Human Gene ECPAS (ENST00000684092.1_7) from GENCODE V47lift37
  Description: Ecm29 proteasome adaptor and scaffold, transcript variant 4 (from RefSeq NM_001364930.1)
Gencode Transcript: ENST00000684092.1_7
Gencode Gene: ENSG00000136813.15_20
Transcript (Including UTRs)
   Position: hg19 chr9:114,122,965-114,246,663 Size: 123,699 Total Exon Count: 50 Strand: -
Coding Region
   Position: hg19 chr9:114,124,310-114,235,198 Size: 110,889 Coding Exon Count: 49 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:114,122,965-114,246,663)mRNA (may differ from genome)Protein (1839 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ECM29_HUMAN
DESCRIPTION: RecName: Full=Proteasome-associated protein ECM29 homolog; Short=Ecm29;
FUNCTION: Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis.
SUBUNIT: Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C- terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6.
SUBCELLULAR LOCATION: Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm, cytoskeleton, centrosome. Nucleus. Endosome, multivesicular body. Cytoplasmic vesicle.
SIMILARITY: Contains 27 HEAT repeats.
SEQUENCE CAUTION: Sequence=AAH21127.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAH73240.1; Type=Erroneous gene model prediction; Sequence=CAH73465.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -139.00350-0.397 Picture PostScript Text
3' UTR -340.701345-0.253 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011989 - ARM-like
IPR016024 - ARM-type_fold
IPR026827 - ECM29/GCN1
IPR026826 - ECM29_metazoa
IPR024372 - Proteasome_stabil_ECM29

Pfam Domains:
PF12755 - Vacuolar 14 Fab1-binding region
PF13001 - Proteasome stabiliser

SCOP Domains:
48371 - ARM repeat
48431 - Lipovitellin-phosvitin complex, superhelical domain
48464 - ENTH/VHS domain

ModBase Predicted Comparative 3D Structure on Q5VYK3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGD  WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0005515 protein binding
GO:0032947 protein complex scaffold

Biological Process:
GO:0030433 ER-associated ubiquitin-dependent protein catabolic process
GO:0043248 proteasome assembly

Cellular Component:
GO:0000502 proteasome complex
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005768 endosome
GO:0005769 early endosome
GO:0005770 late endosome
GO:0005771 multivesicular body
GO:0005783 endoplasmic reticulum
GO:0005793 endoplasmic reticulum-Golgi intermediate compartment
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005856 cytoskeleton
GO:0016020 membrane
GO:0030134 ER to Golgi transport vesicle
GO:0030139 endocytic vesicle
GO:0031410 cytoplasmic vesicle
GO:0005802 trans-Golgi network


-  Descriptions from all associated GenBank mRNAs
  AB002366 - Homo sapiens mRNA for KIAA0368 gene, partial cds.
LF383872 - JP 2014500723-A/191375: Polycomb-Associated Non-Coding RNAs.
AK127247 - Homo sapiens cDNA FLJ45314 fis, clone BRHIP3005142, highly similar to Proteasome-associated protein ECM29 homolog.
AK024705 - Homo sapiens cDNA: FLJ21052 fis, clone CAS00490.
AK025689 - Homo sapiens cDNA: FLJ22036 fis, clone HEP08866.
BC172431 - Synthetic construct Homo sapiens clone IMAGE:100069125, MGC:199136 KIAA0368 (KIAA0368) mRNA, encodes complete protein.
MA619449 - JP 2018138019-A/191375: Polycomb-Associated Non-Coding RNAs.
AK124590 - Homo sapiens cDNA FLJ42599 fis, clone BRACE3010416.
AF052139 - Homo sapiens clone 23744 mRNA sequence.
JD228709 - Sequence 209733 from Patent EP1572962.
JD546015 - Sequence 527039 from Patent EP1572962.
JD280756 - Sequence 261780 from Patent EP1572962.
JD528166 - Sequence 509190 from Patent EP1572962.
JD402822 - Sequence 383846 from Patent EP1572962.
JD494264 - Sequence 475288 from Patent EP1572962.
LF354927 - JP 2014500723-A/162430: Polycomb-Associated Non-Coding RNAs.
JD508132 - Sequence 489156 from Patent EP1572962.
JD563463 - Sequence 544487 from Patent EP1572962.
JD235311 - Sequence 216335 from Patent EP1572962.
JD434155 - Sequence 415179 from Patent EP1572962.
JD220465 - Sequence 201489 from Patent EP1572962.
JD078043 - Sequence 59067 from Patent EP1572962.
JD433072 - Sequence 414096 from Patent EP1572962.
JD342682 - Sequence 323706 from Patent EP1572962.
JD202806 - Sequence 183830 from Patent EP1572962.
JD521390 - Sequence 502414 from Patent EP1572962.
JD561985 - Sequence 543009 from Patent EP1572962.
JD315816 - Sequence 296840 from Patent EP1572962.
BC021127 - Homo sapiens KIAA0368, mRNA (cDNA clone IMAGE:4335177), complete cds.
AK027063 - Homo sapiens cDNA: FLJ23410 fis, clone HEP20445.
LF354928 - JP 2014500723-A/162431: Polycomb-Associated Non-Coding RNAs.
LF354929 - JP 2014500723-A/162432: Polycomb-Associated Non-Coding RNAs.
JF432173 - Synthetic construct Homo sapiens clone IMAGE:100073327 KIAA0368 (KIAA0368) gene, encodes complete protein.
KJ904688 - Synthetic construct Homo sapiens clone ccsbBroadEn_14082 KIAA0368-like gene, encodes complete protein.
LF354930 - JP 2014500723-A/162433: Polycomb-Associated Non-Coding RNAs.
LF354931 - JP 2014500723-A/162434: Polycomb-Associated Non-Coding RNAs.
LF354932 - JP 2014500723-A/162435: Polycomb-Associated Non-Coding RNAs.
LF354934 - JP 2014500723-A/162437: Polycomb-Associated Non-Coding RNAs.
LF354936 - JP 2014500723-A/162439: Polycomb-Associated Non-Coding RNAs.
LF354938 - JP 2014500723-A/162441: Polycomb-Associated Non-Coding RNAs.
CU676842 - Synthetic construct Homo sapiens gateway clone IMAGE:100019763 5' read KIAA0368 mRNA.
LF354939 - JP 2014500723-A/162442: Polycomb-Associated Non-Coding RNAs.
LF372481 - JP 2014500723-A/179984: Polycomb-Associated Non-Coding RNAs.
LF354940 - JP 2014500723-A/162443: Polycomb-Associated Non-Coding RNAs.
LF354941 - JP 2014500723-A/162444: Polycomb-Associated Non-Coding RNAs.
LF354943 - JP 2014500723-A/162446: Polycomb-Associated Non-Coding RNAs.
LF354946 - JP 2014500723-A/162449: Polycomb-Associated Non-Coding RNAs.
LF354949 - JP 2014500723-A/162452: Polycomb-Associated Non-Coding RNAs.
MA608058 - JP 2018138019-A/179984: Polycomb-Associated Non-Coding RNAs.
MA590504 - JP 2018138019-A/162430: Polycomb-Associated Non-Coding RNAs.
MA590505 - JP 2018138019-A/162431: Polycomb-Associated Non-Coding RNAs.
MA590506 - JP 2018138019-A/162432: Polycomb-Associated Non-Coding RNAs.
MA590507 - JP 2018138019-A/162433: Polycomb-Associated Non-Coding RNAs.
MA590508 - JP 2018138019-A/162434: Polycomb-Associated Non-Coding RNAs.
MA590509 - JP 2018138019-A/162435: Polycomb-Associated Non-Coding RNAs.
MA590511 - JP 2018138019-A/162437: Polycomb-Associated Non-Coding RNAs.
MA590513 - JP 2018138019-A/162439: Polycomb-Associated Non-Coding RNAs.
MA590515 - JP 2018138019-A/162441: Polycomb-Associated Non-Coding RNAs.
MA590516 - JP 2018138019-A/162442: Polycomb-Associated Non-Coding RNAs.
MA590517 - JP 2018138019-A/162443: Polycomb-Associated Non-Coding RNAs.
MA590518 - JP 2018138019-A/162444: Polycomb-Associated Non-Coding RNAs.
MA590520 - JP 2018138019-A/162446: Polycomb-Associated Non-Coding RNAs.
MA590523 - JP 2018138019-A/162449: Polycomb-Associated Non-Coding RNAs.
MA590526 - JP 2018138019-A/162452: Polycomb-Associated Non-Coding RNAs.
AK122712 - Homo sapiens cDNA FLJ16204 fis, clone CTONG2019232, highly similar to Proteasome-associated protein ECM29 homolog.
AK308313 - Homo sapiens cDNA, FLJ98261.
LF354953 - JP 2014500723-A/162456: Polycomb-Associated Non-Coding RNAs.
LF354954 - JP 2014500723-A/162457: Polycomb-Associated Non-Coding RNAs.
LF354957 - JP 2014500723-A/162460: Polycomb-Associated Non-Coding RNAs.
LF211951 - JP 2014500723-A/19454: Polycomb-Associated Non-Coding RNAs.
LF354975 - JP 2014500723-A/162478: Polycomb-Associated Non-Coding RNAs.
MA590530 - JP 2018138019-A/162456: Polycomb-Associated Non-Coding RNAs.
MA590531 - JP 2018138019-A/162457: Polycomb-Associated Non-Coding RNAs.
MA590534 - JP 2018138019-A/162460: Polycomb-Associated Non-Coding RNAs.
MA447528 - JP 2018138019-A/19454: Polycomb-Associated Non-Coding RNAs.
MA590552 - JP 2018138019-A/162478: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A0A804HJA4, B3KXF2, ECM29, ECM29_HUMAN, ECPAS , KIAA0368, NM_001364930, O15074, Q5VYK3, Q8WU82, uc330mjc.1, uc330mjc.2
UCSC ID: ENST00000684092.1_7
RefSeq Accession: NM_001364929.1
Protein: Q5VYK3 (aka ECM29_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.