Human Gene IFI27 (ENST00000621160.5_8) from GENCODE V47lift37
  Description: interferon alpha inducible protein 27, transcript variant 12 (from RefSeq NM_001366994.1)
Gencode Transcript: ENST00000621160.5_8
Gencode Gene: ENSG00000165949.13_18
Transcript (Including UTRs)
   Position: hg19 chr14:94,577,082-94,583,027 Size: 5,946 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr14:94,578,029-94,582,864 Size: 4,836 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:94,577,082-94,583,027)mRNA (may differ from genome)Protein (119 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IFI27_HUMAN
DESCRIPTION: RecName: Full=Interferon alpha-inducible protein 27, mitochondrial; Short=p27; AltName: Full=Interferon alpha-induced 11.5 kDa protein; AltName: Full=Interferon-stimulated gene 12a protein; Short=ISG12(a); Flags: Precursor;
FUNCTION: Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
SUBCELLULAR LOCATION: Mitochondrion. Membrane; Multi-pass membrane protein (Potential).
INDUCTION: Up-regulated by interferons.
SIMILARITY: Belongs to the IFI6/IFI27 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: IFI27
Diseases sorted by gene-association score: lung clear cell carcinoma (5), esophageal cancer (3), colorectal cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -31.10120-0.259 Picture PostScript Text
3' UTR -35.20163-0.216 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009311 - Interferon-induced_6-16

Pfam Domains:
PF06140 - Interferon-induced 6-16 family

ModBase Predicted Comparative 3D Structure on P40305
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001102 RNA polymerase II activating transcription factor binding
GO:0003674 molecular_function
GO:0005521 lamin binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0006915 apoptotic process
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0046825 regulation of protein export from nucleus
GO:0060337 type I interferon signaling pathway
GO:0097190 apoptotic signaling pathway

Cellular Component:
GO:0005637 nuclear inner membrane
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LF384883 - JP 2014500723-A/192386: Polycomb-Associated Non-Coding RNAs.
AK289535 - Homo sapiens cDNA FLJ78595 complete cds, highly similar to Homo sapiens interferon, alpha-inducible protein 27 (IFI27), mRNA.
AK097084 - Homo sapiens cDNA FLJ39765 fis, clone SPLEN2000222.
AK312089 - Homo sapiens cDNA, FLJ92370, highly similar to Homo sapiens interferon, alpha-inducible protein 27 (IFI27), mRNA.
BX647330 - Homo sapiens mRNA; cDNA DKFZp686F05171 (from clone DKFZp686F05171).
AJ294851 - Homo sapiens partial mRNA for ISG12 protein (p27 gene).
AY126456 - Homo sapiens interferon-induced ISG12c delta isoform (ISG12) mRNA, partial cds; alternatively spliced.
AY126457 - Homo sapiens interferon-induced ISG12-S short isoform (ISG12) mRNA, partial cds; alternatively spliced.
AY126458 - Homo sapiens interferon-induced ISG12-S short isoform (ISG12) mRNA, partial cds; alternatively spliced.
BC015492 - Homo sapiens interferon, alpha-inducible protein 27, mRNA (cDNA clone MGC:8967 IMAGE:3915505), complete cds.
X67325 - Homo sapiens mRNA for ISG12 protein.
KJ891426 - Synthetic construct Homo sapiens clone ccsbBroadEn_00820 IFI27 gene, encodes complete protein.
AB463793 - Synthetic construct DNA, clone: pF1KB8359, Homo sapiens IFI27 gene for interferon, alpha-inducible protein 27, without stop codon, in Flexi system.
AM393107 - Synthetic construct Homo sapiens clone IMAGE:100002549 for hypothetical protein (IFI27 gene).
AM393779 - Synthetic construct Homo sapiens clone IMAGE:100002567 for hypothetical protein (IFI27 gene).
DQ893816 - Synthetic construct Homo sapiens clone IMAGE:100008276; FLH164681.01L; RZPDo839A08157D interferon, alpha-inducible protein 27 (IFI27) gene, encodes complete protein.
BT006781 - Homo sapiens interferon, alpha-inducible protein 27 mRNA, complete cds.
LC420312 - Homo sapiens IFI27 mRNA for interferon alpha-inducible protein 27, complete cds.
AK095039 - Homo sapiens cDNA FLJ37720 fis, clone BRHIP2019149, moderately similar to Interferon, alpha-inducible protein 27.
JD151797 - Sequence 132821 from Patent EP1572962.
JD323154 - Sequence 304178 from Patent EP1572962.
MA620460 - JP 2018138019-A/192386: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P40305 (Reactome details) participates in the following event(s):

R-HSA-909733 Interferon alpha/beta signaling
R-HSA-913531 Interferon Signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A0A087WZF8, A8K0H0, ENST00000621160.1, ENST00000621160.2, ENST00000621160.3, ENST00000621160.4, IFI27 , IFI27_HUMAN, NM_001366994, P40305, Q53YA6, Q6IEC1, Q7Z5R0, Q7Z5R1, Q7Z5R2, Q96BK3, Q9H4B1, uc327tlw.1, uc327tlw.2
UCSC ID: ENST00000621160.5_8
RefSeq Accession: NM_001366994.1
Protein: P40305 (aka IFI27_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.