Human Gene NUP35 (ENST00000295119.9_7) from GENCODE V47lift37

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Description: nucleoporin 35, transcript variant 2 (from RefSeq NR_109856.2)
Gencode Transcript: ENST00000295119.9_7
Gencode Gene: ENSG00000163002.13_17
Transcript (Including UTRs)
Position: hg19 chr2:183,989,171-184,026,408 Size: 37,238 Total Exon Count: 9 Strand: +
Coding Region
Position: hg19 chr2:183,989,186-184,025,859 Size: 36,674 Coding Exon Count: 9

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	RNA Structure	Protein Structure	Other Species	GO Annotations	mRNA Descriptions
Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr2:183,989,171-184,026,408)			mRNA (may differ from genome)		Protein (326 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	Reactome	UniProtKB
Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: NUP53_HUMAN DESCRIPTION: RecName: Full=Nucleoporin NUP53; AltName: Full=35 kDa nucleoporin; AltName: Full=Mitotic phosphoprotein 44; Short=MP-44; AltName: Full=Nuclear pore complex protein Nup53; AltName: Full=Nucleoporin Nup35; FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. SUBUNIT: Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct. SUBCELLULAR LOCATION: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein. Note=Tightly associated with the nuclear membrane and lamina. SIMILARITY: Belongs to the Nup53 family. SIMILARITY: Contains 1 RRM Nup35-type domain.

Primer design for this transcript

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Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D000082 Acetaminophen D013749 Tetrachlorodibenzodioxin C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl C016403 2,4-dinitrotoluene C023514 2,6-dinitrotoluene D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide D002117 Calcitriol D002922 Ciguatoxins D002994 Clofibrate D003375 Coumestrol D004997 Ethinyl Estradiol D007649 Ketamine D010634 Phenobarbital D011794 Quercetin D012999 Soman D013629 Tamoxifen D013739 Testosterone C006780 bisphenol A C045950 propiconazole

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 13.37 RPKM in Testis Total median expression: 190.48 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
3' UTR	-128.50	549	-0.234	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR017389 - Nucleoporin_NUP53
IPR007846 - RRM_NUP35_dom

Pfam Domains:
PF05172 - Nup53/35/40-type RNA recognition motif
PF14605 - Nup53/35/40-type RNA recognition motif

SCOP Domains:
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q8NFH5


Front	Top	Side

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Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	Genome Browser	No ortholog
Gene Details	Gene Details		Gene Details	Gene Details
Gene Sorter	Gene Sorter		Gene Sorter	Gene Sorter
	RGD	Ensembl		WormBase
				Protein Sequence
				Alignment

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0003676 nucleic acid binding GO:0003697 single-stranded DNA binding GO:0005515 protein binding GO:0005543 phospholipid binding Biological Process: GO:0006355 regulation of transcription, DNA-templated GO:0006406 mRNA export from nucleus GO:0006607 NLS-bearing protein import into nucleus GO:0006913 nucleocytoplasmic transport GO:0006999 nuclear pore organization GO:0015031 protein transport GO:0016032 viral process GO:0051028 mRNA transport Cellular Component: GO:0005634 nucleus GO:0005635 nuclear envelope GO:0005643 nuclear pore GO:0005652 nuclear lamina GO:0005654 nucleoplasm GO:0005886 plasma membrane GO:0016020 membrane GO:0031965 nuclear membrane GO:0044613 nuclear pore central transport channel GO:0044615 nuclear pore nuclear basket

Descriptions from all associated GenBank mRNAs


	AK302350 - Homo sapiens cDNA FLJ57668 complete cds, highly similar to Nucleoporin NUP53. AF411516 - Homo sapiens mitotic phosphoprotein 44 mRNA, complete cds. AF514993 - Homo sapiens nucleoporin Nup35 mRNA, complete cds. BC061698 - Homo sapiens nucleoporin 35kDa, mRNA (cDNA clone MGC:62083 IMAGE:4132440), complete cds. BC022333 - Homo sapiens nucleoporin 35kDa, mRNA (cDNA clone IMAGE:4697029), containing frame-shift errors. AK289601 - Homo sapiens cDNA FLJ76599 complete cds, highly similar to Homo sapiens nucleoporin 35kDa (NUP35), transcript variant 2, mRNA. BC111016 - Homo sapiens nucleoporin 35kDa, mRNA (cDNA clone IMAGE:5176043), with apparent retained intron. BC035597 - Homo sapiens nucleoporin 35kDa, mRNA (cDNA clone IMAGE:5194278). AK290760 - Homo sapiens cDNA FLJ76613 complete cds, highly similar to Homo sapiens nucleoporin 35kDa (NUP35), transcript variant 1, mRNA. AK298199 - Homo sapiens cDNA FLJ60753 complete cds, highly similar to Nucleoporin NUP53. HQ448650 - Synthetic construct Homo sapiens clone IMAGE:100072089; CCSB011490_01 nucleoporin 35kDa (NUP35) gene, encodes complete protein. KJ895458 - Synthetic construct Homo sapiens clone ccsbBroadEn_04852 NUP35 gene, encodes complete protein. JD127617 - Sequence 108641 from Patent EP1572962. CU692362 - Synthetic construct Homo sapiens gateway clone IMAGE:100021373 5' read NUP35 mRNA. JD071523 - Sequence 52547 from Patent EP1572962. JD095225 - Sequence 76249 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein Q8NFH5 (Reactome details) participates in the following event(s): R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-165043 Rev multimer-bound HIV mRNA:Crm1:Ran:GTP complex associates with the NPC R-HSA-180622 Vpr binds nucleoporins R-HSA-180710 Rev:importin beta:B23 recruited to the nuclear pore R-HSA-2990882 CDK1 phosphorylates NUP98 R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC R-HSA-165047 Translocation of nuclear RNA transport complex to cytoplasm R-HSA-180732 Translocation of Rev:importin-beta:B23 to the nucleus R-HSA-2990880 NEK6/NEK7 phosphorylates NUP98 R-HSA-170796 NPC transports GCK1:GKRP from cytosol to nucleoplasm R-HSA-5578744 Importin-8 imports AGO2:miRNA into the nucleus R-HSA-6783483 tRNA:XPOT:RAN:GTP translocates from the nucleus to the cytosol R-HSA-3000348 RANBP2 SUMOylates SP100 with SUMO2 R-HSA-3000399 RANBP2 SUMOylates SP100 with SUMO1 R-HSA-3000411 RANBP2 SUMOylates PML with SUMO2 R-HSA-4570493 RANBP2 (NUP358) SUMOylates HNRNPC with SUMO1 R-HSA-4615872 RANBP2 SUMOylates HDAC4 with SUMO1 R-HSA-4615987 RANBP2 SUMOylates HDAC4 with SUMO2,3 R-HSA-5228508 RANBP2 SUMOylates PML with SUMO1 R-HSA-5228525 RANBP2 SUMOylates TOP2A with SUMO1 R-HSA-192627 Viral mRNA Export R-HSA-192925 Export of Spliced Viral mRNA R-HSA-5252041 NPC transports Hikeshi:HSP70s:ATP from cytosol to nucleoplasm R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054 Rev-mediated nuclear export of HIV RNA R-HSA-180910 Vpr-mediated nuclear import of PICs R-HSA-180746 Nuclear import of Rev protein R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-177243 Interactions of Rev with host cellular proteins R-HSA-176033 Interactions of Vpr with host cellular proteins R-HSA-2980766 Nuclear Envelope Breakdown R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-168276 NS1 Mediated Effects on Host Pathways R-HSA-1169408 ISG15 antiviral mechanism R-HSA-5578749 Transcriptional regulation by small RNAs R-HSA-6784531 tRNA processing in the nucleus R-HSA-3108214 SUMOylation of DNA damage response and repair proteins R-HSA-4570464 SUMOylation of RNA binding proteins R-HSA-4551638 SUMOylation of chromatin organization proteins R-HSA-4615885 SUMOylation of DNA replication proteins R-HSA-168325 Viral Messenger RNA Synthesis R-HSA-3371453 Regulation of HSF1-mediated heat shock response R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-162587 HIV Life Cycle R-HSA-162909 Host Interactions of HIV factors R-HSA-68875 Mitotic Prophase R-HSA-70171 Glycolysis R-HSA-168253 Host Interactions with Influenza Factors R-HSA-1169410 Antiviral mechanism by IFN-stimulated genes R-HSA-211000 Gene Silencing by RNA R-HSA-72306 tRNA processing R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins R-HSA-168273 Influenza Viral RNA Transcription and Replication R-HSA-3371556 Cellular response to heat stress R-HSA-8953854 Metabolism of RNA R-HSA-162906 HIV Infection R-HSA-68886 M Phase R-HSA-70326 Glucose metabolism R-HSA-168254 Influenza Infection R-HSA-913531 Interferon Signaling R-HSA-74160 Gene expression (Transcription) R-HSA-2990846 SUMOylation R-HSA-168255 Influenza Life Cycle R-HSA-2262752 Cellular responses to stress R-HSA-5663205 Infectious disease R-HSA-69278 Cell Cycle (Mitotic) R-HSA-71387 Metabolism of carbohydrates R-HSA-1280215 Cytokine Signaling in Immune system R-HSA-597592 Post-translational protein modification R-HSA-8953897 Cellular responses to external stimuli R-HSA-1643685 Disease R-HSA-1640170 Cell Cycle R-HSA-1430728 Metabolism R-HSA-168256 Immune System R-HSA-392499 Metabolism of proteins

Other Names for This Gene


	Alternate Gene Symbols: B4DP57, B4DYB4, ENST00000295119.1, ENST00000295119.2, ENST00000295119.3, ENST00000295119.4, ENST00000295119.5, ENST00000295119.6, ENST00000295119.7, ENST00000295119.8, MP44, NR_109856, NUP35_HUMAN, NUP53, Q4ZFZ9, Q53S95, Q8NFH5, Q8TDJ1, uc317ljy.1, uc317ljy.2 UCSC ID: ENST00000295119.9_7 RefSeq Accession: NM_138285.5 Protein: Q8NFH5 (aka NUP53_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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