Human Gene MYLK3 (ENST00000394809.9_11) from GENCODE V47lift37

JavaScript is disabled in your web browser

You must have JavaScript enabled in your web browser to use the Genome Browser

Description: myosin light chain kinase 3, transcript variant 1 (from RefSeq NM_182493.3)
Gencode Transcript: ENST00000394809.9_11
Gencode Gene: ENSG00000140795.13_12
Transcript (Including UTRs)
Position: hg19 chr16:46,736,194-46,782,256 Size: 46,063 Total Exon Count: 13 Strand: -
Coding Region
Position: hg19 chr16:46,741,616-46,782,105 Size: 40,490 Coding Exon Count: 13

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr16:46,736,194-46,782,256)			mRNA (may differ from genome)		Protein (819 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	UniProtKB	Wikipedia
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: MYLK3_HUMAN DESCRIPTION: RecName: Full=Myosin light chain kinase 3; EC=2.7.11.18; AltName: Full=Cardiac-MyBP-C-associated Ca/CaM kinase; Short=Cardiac-MLCK; FUNCTION: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity). CATALYTIC ACTIVITY: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. COFACTOR: Magnesium (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). TISSUE SPECIFICITY: Restricted to heart. PTM: Phosphorylated on serine residues (By similarity). SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SIMILARITY: Contains 1 protein kinase domain. SEQUENCE CAUTION: Sequence=AAI09098.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAC42766.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C547126 AZM551248 D001564 Benzo(a)pyrene D004390 Chlorpyrifos D003300 Copper D004147 Dioxins D004958 Estradiol D010656 Phenylephrine D014028 Tobacco Smoke Pollution D014635 Valproic Acid

Common Gene Haplotype Alleles


	Press "+" in the title bar above to open this section.

RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 19.08 RPKM in Heart - Atrial Appendage Total median expression: 43.59 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


	Press "+" in the title bar above to open this section.

mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-51.20	151	-0.339	Picture	PostScript	Text
3' UTR	-1954.20	5422	-0.360	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR020636 - Ca/CaM-dep_Ca-dep_prot_Kinase
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF03109 - ABC1 atypical kinase-like domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q32MK0


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD	Ensembl

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0000166 nucleotide binding GO:0004672 protein kinase activity GO:0004674 protein serine/threonine kinase activity GO:0004683 calmodulin-dependent protein kinase activity GO:0004687 myosin light chain kinase activity GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity Biological Process: GO:0002528 regulation of vascular permeability involved in acute inflammatory response GO:0006468 protein phosphorylation GO:0016310 phosphorylation GO:0018105 peptidyl-serine phosphorylation GO:0018107 peptidyl-threonine phosphorylation GO:0035556 intracellular signal transduction GO:0045214 sarcomere organization GO:0048769 sarcomerogenesis GO:0055003 cardiac myofibril assembly GO:0060298 positive regulation of sarcomere organization GO:0071347 cellular response to interleukin-1 Cellular Component: GO:0005622 intracellular GO:0005737 cytoplasm GO:0005829 cytosol GO:0015629 actin cytoskeleton

Descriptions from all associated GenBank mRNAs


	AK299443 - Homo sapiens cDNA FLJ50066 complete cds, highly similar to Homo sapiens cardiac-MyBP-C associated Ca/CaM kinase (MLCK), mRNA. JD518087 - Sequence 499111 from Patent EP1572962. JD364587 - Sequence 345611 from Patent EP1572962. JD179186 - Sequence 160210 from Patent EP1572962. JD430475 - Sequence 411499 from Patent EP1572962. JD394717 - Sequence 375741 from Patent EP1572962. JD365215 - Sequence 346239 from Patent EP1572962. JD451708 - Sequence 432732 from Patent EP1572962. JD534486 - Sequence 515510 from Patent EP1572962. JD292443 - Sequence 273467 from Patent EP1572962. JD092047 - Sequence 73071 from Patent EP1572962. JD365214 - Sequence 346238 from Patent EP1572962. JD254666 - Sequence 235690 from Patent EP1572962. JD360642 - Sequence 341666 from Patent EP1572962. JD308135 - Sequence 289159 from Patent EP1572962. JD308134 - Sequence 289158 from Patent EP1572962. JD301095 - Sequence 282119 from Patent EP1572962. JD567063 - Sequence 548087 from Patent EP1572962. JD179064 - Sequence 160088 from Patent EP1572962. JD421446 - Sequence 402470 from Patent EP1572962. JD514910 - Sequence 495934 from Patent EP1572962. JD179063 - Sequence 160087 from Patent EP1572962. JD249636 - Sequence 230660 from Patent EP1572962. JD148199 - Sequence 129223 from Patent EP1572962. JD463722 - Sequence 444746 from Patent EP1572962. JD478467 - Sequence 459491 from Patent EP1572962. JD543132 - Sequence 524156 from Patent EP1572962. JD249635 - Sequence 230659 from Patent EP1572962. JD148198 - Sequence 129222 from Patent EP1572962. JD404869 - Sequence 385893 from Patent EP1572962. JD543131 - Sequence 524155 from Patent EP1572962. JD478466 - Sequence 459490 from Patent EP1572962. JD543130 - Sequence 524154 from Patent EP1572962. JD376068 - Sequence 357092 from Patent EP1572962. JD497800 - Sequence 478824 from Patent EP1572962. JD160182 - Sequence 141206 from Patent EP1572962. AJ247087 - Homo sapiens mRNA for myosin light chain kinase (MLCK). BC109096 - Homo sapiens cDNA clone IMAGE:40034721, containing frame-shift errors. BC109097 - Homo sapiens myosin light chain kinase 3, mRNA (cDNA clone MGC:126320 IMAGE:40034724), complete cds. JD424047 - Sequence 405071 from Patent EP1572962. JD315554 - Sequence 296578 from Patent EP1572962. KJ903575 - Synthetic construct Homo sapiens clone ccsbBroadEn_12969 MYLK3 gene, encodes complete protein. AB451455 - Homo sapiens MYLK3 mRNA for MLCK protein, partial cds, clone: FLJ09002AAAF. BC039103 - Homo sapiens myosin light chain kinase 3, mRNA (cDNA clone IMAGE:4831102), with apparent retained intron. JD049351 - Sequence 30375 from Patent EP1572962. JD440315 - Sequence 421339 from Patent EP1572962. JD056244 - Sequence 37268 from Patent EP1572962. JD340550 - Sequence 321574 from Patent EP1572962.

Other Names for This Gene


	Alternate Gene Symbols: B5BUL9, B7Z5U8, ENST00000394809.1, ENST00000394809.2, ENST00000394809.3, ENST00000394809.4, ENST00000394809.5, ENST00000394809.6, ENST00000394809.7, ENST00000394809.8, MLCK, MYLK3_HUMAN, NM_182493, Q32MK0, Q32MK1, Q96DV1, uc318wxr.1, uc318wxr.2 UCSC ID: ENST00000394809.9_11 RefSeq Accession: NM_182493.3 Protein: Q32MK0 (aka MYLK3_HUMAN)

Gene Model Information


	Click here for a detailed description of the fields of the table above.

Methods, Credits, and Use Restrictions


	Click here for details on how this gene model was made and data restrictions if any.