Human Gene NOL8 (ENST00000442668.7_14) from GENCODE V47lift37
  Description: nucleolar protein 8, transcript variant 1 (from RefSeq NM_017948.6)
Gencode Transcript: ENST00000442668.7_14
Gencode Gene: ENSG00000198000.12_21
Transcript (Including UTRs)
   Position: hg19 chr9:95,059,640-95,087,632 Size: 27,993 Total Exon Count: 17 Strand: -
Coding Region
   Position: hg19 chr9:95,060,118-95,086,443 Size: 26,326 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:95,059,640-95,087,632)mRNA (may differ from genome)Protein (1167 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NOL8_HUMAN
DESCRIPTION: RecName: Full=Nucleolar protein 8; AltName: Full=Nucleolar protein Nop132;
FUNCTION: Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
SUBUNIT: Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA- dependent. Interacts with DDX47; the interaction is RNA-dependent.
SUBCELLULAR LOCATION: Nucleus, nucleolus. Note=Localizes in the nucleolar-organizing region during ribosome biogenesis.
TISSUE SPECIFICITY: Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
INDUCTION: Up-regulated in diffuse-type gastric cancers.
PTM: Phosphorylated (Probable).
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
SEQUENCE CAUTION: Sequence=BAA91356.1; Type=Frameshift; Positions=1136; Sequence=BAB14229.1; Type=Erroneous initiation; Sequence=BAB15003.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.95 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 310.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.9093-0.235 Picture PostScript Text
3' UTR -95.50478-0.200 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

SCOP Domains:
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q76FK4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0005515 protein binding

Biological Process:
GO:0006364 rRNA processing
GO:1902570 protein localization to nucleolus

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus


-  Descriptions from all associated GenBank mRNAs
  AB105104 - Homo sapiens NOL8 mRNA for nucleolar protein 8, complete cds.
AK024245 - Homo sapiens cDNA FLJ14183 fis, clone NT2RP2004920, weakly similar to TRANSCRIPTIONAL REGULATOR ATRX.
LF209787 - JP 2014500723-A/17290: Polycomb-Associated Non-Coding RNAs.
JD281221 - Sequence 262245 from Patent EP1572962.
JD067967 - Sequence 48991 from Patent EP1572962.
JD309583 - Sequence 290607 from Patent EP1572962.
JD245865 - Sequence 226889 from Patent EP1572962.
BC013788 - Homo sapiens nucleolar protein 8, mRNA (cDNA clone IMAGE:3853878), partial cds.
BC146810 - Homo sapiens nucleolar protein 8, mRNA (cDNA clone MGC:167036 IMAGE:8860369), complete cds.
AK024786 - Homo sapiens cDNA: FLJ21133 fis, clone CAS07019.
AK225322 - Homo sapiens mRNA for nucleolar protein 8 variant, clone: HEP08473.
AK000743 - Homo sapiens cDNA FLJ20736 fis, clone HEP08473.
BC035041 - Homo sapiens nucleolar protein 8, mRNA (cDNA clone IMAGE:4829474), with apparent retained intron.
CR627363 - Homo sapiens mRNA; cDNA DKFZp686P12242 (from clone DKFZp686P12242).
JD527348 - Sequence 508372 from Patent EP1572962.
JD172532 - Sequence 153556 from Patent EP1572962.
LF331627 - JP 2014500723-A/139130: Polycomb-Associated Non-Coding RNAs.
AB109030 - Homo sapiens Nop132 mRNA for Nop132, complete cds.
KJ902747 - Synthetic construct Homo sapiens clone ccsbBroadEn_12141 NOL8 gene, encodes complete protein.
LF331625 - JP 2014500723-A/139128: Polycomb-Associated Non-Coding RNAs.
BC064942 - Homo sapiens nucleolar protein 8, mRNA (cDNA clone IMAGE:6008261).
AK022755 - Homo sapiens cDNA FLJ12693 fis, clone NT2RP1000324.
AK307408 - Homo sapiens cDNA, FLJ97356.
AK297355 - Homo sapiens cDNA FLJ60085 partial cds, highly similar to Nucleolar protein 8.
BC008491 - Homo sapiens nucleolar protein 8, mRNA (cDNA clone IMAGE:4248937), with apparent retained intron.
LF331623 - JP 2014500723-A/139126: Polycomb-Associated Non-Coding RNAs.
LF331621 - JP 2014500723-A/139124: Polycomb-Associated Non-Coding RNAs.
LF331620 - JP 2014500723-A/139123: Polycomb-Associated Non-Coding RNAs.
AK001049 - Homo sapiens cDNA FLJ10187 fis, clone HEMBA1004596.
LF331617 - JP 2014500723-A/139120: Polycomb-Associated Non-Coding RNAs.
MA567204 - JP 2018138019-A/139130: Polycomb-Associated Non-Coding RNAs.
MA567202 - JP 2018138019-A/139128: Polycomb-Associated Non-Coding RNAs.
MA567200 - JP 2018138019-A/139126: Polycomb-Associated Non-Coding RNAs.
MA567198 - JP 2018138019-A/139124: Polycomb-Associated Non-Coding RNAs.
MA567197 - JP 2018138019-A/139123: Polycomb-Associated Non-Coding RNAs.
MA567194 - JP 2018138019-A/139120: Polycomb-Associated Non-Coding RNAs.
MA445364 - JP 2018138019-A/17290: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: C9orf34, ENST00000442668.1, ENST00000442668.2, ENST00000442668.3, ENST00000442668.4, ENST00000442668.5, ENST00000442668.6, NM_017948, NOL8 , NOL8_HUMAN, NOP132, Q5TCC7, Q5TCC8, Q5TCD3, Q5TCD5, Q5TCD6, Q5TCD7, Q76D35, Q76FK4, Q7L3E2, Q9H586, Q9H795, Q9H7W7, Q9H9J6, Q9NWA4, Q9NWM4, uc320kri.1, uc320kri.2
UCSC ID: ENST00000442668.7_14
RefSeq Accession: NM_017948.6
Protein: Q76FK4 (aka NOL8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.