Human Gene NADK (ENST00000341426.9_7) from GENCODE V47lift37
  Description: NAD kinase, transcript variant 1 (from RefSeq NM_023018.5)
Gencode Transcript: ENST00000341426.9_7
Gencode Gene: ENSG00000008130.15_9
Transcript (Including UTRs)
   Position: hg19 chr1:1,682,671-1,709,909 Size: 27,239 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr1:1,684,343-1,696,845 Size: 12,503 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:1,682,671-1,709,909)mRNA (may differ from genome)Protein (446 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NADK_HUMAN
DESCRIPTION: RecName: Full=NAD kinase; EC=2.7.1.23; AltName: Full=Poly(P)/ATP NAD kinase;
CATALYTIC ACTIVITY: ATP + NAD(+) = ADP + NADP(+).
COFACTOR: Divalent metal cations.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.3 mM for ATP; KM=0.54 mM for NAD; Vmax=6.7 umol/min/mg enzyme; pH dependence: Optimum pH is 7.5; Temperature dependence: Optimum temperature is 55 degrees Celsius;
INTERACTION: P63104:YWHAZ; NbExp=1; IntAct=EBI-743949, EBI-347088;
TISSUE SPECIFICITY: Widely expressed but not detected in skeletal muscle.
SIMILARITY: Belongs to the NAD kinase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 74.98 RPKM in Whole Blood
Total median expression: 1081.39 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -115.40222-0.520 Picture PostScript Text
3' UTR -601.801672-0.360 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016064 - ATP-NAD_kinase_PpnK-typ
IPR017438 - ATP-NAD_kinase_PpnK-typ_a/b
IPR017437 - ATP-NAD_kinase_PpnK-typ_all-b
IPR002504 - polyP/ATP_NADK_prd

Pfam Domains:
PF01513 - ATP-NAD kinase N-terminal domain
PF20143 - ATP-NAD kinase C-terminal domain

SCOP Domains:
111331 - NAD kinase/diacylglycerol kinase-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3PFN - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O95544
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003951 NAD+ kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006741 NADP biosynthetic process
GO:0008152 metabolic process
GO:0016310 phosphorylation
GO:0019674 NAD metabolic process
GO:0046034 ATP metabolic process

Cellular Component:
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AF250320 - Homo sapiens HT029 mRNA, complete cds.
BC001709 - Homo sapiens NAD kinase, mRNA (cDNA clone MGC:1900 IMAGE:3542247), complete cds.
AK023114 - Homo sapiens cDNA FLJ13052 fis, clone NT2RP3001449.
AK290161 - Homo sapiens cDNA FLJ78307 complete cds, highly similar to Homo sapiens NAD kinase (NADK), mRNA.
AK291599 - Homo sapiens cDNA FLJ77769 complete cds, highly similar to Homo sapiens NAD kinase, mRNA.
AK291728 - Homo sapiens cDNA FLJ78247 complete cds, highly similar to Homo sapiens NAD kinase, mRNA.
AF070630 - Homo sapiens clone 24497 mRNA sequence.
BC022063 - Homo sapiens NAD kinase, mRNA (cDNA clone IMAGE:2989769), partial cds.
JD351398 - Sequence 332422 from Patent EP1572962.
JD448115 - Sequence 429139 from Patent EP1572962.
BC024219 - Homo sapiens NAD kinase, mRNA (cDNA clone IMAGE:3845203), partial cds.
AK095043 - Homo sapiens cDNA FLJ37724 fis, clone BRHIP2019589, weakly similar to FORKHEAD BOX PROTEIN D2.
BX647173 - Homo sapiens mRNA; cDNA DKFZp686L22239 (from clone DKFZp686L22239).
JD399467 - Sequence 380491 from Patent EP1572962.
JD154770 - Sequence 135794 from Patent EP1572962.
JD353400 - Sequence 334424 from Patent EP1572962.
JD396871 - Sequence 377895 from Patent EP1572962.
JD128449 - Sequence 109473 from Patent EP1572962.
JD419357 - Sequence 400381 from Patent EP1572962.
JD100799 - Sequence 81823 from Patent EP1572962.
JD125018 - Sequence 106042 from Patent EP1572962.
JD390055 - Sequence 371079 from Patent EP1572962.
JD390054 - Sequence 371078 from Patent EP1572962.
JD519180 - Sequence 500204 from Patent EP1572962.
JD140757 - Sequence 121781 from Patent EP1572962.
JD412245 - Sequence 393269 from Patent EP1572962.
JD358585 - Sequence 339609 from Patent EP1572962.
JD296149 - Sequence 277173 from Patent EP1572962.
JD511978 - Sequence 493002 from Patent EP1572962.
JD097371 - Sequence 78395 from Patent EP1572962.
JD053170 - Sequence 34194 from Patent EP1572962.
JD211573 - Sequence 192597 from Patent EP1572962.
JD153909 - Sequence 134933 from Patent EP1572962.
AK296722 - Homo sapiens cDNA FLJ54695 complete cds, highly similar to NAD kinase (EC 2.7.1.23).
JD200188 - Sequence 181212 from Patent EP1572962.
JD301823 - Sequence 282847 from Patent EP1572962.
JD117743 - Sequence 98767 from Patent EP1572962.
JD132340 - Sequence 113364 from Patent EP1572962.
JD328718 - Sequence 309742 from Patent EP1572962.
JD071198 - Sequence 52222 from Patent EP1572962.
JD164301 - Sequence 145325 from Patent EP1572962.
AK307645 - Homo sapiens cDNA, FLJ97593.
JD048259 - Sequence 29283 from Patent EP1572962.
AY090771 - Homo sapiens NAD kinase mRNA, complete cds.
KJ899498 - Synthetic construct Homo sapiens clone ccsbBroadEn_08892 NADK gene, encodes complete protein.
KJ905530 - Synthetic construct Homo sapiens clone ccsbBroadEn_15141 NADK gene, encodes complete protein.
DQ576235 - Homo sapiens piRNA piR-44347, complete sequence.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
NADPHOS-DEPHOS-PWY-1 - NAD phosphorylation and transhydrogenation

Reactome (by CSHL, EBI, and GO)

Protein O95544 (Reactome details) participates in the following event(s):

R-HSA-197198 NADK:Zn2+ tetramer phosphorylates NAD+ to NADP+
R-HSA-196807 Nicotinate metabolism
R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
R-HSA-196854 Metabolism of vitamins and cofactors
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A6NNN3, A8K296, B7Z434, ENST00000341426.1, ENST00000341426.2, ENST00000341426.3, ENST00000341426.4, ENST00000341426.5, ENST00000341426.6, ENST00000341426.7, ENST00000341426.8, F5GXR5, NADK_HUMAN, NM_023018, O95544, Q5QPS4, Q9H2P2, Q9H931, uc317wah.1, uc317wah.2
UCSC ID: ENST00000341426.9_7
RefSeq Accession: NM_023018.5
Protein: O95544 (aka NADK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.