Human Gene MGST1 (ENST00000396210.8_4) from GENCODE V47lift37
  Description: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. (from UniProt P10620)
Gencode Transcript: ENST00000396210.8_4
Gencode Gene: ENSG00000008394.13_12
Transcript (Including UTRs)
   Position: hg19 chr12:16,500,579-16,517,344 Size: 16,766 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr12:16,507,187-16,516,975 Size: 9,789 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:16,500,579-16,517,344)mRNA (may differ from genome)Protein (155 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MalacardsMGIPubMedReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: MGST1_HUMAN
DESCRIPTION: RecName: Full=Microsomal glutathione S-transferase 1; Short=Microsomal GST-1; EC=2.5.1.18; AltName: Full=Microsomal GST-I;
FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
CATALYTIC ACTIVITY: RX + glutathione = HX + R-S-glutathione.
ENZYME REGULATION: Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).
SUBUNIT: Homotrimer; The trimer binds only one molecule of glutathione (By similarity).
INTERACTION: Q8WX92:COBRA1; NbExp=2; IntAct=EBI-2691601, EBI-347721;
SUBCELLULAR LOCATION: Microsome (By similarity). Mitochondrion outer membrane; Peripheral membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in liver.
PTM: Peroxynitrite induces nitration at Tyr-93 which activates the enzyme (By similarity).
SIMILARITY: Belongs to the MAPEG family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mgst1/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MGST1
Diseases sorted by gene-association score: megalencephalic leukoencephalopathy with subcortical cysts (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 132.31 RPKM in Adrenal Gland
Total median expression: 934.74 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.2088-0.207 Picture PostScript Text
3' UTR -60.50369-0.164 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023352 - MAPEG-like_dom
IPR001129 - Membr-assoc_MAPEG

Pfam Domains:
PF01124 - MAPEG family

SCOP Domains:
161084 - MAPEG domain-like

ModBase Predicted Comparative 3D Structure on P10620
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004364 glutathione transferase activity
GO:0004602 glutathione peroxidase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0043295 glutathione binding

Biological Process:
GO:0006805 xenobiotic metabolic process
GO:0010243 response to organonitrogen compound
GO:0032496 response to lipopolysaccharide
GO:0033327 Leydig cell differentiation
GO:0042493 response to drug
GO:0043312 neutrophil degranulation
GO:0055114 oxidation-reduction process
GO:0070207 protein homotrimerization
GO:0071449 cellular response to lipid hydroperoxide
GO:0098869 cellular oxidant detoxification
GO:1901687 glutathione derivative biosynthetic process

Cellular Component:
GO:0005634 nucleus
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0005778 peroxisomal membrane
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0035577 azurophil granule membrane
GO:0043231 intracellular membrane-bounded organelle
GO:0045177 apical part of cell


-  Descriptions from all associated GenBank mRNAs
  AK291148 - Homo sapiens cDNA FLJ76401 complete cds, highly similar to Homo sapiens microsomal glutathione S-transferase 1 (MGST1), transcript variant 1b, mRNA.
AK309707 - Homo sapiens cDNA, FLJ99748.
BC005923 - Homo sapiens microsomal glutathione S-transferase 1, mRNA (cDNA clone MGC:14525 IMAGE:4297300), complete cds.
BC056863 - Homo sapiens cDNA clone IMAGE:5196064, partial cds.
J03746 - Human glutathione S-transferase mRNA, complete cds.
CR407629 - Homo sapiens full open reading frame cDNA clone RZPDo834D062D for gene MGST1, microsomal glutathione S-transferase 1 complete cds, without stopcodon.
BT006982 - Homo sapiens microsomal glutathione S-transferase 1 mRNA, complete cds.
KJ891615 - Synthetic construct Homo sapiens clone ccsbBroadEn_01009 MGST1 gene, encodes complete protein.
KJ897188 - Synthetic construct Homo sapiens clone ccsbBroadEn_06582 MGST1 gene, encodes complete protein.
BC062473 - Homo sapiens cDNA clone IMAGE:30374677, partial cds.
AK123482 - Homo sapiens cDNA FLJ41488 fis, clone BRTHA2004361.
JD146602 - Sequence 127626 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4061 - glutathione-mediated detoxification I

Reactome (by CSHL, EBI, and GO)

Protein P10620 (Reactome details) participates in the following event(s):

R-HSA-6798739 Exocytosis of azurophil granule membrane proteins
R-HSA-176059 GST trimers transfer GS from GSH to luminal substrates
R-HSA-5423653 MGST trimers transfer GS from GSH to AFXBO and AFNBO
R-HSA-6798695 Neutrophil degranulation
R-HSA-168249 Innate Immune System
R-HSA-156590 Glutathione conjugation
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-168256 Immune System
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K533, ENST00000396210.1, ENST00000396210.2, ENST00000396210.3, ENST00000396210.4, ENST00000396210.5, ENST00000396210.6, ENST00000396210.7, G5EA53, GST12, MGST, MGST1_HUMAN, NR_182294, P10620, uc318ybu.1, uc318ybu.2
UCSC ID: ENST00000396210.8_4
RefSeq Accession: NM_020300.5
Protein: P10620 (aka MGST1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.