Human Gene P2RY4 (ENST00000374519.4_5) from GENCODE V47lift37
  Description: pyrimidinergic receptor P2Y4 (from RefSeq NM_002565.4)
Gencode Transcript: ENST00000374519.4_5
Gencode Gene: ENSG00000186912.7_7
Transcript (Including UTRs)
   Position: hg19 chrX:69,478,016-69,480,054 Size: 2,039 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chrX:69,478,377-69,479,474 Size: 1,098 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:69,478,016-69,480,054)mRNA (may differ from genome)Protein (365 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: P2RY4_HUMAN
DESCRIPTION: RecName: Full=P2Y purinoceptor 4; Short=P2Y4; AltName: Full=P2P; AltName: Full=Uridine nucleotide receptor; Short=UNR;
FUNCTION: Receptor for UTP and UDP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Not activated by ATP or ADP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Pancreas.
PTM: Phosphorylation of Ser-333 and Ser-334 is a key step in agonist-dependent desensitization and loss of surface P2RY4. This phosphorylation does not involve PKC, nor other calcium activated kinases.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.08 RPKM in Small Intestine - Terminal Ileum
Total median expression: 5.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -233.10580-0.402 Picture PostScript Text
3' UTR -109.70361-0.304 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR002286 - P2_purnocptor
IPR000018 - P2Y4_purnocptor

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2B6Q - Model


ModBase Predicted Comparative 3D Structure on P51582
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005524 ATP binding
GO:0015065 uridine nucleotide receptor activity
GO:0045028 G-protein coupled purinergic nucleotide receptor activity
GO:0045030 UTP-activated nucleotide receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0030321 transepithelial chloride transport
GO:0035589 G-protein coupled purinergic nucleotide receptor signaling pathway
GO:0071380 cellular response to prostaglandin E stimulus

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane


-  Descriptions from all associated GenBank mRNAs
  BC095503 - Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4, mRNA (cDNA clone MGC:111710 IMAGE:30706597), complete cds.
JD246306 - Sequence 227330 from Patent EP1572962.
JD297460 - Sequence 278484 from Patent EP1572962.
JD222448 - Sequence 203472 from Patent EP1572962.
JD323162 - Sequence 304186 from Patent EP1572962.
JD449204 - Sequence 430228 from Patent EP1572962.
JD484250 - Sequence 465274 from Patent EP1572962.
BC096067 - Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4, mRNA (cDNA clone MGC:116702 IMAGE:40000385), complete cds.
BC096068 - Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4, mRNA (cDNA clone MGC:116703 IMAGE:40000387), complete cds.
BC096069 - Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4, mRNA (cDNA clone MGC:116704 IMAGE:40000391), complete cds.
BC096070 - Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4, mRNA (cDNA clone MGC:116705 IMAGE:40000394), complete cds.
JD170946 - Sequence 151970 from Patent EP1572962.
JD214714 - Sequence 195738 from Patent EP1572962.
KJ891742 - Synthetic construct Homo sapiens clone ccsbBroadEn_01136 P2RY4 gene, encodes complete protein.
FJ599702 - Homo sapiens cell-line Caco-2 pyrimidinergic receptor P2Y4 (P2RY4) mRNA, partial cds.
FJ599701 - Homo sapiens cell-line MCF-7 pyrimidinergic receptor P2Y4 (P2RY4) mRNA, partial cds.
JD304954 - Sequence 285978 from Patent EP1572962.
JD498450 - Sequence 479474 from Patent EP1572962.
JD346796 - Sequence 327820 from Patent EP1572962.
JD197265 - Sequence 178289 from Patent EP1572962.
JD219337 - Sequence 200361 from Patent EP1572962.
JD343199 - Sequence 324223 from Patent EP1572962.
JD045380 - Sequence 26404 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P51582 (Reactome details) participates in the following event(s):

R-HSA-417898 P2Y4 receptor can bind to UTP
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-417957 P2Y receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-418038 Nucleotide-like (purinergic) receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000374519.1, ENST00000374519.2, ENST00000374519.3, NM_002565, NRU, P2RY4_HUMAN, P51582, Q4VBB7, Q4VBB8, Q502W2, Q5JT22, uc318lqq.1, uc318lqq.2
UCSC ID: ENST00000374519.4_5
RefSeq Accession: NM_002565.4
Protein: P51582 (aka P2RY4_HUMAN or P2Y4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.