Human Gene LDHAL6A (ENST00000280706.3_4) from GENCODE V47lift37
  Description: lactate dehydrogenase A like 6A, transcript variant 1 (from RefSeq NM_144972.5)
Gencode Transcript: ENST00000280706.3_4
Gencode Gene: ENSG00000166800.11_8
Transcript (Including UTRs)
   Position: hg19 chr11:18,477,403-18,501,148 Size: 23,746 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr11:18,478,228-18,500,417 Size: 22,190 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:18,477,403-18,501,148)mRNA (may differ from genome)Protein (332 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: LDH6A_HUMAN
DESCRIPTION: RecName: Full=L-lactate dehydrogenase A-like 6A; EC=1.1.1.27;
FUNCTION: Displays an lactate dehydrogenase activity. Significantly increases the transcriptional activity of JUN, when overexpressed.
CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)- lactate from pyruvate: step 1/1.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Testis-specific.
SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.29 RPKM in Testis
Total median expression: 7.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -315.00825-0.382 Picture PostScript Text
3' UTR -183.40731-0.251 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001557 - L-lactate/malate_DH
IPR011304 - L-lactate_DH
IPR018177 - L-lactate_DH_AS
IPR022383 - Lactate/malate_DH_C
IPR001236 - Lactate/malate_DH_N
IPR015955 - Lactate_DH/Glyco_Ohase_4_C
IPR016040 - NAD(P)-bd_dom

Pfam Domains:
PF00056 - lactate/malate dehydrogenase, NAD binding domain
PF02866 - lactate/malate dehydrogenase, alpha/beta C-terminal domain

SCOP Domains:
88946 - Sigma2 domain of RNA polymerase sigma factors
69572 - Activating enzymes of the ubiquitin-like proteins
51735 - NAD(P)-binding Rossmann-fold domains
51984 - MurCD N-terminal domain
56327 - LDH C-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q6ZMR3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
 Gene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004459 L-lactate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0019752 carboxylic acid metabolic process
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005737 cytoplasm
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC014340 - Homo sapiens lactate dehydrogenase A-like 6A, mRNA (cDNA clone MGC:23940 IMAGE:3935569), complete cds.
AY581313 - Homo sapiens lactate dehydrogenase mRNA, complete cds.
AK131523 - Homo sapiens cDNA FLJ43406 fis, clone OCBBF2019823, highly similar to Homo sapiens lactate dehydrogenase A -like (LDHL).
KJ903921 - Synthetic construct Homo sapiens clone ccsbBroadEn_13315 LDHAL6A gene, encodes complete protein.
JD289125 - Sequence 270149 from Patent EP1572962.
JD051203 - Sequence 32227 from Patent EP1572962.
JD051203 - Sequence 32227 from Patent EP1572962.
JD380679 - Sequence 361703 from Patent EP1572962.
JD359402 - Sequence 340426 from Patent EP1572962.
DQ598762 - Homo sapiens piRNA piR-36828, complete sequence.
JD311728 - Sequence 292752 from Patent EP1572962.
JD081529 - Sequence 62553 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q6ZMR3 (Reactome details) participates in the following event(s):

R-HSA-70510 LDH tetramer oxidises LACT to PYR
R-HSA-71849 LDH tetramer reduces PYR to LACT
R-HSA-70268 Pyruvate metabolism
R-HSA-71406 Pyruvate metabolism and Citric Acid (TCA) cycle
R-HSA-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: D3DQY5, ENST00000280706.1, ENST00000280706.2, LDH6A_HUMAN, LDHL2, NM_144972, Q6ZMR3, uc317juq.1, uc317juq.2
UCSC ID: ENST00000280706.3_4
RefSeq Accession: NM_144972.5
Protein: Q6ZMR3 (aka LDH6A_HUMAN or LD6A_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.