ID:NINL_HUMAN DESCRIPTION: RecName: Full=Ninein-like protein; FUNCTION: Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis. SUBUNIT: Interacts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Isoform 2 interacts with LCA5 and USH2A. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm. TISSUE SPECIFICITY: Expressed in KYSE-150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. DOMAIN: The KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway. PTM: Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. PTM: Ubiquitinated by the APC/C complex leading to its degradation. SIMILARITY: Contains 4 EF-hand domains. SEQUENCE CAUTION: Sequence=BAA76824.1; Type=Erroneous initiation; Sequence=BAH11644.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAH11644.1; Type=Erroneous termination; Positions=405; Note=Translated as Gln;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Y2I6
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AB023197 - Homo sapiens KIAA0980 mRNA for KIAA0980 protein. EU718622 - Homo sapiens ninein-like protein isoform B mRNA, complete cds. AK309298 - Homo sapiens cDNA, FLJ99339. BC036380 - Homo sapiens ninein-like, mRNA (cDNA clone MGC:33226 IMAGE:5268454), complete cds. AK021854 - Homo sapiens cDNA FLJ11792 fis, clone HEMBA1006121. JD296574 - Sequence 277598 from Patent EP1572962. JD041199 - Sequence 22223 from Patent EP1572962. JD216061 - Sequence 197085 from Patent EP1572962. JD471051 - Sequence 452075 from Patent EP1572962. JD450136 - Sequence 431160 from Patent EP1572962. JD207881 - Sequence 188905 from Patent EP1572962. JD544790 - Sequence 525814 from Patent EP1572962. AB384073 - Synthetic construct DNA, clone: pF1KSDA0980, Homo sapiens NLP gene for ninein-like protein, complete cds, without stop codon, in Flexi system. BC113095 - Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein pseudogene 1, mRNA (cDNA clone IMAGE:40076101), **** WARNING: chimeric clone ****. KJ904409 - Synthetic construct Homo sapiens clone ccsbBroadEn_13803 hypothetical protein, encodes complete protein. AK093569 - Homo sapiens cDNA FLJ36250 fis, clone THYMU2002037. AX748206 - Sequence 1731 from Patent EP1308459. AK293991 - Homo sapiens cDNA FLJ56426 complete cds, weakly similar to Ninein.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9Y2I6 (Reactome details) participates in the following event(s):
R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-5617833 Cilium Assembly R-HSA-68877 Mitotic Prometaphase R-HSA-69275 G2/M Transition R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-68886 M Phase R-HSA-453274 Mitotic G2-G2/M phases R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle
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Common Gene Haplotype Alleles 
