ID:KAT5_HUMAN DESCRIPTION: RecName: Full=Histone acetyltransferase KAT5; EC=2.3.1.48; AltName: Full=60 kDa Tat-interactive protein; Short=Tip60; AltName: Full=Histone acetyltransferase HTATIP; Short=HIV-1 Tat interactive protein; AltName: Full=Lysine acetyltransferase 5; AltName: Full=cPLA(2)-interacting protein; FUNCTION: Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. CATALYTIC ACTIVITY: Acetyl-CoA + [histone] = CoA + acetyl- [histone]. SUBUNIT: Interacts with the cytoplasmic tail of APP and APBB1/FE65 (By similarity). Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Interacts with ATM. Interacts with PHF17. Interacts with HIV-1 Tat. Interacts with TRIM24 and TRIM68. Forms a complex with SENP6 and UBE2I in response to UV irradiation. INTERACTION: P54253:ATXN1; NbExp=3; IntAct=EBI-399080, EBI-930964; Q9HC52:CBX8; NbExp=2; IntAct=EBI-399080, EBI-712912; O43261:DLEU1; NbExp=2; IntAct=EBI-399080, EBI-710057; O95257:GADD45G; NbExp=2; IntAct=EBI-399080, EBI-448202; Q9P2H0:KIAA1377; NbExp=2; IntAct=EBI-399080, EBI-473176; P29074:PTPN4; NbExp=2; IntAct=EBI-399080, EBI-710431; P04637:TP53; NbExp=3; IntAct=EBI-399080, EBI-366083; SUBCELLULAR LOCATION: Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies. PTM: Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies. PTM: Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. PTM: Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. PTM: Autoacetylation at Lys-327 is required for proper function (By similarity). SIMILARITY: Belongs to the MYST (SAS/MOZ) family. SIMILARITY: Contains 1 C2HC-type zinc finger. SEQUENCE CAUTION: Sequence=AAB02683.1; Type=Erroneous initiation; Note=Translation N-terminally extended; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/htatip/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q92993
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0003713 transcription coactivator activity GO:0004402 histone acetyltransferase activity GO:0005515 protein binding GO:0016407 acetyltransferase activity GO:0016740 transferase activity GO:0016746 transferase activity, transferring acyl groups GO:0046872 metal ion binding GO:0050681 androgen receptor binding GO:0070491 repressing transcription factor binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0000729 DNA double-strand break processing GO:0006260 DNA replication GO:0006302 double-strand break repair GO:0006303 double-strand break repair via nonhomologous end joining GO:0006325 chromatin organization GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0010212 response to ionizing radiation GO:0016032 viral process GO:0016573 histone acetylation GO:0030521 androgen receptor signaling pathway GO:0032703 negative regulation of interleukin-2 production GO:0040008 regulation of growth GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process GO:0045892 negative regulation of transcription, DNA-templated GO:0045893 positive regulation of transcription, DNA-templated GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0071392 cellular response to estradiol stimulus GO:1901985 positive regulation of protein acetylation GO:1904837 beta-catenin-TCF complex assembly
AK313297 - Homo sapiens cDNA, FLJ93809, highly similar to Homo sapiens HIV-1 Tat interactive protein, 60kDa (HTATIP), mRNA. U74667 - Human tat interactive protein (TIP60) mRNA, complete cds. AK225100 - Homo sapiens mRNA for HIV-1 Tat interactive protein, 60kDa isoform 3 variant, clone: CAS07011. AK226002 - Homo sapiens mRNA for HIV-1 Tat interactive protein, 60kDa isoform 3 variant, clone: FCC109C07. LF211172 - JP 2014500723-A/18675: Polycomb-Associated Non-Coding RNAs. BC117167 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone MGC:150776 IMAGE:40125718), complete cds. BC143290 - Homo sapiens cDNA clone IMAGE:9051793, containing frame-shift errors. BC143296 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone MGC:176817 IMAGE:9051800), complete cds. LF344393 - JP 2014500723-A/151896: Polycomb-Associated Non-Coding RNAs. BC093032 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone MGC:110937 IMAGE:30335942), complete cds. AK304664 - Homo sapiens cDNA FLJ56211 complete cds, highly similar to Histone acetyltransferase HTATIP (EC 2.3.1.48). BC064912 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone MGC:70769 IMAGE:6067575), complete cds. U67734 - Human cPLA2 interacting protein mRNA, complete cds. AB463743 - Synthetic construct DNA, clone: pF1KB8185, Homo sapiens HTATIP gene for HIV-1 Tat interacting protein, 60kDa, without stop codon, in Flexi system. HQ258620 - Synthetic construct Homo sapiens clone IMAGE:100073169 K(lysine) acetyltransferase 5 (KAT5), transcript variant 2 (KAT5) gene, encodes complete protein. KJ893065 - Synthetic construct Homo sapiens clone ccsbBroadEn_02459 KAT5 gene, encodes complete protein. LF214056 - JP 2014500723-A/21559: Polycomb-Associated Non-Coding RNAs. BC013211 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone IMAGE:4179558). BC000166 - Homo sapiens K(lysine) acetyltransferase 5, mRNA (cDNA clone IMAGE:2901054), partial cds. U40989 - Human tat interactive protein mRNA, complete cds. AB209813 - Homo sapiens mRNA for HIV-1 Tat interactive protein, 60kDa isoform 3 variant protein. LF344392 - JP 2014500723-A/151895: Polycomb-Associated Non-Coding RNAs. AK130717 - Homo sapiens cDNA FLJ27207 fis, clone SYN03447, highly similar to Homo sapiens HIV-1 Tat interactive protein, 60kDa (HTATIP). LF344390 - JP 2014500723-A/151893: Polycomb-Associated Non-Coding RNAs. LF344389 - JP 2014500723-A/151892: Polycomb-Associated Non-Coding RNAs. JD043287 - Sequence 24311 from Patent EP1572962. JD335145 - Sequence 316169 from Patent EP1572962. JD410749 - Sequence 391773 from Patent EP1572962. JD205313 - Sequence 186337 from Patent EP1572962. JD067617 - Sequence 48641 from Patent EP1572962. JD260081 - Sequence 241105 from Patent EP1572962. JD342944 - Sequence 323968 from Patent EP1572962. JD215022 - Sequence 196046 from Patent EP1572962. JD205696 - Sequence 186720 from Patent EP1572962. JD062716 - Sequence 43740 from Patent EP1572962. JD359953 - Sequence 340977 from Patent EP1572962. MA579970 - JP 2018138019-A/151896: Polycomb-Associated Non-Coding RNAs. MA449633 - JP 2018138019-A/21559: Polycomb-Associated Non-Coding RNAs. MA579969 - JP 2018138019-A/151895: Polycomb-Associated Non-Coding RNAs. MA579967 - JP 2018138019-A/151893: Polycomb-Associated Non-Coding RNAs. MA579966 - JP 2018138019-A/151892: Polycomb-Associated Non-Coding RNAs. MA446749 - JP 2018138019-A/18675: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_pitx2Pathway - Multi-step Regulation of Transcription by Pitx2
Reactome (by CSHL, EBI, and GO)
Protein Q92993 (Reactome details) participates in the following event(s):
R-HSA-3451153 Beta-catenin recruits TRRAP/KAT5 HAT components R-HSA-5686410 BLM mediates dissolution of double Holliday junction R-HSA-5686657 ERCC1:XPF cleaves flaps generated by SSA R-HSA-5693589 D-loop dissociation and strand annealing R-HSA-5693599 Association of Ku heterodimer with ends of DNA double-strand break R-HSA-5682044 KAT5 acetylates ATM at DNA DSBs R-HSA-5693612 MRN complex bound to DNA ends recruits ATM R-HSA-5693540 MRN activates ATM R-HSA-5682018 MRN complex bound to shortened telomeres recruits ATM R-HSA-5693598 ATM phosphorylates NBN R-HSA-5684081 MRN complex binds CDK2 and RBBP8 R-HSA-5693602 ATM recognizes H2AFX-Nucleosomes R-HSA-5684071 RNF4 ubiquitinates MDC1 R-HSA-5685838 CX3 complex binds D-loop structures R-HSA-5693620 D-loop formation mediated by PALB2, BRCA2 and RAD51 R-HSA-5682026 MRN bound to shortened telomeres activates ATM R-HSA-6792712 KAT5 acetylates ATM at shortened telomeres R-HSA-9023860 Histone acetyltransferases are recruited to the TFF1 gene R-HSA-3321975 NuA4 complex acetylates histone H2A, HIST1H4 R-NUL-4551334 NuA4 complex actetylates H2A and H4 R-HSA-3451147 KAT5 HAT complex acetylates TCF4 gene at histone H4 R-HSA-5693583 MDC1 associates with gamma-H2AFX at nuclear foci R-HSA-5683967 EYA1-4 dephosphorylates tyrosine Y142 of H2AFX R-HSA-5683986 APBB1 and MAPK8 bind diphosphorylated H2AFX R-HSA-5693549 ATM phosphorylates histone H2AFX on S139 at DNA DSBs R-HSA-5684096 CDK2 phosphorylates RBBP8 R-HSA-5684140 ATM phosphorylates RBBP8 R-HSA-5693608 Initial resection of double-strand break ends R-HSA-5684108 BRCA1 binds phosphorylated RBBP8 R-HSA-5693542 Association of RPA complexes with ssDNA at resected DNA DSBs R-HSA-5685994 Long-range resection of DNA DSBs by EXO1 or DNA2 R-HSA-5685341 BCDX2 complex stabilizes RAD51 filament R-HSA-5693561 RAD51 binds BRCA2 at resected DNA DSBs R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5685985 EXO1 or DNA2 in complex with BLM or WRN binds initially resected DNA DSBs along with BRIP1 recruitment R-HSA-5693584 Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) R-HSA-5686440 MUS81:EME1,EME2 cleaves D-loop R-HSA-5682967 WHSC1 binds DNA DSBs R-HSA-5693536 ATM phosphorylates MDC1 R-HSA-5683964 ATM phosphorylates EYA1-4 R-HSA-5684875 Binding of ATR:ATRIP to RPA at resected DNA DSBs R-HSA-5682983 ATM phosphorylates WHSC1 R-HSA-5682965 WHSC1 dimethylates histone H4 on lysine K21 at DSBs R-HSA-5682992 KDM4A,B bind H4K20Me2 R-HSA-5685011 ATR activation at DNA DSBs R-HSA-5684887 Activation of CHEK1 at resected DNA DSBs R-HSA-5684882 CHEK1 is recruited to resected DNA DSBs R-HSA-5693580 Association of RAD52 with the RPA complex at resected DNA DSBs R-HSA-5685156 ATR phosphorylates RPA2 R-HSA-5682588 RNF8 binds phosphorylated MDC1 at DNA DSBs R-HSA-5693566 TP53BP1 associates with H4K20Me2 at DNA DSBs R-HSA-5683077 RNF8 and RNF168 ubiquitinate KDM4A,B R-HSA-5693564 Association of RAD51 with RAD52:DNA double-strand break ends R-HSA-5682586 HERC2 and PIAS4 are recruited to DNA DSBs R-HSA-5682629 HERC2 facilitates UBE2N:UBE2V2 binding to RNF8 R-HSA-5682607 PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs R-HSA-5682863 RNF168 binds DNA DSBs R-HSA-5682858 RNF8 and RNF168 ubiquitinate H2AFX R-HSA-5683384 UIMC1 and FAM175A bind DNA DSBs R-HSA-5683405 PPP5C dephosphorylates TP53BP1 R-HSA-5683425 ATM phosphorylates TP53BP1 at DNA DSBs R-HSA-6799332 ATR phosphorylates TP53 R-HSA-5686642 RAD52 promotes single strand annealing at resected DNA DSBs R-HSA-5682598 ATM phosphorylates HERC2 R-HSA-5693551 Phosphorylation of BRCA1-A complex at multiple sites by ATM R-HSA-5683385 Formation of BRCA1-A complex at DNA DSBs R-HSA-5683735 CHEK2 is recruited to DNA DSBs R-HSA-5683801 CHEK2 phosphorylates BRCA1 R-HSA-69891 Phosphorylation and activation of CHEK2 by ATM R-HSA-5684052 PIAS4 SUMOylates MDC1 R-HSA-5686685 RIF1 and PAX1IP bind TP53BP1 at DNA DSBs R-HSA-5686900 TP53BP1 recruits DCLRE1C to ATM R-HSA-5686704 Activated ATM phosphorylates DCLRE1C R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex R-HSA-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates R-HSA-5685938 HDR through Single Strand Annealing (SSA) R-HSA-5693554 Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) R-HSA-5693571 Nonhomologous End-Joining (NHEJ) R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence R-HSA-5693548 Sensing of DNA Double Strand Breaks R-HSA-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-HSA-5693607 Processing of DNA double-strand break ends R-HSA-5693579 Homologous DNA Pairing and Strand Exchange R-HSA-9018519 Estrogen-dependent gene expression R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-5693537 Resolution of D-Loop Structures R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-5693532 DNA Double-Strand Break Repair R-HSA-3214847 HATs acetylate histones R-HSA-2559583 Cellular Senescence R-HSA-5693606 DNA Double Strand Break Response R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-8939211 ESR-mediated signaling R-HSA-195721 Signaling by WNT R-HSA-5693538 Homology Directed Repair R-HSA-73894 DNA Repair R-HSA-3247509 Chromatin modifying enzymes R-HSA-2262752 Cellular responses to stress R-HSA-9006931 Signaling by Nuclear Receptors R-HSA-162582 Signal Transduction R-HSA-4839726 Chromatin organization R-HSA-8953897 Cellular responses to external stimuli R-HSA-69473 G2/M DNA damage checkpoint R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-69481 G2/M Checkpoints R-HSA-5633007 Regulation of TP53 Activity R-HSA-69620 Cell Cycle Checkpoints R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-1640170 Cell Cycle R-HSA-212436 Generic Transcription Pathway R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
