ID:NXF1_HUMAN DESCRIPTION: RecName: Full=Nuclear RNA export factor 1; AltName: Full=Tip-associated protein; AltName: Full=Tip-associating protein; AltName: Full=mRNA export factor TAP; FUNCTION: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. SUBUNIT: Heterodimer with NXT1. Interacts (via NTF2 domain) with NXT1. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Interacts with NXT2, E1B-AP5, RAE1, ALYREF/THOC4 and with several nucleoporins. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF1, UPF2, UPF3A, UPF3B, ALYREF/THOC4 and/or REFBP2. Found in a mRNA complex with RENT3A and RENT3B. Interacts with FYTTD1/UIF, EIF4A3, NUPL2 and THOC5. Interacts with Saimiriine herpesvirus 2 TIP protein. Interacts with human herpes virus 1 (HHV-1) ICP27 protein; this interaction allows efficient export of HHV-1 early and late transcripts. INTERACTION: P22575:- (xeno); NbExp=6; IntAct=EBI-398874, EBI-866709; O00571:DDX3X; NbExp=5; IntAct=EBI-398874, EBI-353779; Q08211:DHX9; NbExp=7; IntAct=EBI-398874, EBI-352022; Q9UKK6:NXT1; NbExp=2; IntAct=EBI-398874, EBI-301889; Q07955:SRSF1; NbExp=5; IntAct=EBI-398874, EBI-398920; SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Nucleus speckle. Cytoplasm. Note=Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. TISSUE SPECIFICITY: Expressed ubiquitously. DOMAIN: The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats. DOMAIN: The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs. DOMAIN: The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus. DOMAIN: The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594- NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins. DOMAIN: The leucine-rich repeats are essential for the export of mRNA from the nucleus. DOMAIN: The RNA-binding domain is a non-canonical RNP-type domain. SIMILARITY: Belongs to the NXF family. SIMILARITY: Contains 4 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 NTF2 domain. SIMILARITY: Contains 1 RRM (RNA recognition motif) domain. SIMILARITY: Contains 1 TAP-C domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UBU9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
CR749674 - Homo sapiens mRNA; cDNA DKFZp667O0311 (from clone DKFZp667O0311). BC028041 - Homo sapiens nuclear RNA export factor 1, mRNA (cDNA clone MGC:40096 IMAGE:5248449), complete cds. AB209915 - Homo sapiens mRNA for nuclear RNA export factor 1 variant protein. AK027192 - Homo sapiens cDNA: FLJ23539 fis, clone LNG08101, highly similar to AF126246 Homo sapiens tip-associated protein TAP mRNA. BC004904 - Homo sapiens nuclear RNA export factor 1, mRNA (cDNA clone MGC:4612 IMAGE:3504065), complete cds. BC017421 - Homo sapiens nuclear RNA export factor 1, mRNA (cDNA clone IMAGE:4687823), with apparent retained intron. JD164945 - Sequence 145969 from Patent EP1572962. JD447939 - Sequence 428963 from Patent EP1572962. JD548920 - Sequence 529944 from Patent EP1572962. U80073 - Human tip associating protein (TAP) mRNA, complete cds. AF112880 - Homo sapiens tip associating protein mRNA, complete cds. AF126246 - Homo sapiens tip-associated protein TAP mRNA, complete cds. AJ132712 - Homo sapiens mRNA for nuclear RNA export factor 1 (NXF1 gene). KJ893058 - Synthetic construct Homo sapiens clone ccsbBroadEn_02452 NXF1 gene, encodes complete protein. DQ892931 - Synthetic construct clone IMAGE:100005561; FLH190949.01X; RZPDo839F0976D nuclear RNA export factor 1 (NXF1) gene, encodes complete protein. DQ896180 - Synthetic construct Homo sapiens clone IMAGE:100010640; FLH190947.01L; RZPDo839F0966D nuclear RNA export factor 1 (NXF1) gene, encodes complete protein. AB528336 - Synthetic construct DNA, clone: pF1KB6112, Homo sapiens NXF1 gene for nuclear RNA export factor 1, without stop codon, in Flexi system. AK307961 - Homo sapiens cDNA, FLJ97909. AK307998 - Homo sapiens cDNA, FLJ97946. AK304082 - Homo sapiens cDNA FLJ61053 complete cds, highly similar to Nuclear RNA export factor 1. AK304743 - Homo sapiens cDNA FLJ57456 complete cds, highly similar to Nuclear RNA export factor 1. AK304137 - Homo sapiens cDNA FLJ59010 complete cds, highly similar to Nuclear RNA export factor 1. CU678798 - Synthetic construct Homo sapiens gateway clone IMAGE:100020341 5' read NXF1 mRNA.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9UBU9 (Reactome details) participates in the following event(s):
R-HSA-77587 Docking of Mature Replication Dependent Histone mRNA with the NPC R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-111439 Docking of Mature Histone mRNA complex:TAP at the NPC R-HSA-158447 Release of the Mature intronless derived mRNA, TAP, and Aly/Ref from the NPC R-HSA-158484 Release of the SLBP independent Histone mRNA from the NPC R-HSA-159050 Release of the Mature intronless transcript derived Histone mRNA:SLBP:eIF4E Complex R-HSA-158441 Transport of the Mature intronless transcript derived mRNA:TAP:Aly/Ref Complex through the NPC R-HSA-159046 Transport of the Mature IntronlessTranscript Derived Histone mRNA:SLBP:TAP:Aly/Ref complex through the NPC R-HSA-159101 NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B) R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-159230 Transport of the SLBP Dependant Mature mRNA R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159227 Transport of the SLBP independent Mature mRNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-8953854 Metabolism of RNA
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
