Human Gene GSTK1 (ENST00000358406.10_4) from GENCODE V47lift37
  Description: glutathione S-transferase kappa 1, transcript variant 1 (from RefSeq NM_015917.3)
Gencode Transcript: ENST00000358406.10_4
Gencode Gene: ENSG00000197448.14_7
Transcript (Including UTRs)
   Position: hg19 chr7:142,960,534-142,966,208 Size: 5,675 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr7:142,960,607-142,965,930 Size: 5,324 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:142,960,534-142,966,208)mRNA (may differ from genome)Protein (226 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GSTK1_HUMAN
DESCRIPTION: RecName: Full=Glutathione S-transferase kappa 1; EC=2.5.1.18; AltName: Full=GST 13-13; AltName: Full=GST class-kappa; AltName: Full=GSTK1-1; Short=hGSTK1; AltName: Full=Glutathione S-transferase subunit 13;
FUNCTION: Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
CATALYTIC ACTIVITY: RX + glutathione = HX + R-S-glutathione.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Peroxisome.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: Belongs to the GST superfamily. Kappa family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D016604 Aflatoxin B1
  • D004390 Chlorpyrifos
  • D002994 Clofibrate
  • D016572 Cyclosporine
  • D011794 Quercetin
  • D013749 Tetrachlorodibenzodioxin
  • C516138 (6-(4-(2-piperidin-1-ylethoxy)phenyl))-3-pyridin-4-ylpyrazolo(1,5-a)pyrimidine
  • C049325 1,2-dithiol-3-thione
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C521334 5-(4-dimethylaminobenzoyl)-aminovaleric acid hydroxamate
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 74.96 RPKM in Small Intestine - Terminal Ileum
Total median expression: 1558.71 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.1073-0.440 Picture PostScript Text
3' UTR -72.00278-0.259 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001853 - DSBA-like_thioredoxin_dom
IPR014440 - HCCAis_GSTk
IPR023169 - PDI_central_dom
IPR012336 - Thioredoxin-like_fold

Pfam Domains:
PF01323 - DSBA-like thioredoxin domain

SCOP Domains:
158682 - TerB-like
52833 - Thioredoxin-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1YZX - X-ray MuPIT 3RPN - X-ray MuPIT 3RPP - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9Y2Q3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004364 glutathione transferase activity
GO:0004602 glutathione peroxidase activity
GO:0005102 receptor binding
GO:0005515 protein binding
GO:0015035 protein disulfide oxidoreductase activity
GO:0016740 transferase activity

Biological Process:
GO:0006625 protein targeting to peroxisome
GO:0006749 glutathione metabolic process
GO:0030855 epithelial cell differentiation
GO:0055114 oxidation-reduction process
GO:0098869 cellular oxidant detoxification
GO:1901687 glutathione derivative biosynthetic process

Cellular Component:
GO:0005622 intracellular
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0005777 peroxisome
GO:0005782 peroxisomal matrix
GO:0005829 cytosol
GO:0016020 membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK295592 - Homo sapiens cDNA FLJ57730 complete cds, highly similar to Glutathione S-transferase kappa 1 (EC 2.5.1.18).
AL136938 - Homo sapiens mRNA; cDNA DKFZp586J1023 (from clone DKFZp586J1023); complete cds.
AF087849 - Homo sapiens GSTK1-1 mRNA, complete cds.
AY520571 - Homo sapiens mitochondrial glutathione transferase kappa mRNA, complete cds; nuclear gene for mitochondrial product.
AF068287 - Homo sapiens HDCMD47P mRNA, complete cds.
AF070657 - Homo sapiens glutathione S-transferase subunit 13 homolog mRNA, complete cds.
AK000640 - Homo sapiens cDNA FLJ20633 fis, clone KAT03686, highly similar to AF070657 Homo sapiens glutathione S-transferase subunit 13.
BC001231 - Homo sapiens glutathione S-transferase kappa 1, mRNA (cDNA clone MGC:4882 IMAGE:3460150), complete cds.
AK095263 - Homo sapiens cDNA FLJ37944 fis, clone CTONG2008720, highly similar to Glutathione S-transferase kappa 1 (EC 2.5.1.18).
AK289580 - Homo sapiens cDNA FLJ78056 complete cds.
AK289570 - Homo sapiens cDNA FLJ76598 complete cds, highly similar to Homo sapiens glutathione S-transferase kappa 1, mRNA.
BC063425 - Homo sapiens glutathione S-transferase kappa 1, mRNA (cDNA clone MGC:75013 IMAGE:5218251), complete cds.
AM393732 - Synthetic construct Homo sapiens clone IMAGE:100002105 for hypothetical protein (GSTK1 gene).
BC050715 - Homo sapiens glutathione S-transferase kappa 1, mRNA (cDNA clone MGC:60327 IMAGE:6061265), complete cds.
AK299968 - Homo sapiens cDNA FLJ60871 complete cds, highly similar to Glutathione S-transferase kappa 1 (EC 2.5.1.18).
KJ906491 - Synthetic construct Homo sapiens clone ccsbBroadEn_16161 GSTK1 gene, encodes complete protein.
KJ896136 - Synthetic construct Homo sapiens clone ccsbBroadEn_05530 GSTK1 gene, encodes complete protein.
AB528144 - Synthetic construct DNA, clone: pF1KE0148, Homo sapiens GSTK1 gene for glutathione S-transferase kappa 1, without stop codon, in Flexi system.
CR533445 - Homo sapiens full open reading frame cDNA clone RZPDo834G0717D for gene LOC51064, glutathione S-transferase subunit 13 homolog; complete cds, incl. stopcodon.
BC110600 - Homo sapiens glutathione S-transferase kappa 1, mRNA (cDNA clone IMAGE:40026150), partial cds.
JD165315 - Sequence 146339 from Patent EP1572962.
JD486854 - Sequence 467878 from Patent EP1572962.
JD497332 - Sequence 478356 from Patent EP1572962.
JD401546 - Sequence 382570 from Patent EP1572962.
JD166417 - Sequence 147441 from Patent EP1572962.
JD536531 - Sequence 517555 from Patent EP1572962.
JD335617 - Sequence 316641 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4061 - glutathione-mediated detoxification I

Reactome (by CSHL, EBI, and GO)

Protein Q9Y2Q3 (Reactome details) participates in the following event(s):

R-HSA-9033233 PEX5S,L binds cargo proteins containing PTS1
R-HSA-9033236 PEX5S,L:Cargo binds PEX13:PEX14 of PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Complex)
R-HSA-3301943 GSTK1 dimer transfers GS from GSH to CDNB
R-HSA-9033241 Peroxisomal protein import
R-HSA-392499 Metabolism of proteins
R-HSA-156590 Glutathione conjugation
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B4DIH1, B4DSY2, ENST00000358406.1, ENST00000358406.2, ENST00000358406.3, ENST00000358406.4, ENST00000358406.5, ENST00000358406.6, ENST00000358406.7, ENST00000358406.8, ENST00000358406.9, GSTK1_HUMAN, HDCMD47P, NM_015917, Q6P4H0, Q7Z520, Q9P1S4, Q9Y2Q3, uc318ant.1, uc318ant.2
UCSC ID: ENST00000358406.10_4
RefSeq Accession: NM_015917.3
Protein: Q9Y2Q3 (aka GSTK1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.