Human Gene KIF20A (ENST00000394894.8_4) from GENCODE V47lift37
  Description: kinesin family member 20A (from RefSeq NM_005733.3)
Gencode Transcript: ENST00000394894.8_4
Gencode Gene: ENSG00000112984.12_10
Transcript (Including UTRs)
   Position: hg19 chr5:137,514,801-137,523,412 Size: 8,612 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg19 chr5:137,515,370-137,523,102 Size: 7,733 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:137,514,801-137,523,412)mRNA (may differ from genome)Protein (890 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KI20A_HUMAN
DESCRIPTION: RecName: Full=Kinesin-like protein KIF20A; AltName: Full=GG10_2; AltName: Full=Mitotic kinesin-like protein 2; Short=MKlp2; AltName: Full=Rab6-interacting kinesin-like protein; AltName: Full=Rabkinesin-6;
FUNCTION: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
SUBCELLULAR LOCATION: Golgi apparatus (By similarity). Cytoplasm, cytoskeleton, spindle.
PTM: Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
SIMILARITY: Belongs to the kinesin-like protein family.
SIMILARITY: Contains 1 kinesin-motor domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.68 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 37.47 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.30112-0.342 Picture PostScript Text
3' UTR -55.00310-0.177 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019821 - Kinesin_motor_CS
IPR001752 - Kinesin_motor_dom

Pfam Domains:
PF00225 - Kinesin motor domain
PF16796 - Microtubule binding

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on O95235
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003777 microtubule motor activity
GO:0005215 transporter activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0016887 ATPase activity
GO:0019901 protein kinase binding

Biological Process:
GO:0000281 mitotic cytokinesis
GO:0000910 cytokinesis
GO:0000920 cell separation after cytokinesis
GO:0001578 microtubule bundle formation
GO:0007018 microtubule-based movement
GO:0015031 protein transport
GO:0016192 vesicle-mediated transport
GO:0032465 regulation of cytokinesis

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005819 spindle
GO:0005856 cytoskeleton
GO:0005871 kinesin complex
GO:0005874 microtubule
GO:0030496 midbody
GO:0032154 cleavage furrow
GO:0045171 intercellular bridge


-  Descriptions from all associated GenBank mRNAs
  LF211002 - JP 2014500723-A/18505: Polycomb-Associated Non-Coding RNAs.
AK296879 - Homo sapiens cDNA FLJ55710 complete cds, highly similar to Kinesin family member 20A.
AK025790 - Homo sapiens cDNA: FLJ22137 fis, clone HEP20932, highly similar to AF153329 Homo sapiens RAB6KIFL mRNA.
AF153329 - Homo sapiens RAB6KIFL mRNA, complete cds.
BC012999 - Homo sapiens kinesin family member 20A, mRNA (cDNA clone MGC:3571 IMAGE:3604291), complete cds.
AF070672 - Homo sapiens rabkinesin6 mRNA, complete cds.
LF343072 - JP 2014500723-A/150575: Polycomb-Associated Non-Coding RNAs.
KJ892924 - Synthetic construct Homo sapiens clone ccsbBroadEn_02318 KIF20A gene, encodes complete protein.
AB587334 - Synthetic construct DNA, clone: pF1KB8573, Homo sapiens KIF20A gene for kinesin family member 20A, without stop codon, in Flexi system.
AM392783 - Synthetic construct Homo sapiens clone IMAGE:100001834 for hypothetical protein (KIF20A gene).
LF343073 - JP 2014500723-A/150576: Polycomb-Associated Non-Coding RNAs.
LF343074 - JP 2014500723-A/150577: Polycomb-Associated Non-Coding RNAs.
AK024804 - Homo sapiens cDNA: FLJ21151 fis, clone CAS09579, highly similar to AF070672 Homo sapiens rabkinesin6 mRNA.
LF343076 - JP 2014500723-A/150579: Polycomb-Associated Non-Coding RNAs.
LF343077 - JP 2014500723-A/150580: Polycomb-Associated Non-Coding RNAs.
LF343078 - JP 2014500723-A/150581: Polycomb-Associated Non-Coding RNAs.
JD250100 - Sequence 231124 from Patent EP1572962.
JD110629 - Sequence 91653 from Patent EP1572962.
MA578649 - JP 2018138019-A/150575: Polycomb-Associated Non-Coding RNAs.
MA578650 - JP 2018138019-A/150576: Polycomb-Associated Non-Coding RNAs.
MA578651 - JP 2018138019-A/150577: Polycomb-Associated Non-Coding RNAs.
MA578653 - JP 2018138019-A/150579: Polycomb-Associated Non-Coding RNAs.
MA578654 - JP 2018138019-A/150580: Polycomb-Associated Non-Coding RNAs.
MA578655 - JP 2018138019-A/150581: Polycomb-Associated Non-Coding RNAs.
MA446579 - JP 2018138019-A/18505: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O95235 (Reactome details) participates in the following event(s):

R-HSA-156723 Regulation of KIF20A (MKL2) by phosphorylation
R-HSA-984648 Kinesin-6 and RACGAP1 form the heterotetrameric complex centralspindlin
R-HSA-983266 Kinesins bind microtubules
R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules
R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs
R-HSA-68884 Mitotic Telophase/Cytokinesis
R-HSA-68886 M Phase
R-HSA-983189 Kinesins
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-1640170 Cell Cycle
R-HSA-109582 Hemostasis
R-HSA-2132295 MHC class II antigen presentation
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-1280218 Adaptive Immune System
R-HSA-8856688 Golgi-to-ER retrograde transport
R-HSA-168256 Immune System
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: B4DL79, D3DQB6, ENST00000394894.1, ENST00000394894.2, ENST00000394894.3, ENST00000394894.4, ENST00000394894.5, ENST00000394894.6, ENST00000394894.7, KI20A_HUMAN, MKLP2, NM_005733, O95235, RAB6KIFL, uc318wzr.1, uc318wzr.2
UCSC ID: ENST00000394894.8_4
RefSeq Accession: NM_005733.3
Protein: O95235 (aka KI20A_HUMAN or K20A_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.