ID:MARE1_HUMAN DESCRIPTION: RecName: Full=Microtubule-associated protein RP/EB family member 1; AltName: Full=APC-binding protein EB1; AltName: Full=End-binding protein 1; Short=EB1; FUNCTION: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. SUBUNIT: Homodimer. Heterodimer with MAPRE3. Interacts with DCTN1, DCTN2, DIAPH1, DIAPH2, TERF1 and dynein intermediate chain. Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1. Interacts with CDK5RAP2. Interacts with MACF1 (By similarity). Interacts (via C-terminus) with CLIP1. Interacts with SLAIN2. Interacts with KIF18B; this interaction is required for efficient accumulation of KIF18B at microtubule plus ends. INTERACTION: P25054:APC; NbExp=4; IntAct=EBI-1004115, EBI-727707; Q03001:DST; NbExp=2; IntAct=EBI-1004115, EBI-310758; P54274:TERF1; NbExp=2; IntAct=EBI-1004115, EBI-710997; SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome. TISSUE SPECIFICITY: Ubiquitously expressed. DOMAIN: Composed of two functionally independent domains. The N- terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. SIMILARITY: Belongs to the MAPRE family. SIMILARITY: Contains 1 CH (calponin-homology) domain. SIMILARITY: Contains 1 EB1 C-terminal domain. WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/MAPRE1ID455ch20q11.html";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q15691
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK297273 - Homo sapiens cDNA FLJ52068 complete cds, highly similar to Microtubule-associated protein RP/EB family member 1. BC106068 - Homo sapiens microtubule-associated protein, RP/EB family, member 1, mRNA (cDNA clone MGC:117374 IMAGE:6527006), complete cds. U24166 - Homo sapiens EB1 mRNA, complete cds. BC109281 - Homo sapiens microtubule-associated protein, RP/EB family, member 1, mRNA (cDNA clone MGC:129946 IMAGE:40031641), complete cds. BC040410 - Homo sapiens microtubule-associated protein, RP/EB family, member 1, mRNA (cDNA clone IMAGE:2899942). AK312590 - Homo sapiens cDNA, FLJ92966, Homo sapiens microtubule-associated protein, RP/EB family, member 1(MAPRE1), mRNA. JD559055 - Sequence 540079 from Patent EP1572962. BC128442 - Homo sapiens microtubule-associated protein, RP/EB family, member 1, mRNA (cDNA clone IMAGE:40120302), partial cds. AB463888 - Synthetic construct DNA, clone: pF1KB8147, Homo sapiens MAPRE1 gene for microtubule-associated protein, RP/EB family, member 1, without stop codon, in Flexi system. EU832776 - Synthetic construct Homo sapiens clone HAIB:100067805; DKFZo008B0733 microtubule-associated protein, RP/EB family, member 1 protein (MAPRE1) gene, encodes complete protein. GQ129296 - Synthetic construct Homo sapiens clone HAIB:100068466; DKFZo004B0734 microtubule-associated protein, RP/EB family, member 1 protein (MAPRE1) gene, partial cds. KJ893307 - Synthetic construct Homo sapiens clone ccsbBroadEn_02701 MAPRE1 gene, encodes complete protein. AK310817 - Homo sapiens cDNA, FLJ17859. JD042665 - Sequence 23689 from Patent EP1572962. JD507493 - Sequence 488517 from Patent EP1572962. JD502894 - Sequence 483918 from Patent EP1572962. JD321656 - Sequence 302680 from Patent EP1572962. JD235857 - Sequence 216881 from Patent EP1572962. JD539193 - Sequence 520217 from Patent EP1572962. JD336222 - Sequence 317246 from Patent EP1572962. JD192580 - Sequence 173604 from Patent EP1572962. JD039303 - Sequence 20327 from Patent EP1572962. JD506746 - Sequence 487770 from Patent EP1572962. JD261519 - Sequence 242543 from Patent EP1572962. JD416171 - Sequence 397195 from Patent EP1572962. JD391362 - Sequence 372386 from Patent EP1572962. JD539429 - Sequence 520453 from Patent EP1572962. JD469322 - Sequence 450346 from Patent EP1572962. JD186881 - Sequence 167905 from Patent EP1572962. JD078976 - Sequence 60000 from Patent EP1572962. JD354676 - Sequence 335700 from Patent EP1572962. JD257862 - Sequence 238886 from Patent EP1572962. JD294309 - Sequence 275333 from Patent EP1572962. JD277443 - Sequence 258467 from Patent EP1572962. JD298103 - Sequence 279127 from Patent EP1572962. JD149041 - Sequence 130065 from Patent EP1572962. DQ574454 - Homo sapiens piRNA piR-42566, complete sequence. JD350501 - Sequence 331525 from Patent EP1572962. JD304515 - Sequence 285539 from Patent EP1572962. JD236506 - Sequence 217530 from Patent EP1572962. JD459711 - Sequence 440735 from Patent EP1572962. JD283773 - Sequence 264797 from Patent EP1572962. JD357608 - Sequence 338632 from Patent EP1572962. JD284042 - Sequence 265066 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q15691 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-8852298 GTSE1 binds MAPRE1 (EB1) at microtubule plus ends R-HSA-8852306 Mitotic phosphorylation-induced dissociation of GTSE1 from microtubule plus ends R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-5617833 Cilium Assembly R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-69275 G2/M Transition R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction R-HSA-453274 Mitotic G2-G2/M phases
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
