Human Gene MED16 (ENST00000325464.6_9) from GENCODE V47lift37
  Description: mediator complex subunit 16 (from RefSeq NM_005481.3)
Gencode Transcript: ENST00000325464.6_9
Gencode Gene: ENSG00000175221.16_21
Transcript (Including UTRs)
   Position: hg19 chr19:867,963-893,187 Size: 25,225 Total Exon Count: 16 Strand: -
Coding Region
   Position: hg19 chr19:868,101-891,131 Size: 23,031 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:867,963-893,187)mRNA (may differ from genome)Protein (877 aa)
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-  Comments and Description Text from UniProtKB
  ID: MED16_HUMAN
DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 16; AltName: Full=Mediator complex subunit 16; AltName: Full=Thyroid hormone receptor-associated protein 5; AltName: Full=Thyroid hormone receptor-associated protein complex 95 kDa component; Short=Trap95; AltName: Full=Vitamin D3 receptor-interacting protein complex 92 kDa component; Short=DRIP92;
FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Belongs to the Mediator complex subunit 16 family.
SIMILARITY: Contains 5 WD repeats.
CAUTION: It is uncertain whether Met-1 or Met-13 is the initiator.
SEQUENCE CAUTION: Sequence=AAD31087.1; Type=Frameshift; Positions=609;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.80 RPKM in Cervix - Endocervix
Total median expression: 783.31 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -46.00120-0.383 Picture PostScript Text
3' UTR -54.90138-0.398 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021665 - Mediator_Med16
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat
PF11635 - Mediator complex subunit 16

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q9Y2X0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003712 transcription cofactor activity
GO:0003713 transcription coactivator activity
GO:0003824 catalytic activity
GO:0005515 protein binding
GO:0030375 thyroid hormone receptor coactivator activity
GO:0038023 signaling receptor activity
GO:0042809 vitamin D receptor binding
GO:0046966 thyroid hormone receptor binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006366 transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0030518 intracellular steroid hormone receptor signaling pathway
GO:0030521 androgen receptor signaling pathway
GO:0045893 positive regulation of transcription, DNA-templated

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016020 membrane
GO:0016592 mediator complex


-  Descriptions from all associated GenBank mRNAs
  BC017282 - Homo sapiens mediator complex subunit 16, mRNA (cDNA clone MGC:29551 IMAGE:4876977), complete cds.
AL137732 - Homo sapiens mRNA; cDNA DKFZp434K0420 (from clone DKFZp434K0420).
BC007853 - Homo sapiens mediator complex subunit 16, mRNA (cDNA clone IMAGE:4298280), partial cds.
BC004554 - Homo sapiens mediator complex subunit 16, mRNA (cDNA clone MGC:10649 IMAGE:4053743), complete cds.
BC011841 - Homo sapiens mediator complex subunit 16, mRNA (cDNA clone MGC:20053 IMAGE:4053664), complete cds.
AF106934 - Homo sapiens vitamin D receptor-interacting protein (DRIP92) mRNA, complete cds.
EU392458 - Homo sapiens mediator complex subunit MED16 variant MED16_i12 (MED16) mRNA, complete cds, alternatively spliced.
EU392464 - Homo sapiens mediator complex subunit MED16 variant MED16_i18 (MED16) mRNA, complete cds, alternatively spliced.
EU392465 - Homo sapiens mediator complex subunit MED16 variant MED16_i19 (MED16) mRNA, complete cds, alternatively spliced.
EU392467 - Homo sapiens mediator complex subunit MED16 variant MED16_i21 (MED16) mRNA, complete cds, alternatively spliced.
EU392469 - Homo sapiens mediator complex subunit MED16 variant MED16_i23 (MED16) mRNA, complete cds, alternatively spliced.
EU392470 - Homo sapiens mediator complex subunit MED16 variant MED16_i24 (MED16) mRNA, complete cds, alternatively spliced.
EU392471 - Homo sapiens mediator complex subunit MED16 variant MED16_i25 (MED16) mRNA, complete cds, alternatively spliced.
EU392472 - Homo sapiens mediator complex subunit MED16 variant MED16_i26 (MED16) mRNA, complete cds, alternatively spliced.
EU392473 - Homo sapiens mediator complex subunit MED16 variant MED16_i27 (MED16) mRNA, complete cds, alternatively spliced.
EU392474 - Homo sapiens mediator complex subunit MED16 variant MED16_i28 (MED16) mRNA, complete cds, alternatively spliced.
EU392475 - Homo sapiens mediator complex subunit MED16 variant MED16_i29 (MED16) mRNA, complete cds, alternatively spliced.
EU392476 - Homo sapiens mediator complex subunit MED16 variant MED16_i30 (MED16) mRNA, complete cds, alternatively spliced.
EU392477 - Homo sapiens mediator complex subunit MED16 variant MED16_i31 (MED16) mRNA, complete cds, alternatively spliced.
EU392478 - Homo sapiens mediator complex subunit MED16 variant MED16_i32 (MED16) mRNA, complete cds, alternatively spliced.
EU392479 - Homo sapiens mediator complex subunit MED16 variant MED16_i33 (MED16) mRNA, complete cds, alternatively spliced.
EU392480 - Homo sapiens mediator complex subunit MED16 variant MED16_i34 (MED16) mRNA, complete cds, alternatively spliced.
EU392481 - Homo sapiens mediator complex subunit MED16 variant MED16_i35 (MED16) mRNA, complete cds, alternatively spliced.
AF121228 - Homo sapiens thyroid hormone receptor-associated protein complex component TRAP95 mRNA, complete cds.
DQ893678 - Synthetic construct clone IMAGE:100006308; FLH188895.01X; RZPDo839H0373D thyroid hormone receptor associated protein 5 (THRAP5) gene, encodes complete protein.
DQ895933 - Synthetic construct Homo sapiens clone IMAGE:100010393; FLH188891.01L; RZPDo839H0363D thyroid hormone receptor associated protein 5 (THRAP5) gene, encodes complete protein.
AB463110 - Synthetic construct DNA, clone: pF1KB8729, Homo sapiens MED16 gene for mediator complex subunit 16, without stop codon, in Flexi system.
EU392448 - Homo sapiens mediator complex subunit MED16 variant MED16_i2 (MED16) mRNA, partial cds, alternatively spliced.
EU392450 - Homo sapiens mediator complex subunit MED16 variant MED16_i4 (MED16) mRNA, partial cds, alternatively spliced.
EU392459 - Homo sapiens mediator complex subunit MED16 variant MED16_i13 (MED16) mRNA, partial cds, alternatively spliced.
EU392466 - Homo sapiens mediator complex subunit MED16 variant MED16_i20 (MED16) mRNA, partial cds, alternatively spliced.
EU392468 - Homo sapiens mediator complex subunit MED16 variant MED16_i22 (MED16) mRNA, partial cds, alternatively spliced.
EU392498 - Homo sapiens mediator complex subunit MED16 variant MED16_i52 (MED16) mRNA, complete sequence, alternatively spliced.
EU392497 - Homo sapiens mediator complex subunit MED16 variant MED16_i51 (MED16) mRNA, complete sequence, alternatively spliced.
DQ572666 - Homo sapiens piRNA piR-40778, complete sequence.
EU392462 - Homo sapiens mediator complex subunit MED16 variant MED16_i16 (MED16) mRNA, partial cds, alternatively spliced.
EU392455 - Homo sapiens mediator complex subunit MED16 variant MED16_i9 (MED16) mRNA, partial cds, alternatively spliced.
EU392461 - Homo sapiens mediator complex subunit MED16 variant MED16_i15 (MED16) mRNA, partial cds, alternatively spliced.
EU392485 - Homo sapiens mediator complex subunit MED16 variant MED16_i39 (MED16) mRNA, complete sequence, alternatively spliced.
EU392488 - Homo sapiens mediator complex subunit MED16 variant MED16_i42 (MED16) mRNA, complete sequence, alternatively spliced.
EU392489 - Homo sapiens mediator complex subunit MED16 variant MED16_i43 (MED16) mRNA, complete sequence, alternatively spliced.
EU392490 - Homo sapiens mediator complex subunit MED16 variant MED16_i44 (MED16) mRNA, complete sequence, alternatively spliced.
EU392496 - Homo sapiens mediator complex subunit MED16 variant MED16_i50 (MED16) mRNA, complete sequence, alternatively spliced.
EU392449 - Homo sapiens mediator complex subunit MED16 variant MED16_i3 (MED16) mRNA, partial cds, alternatively spliced.
EU392451 - Homo sapiens mediator complex subunit MED16 variant MED16_i5 (MED16) mRNA, partial cds, alternatively spliced.
EU392452 - Homo sapiens mediator complex subunit MED16 variant MED16_i6 (MED16) mRNA, partial cds, alternatively spliced.
EU392453 - Homo sapiens mediator complex subunit MED16 variant MED16_i7 (MED16) mRNA, partial cds, alternatively spliced.
EU392454 - Homo sapiens mediator complex subunit MED16 variant MED16_i8 (MED16) mRNA, partial cds, alternatively spliced.
EU392456 - Homo sapiens mediator complex subunit MED16 variant MED16_i10 (MED16) mRNA, partial cds, alternatively spliced.
EU392457 - Homo sapiens mediator complex subunit MED16 variant MED16_i11 (MED16) mRNA, partial cds, alternatively spliced.
EU392460 - Homo sapiens mediator complex subunit MED16 variant MED16_i14 (MED16) mRNA, partial cds, alternatively spliced.
EU392463 - Homo sapiens mediator complex subunit MED16 variant MED16_i17 (MED16) mRNA, partial cds, alternatively spliced.
EU392484 - Homo sapiens mediator complex subunit MED16 variant MED16_i38 (MED16) mRNA, complete sequence, alternatively spliced.
EU392486 - Homo sapiens mediator complex subunit MED16 variant MED16_i40 (MED16) mRNA, complete sequence, alternatively spliced.
EU392487 - Homo sapiens mediator complex subunit MED16 variant MED16_i41 (MED16) mRNA, complete sequence, alternatively spliced.
EU392491 - Homo sapiens mediator complex subunit MED16 variant MED16_i45 (MED16) mRNA, complete sequence, alternatively spliced.
EU392492 - Homo sapiens mediator complex subunit MED16 variant MED16_i46 (MED16) mRNA, complete sequence, alternatively spliced.
EU392493 - Homo sapiens mediator complex subunit MED16 variant MED16_i47 (MED16) mRNA, complete sequence, alternatively spliced.
EU392494 - Homo sapiens mediator complex subunit MED16 variant MED16_i48 (MED16) mRNA, complete sequence, alternatively spliced.
EU392495 - Homo sapiens mediator complex subunit MED16 variant MED16_i49 (MED16) mRNA, complete sequence, alternatively spliced.
DQ589766 - Homo sapiens piRNA piR-56878, complete sequence.
DQ570674 - Homo sapiens piRNA piR-30786, complete sequence.
JD190360 - Sequence 171384 from Patent EP1572962.
DQ591520 - Homo sapiens piRNA piR-58632, complete sequence.
EU392482 - Homo sapiens mediator complex subunit MED16 variant MED16_i36 (MED16) mRNA, complete sequence, alternatively spliced.
EU392483 - Homo sapiens mediator complex subunit MED16 variant MED16_i37 (MED16) mRNA, complete sequence, alternatively spliced.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Y2X0 (Reactome details) participates in the following event(s):

R-HSA-212352 Formation of ARC coactivator complex
R-HSA-212380 Formation of TRAP coactivator complex
R-HSA-212432 Formation of DRIP coactivator complex
R-HSA-381309 PPARG:RXRA heterodimer binds to fatty acid-like ligands
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-1266738 Developmental Biology
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: DRIP92 , ENST00000325464.1, ENST00000325464.2, ENST00000325464.3, ENST00000325464.4, ENST00000325464.5, MED16_HUMAN, NM_005481, Q6PJT2, Q96AD4, Q96I35, Q9Y2X0, Q9Y652, THRAP5, uc317rwo.1, uc317rwo.2
UCSC ID: ENST00000325464.6_9
RefSeq Accession: NM_005481.3
Protein: Q9Y2X0 (aka MED16_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.