Human Gene NPFF (ENST00000267017.4_7) from GENCODE V47lift37
  Description: neuropeptide FF-amide peptide precursor, transcript variant 1 (from RefSeq NM_003717.4)
Gencode Transcript: ENST00000267017.4_7
Gencode Gene: ENSG00000139574.9_9
Transcript (Including UTRs)
   Position: hg19 chr12:53,900,472-53,901,268 Size: 797 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr12:53,900,560-53,901,258 Size: 699 Coding Exon Count: 3 

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Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:53,900,472-53,901,268)mRNA (may differ from genome)Protein (113 aa)
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-  Comments and Description Text from UniProtKB
  ID: NPFF_HUMAN
DESCRIPTION: RecName: Full=FMRFamide-related peptides; Contains: RecName: Full=Neuropeptide SF; Short=NPSF; Contains: RecName: Full=Neuropeptide FF; Short=NPFF; Contains: RecName: Full=Neuropeptide AF; Short=NPAF; Flags: Precursor;
FUNCTION: Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NPFF
Diseases sorted by gene-association score: pain agnosia (2), agnosia (2), specific developmental disorder (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -14.8088-0.168 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008065 - FMRFamid-related_peptide

Pfam Domains:
PF15085 - Neuropeptide FF

ModBase Predicted Comparative 3D Structure on O15130
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001664 G-protein coupled receptor binding
GO:0005102 receptor binding
GO:0005184 neuropeptide hormone activity

Biological Process:
GO:0002438 acute inflammatory response to antigenic stimulus
GO:0003254 regulation of membrane depolarization
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0007218 neuropeptide signaling pathway
GO:0007268 chemical synaptic transmission
GO:0010459 negative regulation of heart rate
GO:0010469 regulation of receptor activity
GO:0021510 spinal cord development
GO:0030103 vasopressin secretion
GO:0032099 negative regulation of appetite
GO:0042493 response to drug
GO:0043278 response to morphine
GO:0045777 positive regulation of blood pressure
GO:0046676 negative regulation of insulin secretion
GO:0051930 regulation of sensory perception of pain
GO:0060079 excitatory postsynaptic potential
GO:0060135 maternal process involved in female pregnancy
GO:0070253 somatostatin secretion

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0030425 dendrite
GO:0031982 vesicle
GO:0043204 perikaryon
GO:0043679 axon terminus
GO:0098794 postsynapse


-  Descriptions from all associated GenBank mRNAs
  BC035758 - Homo sapiens neuropeptide FF-amide peptide precursor, mRNA (cDNA clone IMAGE:5764015), with apparent retained intron.
BC104234 - Homo sapiens neuropeptide FF-amide peptide precursor, mRNA (cDNA clone MGC:126084 IMAGE:40033026), complete cds.
BC104235 - Homo sapiens neuropeptide FF-amide peptide precursor, mRNA (cDNA clone MGC:126085 IMAGE:40033028), complete cds.
AF005271 - Homo sapiens FMRFamide-related prepropeptide mRNA, complete cds.
KJ901893 - Synthetic construct Homo sapiens clone ccsbBroadEn_11287 NPFF gene, encodes complete protein.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O15130 (Reactome details) participates in the following event(s):

R-HSA-389491 Two NPFF receptors can bind neuropeptide FF
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors
R-HSA-416476 G alpha (q) signalling events
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000267017.1, ENST00000267017.2, ENST00000267017.3, NM_003717, NPFF_HUMAN, O15130, Q3SXL4, uc317ijc.1, uc317ijc.2
UCSC ID: ENST00000267017.4_7
RefSeq Accession: NM_003717.4
Protein: O15130 (aka NPFF_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
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