ID:NEK9_HUMAN DESCRIPTION: RecName: Full=Serine/threonine-protein kinase Nek9; EC=2.7.11.1; AltName: Full=Nercc1 kinase; AltName: Full=Never in mitosis A-related kinase 9; Short=NimA-related protein kinase 9; AltName: Full=NimA-related kinase 8; Short=Nek8; FUNCTION: Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions. Sensitive to increasing concentration of detergents. It is not cell-cycle regulated but activity is higher in G0-arrested cells. SUBUNIT: Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. INTERACTION: O95166:GABARAP; NbExp=5; IntAct=EBI-1044009, EBI-712001; Q9H0R8:GABARAPL1; NbExp=6; IntAct=EBI-1044009, EBI-746969; P60520:GABARAPL2; NbExp=4; IntAct=EBI-1044009, EBI-720116; Q9GZQ8:MAP1LC3B; NbExp=2; IntAct=EBI-1044009, EBI-373144; Q9BXW4:MAP1LC3C; NbExp=2; IntAct=EBI-1044009, EBI-2603996; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. DEVELOPMENTAL STAGE: Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells. DOMAIN: Dimerizes through its coiled-coil domain. PTM: Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 6 RCC1 repeats. SEQUENCE CAUTION: Sequence=AAD31936.1; Type=Erroneous gene model prediction; Sequence=BAC02704.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8TD19
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
