (By similarity). DOMAIN: The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids (By similarity). PTM: Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2. PTM: Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity. MISCELLANEOUS: Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas. SIMILARITY: Belongs to the PAR3 family. SIMILARITY: Contains 3 PDZ (DHR) domains. SEQUENCE CAUTION: Sequence=AAG33676.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=BAA91366.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAB55330.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8TEW0
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK000761 - Homo sapiens cDNA FLJ20754 fis, clone HEP02246. AX765704 - Sequence 20 from Patent WO02055706. AF196185 - Homo sapiens atypical PKC isotype-specific interacting protein long variant mRNA, complete cds. BC071566 - Homo sapiens par-3 partitioning defective 3 homolog (C. elegans), mRNA (cDNA clone MGC:87063 IMAGE:30344732), complete cds. BC011711 - Homo sapiens par-3 partitioning defective 3 homolog (C. elegans), mRNA (cDNA clone IMAGE:3939370), partial cds. AK024668 - Homo sapiens cDNA: FLJ21015 fis, clone CAE05730, highly similar to AF252293 Homo sapiens PAR3 (PAR3) mRNA. AB073671 - Homo sapiens mRNA for PAR3, complete cds. AF252293 - Homo sapiens partitioning-defective 3 splice variant c (PAR3) mRNA, complete cds, alternatively spliced. AF332593 - Homo sapiens atypical PKC isotype-specific interacting protein long variant b mRNA, complete cds, alternatively spliced. AF467002 - Homo sapiens partitioning-defective 3 protein splice variant a (PARD3) mRNA, complete cds, alternatively spliced. AF467003 - Homo sapiens partitioning-defective 3 protein splice variant b (PARD3) mRNA, complete cds, alternatively spliced. AF467004 - Homo sapiens partitioning-defective 3 protein splice variant d (PARD3) mRNA, complete cds, alternatively spliced. AF467005 - Homo sapiens partitioning-defective 3 protein splice variant e (PARD3) mRNA, complete cds, alternatively spliced. AF467006 - Homo sapiens partitioning-defective 3 protein splice variant f (PARD3) mRNA, complete cds, alternatively spliced. AX765715 - Sequence 31 from Patent WO02055706. AX765745 - Sequence 61 from Patent WO02055706. AX765714 - Sequence 30 from Patent WO02055706. AF454059 - Homo sapiens SE2-5T2 protein mRNA, complete cds. AK027735 - Homo sapiens cDNA FLJ14829 fis, clone OVARC1000945, moderately similar to Rattus norvegicus mRNA for atypical PKC specific binding protein. AF196186 - Homo sapiens atypical PKC isotype-specific interacting protein short variant mRNA, complete cds. AF332592 - Homo sapiens atypical PKC isotype-specific interacting protein short variant b mRNA, complete cds, alternatively spliced. AX765716 - Sequence 32 from Patent WO02055706. AF454057 - Homo sapiens SE2-5LT1 protein mRNA, partial cds. AF454058 - Homo sapiens SE2-5L16 protein mRNA, partial cds. AX765707 - Sequence 23 from Patent WO02055706. AX765706 - Sequence 22 from Patent WO02055706. AK172827 - Homo sapiens cDNA FLJ23988 fis, clone HRC01549. AX765723 - Sequence 39 from Patent WO02055706. AX765722 - Sequence 38 from Patent WO02055706. AX765705 - Sequence 21 from Patent WO02055706. AX765718 - Sequence 34 from Patent WO02055706. AK025892 - Homo sapiens cDNA: FLJ22239 fis, clone HRC02208, highly similar to AF252293 Homo sapiens PAR3 (PAR3) mRNA. AF177228 - Homo sapiens CTCL tumor antigen se2-5 mRNA, partial cds. AX765713 - Sequence 29 from Patent WO02055706. AX765712 - Sequence 28 from Patent WO02055706. AX765711 - Sequence 27 from Patent WO02055706. AX765709 - Sequence 25 from Patent WO02055706. AX765708 - Sequence 24 from Patent WO02055706. JD073121 - Sequence 54145 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q8TEW0 (Reactome details) participates in the following event(s):
R-HSA-419981 Recruitment of PAR-3:PAR-6:aPKC complex to tight junctions R-HSA-2134506 TGFBR1 is recruited to tight junctions by PARD6A R-NUL-2161147 TGFBR1 is recruited to tight junction by binding Pard6a R-HSA-2134519 TGFBR2 is recruited to tight junctions after TGF-beta stimulation R-HSA-2134532 TGFBR2 phosphorylates TGFBR1 and PARD6A R-HSA-2160932 SMURF1 binds phosphorylated PARD6A R-NUL-2161165 TGFBR2 phosphorylates Pard6a R-NUL-2161160 TGFBR2 is recruited to tight junctions-associated, Pard6a-bound, TGFBR1 after TGF-beta stimulation R-HSA-2160935 SMURF1 ubiquitinates RHOA R-HSA-420029 Tight junction interactions R-HSA-421270 Cell-cell junction organization R-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-HSA-446728 Cell junction organization R-HSA-170834 Signaling by TGF-beta Receptor Complex R-HSA-1500931 Cell-Cell communication R-HSA-9006936 Signaling by TGF-beta family members R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
