ID:KIF2C_HUMAN DESCRIPTION: RecName: Full=Kinesin-like protein KIF2C; AltName: Full=Kinesin-like protein 6; AltName: Full=Mitotic centromere-associated kinesin; Short=MCAK; FUNCTION: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. SUBUNIT: Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity). Nucleus (By similarity). Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGOL2. TISSUE SPECIFICITY: Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. DEVELOPMENTAL STAGE: Isoform 2 is expressed in fetal testis. DOMAIN: The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. SIMILARITY: Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. SIMILARITY: Contains 1 kinesin-motor domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q99661
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AB264115 - Homo sapiens MCAK/KIF2c V1 mRNA for mitotic-centromere-associated kinesin/Kinesin family member 2c V1, complete cds. HM005691 - Homo sapiens clone HTL-T-68n testis tissue sperm-binding protein Li 68n mRNA, complete cds. LF383899 - JP 2014500723-A/191402: Polycomb-Associated Non-Coding RNAs. AK312606 - Homo sapiens cDNA, FLJ92983. AK300756 - Homo sapiens cDNA FLJ54088 complete cds, highly similar to Kinesin-like protein KIF2C. U63743 - Homo sapiens mitotic centromere-associated kinesin mRNA, complete cds. BC008764 - Homo sapiens kinesin family member 2C, mRNA (cDNA clone MGC:1266 IMAGE:3347571), complete cds. BC014924 - Homo sapiens kinesin family member 2C, mRNA (cDNA clone MGC:17895 IMAGE:3909438), complete cds. AK290688 - Homo sapiens cDNA FLJ75664 complete cds, highly similar to Homo sapiens kinesin family member 2C (KIF2C), mRNA. CU677119 - Synthetic construct Homo sapiens gateway clone IMAGE:100017467 5' read KIF2C mRNA. AB590244 - Synthetic construct DNA, clone: pFN21AE0500, Homo sapiens KIF2C gene for kinesin family member 2C, without stop codon, in Flexi system. CR450302 - Homo sapiens full open reading frame cDNA clone RZPDo834D061D for gene KIF2C, kinesin family member 2C; complete cds; without stopcodon. BT006759 - Homo sapiens kinesin-like 6 (mitotic centromere-associated kinesin) mRNA, complete cds. DQ893800 - Synthetic construct Homo sapiens clone IMAGE:100008260; FLH164510.01L; RZPDo839H01161D kinesin family member 2C (KIF2C) gene, encodes complete protein. EU176178 - Synthetic construct Homo sapiens clone IMAGE:100006333; FLH164517.01X; RZPDo839F02250D kinesin family member 2C (KIF2C) gene, encodes complete protein. KJ893198 - Synthetic construct Homo sapiens clone ccsbBroadEn_02592 KIF2C gene, encodes complete protein. KJ898328 - Synthetic construct Homo sapiens clone ccsbBroadEn_07722 KIF2C gene, encodes complete protein. AK302267 - Homo sapiens cDNA FLJ53680 complete cds, highly similar to Kinesin-like protein KIF2C. AY026505 - Homo sapiens testis specific mitotic centromere-associated kinesin mRNA, complete cds. MA619476 - JP 2018138019-A/191402: Polycomb-Associated Non-Coding RNAs. AK124208 - Homo sapiens cDNA FLJ42214 fis, clone THYMU2037208. JD490273 - Sequence 471297 from Patent EP1572962. JD244914 - Sequence 225938 from Patent EP1572962. CU679091 - Synthetic construct Homo sapiens gateway clone IMAGE:100020553 5' read KIF2C mRNA. LF356304 - JP 2014500723-A/163807: Polycomb-Associated Non-Coding RNAs. LF356303 - JP 2014500723-A/163806: Polycomb-Associated Non-Coding RNAs. MA591881 - JP 2018138019-A/163807: Polycomb-Associated Non-Coding RNAs. MA591880 - JP 2018138019-A/163806: Polycomb-Associated Non-Coding RNAs. LF356301 - JP 2014500723-A/163804: Polycomb-Associated Non-Coding RNAs. LF356300 - JP 2014500723-A/163803: Polycomb-Associated Non-Coding RNAs. BC032038 - Homo sapiens, clone IMAGE:5166633, mRNA, partial cds. LF356299 - JP 2014500723-A/163802: Polycomb-Associated Non-Coding RNAs. LF206797 - JP 2014500723-A/14300: Polycomb-Associated Non-Coding RNAs. LF358036 - JP 2014500723-A/165539: Polycomb-Associated Non-Coding RNAs. LF356298 - JP 2014500723-A/163801: Polycomb-Associated Non-Coding RNAs. LF356297 - JP 2014500723-A/163800: Polycomb-Associated Non-Coding RNAs. LF358035 - JP 2014500723-A/165538: Polycomb-Associated Non-Coding RNAs. LF356296 - JP 2014500723-A/163799: Polycomb-Associated Non-Coding RNAs. LF358034 - JP 2014500723-A/165537: Polycomb-Associated Non-Coding RNAs. LF356295 - JP 2014500723-A/163798: Polycomb-Associated Non-Coding RNAs. LF356294 - JP 2014500723-A/163797: Polycomb-Associated Non-Coding RNAs. LF356293 - JP 2014500723-A/163796: Polycomb-Associated Non-Coding RNAs. JD519790 - Sequence 500814 from Patent EP1572962. JD355837 - Sequence 336861 from Patent EP1572962. JD056057 - Sequence 37081 from Patent EP1572962. JD069026 - Sequence 50050 from Patent EP1572962. JD110359 - Sequence 91383 from Patent EP1572962. JD454697 - Sequence 435721 from Patent EP1572962. JD406552 - Sequence 387576 from Patent EP1572962. MA591878 - JP 2018138019-A/163804: Polycomb-Associated Non-Coding RNAs. MA591877 - JP 2018138019-A/163803: Polycomb-Associated Non-Coding RNAs. MA591876 - JP 2018138019-A/163802: Polycomb-Associated Non-Coding RNAs. MA442374 - JP 2018138019-A/14300: Polycomb-Associated Non-Coding RNAs. MA593613 - JP 2018138019-A/165539: Polycomb-Associated Non-Coding RNAs. MA591875 - JP 2018138019-A/163801: Polycomb-Associated Non-Coding RNAs. MA591874 - JP 2018138019-A/163800: Polycomb-Associated Non-Coding RNAs. MA593612 - JP 2018138019-A/165538: Polycomb-Associated Non-Coding RNAs. MA591873 - JP 2018138019-A/163799: Polycomb-Associated Non-Coding RNAs. MA593611 - JP 2018138019-A/165537: Polycomb-Associated Non-Coding RNAs. MA591872 - JP 2018138019-A/163798: Polycomb-Associated Non-Coding RNAs. MA591871 - JP 2018138019-A/163797: Polycomb-Associated Non-Coding RNAs. MA591870 - JP 2018138019-A/163796: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q99661 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-990489 Kinesin-13 is a dimer R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-983266 Kinesins bind microtubules R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-983189 Kinesins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-983231 Factors involved in megakaryocyte development and platelet production R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-109582 Hemostasis R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction R-HSA-2132295 MHC class II antigen presentation R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-1280218 Adaptive Immune System R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-168256 Immune System R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-199991 Membrane Trafficking R-HSA-5653656 Vesicle-mediated transport
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
