Human Gene MAP3K15 (ENST00000338883.9_6) from GENCODE V47lift37
  Description: mitogen-activated protein kinase kinase kinase 15 (from RefSeq NM_001001671.4)
Gencode Transcript: ENST00000338883.9_6
Gencode Gene: ENSG00000180815.15_8
Transcript (Including UTRs)
   Position: hg19 chrX:19,378,177-19,533,626 Size: 155,450 Total Exon Count: 29 Strand: -
Coding Region
   Position: hg19 chrX:19,378,867-19,533,379 Size: 154,513 Coding Exon Count: 29 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:19,378,177-19,533,626)mRNA (may differ from genome)Protein (1313 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: M3K15_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase kinase kinase 15; EC=2.7.11.25; AltName: Full=Apoptosis signal-regulating kinase 3; AltName: Full=MAPK/ERK kinase kinase 15; Short=MEK kinase 15; Short=MEKK 15;
FUNCTION: May function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-812, inhibited by phosphorylation at Ser-924 and Ser-994 (By similarity).
TISSUE SPECIFICITY: Isoform 2 and isoform 3 are widely expressed. Isoform 2 highest levels are observed in fetal brain, and isoform 3 highest levels in pancreas, peripheral blood leukocytes, fetal brain and spleen.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.45 RPKM in Adrenal Gland
Total median expression: 20.39 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -120.70247-0.489 Picture PostScript Text
3' UTR -190.60690-0.276 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR025136 - DUF4071
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR013761 - SAM/pointed
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF13281 - MAP3K TRAFs-binding domain
PF19039 - ASK kinase PH domain

SCOP Domains:
48452 - TPR-like
47769 - SAM/Pointed domain
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q6ZN16
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
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 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000165 MAPK cascade
GO:0000186 activation of MAPKK activity
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation

Cellular Component:
GO:0005622 intracellular


-  Descriptions from all associated GenBank mRNAs
  AK131477 - Homo sapiens cDNA FLJ16645 fis, clone TESTI4031745, moderately similar to Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.1.-).
AK131412 - Homo sapiens cDNA FLJ16518 fis, clone NT2RI3007443, moderately similar to MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5 (EC 2.7.1.-).
LF211714 - JP 2014500723-A/19217: Polycomb-Associated Non-Coding RNAs.
AK122632 - Homo sapiens cDNA FLJ16053 fis, clone PLACE6019385, highly similar to Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mRNA.
LF382987 - JP 2014500723-A/190490: Polycomb-Associated Non-Coding RNAs.
MA618564 - JP 2018138019-A/190490: Polycomb-Associated Non-Coding RNAs.
MA447291 - JP 2018138019-A/19217: Polycomb-Associated Non-Coding RNAs.
JD407856 - Sequence 388880 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A2AI49, A2AI50, A6NJ61, ASK3, ENST00000338883.1, ENST00000338883.2, ENST00000338883.3, ENST00000338883.4, ENST00000338883.5, ENST00000338883.6, ENST00000338883.7, ENST00000338883.8, M3K15_HUMAN, NM_001001671, Q5JPR4, Q6ZMV3, Q6ZN16, uc317vdz.1, uc317vdz.2
UCSC ID: ENST00000338883.9_6
RefSeq Accession: NM_001001671.4
Protein: Q6ZN16 (aka M3K15_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.